scholarly journals Functionalized Fluorescent Silica Nanoparticles for Bioimaging of Cancer Cells

Sensors ◽  
2020 ◽  
Vol 20 (19) ◽  
pp. 5590
Author(s):  
Ruth Prieto-Montero ◽  
Alberto Katsumiti ◽  
Miren Pilare Cajaraville ◽  
Iñigo López-Arbeloa ◽  
Virginia Martínez-Martínez
Keyword(s):  
Silica Nanoparticles ◽  
Cancer Cells ◽  
Hela Cells ◽  
Sol Gel ◽  
Synthesis Method ◽  
In Vitro Assays ◽  
Quantum Yields ◽  
Fluorescent Nanoparticles ◽  
Fluorescent Silica Nanoparticles

Functionalized fluorescent silica nanoparticles were designed and synthesized to selectively target cancer cells for bioimaging analysis. The synthesis method and characterization of functionalized fluorescent silica nanoparticles (50–60 nm), as well as internalization and subcellular localization in HeLa cells is reported here. The dye, rhodamine 101 (R101) was physically embedded during the sol–gel synthesis. The dye loading was optimized by varying the synthesis conditions (temperature and dye concentration added to the gel) and by the use of different organotriethoxysilanes as a second silica precursor. Additionally, R101, was also covalently bound to the functionalized external surface of the silica nanoparticles. The quantum yields of the dye-doped silica nanoparticles range from 0.25 to 0.50 and demonstrated an enhanced brightness of 230–260 fold respect to the free dye in solution. The shell of the nanoparticles was further decorated with PEG of 2000 Da and folic acid (FA) to ensure good stability in water and to enhance selectivity to cancer cells, respectively. In vitro assays with HeLa cells showed that fluorescent nanoparticles were internalized by cells accumulating exclusively into lysosomes. Quantitative analysis showed a significantly higher accumulation of FA functionalized fluorescent silica nanoparticles compared to nanoparticles without FA, proving that the former may represent good candidates for targeting cancer cells.

scholarly journals The Effect of Rhodamine B on The Properties of Fluorescent Nanoparticles Derived from Geothermal Silica

2020 ◽  
Vol 9 (3) ◽  
pp. 171-176
Author(s):  
Yovilianda Maulitiva Untoro ◽  
◽  
Diaz Ayu Widyasari ◽  
Edi Supriadi ◽  
Siti Nurul Aisyiyah Jenie ◽  
...  
Keyword(s):  
Silica Nanoparticles ◽  
Rhodamine B ◽  
Sol Gel ◽  
Specific Area ◽  
Ammonium Bromide ◽  
Fluorescent Nanoparticles ◽  
Fluorescent Intensity ◽  
Fluorescent Silica Nanoparticles ◽  
Bet Analysis ◽  
Ft Ir

Rhodamine B can be used as a fluorophore to produce fluorescent silica nanoparticles derived from geothermal sludge. The purpose of this research is to synthesize fluorescent silica nanoparticles (FSNP) modified with rhodamine B and cetyl trimethyl ammonium bromide (CTAB) using sol-gel method. Geothermal waste was used as a precursor and added with NaOH at 900C to generate sodium silicate. Rhodamine B, as the fluorescent dye were added with concentration variations ranging from 0.156 mg/g to 10 mg/g.CTAB was used as template and HCl 2N was applied as gelling catalyst with aging time of 18 hours. Characterization of FSNP was measured using spectrofluorometer to identify the fluorescent intensity, fourier transform infrared (FT-IR) to determine the functional group of FSNP, Brauner-Emmett-Teller (BET) adsorption to calculate the specific area of the particles, X-ray diffraction (XRD) to analyze the crystallographic phases, and transmission electron microscopy (TEM) to analyze the surface morphology of the FSNP. FT-IR and fluorescent intensity results showed that FSNP with 2.5 mg/g of rhodamine B had the optimum characteristics. The FSNP was in amorphous phase with uniform pore distribution. BET analysis showed that the specific surface of the FSNP was 190.22 m2/g.

