scholarly journals Sensor Based on Molecularly Imprinted Polymer Membranes and Smartphone for Detection of Fusarium Contamination in Cereals

Sensors ◽  
2020 ◽  
Vol 20 (15) ◽  
pp. 4304
Author(s):  
Tetyana Sergeyeva ◽  
Daria Yarynka ◽  
Larysa Dubey ◽  
Igor Dubey ◽  
Elena Piletska ◽  
...  
Keyword(s):  
Molecularly Imprinted Polymer ◽  
Optical Sensors ◽  
Dynamic Range ◽  
Point Of Care ◽  
Field Measurements ◽  
Sensor System ◽  
Quality Analysis ◽  
Imprinted Polymer ◽  
Molecularly Imprinted ◽  
Smartphone Sensor

The combination of the generic mobile technology and inherent stability, versatility and cost-effectiveness of the synthetic receptors allows producing optical sensors for potentially any analyte of interest, and, therefore, to qualify as a platform technology for a fast routine analysis of a large number of contaminated samples. To support this statement, we present here a novel miniature sensor based on a combination of molecularly imprinted polymer (MIP) membranes and a smartphone, which could be used for the point-of-care detection of an important food contaminant, oestrogen-like toxin zearalenone associated with Fusarium contamination of cereals. The detection is based on registration of natural fluorescence of zearalenone using a digital smartphone camera after it binds to the sensor recognition element. The recorded image is further processed using a mobile application. It shows here a first example of the zearalenone-specific MIP membranes synthesised in situ using “dummy template”-based approach with cyclododecyl 2, 4-dihydroxybenzoate as the template and 1-allylpiperazine as a functional monomer. The novel smartphone sensor system based on optimized MIP membranes provides zearalenone detection in cereal samples within the range of 1–10 µg mL−1 demonstrating a detection limit of 1 µg mL−1 in a direct sensing mode. In order to reach the level of sensitivity required for practical application, a competitive sensing mode is also developed. It is based on application of a highly-fluorescent structural analogue of zearalenone (2-[(pyrene-l-carbonyl) amino]ethyl 2,4-dihydroxybenzoate) which is capable to compete with the target mycotoxin for the binding to zearalenone-selective sites in the membrane’s structure. The competitive mode increases 100 times the sensor’s sensitivity and allows detecting zearalenone at 10 ng mL−1. The linear dynamic range in this case comprised 10–100 ng mL−1. The sensor system is tested and found effective for zearalenone detection in maize, wheat and rye flour samples both spiked and naturally contaminated. The developed MIP membrane-based smartphone sensor system is an example of a novel, inexpensive tool for food quality analysis, which is portable and can be used for the “field” measurements and easily translated into the practice.

scholarly journals Molecularly Imprinted Polymer-Based Microfluidic Systems for Point-of-Care Applications

Micromachines ◽  
2019 ◽  
Vol 10 (11) ◽  
pp. 766 ◽  
Author(s):  
Yeşeren Saylan ◽  
Adil Denizli
Keyword(s):  
Molecularly Imprinted Polymer ◽  
High Stability ◽  
Point Of Care ◽  
Low Cost ◽  
Future Perspectives ◽  
Imprinted Polymer ◽  
Microfluidic Systems ◽  
Molecularly Imprinted ◽  
Wide Range ◽  
Physical And Chemical

Fast progress has been witnessed in the field of microfluidic systems and allowed outstanding approaches to portable, disposable, low-cost, and easy-to-operate platforms especially for monitoring health status and point-of-care applications. For this purpose, molecularly imprinted polymer (MIP)-based microfluidics systems can be synthesized using desired templates to create specific and selective cavities for interaction. This technique guarantees a wide range of versatility to imprint diverse sets of biomolecules with different structures, sizes, and physical and chemical features. Owing to their physical and chemical robustness, cost-friendliness, high stability, and reusability, MIP-based microfluidics systems have become very attractive modalities. This review is structured according to the principles of MIPs and microfluidic systems, the integration of MIPs with microfluidic systems, the latest strategies and uses for point-of-care applications and, finally, conclusions and future perspectives.

Molecularly Imprinted Polymer Nanoparticles for Selective Solid Phase Extraction of Fluvoxamine in Human Urine and Plasma

2019 ◽  
Vol 58 (3) ◽  
pp. 274-279
Author(s):  
Mojtaba Soleimani ◽  
Ameneh Porgham Daryasari ◽  
Parisa Joshani
Keyword(s):  
Molecularly Imprinted Polymer ◽  
Dynamic Range ◽  
Solid Phase ◽  
Polymer Nanoparticles ◽  
Template Molecule ◽  
Imprinted Polymer ◽  
Molecularly Imprinted ◽  
Selective Determination ◽  
Ft Ir

