Report of the Blind Trial of the Cetus AmpliType HLA DQα Forensic Deoxyribonucleic Acid (DNA) Amplification and Typing Kit

1991 ◽  
Vol 36 (5) ◽  
pp. 13174J ◽  
Author(s):  
P. Sean Walsh ◽  
Nicola Fildes ◽  
Alan S. Louie ◽  
Russell Higuchi
Keyword(s):  
Deoxyribonucleic Acid ◽  
Dna Amplification ◽  
Blind Trial ◽  
Hla Dqα

scholarly journals Fully Automated Lab-On-A-Disc Platform for Loop-Mediated Isothermal Amplification Using Micro-Carbon-Activated Cell Lysis

Sensors ◽  
2020 ◽  
Vol 20 (17) ◽  
pp. 4746
Author(s):  
Moo-Jung Seo ◽  
Jae-Chern Yoo
Keyword(s):  
Deoxyribonucleic Acid ◽  
Infrared Camera ◽  
Dna Amplification ◽  
Heating System ◽  
Cell Lysis ◽  
Heating Method ◽  
Standard Procedures ◽  
Spin Column ◽  
Full Automation ◽  
Real Temperature

Fast and fully automated deoxyribonucleic acid (DNA) amplification methods are of interest in the research on lab-on-a-disc (LOD) platforms because of their full compatibility with the spin-column mechanism using centrifugal force. However, the standard procedures followed in DNA amplification require accurate noncontact temperature control as well as cell lysis at a low temperature to prevent damage to the LOD platform. This requirement makes it challenging to achieve full automation of DNA amplification on an LOD. In this paper, a fully automated LOD capable of performing cell lysis and amplification on a single compact disc of DNA samples is proposed. The proposed system uses micro-carbon to heat DNA samples without damaging the LOD as well as a noncontact heating system and an infrared camera sensor to remotely measure the real temperature of the amplification chamber. Compared with conventional DNA amplification systems, the proposed system has the advantage of full automation of the LOD platform. Experimental results demonstrated that the proposed system offers a stable heating method for DNA amplification and cell lysis.

HLA-DQα Typing by DNA Amplification of Single Human Hair

1994 ◽  
pp. 349-350
Author(s):  
L. Caenazzo ◽  
C. Crestani ◽  
E. Ponzano ◽  
G. Bonan ◽  
P. Cortivo
Keyword(s):  
Human Hair ◽  
Dna Amplification ◽  
Hla Dqα

scholarly journals Genetic Profiling of Red-Bracted Cornus kousa Cultivars Indicates Significant Cultivar Synonomy

HortScience ◽  
2004 ◽  
Vol 39 (3) ◽  
pp. 489-492 ◽  
Author(s):  
R.N. Trigiano ◽  
M.H. Ament ◽  
M.T. Windham ◽  
J.K. Moulton
Keyword(s):  
Deoxyribonucleic Acid ◽  
Dna Amplification ◽  
Genetic Profiling ◽  
Flowering Dogwood ◽  
Landscape Plants ◽  
Cornus Kousa

Cornus kousa Hance (Korean or kousa dogwood) cultivars are increasingly used as landscape plants because they lack the disease and insect problems typically associated with the native C. florida L. (flowering dogwood). A number of red-bracted kousa dogwood cultivars are now available and several are phenotypically indistinguishable from one another. Plants of six cultivars obtained from three nurseries were characterized genetically using deoxyribonucleic acid (DNA) amplification fingerprinting (DAF) and arbitrary signatures from amplification profiles (ASAP). DAF profiles of three red-bracted cultivars—`Rosabella', `Satomi' and `Heart Throb'—were nearly identical. ASAP also failed to clearly differentiate these cultivars and indicated consistent genetic similarities. In contrast, another red-bracted cultivar `Christian Prince' and two white-bracted cultivars—`Little Beauty' and `Samaritan'—were identified and separated from all other cultivars by both DAF and ASAP techniques.

scholarly journals Secondary metabolites profiling of Vernonia amygdalina Del. in response to copper-induced abiotic stress

2021 ◽  
Vol 5 (1) ◽  
Author(s):  
Abosede Adu ◽  
Anthony Ojekale ◽  
Bamidele John ◽  
Opeyemi N. Avoseh
Keyword(s):  
Abiotic Stress ◽  
Secondary Metabolites ◽  
Inductively Coupled Plasma ◽  
Deoxyribonucleic Acid ◽  
Optical Emission ◽  
Dna Amplification ◽  
Inter Simple Sequence Repeat ◽  
Gas Chromatography Mass Spectrometry ◽  
Vernonia Amygdalina ◽  
Band Shift

Background: Vernonia amygdalina is a green leafy vegetable that grows in tropical Africa and popularly cultivated in the southern part of Nigeria for its economic, nutritional and ethnomedicine value.Aims: This study analysed the influence of copper-induced abiotic stress on the deoxyribonucleic acid and secondary metabolites of V. amygdalina.Setting: Analyses of plant material were carried out in the Department of Botany, Lagos State University.Methods: Deoxyribonucleic acid (DNA) extraction and Inter Simple Sequence Repeat (ISSR) amplification were performed. Copper (Cu) concentration in leaves and stem of V. amygdalina was determined using inductively coupled plasma-optical emission spectroscopy (ICP-OES), secondary metabolites were determined using gas chromatography–mass spectrometry (GC-MS) and the effect on DNA amplification and profiling was analysed using gel electrophoresis.Results: After 41 days of cultivation, the stem of V. amygdalina significantly bioaccumulated 37 mg/L, 50 mg/L and 215 mg/L of Cu after spiking with 400 mg/L, 1000 mg/L and 2000 mg/L, respectively, whilst the leaves accumulated 52 mg/L, 100 mg/L and 350 mg/L of Cu, respectively. Copper-impregnated soil influenced phytochemicals of the plants by causing inconsistent increase and decrease in specific compounds such as levomenthol, methyl stearate and glycerine. Deoxyribonucleic acid bands of the stem and leaf of V. amygdalina from control site showed no band shift, whilst band shift occurred in the stem and leaf of the Cu-spiked V. amygdalina.Conclusion: This study revealed the leaves of V. amygdalina having higher Cu accumulation capacity than the stems. Also, Cu alters the quality and quantity of phytochemicals in plant parts.

99: Prulifloxacin Versus Levofloxacin in the Treatment of Chronic Bacterial Prostatitis: A Prospective, Randomized, Double-Blind Trial

2007 ◽  
Vol 177 (4S) ◽  
pp. 35-35
Author(s):  
Gianluca Giannarini ◽  
Andrea Mogorovich ◽  
Girolamo Morelli ◽  
Maurizio De Maria ◽  
Francesca Manassero ◽  
...  
Keyword(s):  
Double Blind Trial ◽  
Chronic Bacterial Prostatitis ◽  
Double Blind ◽  
Blind Trial ◽  
Bacterial Prostatitis
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