scholarly journals Optimised Extraction of Trypsin Inhibitors from Defatted Gac (Momordica cochinchinensis Spreng) Seeds for Production of a Trypsin Inhibitor‐Enriched Freeze Dried Powder

Separations ◽  
2019 ◽  
Vol 6 (1) ◽  
pp. 8 ◽  
Author(s):  
Anh V. Le ◽  
Sophie E. Parks ◽  
Minh H. Nguyen ◽  
Paul D. Roach
Keyword(s):  
Moisture Content ◽  
Freeze Drying ◽  
Trypsin Inhibitors ◽  
Seed Kernel ◽  
Solvent Ratio ◽  
Optimal Conditions ◽  
High Solubility ◽  
High Quality ◽  
Freeze Dried ◽  
Momordica Cochinchinensis

The seeds of the Gac fruit, Momordica cochinchinensis Spreng, are rich in trypsin inhibitors (TIs) but their optimal extraction and the effects of freeze drying are not established. This study aims to (1) compare aqueous solvents (DI water, 0.1 M NaCl, 0.02 M NaOH and ACN)/water/FA, 25:24:1) for extracting TIs from defatted Gac seed kernel powder, (2) to optimise the extraction in terms of solvent, time and material to solvent ratio and (3) to produce a TI-enriched freeze-dried powder (FD-TIP) with good characteristics. Based on the specific TI activity (TIA), the optimal extraction was 1 h using a ratio of 2.0 g of defatted powder in 30 mL of 0.05 M NaCl. The optimisation improved the TIA and specific TIA by 8% and 13%, respectively. The FD-TIP had a high specific TIA (1.57 ± 0.17 mg trypsin/mg protein), although it also contained saponins (43.6 ± 2.3 mg AE/g) and phenolics (10.5 ± 0.3 mg GAE/g). The FD-TIP was likely stable during storage due to its very low moisture content (0.43 ± 0.08%) and water activity (0.18 ± 0.07) and its ability to be easily reconstituted in water due to its high solubility index (92.4 ± 1.5%). Therefore, the optimal conditions for the extraction of TIs from defatted Gac seed kernel powder followed by freeze drying gave a high quality powder in terms of its highly specific TIA and physical properties.

scholarly journals Production and Physical and Physicochemical Characterization Powder in Natura and Freeze-Dried of Moringa Seeds

2019 ◽  
Vol 11 (9) ◽  
pp. 11
Author(s):  
Semirames do N. Silva ◽  
Francisco de Assis C. Almeida ◽  
Josivanda P. Gomes ◽  
Antônio Jackson R. Barroso ◽  
Polyana B. Silva ◽  
...  
Keyword(s):  
Moisture Content ◽  
Water Activity ◽  
Low Temperatures ◽  
Freeze Drying ◽  
Physicochemical Characterization ◽  
High Solubility ◽  
Solubility In Water ◽  
Compressibility Index ◽  
Freeze Dried ◽  
Freeze Dryer

To apply low temperatures, different of other processes, maintain the structure of products and better preserve the thermosensitive components, the freeze-drying has called attention of various researchers. Aimed with the research to produce and characterize the powder in natura obtained by seeds of moringa, elaborate different pastes with addiction of 20, 30, 40 and 50 mL of distilled water, freeze-dry it, characterize it physical and physicochemical and select the best powder. The freeze-drying was produced through a benchtop freeze dryer. After drying it, from the powder were determined the real density, bulk density, compacted density, porosity, compressibility index, Hausner factor, hygroscopicity, solubility, color, moisture content and water activity, ashes, total acidity, pH, proteins, lipids and carbohydrates. The in natura powder was classified as non-hygroscopic, high solubility in water, low moisture content and water activity, high protein, lipids, carbohydrates and low acidity. Freeze-drying was presented as an appropriate method for the preservation of moringa constituents, with a formula selected with the addition of 50 mL of water.

scholarly journals Bioactive Composition, Antioxidant Activity, and Anticancer Potential of Freeze-Dried Extracts from Defatted Gac (Momordica cochinchinensis Spreng) Seeds