Abstract In this work, the molecularly imprinted polymer nanoparticles (MIP-NPs) for the selective determination of fluvoxamine have been described. The polymer nanoparticles were synthesized by the polymerization of methacrylic acid as a functional monomer, ethylene glycol dimethacrylate as a cross-linker, 2,2-azobisisobutyronitrile as an initiator and fluvoxamine as a template molecule. The MIP-NPs were characterized using techniques that included Fourier transform infrared (FT-IR) spectroscopy and scanning electron microscopy (SEM). Imprinted fluvoxamine molecules were removed from the polymeric structure using acetonitrile in methanol (2:8; v/v) as the eluting solvent. The linear dynamic range for fluvoxamine was 10–1200 μg L−1. The developed method was successfully applied to the extraction of fluvoxamine in complex biological samples.

scholarly journals Reagentless Sensing of Vancomycin Using an Indium Tin Oxide Electrode Grafted with Molecularly Imprinted Polymer including Ferrocenyl Group

Sensors ◽  
2021 ◽  
Vol 21 (24) ◽  
pp. 8338
Author(s):  
Haruto Eguchi ◽  
Akihiko Hatano ◽  
Yasuo Yoshimi
Keyword(s):  
Tin Oxide ◽  
Molecularly Imprinted Polymer ◽  
Indium Tin Oxide ◽  
Dynamic Range ◽  
Therapeutic Drug ◽  
Background Current ◽  
Imprinted Polymer ◽  
High Background ◽  
Molecularly Imprinted ◽  
Ito Electrode

Vancomycin (VCM) is a first-line antimicrobial agent against methicillin-resistant Staphylococcus aureus, a cause of nosocomial infections. Therapeutic drug monitoring is strongly recommended for VCM-based chemotherapy. The authors attempted to develop a simple VCM sensor based on molecularly imprinted polymer (MIP), which can be used with simple operations. Methacrylic acid (MAA), acrylamide, methylenebisacrylamide, and allylamine carboxypropionate-3-ferrocene (ACPF) were copolymerized in the presence of VCM and grafted from the surface of indium-tin oxide (ITO) to obtain MIP-coated electrodes. The MIP-grafted ITO electrode was used for differential pulse voltammetry (DPV) measurements in a buffer solution containing VCM or whole bovine blood. The obtained current depends on the VCM concentration with high linearity. The dynamic range covered the therapeutic range (20–40 μg/mL) of the VCM but was almost insensitive to teicoplanin, which has a similar structure to VCM. The ITO electrodes grafted by the same procedure except for omitting either VCM or APCF were not sensitive to VCM. The sensitivity of the MIP electrodes to VCM in whole blood and buffered saline, but the background current in blood was higher than that in saline. This high background current was also seen in the deproteinized plasma. Thus, the current is probably originated from the oxidation of low molecular weight reducing agents in the blood. The MIP-grafted ITO electrode using ACPF as a functional monomer would be a promising highly selective sensor for real-time monitoring of VCM with proper correction of the background current.

scholarly journals Graphite-Polyurethane Composite Electrode Modified with Molecularly Imprinted Polymer for Determination of Diclofenac

2021 ◽  
Author(s):  
Abigail Pereira ◽  
Priscila Cervini ◽  
Victor Rivera ◽  
Éder Cavalheiro
Keyword(s):  
Electron Microscopy ◽  
Molecularly Imprinted Polymer ◽  
Dynamic Range ◽  
Limit Of Detection ◽  
Anodic Stripping Voltammetry ◽  
Saturated Calomel Electrode ◽  
Pulse Amplitude ◽  
Imprinted Polymer ◽  
Molecularly Imprinted ◽  
Polyurethane Composite

A molecularly imprinted polymer (MIP) was prepared using the anti-inflammatory diclofenac (DCF) as a template. A non-imprinted polymer (NIP) was also prepared as a control. These MIP and NIP were characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM) and Brunauer-Emmett-Teller (BET), revealing a higher porosity in the first. Then both were used in the modification of graphite-polyurethane composites electrodes (GPUE). Differential pulse anodic stripping voltammetry was used for DCF determination at GPUE-MIP-DCF containing 2.5% (m/m) of the modifier in perchloric acid, pH = 2.0, after previously optimized conditions such as 300 s of accumulation time, +0.2 V accumulation potential (vs. SCE (saturated calomel electrode)), 50 mV pulse amplitude and 10 mV s–1 scan rate. A linear dynamic range from 0.010 to 0.20 μmol L–1 and a limit of detection (LOD) of 0.99 nmol L–1 were found, using GPUE-2.5-MIP-DCF. DCF was determined in commercial pharmaceutical formulations and in synthetic urine samples, with recoveries between 101 and 102% (n = 3) and 101% (n = 3), respectively. The results agreed with the reference high-performance liquid chromatography (HPLC) within 95% confidence level, according to Student’s t-test. Interference from meclofenamic and mefenamic acids, which are structurally similar to DCF, was also evaluated. Interferences could not be totally avoided, but MIPs presented a considerable ability in discriminating the voltammetric response for DFC, despite the close structural similarity with the interferents.

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