scholarly journals Cytokine and Cancer Biomarkers Detection: The Dawn of Electrochemical Paper-Based Biosensor

Sensors ◽  
2020 ◽  
Vol 20 (7) ◽  
pp. 1854 ◽  
Author(s):  
Song Wei Loo ◽  
Tze-Sian Pui
Keyword(s):  
Point Of Care ◽  
Cancer Biomarkers ◽  
Healthcare Services ◽  
Vital Role ◽  
Qualitative Assessment ◽  
Detection Methods ◽  
Societal Implications ◽  
Factors Affecting ◽  
Point Of Care Diagnostics ◽  
Sensing Platforms

Although the established ELISA-based sensing platforms have many benefits, the importance of cytokine and cancer biomarkers detection for point-of-care diagnostics has propelled the search for more specific, sensitive, simple, accessible, yet economical sensor. Paper-based biosensor holds promise for future in-situ applications and can provide rapid analysis and data without the need to conduct in a laboratory. Electrochemical detection plays a vital role in interpreting results obtained from qualitative assessment to quantitative determination. In this review, various factors affecting the design of an electrochemical paper-based biosensor are highlighted and discussed in depth. Different detection methods, along with the latest development in utilizing them in cytokine and cancer biomarkers detection, are reviewed. Lastly, the fabrication of portable electrochemical paper-based biosensor is ideal in deliberating positive societal implications in developing countries with limited resources and accessibility to healthcare services.

scholarly journals Current Nucleic Acid Extraction Methods and Their Implications to Point-of-Care Diagnostics

2017 ◽  
Vol 2017 ◽  
pp. 1-13 ◽  
Author(s):  
Nasir Ali ◽  
Rita de Cássia Pontello Rampazzo ◽  
Alexandre Dias Tavares Costa ◽  
Marco Aurelio Krieger
Keyword(s):  
Nucleic Acid ◽  
Extraction Method ◽  
Point Of Care ◽  
Extraction Methods ◽  
Vital Role ◽  
Acid Extraction ◽  
Nucleic Acid Extraction ◽  
Advantages And Disadvantages ◽  
Point Of Care Diagnostics ◽  
Working Principle

Nucleic acid extraction (NAE) plays a vital role in molecular biology as the primary step for many downstream applications. Many modifications have been introduced to the original 1869 method. Modern processes are categorized into chemical or mechanical, each with peculiarities that influence their use, especially in point-of-care diagnostics (POC-Dx). POC-Dx is a new approach aiming to replace sophisticated analytical machinery with microanalytical systems, able to be used near the patient, at thepoint of careorpoint of need. Although notable efforts have been made, a simple and effective extraction method is still a major challenge for widespread use of POC-Dx. In this review, we dissected the working principle of each of the most common NAE methods, overviewing their advantages and disadvantages, as well their potential for integration in POC-Dx systems. At present, it seems difficult, if not impossible, to establish a procedure which can be universally applied to POC-Dx. We also discuss the effects of the NAE chemicals upon the main plastic polymers used to mass produce POC-Dx systems. We end our review discussing the limitations and challenges that should guide the quest for an efficient extraction method that can be integrated in a POC-Dx system.

scholarly journals CRISPR-Based COVID-19 Testing: Toward Next-Generation Point-of-Care Diagnostics

2021 ◽  
Vol 11 ◽  
Author(s):  
Uyanga Ganbaatar ◽  
Changchun Liu
Keyword(s):  
Nucleic Acid ◽  
Point Of Care ◽  
High Sensitivity ◽  
Detection Methods ◽  
Molecular Testing ◽  
Practical Applications ◽  
Food Industries ◽  
Resource Limited ◽  
Point Of Care Diagnostics ◽  
Agriculture And Food

As the COVID-19 pandemic continues, people are becoming infected at an alarming rate, individuals are unknowingly spreading disease, and more lives are lost every day. There is an immediate need for a simple, rapid, early and sensitive point-of-care testing for COVID-19 disease. However, current testing approaches do not meet such need. Recently, clustered regularly interspaced short palindromic repeats (CRISPR)-based detection methods have received substantial attention for nucleic acid-based molecular testing due to their simplicity, high sensitivity and specificity. This review explores the various CRISPR-based COVID-19 detection methods and related diagnostic devices. As with any emerging technology, CRISPR/Cas-based nucleic acid testing methods have several challenges that must be overcome for practical applications in clinics and hospitals. More importantly, these detection methods are not limited to COVID-19 but can be applied to detect any type of pathogen, virus, and fungi that may threaten humans, agriculture, and food industries in resource-limited settings. CRISPR/Cas-based detection methods have the potential to become simpler, more reliable, more affordable, and faster in the near future, which is highly important for achieving point-of-care diagnostics.

