scholarly journals 3D-Printed Sensors and Actuators in Cell Culture and Tissue Engineering: Framework and Research Challenges

Sensors ◽  
2020 ◽  
Vol 20 (19) ◽  
pp. 5617
Author(s):  
Pablo Pérez ◽  
Juan Alfonso Serrano ◽  
Alberto Olmo
Keyword(s):  
Tissue Engineering ◽  
Cell Culture ◽  
3D Printing ◽  
Regenerative Medicine ◽  
Electrical Impedance Spectroscopy ◽  
Special Focus ◽  
Tissue Formation ◽  
Sensors And Actuators ◽  
Printed Sensors ◽  
3D Printed

Three-dimensional printing technologies have been recently proposed to monitor cell cultures and implement cell bioreactors for different biological applications. In tissue engineering, the control of tissue formation is crucial to form tissue constructs of clinical relevance, and 3D printing technologies can also play an important role for this purpose. In this work, we study 3D-printed sensors that have been recently used in cell culture and tissue engineering applications in biological laboratories, with a special focus on the technique of electrical impedance spectroscopy. Furthermore, we study new 3D-printed actuators used for the stimulation of stem cells cultures, which is of high importance in the process of tissue formation and regenerative medicine. Key challenges and open issues, such as the use of 3D printing techniques in implantable devices for regenerative medicine, are also discussed.

scholarly journals Recent Progress in 3D Printed Mold-Based Sensors

Sensors ◽  
2020 ◽  
Vol 20 (3) ◽  
pp. 703 ◽  
Author(s):  
Shan He ◽  
Shilun Feng ◽  
Anindya Nag ◽  
Nasrin Afsarimanesh ◽  
Tao Han ◽  
...  
Keyword(s):  
3D Printing ◽  
Recent Progress ◽  
Raw Materials ◽  
Electronic Waste ◽  
Market Survey ◽  
Sensors And Actuators ◽  
Significant Research ◽  
Printed Sensors ◽  
3D Printed ◽  
Different Parts

The paper presents a review of some of the significant research done on 3D printed mold-based sensors performed in recent times. The utilization of the master molds to fabricate the different parts of the sensing prototypes have been followed for quite some time due to certain distinct advantages. Some of them are easy template preparation, easy customization of the developed products, quick fabrication, and minimized electronic waste. The paper explains the different kinds of sensors and actuators that have been developed using this technique, based on their varied structural dimensions, processed raw materials, designing, and product testing. These differences in the attributes were based on their individualistic application. Furthermore, some of the challenges related to the existing sensors and their possible respective solutions have also been mentioned in the paper. Finally, a market survey has been provided, stating the estimated increase in the annual growth of 3D printed sensors. It also states the type of 3D printing that has been preferred over the years, along with the range of sensors, and their related applications.

Biomimetic Design of 3D Printed Tissue-engineered bone Constructs

2020 ◽  
Vol 16 ◽  
Author(s):  
Wei Liu ◽  
Shifeng Liu ◽  
Yunzhe Li ◽  
Peng Zhou ◽  
Qian ma
Keyword(s):  
Tissue Engineering ◽  
3D Printing ◽  
Advanced Materials ◽  
Bionic Design ◽  
Material Interfaces ◽  
Precise Control ◽  
Cell Printing ◽  
Biomimetic Scaffolds ◽  
3D Printed

Abstract:: Surgery to repair damaged tissue, which is caused by disease or trauma, is being carried out all the time, and a desirable treatment is compelling need to regenerate damaged tissues to further improve the quality of human health. Therefore, more and more research focus on exploring the most suitable bionic design to enrich available treatment methods. 3D-printing, as an advanced materials processing approach, holds promising potential to create prototypes with complex constructs that could reproduce primitive tissues and organs as much as possible or provide appropriate cell-material interfaces. In a sense, 3D printing promises to bridge between tissue engineering and bionic design, which can provide an unprecedented personalized recapitulation with biomimetic function under the precise control of the composition and spatial distribution of cells and biomaterials. This article describes recent progress in 3D bionic design and the potential application prospect of 3D printing regenerative medicine including 3D printing biomimetic scaffolds and 3D cell printing in tissue engineering.

scholarly journals Three-Dimensional Printing of Hydroxyapatite Composites for Biomedical Application

Crystals ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 353
Author(s):  
Yanting Han ◽  
Qianqian Wei ◽  
Pengbo Chang ◽  
Kehui Hu ◽  
Oseweuba Valentine Okoro ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
3D Printing ◽  
Biomedical Application ◽  
Three Dimensional ◽  
Antibacterial Properties ◽  
Natural Polymers ◽  
Three Dimensional Printing ◽  
3D Printed ◽  
Dental Applications ◽  
Hard Tissue Engineering

