scholarly journals Fully Automated Lab-On-A-Disc Platform for Loop-Mediated Isothermal Amplification Using Micro-Carbon-Activated Cell Lysis

Sensors ◽  
2020 ◽  
Vol 20 (17) ◽  
pp. 4746
Author(s):  
Moo-Jung Seo ◽  
Jae-Chern Yoo
Keyword(s):  
Deoxyribonucleic Acid ◽  
Infrared Camera ◽  
Dna Amplification ◽  
Heating System ◽  
Cell Lysis ◽  
Heating Method ◽  
Standard Procedures ◽  
Spin Column ◽  
Full Automation ◽  
Real Temperature

Fast and fully automated deoxyribonucleic acid (DNA) amplification methods are of interest in the research on lab-on-a-disc (LOD) platforms because of their full compatibility with the spin-column mechanism using centrifugal force. However, the standard procedures followed in DNA amplification require accurate noncontact temperature control as well as cell lysis at a low temperature to prevent damage to the LOD platform. This requirement makes it challenging to achieve full automation of DNA amplification on an LOD. In this paper, a fully automated LOD capable of performing cell lysis and amplification on a single compact disc of DNA samples is proposed. The proposed system uses micro-carbon to heat DNA samples without damaging the LOD as well as a noncontact heating system and an infrared camera sensor to remotely measure the real temperature of the amplification chamber. Compared with conventional DNA amplification systems, the proposed system has the advantage of full automation of the LOD platform. Experimental results demonstrated that the proposed system offers a stable heating method for DNA amplification and cell lysis.

scholarly journals Thymineless Death in Bacillus subtilis: Correlation Between Cell Lysis and Deoxyribonucleic Acid Breakdown

1974 ◽  
Vol 119 (1) ◽  
pp. 36-43 ◽  
Author(s):  
E. Ephrati-Elizur ◽  
D. Yosuv ◽  
E. Shmueli ◽  
A. Horowitz
Keyword(s):  
Bacillus Subtilis ◽  
Deoxyribonucleic Acid ◽  
Cell Lysis ◽  
Thymineless Death

scholarly journals Karakterisasi Prototipe Heater Element System pada Untai Uji RCCS-RDNK menggunakan Kamera Infra Merah

2020 ◽  
Vol 8 (2) ◽  
pp. 313
Author(s):  
DEDY HARYANTO ◽  
GIARNO GIARNO ◽  
JOKO PRASETIO WITOKO ◽  
GREGORIUS BAMBANG HERU KUSNUGROHO ◽  
RAHAYU KUSUMASTUTI ◽  
...  
Keyword(s):  
Cooling System ◽  
Infrared Camera ◽  
Electric Heating ◽  
Heating System ◽  
Vertical Position ◽  
Horizontal Position ◽  
Heater Element ◽  
Element System ◽  
Observation Method ◽  
Cavity Cooling

ABSTRAKReactor Cavity Cooling System (RCCS) adalah salah satu sistem keselamatan pada Reaktor Daya Non Komersial (RDNK). Untuk mensimulasikan keadaaan tersebut, dibuat prototype Heater Element System (HES) yang merupakan sistem pemanas listrik dan berfungsi untuk memberikan kalor pada simulator dinding RPV (wall) seperti pada acuan RPV tipe HTGR. Tujuan penelitian adalah untuk memperoleh karakteristik temperatur selama pemanasan prototype HES hingga mencapai temperature 400°C berdasarkan posisi vertikal dan horizontal HES. Metode pengamatan dilakukan dengan menggunakan kamera infra merah NEC tipe TH9100ML sebagai alat ukur temperatur dan alat visualisasi ditribusi temperatur. Hasil pengamatan menunjukkan, bahwa kehilangan kalor pada prototipe HES pada posisi vertikal lebih kecil dibandingankan pada posisi horizontal hal ini disebabkan karena luas permukaan pada posisi horizontal sebesar 7,260 cm2 lebih memungkinkan untuk kehilangan kalor lebih besar. Posisi vertikal temperatur yang dicapai pada wall prototipe HES lebih tinggi dibandingkan pada posisi horizontal.Kata Kunci: RCCS, Heater Element System, kamera infra merah, temperatur ABSTRACTReactor Cavity Cooling System (RCCS) is one of the safety systems in Non-Commercial Power Reactors (RDNK). To simulate this situation, a prototype Heater Element System (HES) was made using electric heating system as a heat sources and it serves to provide radiation heat to the RPV wall simulator as the reference of the RPV of HTGR type. The purpose of this study was to obtain the temperature characteristics during the heating of the HES prototype to reach temperatures of 400°C base on HES position, horizontal and vertical positions. The observation method was carried out using a TH9100ML infrared camera NEC type as a temperature measurement and a temperature distribution base on visualization. The observations show that the heat loss in the HES prototype in the vertical position is smaller than in the horizontal position because this is because the surface area in the horizontal position 7.260 cm2 is more likely to lose more heat. The vertical position the temperature achieved on the HES prototype wall is higher than in the horizontal position.Keywords: RCCS, Heater Element System, infrared camera, temperature

scholarly journals Research on multi-stage heating method of high-speed railway emergency traction battery system based on flat heat pipe

