scholarly journals Molecularly Imprinted Polymer-Based Microfluidic Systems for Point-of-Care Applications

Micromachines ◽  
2019 ◽  
Vol 10 (11) ◽  
pp. 766 ◽  
Author(s):  
Yeşeren Saylan ◽  
Adil Denizli
Keyword(s):  
Molecularly Imprinted Polymer ◽  
High Stability ◽  
Point Of Care ◽  
Low Cost ◽  
Future Perspectives ◽  
Imprinted Polymer ◽  
Microfluidic Systems ◽  
Molecularly Imprinted ◽  
Wide Range ◽  
Physical And Chemical

Fast progress has been witnessed in the field of microfluidic systems and allowed outstanding approaches to portable, disposable, low-cost, and easy-to-operate platforms especially for monitoring health status and point-of-care applications. For this purpose, molecularly imprinted polymer (MIP)-based microfluidics systems can be synthesized using desired templates to create specific and selective cavities for interaction. This technique guarantees a wide range of versatility to imprint diverse sets of biomolecules with different structures, sizes, and physical and chemical features. Owing to their physical and chemical robustness, cost-friendliness, high stability, and reusability, MIP-based microfluidics systems have become very attractive modalities. This review is structured according to the principles of MIPs and microfluidic systems, the integration of MIPs with microfluidic systems, the latest strategies and uses for point-of-care applications and, finally, conclusions and future perspectives.

scholarly journals Molecularly Imprinted Polymer Based Sensors for Medical Applications

Sensors ◽  
2019 ◽  
Vol 19 (6) ◽  
pp. 1279 ◽  
Author(s):  
Yeşeren Saylan ◽  
Semra Akgönüllü ◽  
Handan Yavuz ◽  
Serhat Ünal ◽  
Adil Denizli
Keyword(s):  
Surface Modification ◽  
Homeland Security ◽  
Molecular Imprinting ◽  
Molecularly Imprinted Polymer ◽  
Structural Changes ◽  
Low Cost ◽  
Dimensional Structure ◽  
Imprinted Polymer ◽  
Molecularly Imprinted ◽  
Physical And Chemical

Sensors have been extensively used owing to multiple advantages, including exceptional sensing performance, user-friendly operation, fast response, high sensitivity and specificity, portability, and real-time analysis. In recent years, efforts in sensor realm have expanded promptly, and it has already presented a broad range of applications in the fields of medical, pharmaceutical and environmental applications, food safety, and homeland security. In particular, molecularly imprinted polymer based sensors have created a fascinating horizon for surface modification techniques by forming specific recognition cavities for template molecules in the polymeric matrix. This method ensures a broad range of versatility to imprint a variety of biomolecules with different size, three dimensional structure, physical and chemical features. In contrast to complex and time-consuming laboratory surface modification methods, molecular imprinting offers a rapid, sensitive, inexpensive, easy-to-use, and highly selective approaches for sensing, and especially for the applications of diagnosis, screening, and theranostics. Due to its physical and chemical robustness, high stability, low-cost, and reusability features, molecularly imprinted polymer based sensors have become very attractive modalities for such applications with a sensitivity of minute structural changes in the structure of biomolecules. This review aims at discussing the principle of molecular imprinting method, the integration of molecularly imprinted polymers with sensing tools, the recent advances and strategies in molecular imprinting methodologies, their applications in medical, and future outlook on this concept.

scholarly journals Recent Advances and Perspectives of Molecularly Imprinted Polymer-Based Fluorescent Sensors in Food and Environment Analysis

Nanomaterials ◽  
2019 ◽  
Vol 9 (7) ◽  
pp. 1030 ◽  
Author(s):  
Guangyang Liu ◽  
Xiaodong Huang ◽  
Lingyun Li ◽  
Xiaomin Xu ◽  
Yanguo Zhang ◽  
...  
Keyword(s):  
Molecularly Imprinted Polymer ◽  
New Technology ◽  
Three Dimensional ◽  
High Sensitivity ◽  
Detection Methods ◽  
Fluorescent Nanoparticles ◽  
Imprinted Polymer ◽  
Recognition Mechanism ◽  
Molecularly Imprinted ◽  
Wide Range