Medicines ◽  
2018 ◽  
Vol 5 (3) ◽  
pp. 104 ◽  
Author(s):  
Anh Le ◽  
Tien Huynh ◽  
Sophie Parks ◽  
Minh Nguyen ◽  
Paul Roach
Keyword(s):  
Antioxidant Activity ◽  
Cell Lines ◽  
Trypsin Inhibitors ◽  
Seed Kernel ◽  
Antioxidant Power ◽  
Melanoma Cancer ◽  
Freeze Dried ◽  
Momordica Cochinchinensis ◽  
Highly Correlated ◽  
Water Saturated

Background: Gac (Momordica cochinchinensis Spreng) seeds have long been used in traditional medicine as a remedy for numerous conditions due to a range of bioactive compounds. This study investigated the solvent extraction of compounds that could be responsible for antioxidant activity and anticancer potential. Methods: Defatted Gac seed kernel powder was extracted with different solvents: 100% water, 50% methanol:water, 70% ethanol:water, water saturated butanol, 100% methanol, and 100% ethanol. Trypsin inhibitors, saponins, phenolics, and antioxidant activity using the 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), the 2,2-diphenyl-1-picrylhydrazyl (DPPH) and the ferric reducing antioxidant power (FRAP) assays; and anticancer potential against two melanoma cancer cell lines (MM418C1 and D24) were analysed to determine the best extraction solvents. Results: Water was best for extracting trypsin inhibitors (581.4 ± 18.5 mg trypsin/mg) and reducing the viability of MM418C1 and D24 melanoma cells (75.5 ± 1.3 and 66.9 ± 2.2%, respectively); the anticancer potential against the MM418C1 cells was highly correlated with trypsin inhibitors (r = 0.92, p < 0.05), but there was no correlation between anticancer potential and antioxidant activity. The water saturated butanol had the highest saponins (71.8 ± 4.31 mg aescin equivalents/g), phenolic compounds (20.4 ± 0.86 mg gallic acid equivalents/g), and antioxidant activity, but these measures were not related to anticancer potential. Conclusions: Water yielded a Gac seed extract, rich in trypsin inhibitors, which had high anticancer potential against two melanoma cell lines.

scholarly journals Fortified and freeze-dried kiwi fruit (Actinidia deliciosa): quality and sensory assessment

2020 ◽  
Vol 23 ◽  
Author(s):  
Niladri Chakraborty ◽  
Rajat Chakraborty ◽  
Asit Kumar Saha
Keyword(s):  
Moisture Content ◽  
Freeze Drying ◽  
Raw Materials ◽  
Food Product ◽  
Rice Flour ◽  
Actinidia Deliciosa ◽  
Crude Fibre ◽  
Kiwi Fruit ◽  
Drying Time ◽  
Freeze Dried

Abstract Kiwi fruit (Actinidia deliciosa) (KF) is one of the best fruits available due to its large amount of nutrients. Despite its many health benefits, there are no previous reports on its preparation in other readily ingestible forms. The objective of the present study was to make a new food product from KF. The KF pulp was fortified and blended with several raw materials (such as rice flour and oat flour) using a stepwise short time addition and mixing methodology since this avoids unwanted biochemical and chemical reactions. The blended and reduced moisture KF paste was freeze-dried on a round silver coated steel plate (RSCSP), supplying the heat of sublimation using a newly designed cubic heater. The freeze-drying (FD) time was 4.5 h and the drying kinetics were studied using four established models. The effective moisture diffusivity (Deff) during FD (at 50 °C) was 1.532 x 10-6 m2/s and the activation energy (E) estimated for the FD was 28.35 kJ/mol. The freeze-dried sample was ground and placed under vacuum to reduce the weathering effects. The quality of the stored product was evaluated using the proximate analysis, physicochemical analysis and a sensory evaluation using a hedonic scale. The raw, fresh KF had a moisture content of 85.07% and the final freeze-dried product one of 3%. The carbohydrate, total sugar, protein, fat, total ash, crude fibre and vitamin C contents of the final product increased by 563%, 400%, 355%, 386%, 672%, 106%, and 117% respectively. Of the 66 panelists, the % consumer acceptances for the different attributes were: sweetness (68.18%), sourness (90.91%), saltiness (100%), bitterness (100%), flavour (95.45%), texture (77.27%) and overall acceptability (81.82%). Using conventional freeze-drying (CFD) for blended KF pulp without fortification, with the same RSCSP and the same cubic heater for sublimation, the drying time was found to be 7 h to reach the same final moisture content of 3%.