Differently sized drug-loaded mesoporous silica nanoparticles elicit differential gene expression in MCF-7 cancer cells

Nanomedicine ◽  
2021 ◽  
Author(s):  
Baranya Murugan ◽  
Uma Maheswari Krishnan
Keyword(s):  
Gene Expression ◽  
Mesoporous Silica ◽  
Silica Nanoparticles ◽  
Cancer Cells ◽  
Mesoporous Silica Nanoparticles ◽  
Sol Gel ◽  
Anticancer Efficacy ◽  
Mcf 7

Aim: This study investigates the effects of different sized unmodified and chemo-responsive mesoporous silica nanocarriers on MCF-7 cancer cells. Materials & methods: Unmodified and thiol-functionalized large and small-sized mesoporous MCM-41 silica nanoparticles prepared using templated sol-gel process were characterized for their physicochemical properties and in vitro and in vivo anticancer efficacy. Microarray analysis was carried out to assess their differential effect on gene expression. Results: Thiol-functionalized nanoparticles displayed chemo responsive release and greater cytotoxicity to cancer cells when compared with unmodified carriers. Microarray studies showed distinct differences in genes differentially regulated by sMCM-41and lMCM-41 carriers when compared with the free drug. Conclusion: The small chemo-responsive carrier was more effective in suppressing oncogenes and genes involved in proliferation, invasion and survival while the large carrier mainly altered membrane-associated pathways.

scholarly journals Highly Efficient T2 Cobalt Ferrite Nanoparticles Vectorized for Internalization in Cancer Cells

2021 ◽  
Vol 14 (2) ◽  
pp. 124
Author(s):  
Eva Mazarío ◽  
Magdalena Cañete ◽  
Fernando Herranz ◽  
Jorge Sánchez-Marcos ◽  
Jesús M. de la Fuente ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Hela Cells ◽  
Cobalt Ferrite ◽  
Colloidal Suspension ◽  
Aqueous Media ◽  
Synthesis Method ◽  
Ferrite Nanoparticles ◽  
Cobalt Ferrite Nanoparticles ◽  
Vitro Analysis

Uniform cobalt ferrite nanoparticles have been synthesized using an electrochemical synthesis method in aqueous media. Their colloidal, magnetic, and relaxometric properties have been analyzed. The novelty of this synthesis relies on the use of iron and cobalt foils as precursors, which assures the reproducibility of the iron and cobalt ratio in the structure. A stable and biocompatible targeting conjugate nanoparticle-folic acid (NP-FA) was developed that was capable of targeting FA receptor positivity in HeLa (human cervical cancer) cancer cells. The biocompatibility of NP-FA was assessed in vitro in HeLa cells using the MTT assay, and morphological analysis of the cytoskeleton was performed. A high level of NP-FA binding to HeLa cells was confirmed through qualitative in vitro targeting studies. A value of 479 Fe+Co mM−1s−1 of transverse relaxivity (r2) was obtained in colloidal suspension. In addition, in vitro analysis in HeLa cells also showed an important effect in negative T2 contrast. Therefore, the results show that NP-FA can be a potential biomaterial for use in bio medical trials, especially as a contrast agent in magnetic resonance imaging (MRI).

scholarly journals Targeted delivery of mesoporous silica nanoparticles loaded monastrol into cancer cells: an in vitro study

2017 ◽  
Vol 7 (8) ◽  
pp. 549-555 ◽  
Author(s):  
Huzaifa Hanif ◽  
Samina Nazir ◽  
Kehkashan Mazhar ◽  
Muhammad Waseem ◽  
Shazia Bano ◽  
...  
Keyword(s):  
Mesoporous Silica ◽  
Silica Nanoparticles ◽  
Cancer Cells ◽  
Targeted Delivery ◽  
Mesoporous Silica Nanoparticles ◽  
In Vitro Study ◽  
Vitro Study

scholarly journals Large Pore Mesoporous Silica and Organosilica Nanoparticles for Pepstatin A Delivery in Breast Cancer Cells

Molecules ◽  
2019 ◽  
Vol 24 (2) ◽  
pp. 332 ◽  
Author(s):  
Saher Rahmani ◽  
Jelena Budimir ◽  
Mylene Sejalon ◽  
Morgane Daurat ◽  
Dina Aggad ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Mesoporous Silica ◽  
Silica Nanoparticles ◽  
Cancer Cells ◽  
Cathepsin D ◽  
Breast Cancer Cells ◽  
Potent Inhibitor ◽  
Mesoporous Silica Nanoparticles ◽  
Sol Gel ◽  
Pepstatin A

