scholarly journals Antibody-Electroactive Probe Conjugates Based Electrochemical Immunosensors

Sensors ◽  
2020 ◽  
Vol 20 (7) ◽  
pp. 2014 ◽  
Author(s):  
Mateusz Kondzior ◽  
Iwona Grabowska
Keyword(s):  
Horseradish Peroxidase ◽  
Electrochemical Immunosensor ◽  
Signal Generation ◽  
Sensor Signal ◽  
Redox Probe ◽  
Direct Signal ◽  
Redox Active ◽  
Electrochemical Immunosensors ◽  
Analyte Detection ◽  
Transducer Surface

Suitable immobilization of a biorecognition element, such as an antigen or antibody, on a transducer surface is essential for development of sensitive and analytically reliable immunosensors. In this review, we report on (1) methods of antibody prefunctionalization using electroactive probes, (2) methods for immobilization of such conjugates on the surfaces of electrodes in electrochemical immunosensor construction and (3) the use of antibody-electroactive probe conjugates as bioreceptors and sensor signal generators. We focus on different strategies of antibody functionalization using the redox active probes ferrocene (Fc), anthraquinone (AQ), thionine (Thi), cobalt(III) bipyridine (Co(bpy)33+), Ru(bpy)32+ and horseradish peroxidase (HRP). In addition, new possibilities for antibody functionalization based on bioconjugation techniques are presented. We discuss strategies of specific, quantitative antigen detection based on (i) a sandwich format and (ii) a direct signal generation scheme. Further, the integration of different nanomaterials in the construction of these immunosensors is presented. Lastly, we report the use of a redox probe strategy in multiplexed analyte detection.

Infrared sensor signal generation method for direct signal injection simulation

2015 ◽  
Vol 54 (8) ◽  
pp. 084101 ◽  
Author(s):  
Dong-Yang Zhang ◽  
Jian-Qi Zhang ◽  
Xiao-Rui Wang ◽  
Guo-Jing He
Keyword(s):  
Signal Generation ◽  
Infrared Sensor ◽  
Sensor Signal ◽  
Direct Signal ◽  
Injection Simulation ◽  
Signal Injection

Electrochemical Immunosensors Based on Nanostructured Materials for Sensing of Prostate-Specific Antigen: A Review

2020 ◽  
Vol 28 ◽  
Author(s):  
Hayati Filik ◽  
Asiye Aslıhan Avan ◽  
Mustafa Özyürek
Keyword(s):  
Prostate Specific Antigen ◽  
Nanostructured Materials ◽  
Specific Antigen ◽  
Electrochemical Immunosensor ◽  
Label Free ◽  
Electrochemical Immunosensors ◽  
Metal Metal ◽  
Different Types ◽  
Sandwich Type ◽  
Carbon Based Nanomaterials

: The prostate-specific antigen (PSA) has been considered a crucial serological marker for distinguishing prostate based cancer. This surveys recent progress in the construction of nanomaterial-based electrochemical immunosensors for a PSA. This review (from 2015 to 2020) reports the latest progress in PSA sensing based on the employ of different types of nanostructured materials. The most popular used nanostructured materials are metal, metal oxide, carbon-based nanomaterials, and their hybrid architectures utilized for distinct amplification protocols. In this review, the electrochemical immunosensors for prostate-specific antigen sensing are classified into three categories such as sandwich type@labeled, label free@nonlabeled and aptamer-based electrochemical immunosensor.

scholarly journals Metal Nanoparticle and Quantum Dot Tags for Signal Amplification in Electrochemical Immunosensors for Biomarker Detection

Chemosensors ◽  
2021 ◽  
Vol 9 (4) ◽  
pp. 85
Author(s):  
Anton Popov ◽  
Benediktas Brasiunas ◽  
Asta Kausaite-Minkstimiene ◽  
Almira Ramanaviciene
Keyword(s):  
Signal Amplification ◽  
Electrochemical Immunosensor ◽  
Metal Nanoparticle ◽  
Biomarker Detection ◽  
Analytical Instruments ◽  
Electrochemical Immunosensors ◽  
Highly Sensitive ◽  
Recent Developments ◽  
Electrochemical Signal ◽  
Detection Of Biomarkers

