scholarly journals Electrochemical Immunosensor Based on Polythionine/Gold Nanoparticles for the Determination of Aflatoxin B1

Sensors ◽  
2008 ◽  
Vol 8 (12) ◽  
pp. 8262-8274 ◽  
Author(s):  
Joseph Owino ◽  
Omotayo Arotiba ◽  
Nicolette Hendricks ◽  
Everlyne Songa ◽  
Nazeem Jahed ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Aflatoxin B1 ◽  
Electrochemical Immunosensor

scholarly journals An Impedance Based Electrochemical Immunosensor for Aflatoxin B1 Monitoring in Pistachio Matrices

Chemosensors ◽  
2020 ◽  
Vol 8 (4) ◽  
pp. 121
Author(s):  
Michail D. Kaminiaris ◽  
Sophie Mavrikou ◽  
Maria Georgiadou ◽  
Georgia Paivana ◽  
Dimitrios I. Tsitsigiannis ◽  
...  
Keyword(s):  
Aflatoxin B1 ◽  
Limit Of Detection ◽  
Electrochemical Immunosensor ◽  
Aflatoxin M1 ◽  
Biomolecular Recognition ◽  
Effective Prevention ◽  
Upper Limits ◽  
Ethylcarbodiimide Hydrochloride ◽  
Food And Feed

Aflatoxins are highly toxic fungal secondary metabolites that often contaminate food and feed commodities. An electrochemical immunosensor for the determination of aflatoxin B1 (AFB1) was fabricated by immobilizing monoclonal AFB1 antibodies onto a screen-printed gold electrode that was modified with carbo-methyldextran by N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride/N-hydroxysuccinimide cross-linking. An electrochemical interfacial modelling of biomolecular recognition was suggested and reasonably interpreted. Impedance technology was employed for the quantitative determination of AFB1. The limit of detection concentration of AFB1 for standard solutions and spiked pistachio samples was 0.5 ng/mL and 1 ng/mL, respectively. The immunosensor was able to successfully determine AFB1 concentrations in the range of 4.56–50.86 ng/mL in unknown pistachio samples. Comparative chromatographic analysis revealed that AFB1 concentrations that were higher than 345 ng/mL were not within the immunosensor’s upper limits of detection. Selectivity studies against Ochratoxin A and Aflatoxin M1 demonstrated that the proposed AFB1 immunosensor was able to differentiate between these other fungal mycotoxins. The novel electrochemical immunosensor approach has the potential for rapid sample screening in a portable, disposable format, thus contributing to the requirement for effective prevention and the control of aflatoxin B1 in pistachios.

Integrated electrochemical immunosensor with gold nanoparticles for the determination of progesterone

2012 ◽  
Vol 166-167 ◽  
pp. 586-592 ◽  
Author(s):  
Melisa Jimena Monerris ◽  
Fernando Javier Arévalo ◽  
Héctor Fernández ◽  
María Alicia Zon ◽  
Patricia Gabriela Molina
Keyword(s):  
Gold Nanoparticles ◽  
Electrochemical Immunosensor

scholarly journals Horseradish Peroxidase Labelled-Sandwich Electrochemical Sensor Based on Ionic Liquid-Gold Nanoparticles for Lactobacillus brevis

Micromachines ◽  
2021 ◽  
Vol 12 (1) ◽  
pp. 75
Author(s):  
Le Zhao
Keyword(s):  
Gold Nanoparticles ◽  
Ionic Liquid ◽  
Limit Of Detection ◽  
Lactobacillus Brevis ◽  
Electrochemical Immunosensor ◽  
Linear Regression Equation ◽  
Liquid Gold ◽  
Beer Spoilage ◽  
Sensitive Determination

Lactobacillus brevis is the most common bacteria that causes beer spoilage. In this work, a novel electrochemical immunosensor was fabricated for ultra-sensitive determination of L. brevis. Gold nanoparticles (AuNPs) were firstly electro-deposited on the electrode surface for enhancing the electro-conductivity and specific surface area. Ionic liquid was used for improving the immobilization performance of the immunosensor. After optimization, a linear regression equation can be observed between the ∆current and concentration of L. brevis from 104 CFU/mL to 109 CFU/mL. The limit of detection can be estimated to be 103 CFU/mL.

Polydopamine and graphene oxide synergistically modified Prussian blue electrochemical immunosensor for the detection of alpha-fetoprotein with enhanced stability and sensibility

RSC Advances ◽  
2015 ◽  
Vol 5 (48) ◽  
pp. 38176-38182 ◽  
Author(s):  
Ren-zhong Zhang ◽  
Bo-guang Pan ◽  
Hai-ning Wang ◽  
Jian-ming Dan ◽  
Cheng-lin Hong ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Graphene Oxide ◽  
Prussian Blue ◽  
Electrochemical Immunosensor ◽  
Alpha Fetoprotein ◽  
Amperometric Immunosensor ◽  
Highly Sensitive ◽  
Enhanced Stability

In this paper, a simple and highly sensitive amperometric immunosensor for the determination of alpha-fetoprotein based on multi-functional gold nanoparticles–polydopamine-Prussian blue–graphene oxide nanocomposites (Au–PDA-PB–GO) was fabricated.

Sign in / Sign up

Export Citation Format

Share Document