Molecular imprinting technology (MIT), also known as molecular template technology, is a new technology involving material chemistry, polymer chemistry, biochemistry, and other multi-disciplinary approaches. This technology is used to realize the unique recognition ability of three-dimensional crosslinked polymers, called the molecularly imprinted polymers (MIPs). MIPs demonstrate a wide range of applicability, good plasticity, stability, and high selectivity, and their internal recognition sites can be selectively combined with template molecules to achieve selective recognition. A molecularly imprinted fluorescence sensor (MIFs) incorporates fluorescent materials (fluorescein or fluorescent nanoparticles) into a molecularly imprinted polymer synthesis system and transforms the binding sites between target molecules and molecularly imprinted materials into readable fluorescence signals. This sensor demonstrates the advantages of high sensitivity and selectivity of fluorescence detection. Molecularly imprinted materials demonstrate considerable research significance and broad application prospects. They are a research hotspot in the field of food and environment safety sensing analysis. In this study, the progress in the construction and application of MIFs was reviewed with emphasis on the preparation principle, detection methods, and molecular recognition mechanism. The applications of MIFs in food and environment safety detection in recent years were summarized, and the research trends and development prospects of MIFs were discussed.

scholarly journals CdTe0.5S0.5/ZnS Quantum Dots Embedded in a Molecularly Imprinted Polymer for the Selective Optosensing of Dopamine

Nanomaterials ◽  
2019 ◽  
Vol 9 (5) ◽  
pp. 693 ◽  
Author(s):  
Kiana Khadem-Abbassi ◽  
Hervé Rinnert ◽  
Lavinia Balan ◽  
Zahra Doumandji ◽  
Olivier Joubert ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Ethylene Glycol ◽  
Molecularly Imprinted Polymer ◽  
Limit Of Detection ◽  
Low Cost ◽  
Core Shell ◽  
Selective Detection ◽  
Imprinted Polymer ◽  
Molecularly Imprinted ◽  
Cross Linker

This work describes the preparation of molecularly imprinted polymer (MIP)-modified core/shell CdTe0.5S0.5/ZnS quantum dots (QDs). The QDs@MIP particles were used for the selective and sensitive detection of dopamine (DA). Acrylamide, which is able to form hydrogen bonds with DA, and ethylene glycol dimethylacrylate (EGDMA) as cross-linker were used for the preparation of the MIP. Highly cross-linked polymer particles with sizes up to 1 µm containing the dots were obtained after the polymerization. After the removal of the DA template, MIP-modified QDs (QDs@MIP) exhibit a high photoluminescence (PL) with an intensity similar to that of QDs embedded in the nonimprinted polymer (NIP). A linear PL decrease was observed upon addition of DA to QDs@MIP and the PL response was in the linear ranges from 2.63 µM to 26.30 µM with a limit of detection of 6.6 nM. The PL intensity of QDs@MIP was quenched selectively by DA. The QDs@MIP particles developed in this work are easily prepared and of low cost and are therefore of high interest for the sensitive and selective detection of DA in biological samples.

scholarly journals Detection of Small Peptide Hormones for Anti-Doping Purpose via a Molecularly Imprinted Polymer-Based SPR Assay

Proceedings ◽  
2020 ◽  
Vol 60 (1) ◽  
pp. 56
Author(s):  
Francesca Torrini ◽  
Pasquale Palladino ◽  
Simona Scarano ◽  
Maria Minunni
Keyword(s):  
Molecularly Imprinted Polymer ◽  
Bioanalytical Methods ◽  
Low Cost ◽  
Peptide Hormones ◽  
Imprinted Polymer ◽  
Small Peptide ◽  
Molecularly Imprinted

Currently, there is a lack of low-cost, prompt and robust bioanalytical methods to detect smallpeptide hormones (e.g., gonadorelin, buserelin, leuprorelin, etc.) in the routine anti-doping protocol. [...]

Advances in Molecularly Imprinted Systems: Materials, Characterization Methods and Analytical Applications

2020 ◽  
Vol 16 (3) ◽  
pp. 196-207 ◽  
Author(s):  
Yeşeren Saylan ◽  
Adil Denizli
Keyword(s):  
Molecular Imprinting ◽  
Molecularly Imprinted Polymers ◽  
High Stability ◽  
Low Cost ◽  
Three Dimensional ◽  
Molecularly Imprinted ◽  
Imprinted Polymers ◽  
Wide Range ◽  
Physicochemical Features ◽  
Target Molecules

