scholarly journals Optical Thickness Monitoring as a Strategic Element for the Development of SPR Sensing Applications

Sensors ◽  
2020 ◽  
Vol 20 (7) ◽  
pp. 1807
Author(s):  
Donato Luna-Moreno ◽  
Araceli Sánchez-Álvarez ◽  
Melissa Rodríguez-Delgado
Keyword(s):  
Gold Film ◽  
Glass Slide ◽  
Response Curves ◽  
Thin Film Sensors ◽  
Rotation System ◽  
Sensing Applications ◽  
Glass Slides ◽  
Resonance Angle ◽  
Au Thin Films ◽  
Strategic Element

The importance of the monitoring of thickness and rate deposition is indispensable for the fabrication of thin film sensors, such as SPR sensors. The sensitivity of SPR responses varies with the thickness of the film, as well as the linear range. Thus, in the present work, we presented an experimental study of the plasmonic response of Cr/Au thin films deposited onto glass slides by evaporation, based on both a rotation and no-rotation system. The results show that the thickness of the gold film varies from 240 to 620 Å, depending on the glass slide position. The SPR response curves obtained experimentally were compared with simulated plasmonic responses and different parameters such as resonance angle, and the depth, slope and half-width of the SPR curve were analysed.

scholarly journals Direct-Plate Serological Grouping of Beta-Hemolytic Streptococci from Primary Isolation Plates with the Phadebact Streptococcus Test

1978 ◽  
Vol 7 (4) ◽  
pp. 356-360
Author(s):  
Malcolm Slifkin ◽  
Carol Engwall ◽  
Gail R. Pouchet
Keyword(s):  
Tween 80 ◽  
Glass Slide ◽  
Correct Identification ◽  
Primary Isolation ◽  
Direct Testing ◽  
Clinical Microbiologist ◽  
Glass Slides ◽  
Precipitin Test

The grouping of beta-hemolytic streptococcal isolates by a new direct-plate procedure employing Phadebact Streptococcus Test reagents was compared with the results obtained with the 4- and 24-h Phadebact grouping procedure and with the Lancefield grouping obtained with a capillary precipitin test. The new procedure employed a modification of the Phadebact procedure that permitted the grouping of streptococci on glass slides with a minimum of five primary isolated colonies. When only five to eight colonies were available for direct testing with each Phadebact reagent, coagglutination was better manifested when the colonies were disaggregated on a glass slide in a loopful of Tween 80 solution. Further enhancement of the coagglutination reaction was effected when the respective Phadebact reagents were employed in relatively small volumes. The direct-plate procedure permitted the correct identification of 127 out of 129 betahemolytic isolates. The 4-h method correctly identified 192 of the 200 streptococci tested. All of the 200 isolates tested by the 24-h procedure and the Lancefield grouping were correctly identified. The direct-plate Phadebact procedure affords the clinical microbiologist a rapid and reliable means of identifying groups A, B, C, and G beta-hemolytic streptococci. When sufficient numbers of primary colonies are not available for the direct procedure, the 4- or 24-h procedures may be employed.

scholarly journals Validation of grading of non-invasive urothelial carcinoma by digital pathology for routine diagnosis

BMC Cancer ◽  
2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Richard Colling ◽  
Hayleigh Colling ◽  
Lisa Browning ◽  
Clare Verrill
Keyword(s):  
Urothelial Carcinoma ◽  
Digital Pathology ◽  
Glass Slide ◽  
Low Grade ◽  
Non Invasive ◽  
Routine Diagnosis ◽  
Grade Versus ◽  
Glass Slides ◽  
Urothelial Bladder ◽  
Bladder Carcinomas

Abstract Background Pathological grading of non-invasive urothelial carcinoma has a direct impact upon management. This study evaluates the reproducibility of grading these tumours on glass slides and digital pathology. Methods Forty eight non-invasive urothelial bladder carcinomas were graded by three uropathologists on glass and on a digital platform using the 1973 WHO and 2004 ISUP/WHO systems. Results Consensus grades for glass and digital grading gave Cohen’s kappa scores of 0.78 (2004) and 0.82 (1973). Of 142 decisions made on the key therapeutic borderline of low grade versus high grade urothelial carcinoma (2004) by the three pathologists, 85% were in agreement. For the 1973 grading system, agreement overall was 90%. Conclusions Agreement on grading on glass slide and digital screen assessment is similar or in some cases improved, suggesting at least non-inferiority of DP for grading of non-invasive urothelial carcinoma.

scholarly journals High-sensitivity SERS based sensing on the labeling side of glass slides using low branched gold nanoparticles prepared with surfactant-free synthesis

RSC Advances ◽  
2020 ◽  
Vol 10 (56) ◽  
pp. 34290-34298
Author(s):  
Tuğba Tezcan ◽  
Chia-Hsien Hsu
Keyword(s):  
Gold Nanoparticles ◽  
High Sensitivity ◽  
Glass Slide ◽  
Dopamine Detection ◽  
Glass Slides