scholarly journals Effect of Size and Drying Time on the Rehydration and Sensory Properties of Freeze-Dried Snails (Achatina achatina)

2020 ◽  
Vol 2020 ◽  
pp. 1-5
Author(s):  
Matthew A. Achaglinkame ◽  
Eric Owusu-Mensah ◽  
Abena A. Boakye ◽  
Ibok Oduro
Keyword(s):  
Moisture Content ◽  
Nutritional Value ◽  
Freeze Drying ◽  
Consumer Preference ◽  
Sensory Properties ◽  
Temperature Dependent ◽  
Drying Time ◽  
Standard Methods ◽  
Freeze Dried ◽  
Hedonic Scale

Snails, a delicacy in most tropical communities, are highly perishable and seasonal. Employed preservative methods are highly temperature dependent, adversely affecting their nutritional value and sensory properties. This study was aimed at determining the effect of size and drying time on the rehydration and sensory properties of freeze-dried snails. Snails were sized into three categories with average weights: 7.59 g (quarter-sized), 14.41 g (half-sized), and 30.71 g (whole), and freeze-dried for 15, 20, and 25 h. The moisture content and percent rehydration of the dried samples were determined by standard methods and sensory properties assessed by an in-house panel of 30 using a 5-point hedonic scale. The moisture content of the fresh and freeze-dried samples ranged from 65.80 to 75.20% and 3.25 to 10.24%, respectively. Freeze-dried samples had higher percent rehydration (27 to 102%) than the control; smoked snails (21 to 32%). Size had a significant (P<0.05) effect on the rehydration ability of the samples with the half-sized and freeze-dried for 15 h samples having the highest. The freeze-dried samples generally had higher consumer preference than the control in all attributes assessed. The findings show that freeze-drying snails (approximate weight of 14.4 g) for 15 h could be a consumer-preferred alternative preservative method for extending the shelf life of snails.

scholarly journals Determination of shelf-life using accelerated shelf-life testing (ASLT) method and characterization of the flavour components of freeze-dried durian (Durio zibethinus) products

Food Research ◽  
2021 ◽  
Vol 5 (S2) ◽  
pp. 98-106
Author(s):  
S. Darniadi ◽  
D.D. Handoko ◽  
S. Sunarmani ◽  
S. Widowati
Keyword(s):  
Moisture Content ◽  
Shelf Life ◽  
Volatile Compounds ◽  
Freeze Drying ◽  
Solid Phase ◽  
Gas Chromatography Mass Spectrometry ◽  
Life Testing ◽  
Tropical Fruit ◽  
Durio Zibethinus ◽  
Freeze Dried

Durian is a unique tropical fruit that has a strong smell and distinctive taste. It is a seasonal fruit and has a few days shelf-life. Freeze drying is known for preserving foods while maintaining its original shape and provide excellent rehydrated products. This study aimed to determine the shelf-life of freeze-dried (FD) durian products using the accelerated shelf-life testing (ASLT) method and to assess the flavour changes in fresh and freeze-dried durian products. The parameters used to determine shelf-life were moisture content and L* a* b* colour values of FD durian products for 28 days of storage at 30, 40, and 50oC. Flavour analysis using Solid Phase Microextraction (SPME) and Gas Chromatography-Mass Spectrometry (GCMS) was carried out on fresh durian pulp, FD durian for 30 hrs, and FD durian for 36 hrs. The estimation of shelf-life of FD durian products at storage temperatures of 25 and 30oC, respectively, were based on the following parameters: (1) moisture content: 41 and 37 days, (2) L*(brightness): 467 and 311 days, (3) a* (redness): 144 and 171 days, and (4) b*(yellowness): 43 and 46 days. A total of twenty-four volatile compounds contributed to the flavour of fresh durian fruit and five of them had concentrations of more than 10 ppm. The losses percentage of these five volatile compounds were in a range of 78-95% (FD durian for 30 hrs) and a range of 0- 100% (FD durian for 36 hrs). Freeze-drying technique on durian was able to extend shelflife and preserve flavour compounds.