Introduction: A molecular imprinting is one of the fascinating modification methods that employ molecules as targets to create geometric cavities for recognition of targets in the polymeric matrix. This method provides a broad versatility to imprint target molecules with different size, three-dimensional structure and physicochemical features. In contrast to the complex and timeconsuming laboratory surface modification procedures, this method offers a rapid, sensitive, inexpensive, easy-to-use, and selective approach for the diagnosis, screening and monitoring disorders. Owing to their unique features such as high selectivity, physical and chemical robustness, high stability, low-cost and reusability of this method, molecularly imprinted polymers have become very attractive materials and been applied in various applications from separation to detection. Background: The aims of this review are structured according to the fundamentals of molecularly imprinted polymers involving essential elements, preparation procedures and also the analytical applications platforms. Finally, the future perspectives to increase the development of molecularly imprinted platforms. Methods: A molecular imprinting is one of the commonly used modification methods that apply target as a recognition element itself and provide a wide range of versatility to replica other targets with a different structure, size, and physicochemical features. A rapid, easy, cheap and specific recognition approach has become one of the investigation areas on, especially biochemistry, biomedicine and biotechnology. In recent years, several technologies of molecular imprinting method have gained prompt development according to continuous use and improvement of traditional polymerization techniques. Results: The molecularly imprinted polymers with excellent performances have been prepared and also more exciting and universal applications have been recognized. In contrast to the conventional methods, the imprinted systems have superior advantages including high stability, relative ease and low cost of preparation, resistance to elevated temperature, and pressure and potential application to various target molecules. In view of these considerations, molecularly imprinted systems have found application in various fields of analytical chemistry including separation, purification, detection and spectrophotometric systems. Conclusion: Recent analytical methods are reported to develop the binding kinetics of imprinted systems by using the development of other technologies. The combined platforms are among the most encouraging systems to detect and recognize several molecules. The diversity of molecular imprinting methods was overviewed for different analytical application platforms. There is still a requirement of more knowledge on the molecular features of these polymers. A next step would further be the optimization of different systems with more homogeneous and easily reachable recognition sites to reduce the laborious in the accessibility in the three-dimensional polymeric materials in sufficient recognition features and also better selectivity and sensitivity for a wide range of molecules.

scholarly journals Laser-Induced Graphene Electrodes Modified with a Molecularly Imprinted Polymer for Detection of Tetracycline in Milk and Meat

Sensors ◽  
2021 ◽  
Vol 22 (1) ◽  
pp. 269
Author(s):  
Biresaw D. Abera ◽  
Inmaculada Ortiz-Gómez ◽  
Bajramshahe Shkodra ◽  
Francisco J. Romero ◽  
Giuseppe Cantarella ◽  
...  
Keyword(s):  
Molecularly Imprinted Polymer ◽  
Limit Of Detection ◽  
Low Cost ◽  
Electrochemical Techniques ◽  
Differential Pulse ◽  
Animal Origin ◽  
Label Free ◽  
Imprinted Polymer ◽  
Meat Extract ◽  
Molecularly Imprinted

Tetracycline (TC) is a widely known antibiotic used worldwide to ‘’treat animals. Its residues in animal-origin foods cause adverse health effects to consumers. Low-cost and real-time measuring systems of TC in food samples are, therefore, extremely needed. In this work, a three-electrode sensitive and label-free sensor was developed to detect TC residues from milk and meat extract samples, using CO2 laser-induced graphene (LIG) electrodes modified with gold nanoparticles (AuNPs) and a molecularly imprinted polymer (MIP) used as a synthetic biorecognition element. LIG was patterned on a polyimide (PI) substrate, reaching a minimum sheet resistance (Rsh) of 17.27 ± 1.04 Ω/sq. The o-phenylenediamine (oPD) monomer and TC template were electropolymerized on the surface of the LIG working electrode to form the MIP. Surface morphology and electrochemical techniques were used to characterize the formation of LIG and to confirm each modification step. The sensitivity of the sensor was evaluated by differential pulse voltammetry (DPV), leading to a limit of detection (LOD) of 0.32 nM, 0.85 nM, and 0.80 nM in buffer, milk, and meat extract samples, respectively, with a working range of 5 nM to 500 nM and a linear response range between 10 nM to 300 nM. The sensor showed good LOD (0.32 nM), reproducibility, and stability, and it can be used as an alternative system to detect TC from animal-origin food products.

scholarly journals Protein Determination with Molecularly Imprinted Polymer Recognition Combined with Birefringence Liquid Crystal Detection