Hydroxyapatite (HA) and HA-based nanocomposites have been recognized as ideal biomaterials in hard tissue engineering because of their compositional similarity to bioapatite. However, the traditional HA-based nanocomposites fabrication techniques still limit the utilization of HA in bone, cartilage, dental, applications, and other fields. In recent years, three-dimensional (3D) printing has been shown to provide a fast, precise, controllable, and scalable fabrication approach for the synthesis of HA-based scaffolds. This review therefore explores available 3D printing technologies for the preparation of porous HA-based nanocomposites. In the present review, different 3D printed HA-based scaffolds composited with natural polymers and/or synthetic polymers are discussed. Furthermore, the desired properties of HA-based composites via 3D printing such as porosity, mechanical properties, biodegradability, and antibacterial properties are extensively explored. Lastly, the applications and the next generation of HA-based nanocomposites for tissue engineering are discussed.

scholarly journals Three-dimensionally printed polycaprolactone/multicomponent bioactive glass scaffolds for potential application in bone tissue engineering

2020 ◽  
Vol 6 (1) ◽  
pp. 57-69
Author(s):  
Amirhosein Fathi ◽  
Farzad Kermani ◽  
Aliasghar Behnamghader ◽  
Sara Banijamali ◽  
Masoud Mozafari ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
3D Printing ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Three Dimensional ◽  
Layer By Layer ◽  
Composite Scaffolds ◽  
3D Printed ◽  
Co Doped

AbstractOver the last years, three-dimensional (3D) printing has been successfully applied to produce suitable substitutes for treating bone defects. In this work, 3D printed composite scaffolds of polycaprolactone (PCL) and strontium (Sr)- and cobalt (Co)-doped multi-component melt-derived bioactive glasses (BGs) were prepared for bone tissue engineering strategies. For this purpose, 30% of as-prepared BG particles (size <38 μm) were incorporated into PCL, and then the obtained composite mix was introduced into a 3D printing machine to fabricate layer-by-layer porous structures with the size of 12 × 12 × 2 mm3.The scaffolds were fully characterized through a series of physico-chemical and biological assays. Adding the BGs to PCL led to an improvement in the compressive strength of the fabricated scaffolds and increased their hydrophilicity. Furthermore, the PCL/BG scaffolds showed apatite-forming ability (i.e., bioactivity behavior) after being immersed in simulated body fluid (SBF). The in vitro cellular examinations revealed the cytocompatibility of the scaffolds and confirmed them as suitable substrates for the adhesion and proliferation of MG-63 osteosarcoma cells. In conclusion, 3D printed composite scaffolds made of PCL and Sr- and Co-doped BGs might be potentially-beneficial bone replacements, and the achieved results motivate further research on these materials.

Tissue engineering and 3D printing of bioartificial pancreas for regenerative medicine in diabetes

2021 ◽  
Author(s):  
Andreas Alvin Purnomo Soetedjo ◽  
Jia Min Lee ◽  
Hwee Hui Lau ◽  
Guo Liang Goh ◽  
Jia An ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
3D Printing ◽  
Regenerative Medicine ◽  
Bioartificial Pancreas

Electrical impedance spectroscopy – a potential method for the study and monitoring of a bone critical-size defect healing process treated with bone tissue engineering and regenerative medicine approaches

2016 ◽  
Vol 4 (16) ◽  
pp. 2757-2767 ◽  
Author(s):  
Evgeny Kozhevnikov ◽  
Xiaolu Hou ◽  
Shupei Qiao ◽  
Yufang Zhao ◽  
Chunfeng Li ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Regenerative Medicine ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Critical Size ◽  
Healing Process ◽  
Potential Method ◽  
Electrical Impedance Spectroscopy ◽  
Critical Size Defect ◽  
Critical Size Defects

The development of strategies of bone tissue engineering and regenerative medicine has been drawing considerable attention to treat bone critical-size defects (CSDs).

Control of cell growth on 3D-printed cell culture platforms for tissue engineering

2017 ◽  
Vol 105 (12) ◽  
pp. 3281-3292 ◽  
Author(s):  
Zhikai Tan ◽  
Tong Liu ◽  
Juchang Zhong ◽  
Yikun Yang ◽  
Weihong Tan
Keyword(s):  
Tissue Engineering ◽  
Cell Culture ◽  
Cell Growth ◽  
3D Printed

scholarly journals 3D Printed Chromophoric Sensors

Chemosensors ◽  
2021 ◽  
Vol 9 (11) ◽  
pp. 317
Author(s):  
Zachary Brounstein ◽  
Jarrod Ronquillo ◽  
Andrea Labouriau
Keyword(s):  
Thermal Stability ◽  
3D Printing ◽  
Material Properties ◽  
Chemical Structure ◽  
Elevated Temperatures ◽  
Advanced Manufacturing ◽  
Color Changes ◽  
Printed Sensors ◽  
3D Printed ◽  
Manufacturing Techniques