scholarly journals Key Enabling Technologies for Point-of-Care Diagnostics

Sensors ◽  
2018 ◽  
Vol 18 (11) ◽  
pp. 3607 ◽  
Author(s):  
Elisabetta Primiceri ◽  
Maria Chiriacò ◽  
Francesca Notarangelo ◽  
Antonio Crocamo ◽  
Diego Ardissino ◽  
...  
Keyword(s):  
Point Of Care ◽  
Low Cost ◽  
Detection Methods ◽  
Common People ◽  
Everyday Objects ◽  
Enabling Technologies ◽  
Point Of Care Diagnostics ◽  
Major Trend ◽  
Key Enabling Technologies ◽  
Highly Sensitive Detection

A major trend in biomedical engineering is the development of reliable, self-contained point-of-care (POC) devices for diagnostics and in-field assays. The new generation of such platforms increasingly addresses the clinical and environmental needs. Moreover, they are becoming more and more integrated with everyday objects, such as smartphones, and their spread among unskilled common people, has the power to improve the quality of life, both in the developed world and in low-resource settings. The future success of these tools will depend on the integration of the relevant key enabling technologies on an industrial scale (microfluidics with microelectronics, highly sensitive detection methods and low-cost materials for easy-to-use tools). Here, recent advances and perspectives will be reviewed across the large spectrum of their applications.

RESTRICTIONS OF EXTENSIVE PRODUCTION TO ENSURE FOOD SECURITY IN CHINA

2020 ◽  
Author(s):  
L. Li ◽  
Keyword(s):  
Food Security ◽  
Food Production ◽  
Agricultural Development ◽  
Science And Technology ◽  
Vital Role ◽  
Food Insufficiency ◽  
Factors Affecting ◽  
Agricultural Mechanization

Being over-dependent on imports, China has been faced with the problem of food insufficiency in recent years.This paper, with the adoption of the indicators of agricultural development and relevant models, aims to explore factors affecting food security in China, in particular, technological elements. The findings demonstrate that technology plays a vital role in improving food production. It is recommended to increase the input of science and technology and improve agricultural mechanization.

Factors Influencing Utilisation of Maternal Healthcare Services: Womens Perception

2015 ◽  
Vol 4 (1and2) ◽  
Author(s):  
Gyanvati
Keyword(s):  
Healthcare Services ◽  
Maternal Healthcare ◽  
Factors Affecting ◽  
Factors Influencing ◽  
Economic Background ◽  
Newborn Child

This paper analyses the factors affecting for utilization of maternal healthcare services. Following is the section wise description of the paper. Firstly, the paper looks into socio-economic background of women. Secondly, the paper focuses on usage of maternal healthcare services by women. Thirdly, the paper also traces the factors affecting for usage of maternal healthcare services. Lastly, the paper concludes and suggests by emphasizing the significance of proper utilization of maternal healthcare services that it leads to better health of mother and their newborn child.

scholarly journals COVID-19 Point-of-Care Diagnostics: Present and Future

ACS Nano ◽  
2021 ◽  
Author(s):  
Enrique Valera ◽  
Aaron Jankelow ◽  
Jongwon Lim ◽  
Victoria Kindratenko ◽  
Anurup Ganguli ◽  
...  
Keyword(s):  
Point Of Care ◽  
Point Of Care Diagnostics

scholarly journals 145 Point of care lung ultrasound in patient triage: integration of ultrasound into a streaming pathway for COVID-19

2020 ◽  
Vol 37 (12) ◽  
pp. 839.1-839
Author(s):  
Dominic Craver ◽  
Aminah Ahmad ◽  
Anna Colclough
Keyword(s):  
Point Of Care ◽  
District General Hospital ◽  
Training Programme ◽  
Service Evaluation ◽  
University Hospital ◽  
Junior Doctors ◽  
Point Of Care Ultrasound ◽  
Face To Face ◽  
Point Of Care Diagnostics ◽  
Audit Data