With the increasing importance of healthcare and clinical diagnosis, as well as the growing demand for highly sensitive analytical instruments, immunosensors have received considerable attention. In this review, electrochemical immunosensor signal amplification strategies using metal nanoparticles (MNPs) and quantum dots (Qdots) as tags are overviewed, focusing on recent developments in the ultrasensitive detection of biomarkers. MNPs and Qdots can be used separately or in combination with other nanostructures, while performing the function of nanocarriers, electroactive labels, or catalysts. Thus, different functions of MNPs and Qdots as well as recent advances in electrochemical signal amplification are discussed. Additionally, the methods most often used for antibody immobilization on nanoparticles, immunoassay formats, and electrochemical methods for indirect biomarker detection are overviewed.

scholarly journals Fabrication of a label-free electrochemical immunosensor using a redox active ferrocenyl dendrimer

2016 ◽  
Vol 40 (11) ◽  
pp. 9046-9053 ◽  
Author(s):  
Sudeshna Chandra ◽  
Christian Gäbler ◽  
Christian Schliebe ◽  
Heinrich Lang ◽  
Dhirendra Bahadur
Keyword(s):  
Pamam Dendrimer ◽  
Electrochemical Immunosensor ◽  
Sensitive Detection ◽  
Label Free ◽  
Amperometric Immunosensor ◽  
Redox Active ◽  
Highly Sensitive ◽  
Highly Sensitive Detection

An amperometric immunosensor based on a redox active ferrocenyl end-grafted PAMAM dendrimer provides highly sensitive detection of immunoglobulin, down to 2 ng mL−1.

scholarly journals Dendrimers as Soft Nanomaterials for Electrochemical Immunosensors

Nanomaterials ◽  
2019 ◽  
Vol 9 (12) ◽  
pp. 1745 ◽  
Author(s):  
Alfredo Sánchez ◽  
Anabel Villalonga ◽  
Gonzalo Martínez-García ◽  
Concepción Parrado ◽  
Reynaldo Villalonga
Keyword(s):  
Pharmaceutical Analysis ◽  
State Of The Art ◽  
Three Dimensional ◽  
Electrochemical Immunosensor ◽  
High Impact ◽  
Branched Polymers ◽  
Electrochemical Immunosensors ◽  
Signal Production ◽  
Poly Propylene ◽  
Affinity Biosensors

Electrochemical immunosensors are antibody-based affinity biosensors with a high impact on clinical, environmental, food, and pharmaceutical analysis. In general, the analytical performance of these devices is critically determined by the materials and reagents used for their construction, signal production and amplification. Dendrimers are monodisperse and highly branched polymers with three-dimensional structures widely employed as “soft” nanomaterials in electrochemical immunosensor technology. This review provides an overview on the state-of-the-art in dendrimer-based electrochemical immunosensors, focusing on those using polyamidoamine and poly (propylene imine) dendrimers. Special emphasis is given to the most original methods recently reported for the construction of immunosensor architectures incorporating dendrimers, as well as to novel sensing approaches based on dendrimer-assisted signal enhancement strategies.

Streptavidin-polypyrrole Film as Platform for Biotinylated Redox Probe Immobilization for Electrochemical Immunosensor Application

2016 ◽  
Vol 28 (8) ◽  
pp. 1824-1832 ◽  
Author(s):  
Anna Miodek ◽  
Huu Quynh Anh Lê ◽  
Helene Sauriat-Dorizon ◽  
Hafsa Korri-Youssoufi
Keyword(s):  
Electrochemical Immunosensor ◽  
Redox Probe ◽  
Polypyrrole Film

scholarly journals Electrochemical DNA sensors based on spatially distributed redox mediators: challenges and promises

2017 ◽  
Vol 89 (10) ◽  
pp. 1471-1490 ◽  
Author(s):  
Gennady A. Evtugyn ◽  
Anna V. Porfireva ◽  
Ivan I. Stoikov
Keyword(s):  
Conductive Polymers ◽  
Signal Generation ◽  
Dna Aptamers ◽  
Dna Sensors ◽  
Redox Mediators ◽  
Disease Biomarkers ◽  
Redox Active ◽  
Reliable Detection ◽  
Spatially Distributed ◽  
Biomedical Diagnostics

AbstractDNA and aptasensors are widely used for fast and reliable detection of disease biomarkers, pharmaceuticals, toxins, metabolites and other species necessary for biomedical diagnostics. In the overview, the concept of spatially distributed redox mediators is considered with particular emphasis to the signal generation and biospecific layer assembling. The application of non-conductive polymers bearing redox labels, supramolecular carriers with attached DNA aptamers and redox active dyes and E-sensor concept are considered as examples of the approach announced.

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