Eight chromophoric indicators are incorporated into Sylgard 184 to develop sensors that are fabricated either by traditional methods such as casting or by more advanced manufacturing techniques such as 3D printing. The sensors exhibit specific color changes when exposed to acidic species, basic species, or elevated temperatures. Additionally, material properties are investigated to assess the chemical structure, Shore A Hardness, and thermal stability. Comparisons between the casted and 3D printed sensors show that the sensing devices fabricated with the advanced manufacturing technique are more efficient because the color changes are more easily detected.

scholarly journals Additive Manufacturing of Biopolymers for Tissue Engineering and Regenerative Medicine: An Overview, Potential Applications, Advancements, and Trends

2021 ◽  
Vol 2021 ◽  
pp. 1-20 ◽  
Author(s):  
Dhinakaran Veeman ◽  
M. Swapna Sai ◽  
P. Sureshkumar ◽  
T. Jagadeesha ◽  
L. Natrayan ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Additive Manufacturing ◽  
3D Printing ◽  
Regenerative Medicine ◽  
Tissue Regeneration ◽  
Three Dimensional ◽  
Porous Scaffolds ◽  
Wide Range ◽  
Potential Applications ◽  
Pharmaceutical Industries

As a technique of producing fabric engineering scaffolds, three-dimensional (3D) printing has tremendous possibilities. 3D printing applications are restricted to a wide range of biomaterials in the field of regenerative medicine and tissue engineering. Due to their biocompatibility, bioactiveness, and biodegradability, biopolymers such as collagen, alginate, silk fibroin, chitosan, alginate, cellulose, and starch are used in a variety of fields, including the food, biomedical, regeneration, agriculture, packaging, and pharmaceutical industries. The benefits of producing 3D-printed scaffolds are many, including the capacity to produce complicated geometries, porosity, and multicell coculture and to take growth factors into account. In particular, the additional production of biopolymers offers new options to produce 3D structures and materials with specialised patterns and properties. In the realm of tissue engineering and regenerative medicine (TERM), important progress has been accomplished; now, several state-of-the-art techniques are used to produce porous scaffolds for organ or tissue regeneration to be suited for tissue technology. Natural biopolymeric materials are often better suited for designing and manufacturing healing equipment than temporary implants and tissue regeneration materials owing to its appropriate properties and biocompatibility. The review focuses on the additive manufacturing of biopolymers with significant changes, advancements, trends, and developments in regenerative medicine and tissue engineering with potential applications.

scholarly journals Methods of Modification of Mesenchymal Stem Cells and Conditions of Their Culturing for Hyaline Cartilage Tissue Engineering

Biomedicines ◽  
2021 ◽  
Vol 9 (11) ◽  
pp. 1666
Author(s):  
Maria V. Shestovskaya ◽  
Svetlana A. Bozhkova ◽  
Julia V. Sopova ◽  
Mikhail G. Khotin ◽  
Mikhail S. Bozhokin
Keyword(s):  
Tissue Engineering ◽  
Cell Culture ◽  
Clinical Practice ◽  
Regenerative Medicine ◽  
Hyaline Cartilage ◽  
Cartilage Tissue Engineering ◽  
Matrix Proteins ◽  
Cartilage Tissue ◽  
Biodegradable Scaffolds ◽  
Cartilage Extracellular Matrix

The use of mesenchymal stromal cells (MSCs) for tissue engineering of hyaline cartilage is a topical area of regenerative medicine that has already entered clinical practice. The key stage of this procedure is to create conditions for chondrogenic differentiation of MSCs, increase the synthesis of hyaline cartilage extracellular matrix proteins by these cells and activate their proliferation. The first such works consisted in the indirect modification of cells, namely, in changing the conditions in which they are located, including microfracturing of the subchondral bone and the use of 3D biodegradable scaffolds. The most effective methods for modifying the cell culture of MSCs are protein and physical, which have already been partially introduced into clinical practice. Genetic methods for modifying MSCs, despite their effectiveness, have significant limitations. Techniques have not yet been developed that allow studying the effectiveness of their application even in limited groups of patients. The use of MSC modification methods allows precise regulation of cell culture proliferation, and in combination with the use of a 3D biodegradable scaffold, it allows obtaining a hyaline-like regenerate in the damaged area. This review is devoted to the consideration and comparison of various methods used to modify the cell culture of MSCs for their use in regenerative medicine of cartilage tissue.

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