Sensors ◽  
2020 ◽  
Vol 20 (17) ◽  
pp. 4692 ◽  
Author(s):  
Maciej Cieplak ◽  
Rafał Węgłowski ◽  
Zofia Iskierko ◽  
Dorota Węgłowska ◽  
Piyush S. Sharma ◽  
...  
Keyword(s):  
Liquid Crystal ◽  
Molecularly Imprinted Polymer ◽  
Limit Of Detection ◽  
Low Cost ◽  
High Sensitivity ◽  
Imprinted Polymer ◽  
Optical Cell ◽  
Protein Determination ◽  
Molecularly Imprinted ◽  
Nonspecific Interactions

Liquid crystal-based sensors offer the advantage of high sensitivity at a low cost. However, they often lack selectivity altogether or require costly and unstable biomaterials to impart this selectivity. To incur this selectivity, we herein integrated a molecularly imprinted polymer (MIP) film recognition unit with a liquid crystal (LC) in an optical cell transducer. We tested the resulting chemosensor for protein determination. We examined two different LCs, each with a different optical birefringence. That way, we revealed the influence of that parameter on the sensitivity of the (human serum albumin)-templated (MIP-HSA) LC chemosensor. The response of this chemosensor with the (MIP-HSA)-recognizing film was linear from 2.2 to 15.2 µM HSA, with a limit of detection of 2.2 µM. These values are sufficient to use the devised chemosensor for HSA determination in biological samples. Importantly, the imprinting factor (IF) of this chemosensor was appreciable, reaching IF = 3.7. This IF value indicated the predominant binding of the HSA through specific rather than nonspecific interactions with the MIP.

scholarly journals Sensor Based on Molecularly Imprinted Polymer Membranes and Smartphone for Detection of Fusarium Contamination in Cereals

Sensors ◽  
2020 ◽  
Vol 20 (15) ◽  
pp. 4304
Author(s):  
Tetyana Sergeyeva ◽  
Daria Yarynka ◽  
Larysa Dubey ◽  
Igor Dubey ◽  
Elena Piletska ◽  
...  
Keyword(s):  
Molecularly Imprinted Polymer ◽  
Optical Sensors ◽  
Dynamic Range ◽  
Point Of Care ◽  
Field Measurements ◽  
Sensor System ◽  
Quality Analysis ◽  
Imprinted Polymer ◽  
Molecularly Imprinted ◽  
Smartphone Sensor

The combination of the generic mobile technology and inherent stability, versatility and cost-effectiveness of the synthetic receptors allows producing optical sensors for potentially any analyte of interest, and, therefore, to qualify as a platform technology for a fast routine analysis of a large number of contaminated samples. To support this statement, we present here a novel miniature sensor based on a combination of molecularly imprinted polymer (MIP) membranes and a smartphone, which could be used for the point-of-care detection of an important food contaminant, oestrogen-like toxin zearalenone associated with Fusarium contamination of cereals. The detection is based on registration of natural fluorescence of zearalenone using a digital smartphone camera after it binds to the sensor recognition element. The recorded image is further processed using a mobile application. It shows here a first example of the zearalenone-specific MIP membranes synthesised in situ using “dummy template”-based approach with cyclododecyl 2, 4-dihydroxybenzoate as the template and 1-allylpiperazine as a functional monomer. The novel smartphone sensor system based on optimized MIP membranes provides zearalenone detection in cereal samples within the range of 1–10 µg mL−1 demonstrating a detection limit of 1 µg mL−1 in a direct sensing mode. In order to reach the level of sensitivity required for practical application, a competitive sensing mode is also developed. It is based on application of a highly-fluorescent structural analogue of zearalenone (2-[(pyrene-l-carbonyl) amino]ethyl 2,4-dihydroxybenzoate) which is capable to compete with the target mycotoxin for the binding to zearalenone-selective sites in the membrane’s structure. The competitive mode increases 100 times the sensor’s sensitivity and allows detecting zearalenone at 10 ng mL−1. The linear dynamic range in this case comprised 10–100 ng mL−1. The sensor system is tested and found effective for zearalenone detection in maize, wheat and rye flour samples both spiked and naturally contaminated. The developed MIP membrane-based smartphone sensor system is an example of a novel, inexpensive tool for food quality analysis, which is portable and can be used for the “field” measurements and easily translated into the practice.

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