Streptavidin-polypyrrole Film as Platform for Biotinylated Redox Probe Immobilization for Electrochemical Immunosensor Application

2016 ◽  
Vol 28 (8) ◽  
pp. 1824-1832 ◽  
Author(s):  
Anna Miodek ◽  
Huu Quynh Anh Lê ◽  
Helene Sauriat-Dorizon ◽  
Hafsa Korri-Youssoufi
Keyword(s):  
Electrochemical Immunosensor ◽  
Redox Probe ◽  
Polypyrrole Film

A label-free electrochemical bisphenol A immunosensor based on chlorogenic acid as a redox probe

2017 ◽  
Vol 9 (14) ◽  
pp. 2183-2188 ◽  
Author(s):  
Xiaofeng Li ◽  
Ying Huang ◽  
Mei Chen ◽  
Yuejin Tong ◽  
Cuiyun Zhang
Keyword(s):  
Gold Nanoparticles ◽  
Bisphenol A ◽  
Chlorogenic Acid ◽  
Electrochemical Immunosensor ◽  
Acetylene Black ◽  
Label Free ◽  
Redox Probe

A simple and sensitive electrochemical immunosensor for bisphenol A was prepared with an acetylene black–chitosan–gold composite (AB–CS–Au) and gold nanoparticles (AuNPs) as electrode modifiers and chlorogenic acid (CGA) as the redox probe.

scholarly journals SYNTHESIS OF BIOTINYLATED FERROCENE, AND ITS APPLICATION AS A REDOX PROBE FOR ELECTROCHEMICAL IMMUNOSENSOR

2018 ◽  
Vol 54 (2C) ◽  
pp. 441
Author(s):  
Le Huu Quynh Anh
Keyword(s):  
Detection Limit ◽  
13C Nmr ◽  
Differential Pulse ◽  
Electrochemical Immunosensor ◽  
Redox Properties ◽  
Structure Identification ◽  
Redox Probe ◽  
Pulse Voltammetry ◽  
Antigen Interaction ◽  
Derivatives Of

The coupling compound of Biotin (Vitamin H) and redox molecule ferrocene throughcovalent bonding was studied. Synthesis and structure identification of Biotinylated 1 - (- 2-aminoethyl) ferrocene were performed by 1H-NMR, 13C-NMR and MS. The ultimate goal of thispaper is to develop derivatives of ferroceneservingas an electrochemical probe that wouldenhance the performance of immunosensor.An immunosensor based on polypyrrole-streptavidinlayer as platform for immobilization of biotinylatedferrocene and antibodies was developed. Themeasurement of redox signal of ferrocene was analyzed by differential pulse voltammetry(DPV) method and underlined a variation of redox properties upon the antigen interaction. Theintroduction of the redox biotinylatedferrocene into streptavidin-polypyrrole layer affords a goodsensitivity of the biosensor with a detection limit of 0.16 pg.mL-1.

scholarly journals Antibody-Electroactive Probe Conjugates Based Electrochemical Immunosensors

Sensors ◽  
2020 ◽  
Vol 20 (7) ◽  
pp. 2014 ◽  
Author(s):  
Mateusz Kondzior ◽  
Iwona Grabowska
Keyword(s):  
Horseradish Peroxidase ◽  
Electrochemical Immunosensor ◽  
Signal Generation ◽  
Sensor Signal ◽  
Redox Probe ◽  
Direct Signal ◽  
Redox Active ◽  
Electrochemical Immunosensors ◽  
Analyte Detection ◽  
Transducer Surface

Suitable immobilization of a biorecognition element, such as an antigen or antibody, on a transducer surface is essential for development of sensitive and analytically reliable immunosensors. In this review, we report on (1) methods of antibody prefunctionalization using electroactive probes, (2) methods for immobilization of such conjugates on the surfaces of electrodes in electrochemical immunosensor construction and (3) the use of antibody-electroactive probe conjugates as bioreceptors and sensor signal generators. We focus on different strategies of antibody functionalization using the redox active probes ferrocene (Fc), anthraquinone (AQ), thionine (Thi), cobalt(III) bipyridine (Co(bpy)33+), Ru(bpy)32+ and horseradish peroxidase (HRP). In addition, new possibilities for antibody functionalization based on bioconjugation techniques are presented. We discuss strategies of specific, quantitative antigen detection based on (i) a sandwich format and (ii) a direct signal generation scheme. Further, the integration of different nanomaterials in the construction of these immunosensors is presented. Lastly, we report the use of a redox probe strategy in multiplexed analyte detection.

Electrochemical Immunosensors Based on Nanostructured Materials for Sensing of Prostate-Specific Antigen: A Review

2020 ◽  
Vol 28 ◽  
Author(s):  
Hayati Filik ◽  
Asiye Aslıhan Avan ◽  
Mustafa Özyürek
Keyword(s):  
Prostate Specific Antigen ◽  
Nanostructured Materials ◽  
Specific Antigen ◽  
Electrochemical Immunosensor ◽  
Label Free ◽  
Electrochemical Immunosensors ◽  
Metal Metal ◽  
Different Types ◽  
Sandwich Type ◽  
Carbon Based Nanomaterials

: The prostate-specific antigen (PSA) has been considered a crucial serological marker for distinguishing prostate based cancer. This surveys recent progress in the construction of nanomaterial-based electrochemical immunosensors for a PSA. This review (from 2015 to 2020) reports the latest progress in PSA sensing based on the employ of different types of nanostructured materials. The most popular used nanostructured materials are metal, metal oxide, carbon-based nanomaterials, and their hybrid architectures utilized for distinct amplification protocols. In this review, the electrochemical immunosensors for prostate-specific antigen sensing are classified into three categories such as sandwich type@labeled, label free@nonlabeled and aptamer-based electrochemical immunosensor.

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