scholarly journals Proximate Analysis of Goat Milk Yogurt Powder Produced by Freeze Drying and Vacuum-Oven Drying and in Comparing with Freeze-Dried Yogurt Powder Prepared with Tualang Honey

2021 ◽  
Vol 12 (1S) ◽  
pp. 101-111
Author(s):  
Siti Norhannani Ahmat Azemi ◽  
Norshafiqah Zainul ◽  
Asmaliza Abd. Ghani ◽  
John Tang Yew Huat
Keyword(s):  
Moisture Content ◽  
Protein Content ◽  
Freeze Drying ◽  
Ph Value ◽  
Goat Milk ◽  
Drying Method ◽  
Vacuum Oven ◽  
Oven Drying ◽  
Freeze Dried ◽  
Tualang Honey

This study was carried out to produce powdered yogurt from goat milk with longer shelf life.Two methods of drying process of yogurt were used which are vacuum-oven drying (VD) and freezedrying (FD). Goat milk yogurt powder prepared with added Tualang honey (TH) was produced by freeze-drying method. In this study, four formulations of yogurt were prepared with the addition of commercial yogurt containingas starter culture.Granulated sugar was added about 8% into the yogurt as control sample. While other three yogurts were prepared with 8% of Tualang honey, 6% of Tualang honey with 2% of sugar and 4% of Tualang honey with 4% of sugar. The proximateanalysis was used in order to identify the proximate composition and pH value of the yogurt. The moisture content of goat milk yogurt, VD goat yogurt powder and FD goat yogurt powder were 79.20%, 8.22% and 9.66% respectively. While, the moisture content for FD goat yogurt powder with addition of 4%, 6% and 8% Tualang honey were 15.12%,15.92% and 13.53% respectively.While the value of ash content for FD goat milk yogurt powder with addition of 4%, 6% and 8% Tualang honey were 0.37%, 0.35% and 0.50% respectively. The total protein content for goat milk yogurt was 4.61% whilein VD goat yogurt powder and FD goat yogurt powder were 15.04% and 15.07% respectively. The value of protein content for FD goat milk yogurt powder with addition of 8% of Tualang honey was 15.38%. The pH value ofgoat milk sample and fresh yogurt goat milk were 6.52 and 3.82. The pH values for fresh yogurt with addition of 4%, 6% and 8% of Tualang honey were 4.64, 4.68 and 4.73.Vacuum-oven drying method and freeze-drying method did not show any significant different in moisture, ash and protein content but showed significant different in pH value.

scholarly journals The Freeze-Drying of Foods—The Characteristic of the Process Course and the Effect of Its Parameters on the Physical Properties of Food Materials

Foods ◽  
2020 ◽  
Vol 9 (10) ◽  
pp. 1488 ◽  
Author(s):  
Dorota Nowak ◽  
Ewa Jakubczyk
Keyword(s):  
Mechanical Properties ◽  
Physical Properties ◽  
Structural Properties ◽  
Freeze Drying ◽  
Process Conditions ◽  
Drying Process ◽  
High Quality ◽  
Freeze Dried ◽  
Secondary Drying ◽  
Selection Of

Freeze-drying, also known as lyophilization, is a process in which water in the form of ice under low pressure is removed from a material by sublimation. This process has found many applications for the production of high quality food and pharmaceuticals. The main steps of the freeze-drying process, such as the freezing of the product and primary and secondary drying, are described in this paper. The problems and mechanisms of each step of the freeze-drying process are also analyzed. The methods necessary for the selection of the primary and secondary end processes are characterized. The review contains a description of the effects of process conditions and the selected physical properties of freeze-dried materials, such as structural properties (shrinkage and density porosity), color, and texture. The study shows that little attention is given to the mechanical properties and texture of freeze-dried materials obtained from different conditions of the lyophilization process.