2020 ◽  
pp. 172-172
Author(s):  
Xudong Sun ◽  
Xiaoming Xu ◽  
Wei Tang ◽  
Huifen Jin ◽  
Hua Ding
Keyword(s):  
High Speed ◽  
Simulation Analysis ◽  
Heating System ◽  
Heating Time ◽  
Transfer Model ◽  
Generation Model ◽  
Battery System ◽  
High Speed Railway ◽  
Heating Method ◽  
Multi Stage

High-speed rail vehicles need to start the emergency traction battery system in an emergency. In order to ensure that the emergency traction battery system can be quickly started in cold conditions and ensure that the high-speed railway has sufficient mileage, this paper proposes a multi-stage heating method based on flat heat pipes. The thermodynamic parameters of the power battery are obtained by simulation analysis and experimental verification. According to the thermodynamic model, the heat generation model and coupled heat transfer model of the battery are established. Different heating powers are assigned to different heating points and the total heating power is kept constant. Through the analysis results, the multi-stage heating device of the emergency traction battery system can effectively reduce the heating time under cold conditions and the temperature difference between the battery system modules. This paper can provide reference to the design of the battery pack and the matching of the heating system.

Anti-Freezing through Drainage Ensured by Heating with Double Thermal Resources for Construction Joints of Tunnels in Cold Area

2010 ◽  
Vol 168-170 ◽  
pp. 2648-2653
Author(s):  
Yi Min Wu
Keyword(s):  
Electrical Energy ◽  
Longitudinal Direction ◽  
Heating System ◽  
Heating Method ◽  
Freezing Method ◽  
Cold Area ◽  
Thermal Resources ◽  
Construction Joints ◽  
Secondary Lining ◽  
Effective Drainage

A new anti-freezing method for construction joints in tunnels is put forward through effective drainage ensured by heating with warm air or ribbon heater. The heating system is intelligently controlled according to the monitoring and analysis of the surface temperature of the secondary lining. This method is characterized as the following: (1) preventing leakage without pressure increase is realized through effective drainage at the back of secondary lining with special drainable channels; (2) alternative heating resources including warm air and electrical energy are offered to heat the region around construction joints, which can prevent the drainable channels from freezing and jamming; (3) for efficient and economy purpose, heating can be carried discontinuously as well as partly at circumferential and longitudinal direction. Numerical simulations validate the availability of heating method for anti-freezing.

scholarly journals Benchmarking DNA Extraction Methods for Phylogenomic Analysis of Sub-Antarctic Rhodococcus and Williamsia Species

2021 ◽  
Vol 9 (6) ◽  
pp. 1253
Author(s):  
Akhikun Nahar ◽  
Anthony L. Baker ◽  
David S. Nichols ◽  
John P. Bowman ◽  
Margaret L. Britz
Keyword(s):  
Dna Extraction ◽  
Classical Method ◽  
Fragment Size ◽  
Pairwise Comparison ◽  
Cell Lysis ◽  
Proteinase K ◽  
Phylogenomic Analysis ◽  
Rrna Gene ◽  
Spin Column ◽  
Rhodococcus Species

Bacteria containing mycolic acids in their cell envelope are often recalcitrant to cell lysis, so extracting DNA of sufficient quality for third-generation sequencing and high-fidelity genome assembly requires optimization, even when using commercial kits with protocols for hard-to-lyse bacteria. We benchmarked three spin-column-based kits against a classical DNA extraction method employing lysozyme, proteinase K and SDS for six lysozyme-resistant, sub-Antarctic strains of Corynebaceriales. Prior cultivation in broths containing glycine at highly growth-inhibitory concentrations (4.0–4.5%) improved cell lysis using both classical and kit methods. The classical method produced DNA with average fragment sizes of 27–59 Kbp and tight fragment size ranges, meeting quality standards for genome sequencing, assembly and phylogenomic analyses. By 16S rRNA gene sequencing, we classified two strains as Williamsia and four strains as Rhodococcus species. Pairwise comparison of average nucleotide identity (ANI) and alignment fraction (AF), plus genome clustering analysis, confirmed Rhodococcus sp. 1163 and 1168 and Williamsia sp. 1135 and 1138 as novel species. Phylogenetic, lipidomic and biochemical analyses classified psychrotrophic strains 1139 and 1159 as R. qingshengii and R. erythropolis, respectively, using ANI similarity of >98% and AF >60% for species delineation. On this basis, some members of the R. erythropolis genome cluster groups, including strains currently named as R. enclensis, R. baikonurensis, R. opacus and R. rhodochrous, would be reclassified either as R. erythropolis or R. qingshengii.

Report of the Blind Trial of the Cetus AmpliType HLA DQα Forensic Deoxyribonucleic Acid (DNA) Amplification and Typing Kit

1991 ◽  
Vol 36 (5) ◽  
pp. 13174J ◽  
Author(s):  
P. Sean Walsh ◽  
Nicola Fildes ◽  
Alan S. Louie ◽  
Russell Higuchi
Keyword(s):  
Deoxyribonucleic Acid ◽  
Dna Amplification ◽  
Blind Trial ◽  
Hla Dqα
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