(1) Background: Nanomedicine has recently emerged as a new area of research, particularly to fight cancer. In this field, we were interested in the vectorization of pepstatin A, a peptide which does not cross cell membranes, but which is a potent inhibitor of cathepsin D, an aspartic protease particularly overexpressed in breast cancer. (2) Methods: We studied two kinds of nanoparticles. For pepstatin A delivery, mesoporous silica nanoparticles with large pores (LPMSNs) and hollow organosilica nanoparticles (HOSNPs) obtained through the sol–gel procedure were used. The nanoparticles were loaded with pepstatin A, and then the nanoparticles were incubated with cancer cells. (3) Results: LPMSNs were monodisperse with 100 nm diameter. HOSNPs were more polydisperse with diameters below 100 nm. Good loading capacities were obtained for both types of nanoparticles. The nanoparticles were endocytosed in cancer cells, and HOSNPs led to the best results for cancer cell killing. (4) Conclusions: Mesoporous silica-based nanoparticles with large pores or cavities are promising for nanomedicine applications with peptides.

scholarly journals Silica-Coated Europium-Doped Gadolinium Oxide Nanorods for Dual-Modal Imaging of Cancer Cells

NANO ◽  
2017 ◽  
Vol 12 (06) ◽  
pp. 1750073 ◽  
Author(s):  
T. Gayathri ◽  
R. Arun Kumar ◽  
B. S. Panigrahi ◽  
B. Devanand
Keyword(s):  
Cancer Cells ◽  
Synthesis Method ◽  
Life Time ◽  
Gadolinium Oxide ◽  
In Vitro Cytotoxicity ◽  
Precipitation Method ◽  
Osteosarcoma Cell ◽  
Oxide Nanorods ◽  
Prepared Nanorods

Dual-modal imaging of cancer cells is possible with the silica-coated europium-doped gadolinium oxide nanorods due to their magnetic and luminescent properties. In the synthesized nanorods, europium ions serve as ‘luminescent centers’ facilitating optical imaging and gadolinium oxide acts as the contrast agent for magnetic resonance imaging (MRI). This article reports the synthesis method of the europium-doped gadolinium oxide (Eu:Gd2O[Formula: see text] nanorods by the co-precipitation method. The prepared nanorods are further coated with silica to improve its biocompatibility. From the x-ray diffraction (XRD) data, the crystallinity was found to decrease due to the amorphous nature of the silica. Transmission electron microscopy (TEM) studies show that Eu:Gd2O3 nanorods with a length of [Formula: see text][Formula: see text]600[Formula: see text]nm and diameter of [Formula: see text][Formula: see text]40[Formula: see text]nm were formed. Silica was coated uniformly with the thickness of [Formula: see text][Formula: see text]15[Formula: see text]nm. Fourier transform infrared spectroscopy (FTIR) confirms the presence of silica in the prepared nanorods. Emission at 611[Formula: see text]nm due the presence of Eu[Formula: see text] ions was observed. The life time of uncoated and silica-coated nanorods was calculated to be 1.1[Formula: see text]ms and 0.9[Formula: see text]ms, respectively. In vitro cytotoxicity of the synthesized nanorods in MG63 (human osteosarcoma cell line) was assessed by MTT assay. In vitro MRI studies reveal that the prepared nanorods can be used for T1 contrast enhancement.

scholarly journals Low dose amiodarone reduces tumor growth and angiogenesis

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Eliana Steinberg ◽  
Arnon Fluksman ◽  
Chalom Zemmour ◽  
Katerina Tischenko ◽  
Adi Karsch-Bluman ◽  
...  
Keyword(s):  
Side Effects ◽  
Cancer Cells ◽  
Low Dose ◽  
Tumor Model ◽  
In Vitro Assays ◽  
Potential Candidate ◽  
Key Factor ◽  
Angiogenic Effect