scholarly journals Optimization of the bioactive compounds content in raspberry during freeze-drying using response surface method

2015 ◽  
Vol 21 (1-1) ◽  
pp. 53-61 ◽  
Author(s):  
Vesna Tumbas-Saponjac ◽  
Gordana Cetkovic ◽  
Sladjana Stajcic ◽  
Jelena Vulic ◽  
Jasna Canadanovic-Brunet ◽  
...  
Keyword(s):  
Response Surface ◽  
Response Surface Method ◽  
Bioactive Compounds ◽  
Freeze Drying ◽  
Optimal Conditions ◽  
Total Anthocyanin ◽  
Factors Affecting ◽  
Surface Method ◽  
Freeze Dried ◽  
Experimental Values

The production of high-quality freeze-dried raspberry was studied by response surface method. Two independent variables, temperature (X1) and time (X2) were determined as the most important factors affecting the final product quality estimated by the responses: total phenol (Y1), total anthocyanin (Y2), vitamin C (Y3) and total bioactive compounds (Y4) content. A two-factor central composite design was used for freeze-drying experiments. The second order polynomial models obtained were found to be significant (p<0.05) for all responses. The statistical analysis of experimental data indicated that only quadratic time variable (X22) had significant (p<0.05) effect on all responses. The optimal conditions for all responses combined were found to be: -31 ?C and 35 h. The experimental values of all responses obtained under optimal conditions were in good agreement with predicted values which enables the use of the proposed mathematical models for optimization of investigated process.

EFFECT OF FREEZE-DRYING ON THE SURFACE PROPERTIES OF SOILS AS MEASURED BY THE HEAT OF IMMERSION

1971 ◽  
Vol 51 (2) ◽  
pp. 229-233 ◽  
Author(s):  
FREDERICK BISAL ◽  
W. L. PELTON
Keyword(s):  
Moisture Content ◽  
Soil Properties ◽  
Surface Properties ◽  
Freeze Drying ◽  
Physical Effect ◽  
Dry Process ◽  
Heat Of Immersion ◽  
Freeze Dried ◽  
Integral Heat

The integral heat of immersion was determined for three textural classes of soils which had been freeze-dried, air-dried, oven-dried, alternately frozen and thawed and then freeze-dried, alternately frozen and thawed and then air-dried, alternately frozen and thawed and then oven-dried, and freeze-dried and then oven-dried. There were highly significant differences in the heat of immersion as a result of texture. The significant differences found in the various drying treatments could only be attributed to moisture content at the time of immersion. The physical effect of the freeze-dry process is transient and it does not alter the inherent soil properties with respect to clod formation.

Development of a Functional Beverage Formulation with High Protein Content, Inulin and Stevia

2011 ◽  
Vol 7 (3) ◽  
Author(s):  
Laura T Rodriguez Furlán ◽  
Antonio Pérez Padilla ◽  
Mercedes Campderros
Keyword(s):  
Freeze Drying ◽  
Protein Supplement ◽  
Protective Agent ◽  
Protein Concentrate ◽  
High Solubility ◽  
Food Ingredient ◽  
High Quality ◽  
High Protein Content ◽  
Bovine Plasma ◽  
Low Carbohydrate

A beverage formulation with proteins of high quality, inulin, Stevia and C vitamin has been prepared. The protein source was bovine plasma which was concentrated and demineralized by a combined membrane methodologies as microfiltration, ultrafiltration and discontinues diafiltration. The use of inulin as protective agent to prevent protein concentrate denaturation during freeze-drying was assayed. The inulin is a versatile food ingredient and it also has health benefits. The powder obtained has improved sensory characteristic and high solubility. Stevia has been added as sweetness, being a low-carbohydrate, low-sugar food alternative. The formulation could be destined as protein supplement with functional properties or for special dietary use.

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