Aims/Objectives/BackgroundRapid risk stratification of patients is vital for Emergency Department (ED) streaming during the COVID-19 pandemic. Ideally, patients should be split into red (suspected/confirmed COVID-19) and green (non COVID-19) zones in order to minimise the risk of patient-to-patient and patient-to-staff transmission. A robust yet rapid streaming system combining clinician impression with point-of-care diagnostics is therefore necessary.Point of care ultrasound (POCUS) findings in COVID-19 have been shown to correlate well with computed tomography (CT) findings, and it therefore has value as a front-door diagnostic tool. At University Hospital Lewisham (a district general hospital in south London), we recognised the value of early POCUS and its potential for use in patient streaming.Methods/DesignWe developed a training programme, ‘POCUS for COVID’ and subsequently integrated POCUS into streaming of our ED patients. The training involved Zoom lectures, a face to face practical, a 10 scan sign off process followed by a final triggered assessment. Patient outcomes were reviewed in conjunction with their scan reports.Results/ConclusionsCurrently, we have 21 ED junior doctors performing ultrasound scans independently, and all patients presenting to our department are scanned either in triage or in the ambulance. A combination of clinical judgement and scan findings are used to stream the patient to an appropriate area.Service evaluation with analysis of audit data has found our streaming to be 94% sensitive and 79% specific as an indicator of COVID 19. Further analysis is ongoing.Here we present both the structure of our training programme and our integrated streaming pathway along with preliminary analysis results.

scholarly journals Rapid and Sensitive Detection of miRNA Based on AC Electrokinetic Capacitive Sensing for Point-of-Care Applications

Sensors ◽  
2021 ◽  
Vol 21 (12) ◽  
pp. 3985
Author(s):  
Nan Wan ◽  
Yu Jiang ◽  
Jiamei Huang ◽  
Rania Oueslati ◽  
Shigetoshi Eda ◽  
...  
Keyword(s):  
Limit Of Detection ◽  
Point Of Care ◽  
Low Cost ◽  
Clinical Diagnostics ◽  
Detection Methods ◽  
Capacitive Sensing ◽  
Application Potential ◽  
Mirna Detection ◽  
Mirna 25 ◽  
Low Sensitivity

A sensitive and efficient method for microRNAs (miRNAs) detection is strongly desired by clinicians and, in recent years, the search for such a method has drawn much attention. There has been significant interest in using miRNA as biomarkers for multiple diseases and conditions in clinical diagnostics. Presently, most miRNA detection methods suffer from drawbacks, e.g., low sensitivity, long assay time, expensive equipment, trained personnel, or unsuitability for point-of-care. New methodologies are needed to overcome these limitations to allow rapid, sensitive, low-cost, easy-to-use, and portable methods for miRNA detection at the point of care. In this work, to overcome these shortcomings, we integrated capacitive sensing and alternating current electrokinetic effects to detect specific miRNA-16b molecules, as a model, with the limit of detection reaching 1.0 femto molar (fM) levels. The specificity of the sensor was verified by testing miRNA-25, which has the same length as miRNA-16b. The sensor we developed demonstrated significant improvements in sensitivity, response time and cost over other miRNA detection methods, and has application potential at point-of-care.

scholarly journals Point of Care Diagnostics in the Age of COVID-19

Diagnostics ◽  
2020 ◽  
Vol 11 (1) ◽  
pp. 9
Author(s):  
Meysam Rezaei ◽  
Sajad Razavi Bazaz ◽  
Sareh Zhand ◽  
Nima Sayyadi ◽  
Dayong Jin ◽  
...  
Keyword(s):  
Point Of Care ◽  
Diagnostic Methods ◽  
Global Public Health ◽  
Current Standard ◽  
Healthcare Facilities ◽  
Major Threat ◽  
Point Of Care Diagnostics ◽  
Recent Outbreak ◽  
Control Virus ◽  
Biosafety Level

The recent outbreak of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and its associated serious respiratory disease, coronavirus disease 2019 (COVID-19), poses a major threat to global public health. Owing to the lack of vaccine and effective treatments, many countries have been overwhelmed with an exponential spread of the virus and surge in the number of confirmed COVID-19 cases. Current standard diagnostic methods are inadequate for widespread testing as they suffer from prolonged turn-around times (>12 h) and mostly rely on high-biosafety-level laboratories and well-trained technicians. Point-of-care (POC) tests have the potential to vastly improve healthcare in several ways, ranging from enabling earlier detection and easier monitoring of disease to reaching remote populations. In recent years, the field of POC diagnostics has improved markedly with the advent of micro- and nanotechnologies. Due to the COVID-19 pandemic, POC technologies have been rapidly innovated to address key limitations faced in existing standard diagnostic methods. This review summarizes and compares the latest available POC immunoassay, nucleic acid-based and clustered regularly interspaced short palindromic repeats- (CRISPR)-mediated tests for SARS-CoV-2 detection that we anticipate aiding healthcare facilities to control virus infection and prevent subsequent spread.

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