Abstract Amiodarone is an anti-arrhythmic drug that was approved by the US Food and Drug Administration (FDA) in 1985. Pre-clinical studies suggest that Amiodarone induces cytotoxicity in several types of cancer cells, thus making it a potential candidate for use as an anti-cancer treatment. However, it is also known to cause a variety of severe side effects. We hypothesized that in addition to the cytotoxic effects observed in cancer cells Amiodarone also has an indirect effect on angiogensis, a key factor in the tumor microenvironment. In this study, we examined Amiodarone's effects on a murine tumor model comprised of U-87 MG glioblastoma multiforme (GBM) cells, known to form highly vascularized tumors. We performed several in vitro assays using tumor and endothelial cells, along with in vivo assays utilizing three murine models. Low dose Amiodarone markedly reduced the size of GBM xenograft tumors and displayed a strong anti-angiogenic effect, suggesting dual cancer fighting properties. Our findings lay the ground for further research of Amiodarone as a possible clinical agent that, used in safe doses, maintains its dual properties while averting the drug’s harmful side effects.

scholarly journals Ultrabright fluorescent silica nanoparticles for in vivo targeting of xenografted human tumors and cancer cells in zebrafish

Nanoscale ◽  
2019 ◽  
Vol 11 (46) ◽  
pp. 22316-22327 ◽  
Author(s):  
Saquib Ahmed M. A. Peerzade ◽  
Xiaodan Qin ◽  
Fabrice J. F. Laroche ◽  
Shajesh Palantavida ◽  
Maxim Dokukin ◽  
...  
Keyword(s):  
Silica Nanoparticles ◽  
Cancer Cells ◽  
Human Tumors ◽  
Fluorescent Silica Nanoparticles

New ultrabright fluorescent silica nanoparticles for targeting cancers in vivo are presented.

3D-microdevice for the in vivo capture of cancer-associated circulating cells.

2017 ◽  
Vol 35 (6_suppl) ◽  
pp. e579-e579
Author(s):  
Hélène Cayron ◽  
Alejandro Kayum Jiménez Zenteno ◽  
Aurore Esteve ◽  
Sylvain Sanson ◽  
Christophe Vieu ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Expression Profiles ◽  
Antigen Expression ◽  
Sampling Bias ◽  
Additional Treatment ◽  
In Vitro Assays ◽  
Major Drawback ◽  
Direct Laser

e579 Background: Circulating tumor cells (CTCs) are cancer cells that have detached from a tumor and have entered into the blood circulation at a very low concentration (D. Shook, Mech. Dev., Nov 2003). CTCs have a strong prognostic value, as their number has been correlated to overall survival in different metastatic cancers (J. S. de Bono, Clin. Cancer Res., Oct 2008). Considering the rareness of CTCs in blood, capturing them in vitro is very challenging. CTCs being mainly larger and less deformable than most of blood cells, ISET was the first system exploiting their physical traits using a filtration membrane to enrich 10mL blood samples (G. Vona, Am. J. Pathol., Jan 2000). However, placing the trapping system directly within the bloodstream would increase the amount of blood screened and ensure no sampling bias. To our knowledge, the only system developed for in vivo capture of CTCs relies on an immunologic detection targeting CTCs with specific epithelial-cell adhesion molecules (N. Saucedo-Zeni, Int. J. Oncol., Oct 2012). The major drawback of this technique is the selection bias induced, given the strong heterogeneity of antigen expression profiles in CTC population as confirmed by several studies. Methods: Our device combines the advantages of in vivo capture and physical trapping of CTCs. A polymeric 3D net-like microdevice is fabricated using a Direct Laser Writing technique (Nanoscribe) and integrated onto a Nitinol guidewire to be introduced into the basilic vein through a routine 20G catheter. To optimize the design, we conducted simulation studies and in vitro assays using a fluidic platform reproducing in vivo conditions. Results: We succeeded in capturing PC3 human prostate cancer cells from 20 mL healthy donor blood spiked with 1,000 PC3 cells in 2 minutes, demonstrating the capability to capture CTCs in conditions close to those found in vivo, in terms of pressure and flow rate and without any additional treatment or dilution of the blood. Conclusions: This device could facilitate treatment personalization and follow-up. Its versatility should render it transposable to the capture of single or clustered CTCs, derived from all types of cancer and, by extension, to other circulating cellular and molecular biomarkers.

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