scholarly journals Recent Advances in Quenchbody, a Fluorescent Immunosensor

Sensors ◽  
2021 ◽  
Vol 21 (4) ◽  
pp. 1223
Author(s):  
Jinhua Dong ◽  
Hiroshi Ueda
Keyword(s):  
Fluorescence Intensity ◽  
Fluorescent Dyes ◽  
Antibody Fragment ◽  
Disease Diagnosis ◽  
Disease Biomarkers ◽  
Highly Sensitive ◽  
Physiologically Active Substances ◽  
New Type ◽  
Antigen Antibody ◽  
Active Substances

The detection of viruses, disease biomarkers, physiologically active substances, drugs, and chemicals is of great significance in many areas of our lives. Immunodetection technology is based on the specificity and affinity of antigen–antibody reactions. Compared with other analytical methods such as liquid chromatography coupled with mass spectrometry, which requires a large and expensive instrument, immunodetection has the advantages of simplicity and good selectivity and is thus widely used in disease diagnosis and food/environmental monitoring. Quenchbody (Q-body), a new type of fluorescent immunosensor, is an antibody fragment labeled with fluorescent dyes. When the Q-body binds to its antigen, the fluorescence intensity increases. The detection of antigens by changes in fluorescence intensity is simple, easy to operate, and highly sensitive. This review comprehensively discusses the principle, construction, application, and current progress related to Q-bodies.

scholarly journals A Comprehensive Overview of Novel Hydroxynitrile Lyases

2021 ◽  
Author(s):  
Asha Kumari ◽  
Garima Chauhan ◽  
Meghna Arya ◽  
Monica Sharma
Keyword(s):  
Evolutionary History ◽  
Comprehensive Overview ◽  
Recent Advances ◽  
Wide Range ◽  
Previous Decade ◽  
Physiologically Active Substances ◽  
New Type ◽  
Active Substances

Hydroxynitrile lyases (HNLs) are a heterogeneous family of enzymes that are of particular interest because of their structurally unique categories, a wide range of immobilisation techniques and procedures, and a wide range of sources with varying degrees of enantiopurity and enantioselectivity. Cupin, which contains a new type of HNL from bacteria, the lipocalin superfamily, which has HNLs from millipedes, and the + barrel fold superfamily, which contains HNL from a fern, have all been discovered in recent decades. Their biochemistry has been deciphered, and engineering efforts have been made to boost their productivity, purity, and activity. These remarkable enzymes opened up a new vista in the field of industrial catalysts since they are actively used in the synthesis of crucially important agrochemicals, medicines, physiologically active substances, and chemo-enzymatic follow-up procedures. This review focuses on recent advances, evolutionary history, and recombinant engineering of HNL from the previous decade.

scholarly journals Gold and ZnO-Based Metal-Semiconductor Network for Highly Sensitive Room-Temperature Gas Sensing

Sensors ◽  
2019 ◽  
Vol 19 (18) ◽  
pp. 3815
Author(s):  
Renyun Zhang ◽  
Magnus Hummelgård ◽  
Joel Ljunggren ◽  
Håkan Olin
Keyword(s):  
Solar Cells ◽  
Gas Sensor ◽  
Network Structure ◽  
Room Temperature ◽  
Gas Sensing ◽  
Zno Nanowires ◽  
Gold Particles ◽  
Highly Sensitive ◽  
Semiconductor Electronics ◽  
New Type

Metal-semiconductor junctions and interfaces have been studied for many years due to their importance in applications such as semiconductor electronics and solar cells. However, semiconductor-metal networks are less studied because there is a lack of effective methods to fabricate such structures. Here, we report a novel Au–ZnO-based metal-semiconductor (M-S)n network in which ZnO nanowires were grown horizontally on gold particles and extended to reach the neighboring particles, forming an (M-S)n network. The (M-S)n network was further used as a gas sensor for sensing ethanol and acetone gases. The results show that the (M-S)n network is sensitive to ethanol (28.1 ppm) and acetone (22.3 ppm) gases and has the capacity to recognize the two gases based on differences in the saturation time. This study provides a method for producing a new type of metal-semiconductor network structure and demonstrates its application in gas sensing.

ChemInform Abstract: Progress in the Field of Physiologically Active Substances of Lanosterol Series

ChemInform ◽  
2010 ◽  
Vol 24 (30) ◽  
pp. no-no
Author(s):  
I. G. RESHETOVA ◽  
R. K. TKHAPER ◽  
A. V. KAMERNITSKII
Keyword(s):  
Physiologically Active Substances ◽  
Active Substances

scholarly journals Methodology and Applications of Disease Biomarker Identification in Human Serum

2007 ◽  
Vol 2 ◽  
pp. 117727190700200 ◽  
Author(s):  
Ziad J. Sahab ◽  
Suzan M. Semaan ◽  
Qing-Xiang Amy Sang
Keyword(s):  
Human Serum ◽  
Protein Separation ◽  
High Sensitivity ◽  
Disease Diagnosis ◽  
Plasma Lipoproteins ◽  
Great Promise ◽  
Protein Biomarkers ◽  
Serum Samples ◽  
Disease Biomarkers ◽  
Diagnosis And Prognosis

Biomarkers are biomolecules that serve as indicators of biological and pathological processes, or physiological and pharmacological responses to a drug treatment. Because of the high abundance of albumin and heterogeneity of plasma lipoproteins and glycoproteins, biomarkers are difficult to identify in human serum. Due to the clinical significance the identification of disease biomarkers in serum holds great promise for personalized medicine, especially for disease diagnosis and prognosis. This review summarizes some common and emerging proteomics techniques utilized in the separation of serum samples and identification of disease signatures. The practical application of each protein separation or identification technique is analyzed using specific examples. Biomarkers of cancers of prostate, breast, ovary, and lung in human serum have been reviewed, as well as those of heart disease, arthritis, asthma, and cystic fibrosis. Despite the advancement of technology few biomarkers have been approved by the Food and Drug Administration for disease diagnosis and prognosis due to the complexity of structure and function of protein biomarkers and lack of high sensitivity, specificity, and reproducibility for those putative biomarkers. The combination of different types of technologies and statistical analysis may provide more effective methods to identify and validate new disease biomarkers in blood.

scholarly journals Physical and chemical parameters impact on eosin spectral determinations

2010 ◽  
Vol 29 (1) ◽  
pp. 51
Author(s):  
Liljana Kola
Keyword(s):  
Fluorescence Intensity ◽  
Water Samples ◽  
Measurement Errors ◽  
Fluorescent Dyes ◽  
Water System ◽  
Chemical Properties ◽  
Photochemical Decomposition ◽  
Large Measurement ◽  
Physical And Chemical ◽  
And Chemical Properties

The fluorescence ability of Eosin enables its using as an artificial tracer in the water system studies. The problem deals with the application of Eosin to trace and determine water movements within the karstic system and under ground waters. The fluorescence intensity of fluorescent dyes in water samples depends on their physical and chemical properties, such as pH, temperature, presence of oxidants, etc. Besides that, the UV radiation may induce photochemical decomposition of Eosin which can cause large measurement errors. This paper presents the taken results studying the influence of these factors on Eosin fluorescence intensity using the concentration and synchron scan methods. The method we have elaborated for this purpose made it possible to optimize procedures we use to analyze water samples for the presence of Eosin and measure its content, even in trace levels by the means of the Perkin Elmer LS 55 Luminescence Spectrometer.

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