High-sensitivity dopamine detection on aggregated low branched nanoparticles on labelling side of glass slide as a SERS based sensor.

scholarly journals Gold/Polyimide-Based Resistive Strain Sensors

Electronics ◽  
2019 ◽  
Vol 8 (5) ◽  
pp. 565 ◽  
Author(s):  
Tao Han ◽  
Anindya Nag ◽  
Nasrin Afsarimanesh ◽  
Fowzia Akhter ◽  
Hangrui Liu ◽  
...  
Keyword(s):  
Thin Film ◽  
Industrial Applications ◽  
Gold Layer ◽  
Gauge Factor ◽  
Strain Sensing ◽  
Thin Film Sensors ◽  
Pressure Mapping ◽  
Sensing Applications ◽  
The Cost ◽  
Two Sides

This paper presents the fabrication and implementation of novel resistive sensors that were implemented for strain-sensing applications. Some of the critical factors for the development of resistive sensors are addressed in this paper, such as the cost of fabrication, the steps of the fabrication process which make it time-consuming to complete each prototype, and the inability to achieve optimised electrical and mechanical characteristics. The sensors were fabricated via magnetron sputtering of thin-film chromium and gold layer on the thin-film substrates at defined thicknesses. Sticky copper tapes were attached on the two sides of the sensor patches to form the electrodes. The operating principle of the fabricated sensors was based on the change in their responses with respect to the corresponding changes in their relative resistance as a function of the applied strain. The strain-induced characteristics of the patches were studied with different kinds of experiments, such as consecutive bending and pressure application. The sensors with 400 nm thickness of gold layer obtained a sensitivity of 0.0086 Ω/ppm for the pressure ranging between 0 and 400 kPa. The gauge factor of these sensors was between 4.9–6.6 for temperatures ranging between 25 °C and 55 °C. They were also used for tactile sensing to determine their potential as thin-film sensors for industrial applications, like in robotic and pressure-mapping applications. The results were promising in regards to the sensors’ controllable film thickness, easy operation, purity of the films and mechanically sound nature. These sensors can provide a podium to enhance the usage of resistive sensors on a higher scale to develop thin-film sensors for industrial applications.

Hints On Removing Epon Specimen Blocks From Glass Slides

1998 ◽  
Vol 6 (3) ◽  
pp. 14-17
Author(s):  
Wolfgang Muss
Keyword(s):  
Physical Properties ◽  
Thin Section ◽  
Glass Slide ◽  
Glass Slides

Depending on the quality of the glass slide (not all brands seem to be the same quality and therefore display variable physical properties), post-polymerized specimen blocks can be separated from object slides. These can be of varying area size, and can include a selected area of your former semi-thin section or the whole area (as used in re-embedding techniques).

scholarly journals Virtual microscopy in cytotechnology education: Application of knowledge from virtual to glass

CytoJournal ◽  
2012 ◽  
Vol 9 ◽  
pp. 12 ◽  
Author(s):  
Amber D Donnelly ◽  
Maheswari S Mukherjee ◽  
Elizabeth R Lyden ◽  
Stanley J Radio
Keyword(s):  
Test Scores ◽  
Screening Test ◽  
Online Survey ◽  
Digital Images ◽  
Virtual Microscopy ◽  
Needle Aspiration ◽  
Glass Slide ◽  
Cellular Morphology ◽  
Teaching Tool ◽  
Glass Slides

Background:Virtual microscopy (VM) is a technology in which the glass slides are converted into digital images. The main objective of this study is to determine if cellular morphology, learned through virtual microscopy, can be applied to glass slide screening.Materials and Methods:A total of 142 glass slides (61 teaching and 81 practice) of breast, thyroid, and lymph node fine needle aspiration body sites were scanned with a single focal plane (at 40X) using iScanCoreo Au (Ventana, Tuscan, AZ, USA, formerly known as BioImagene, California, USA). Six students including one distant student used these digital images to learn cellular morphology and conduct daily screening. Subsequently, all the students were tested on 10 glass slides using light microscopy (LM). At the end of the study, the students were asked to respond to an online survey on their virtual microscopy experience. The glass slide screening test scores of the participating students who were taught through VM and tested on glass slides (VMLM group) were compared with the last three classes of students who were taught through LM and tested on glass slides (LMLM group).Results:A non-parametric statistical analysis indicated no difference (P= 0.20) in the glass screening test scores between VMLM (median = 93.5) and LMLM groups (median = 87). The survey indicated that the annotated teaching slides and access to the VM, off campus, were well appreciated by the students.Conclusions:Although the students preferred LM, they were able to apply the cytological criteria learned through VM to glass slide screening. Overall, VM was considered a great teaching tool.

Concordance between digital pathology and light microscopy in general surgical pathology: a pilot study of 100 cases

2014 ◽  
Vol 67 (12) ◽  
pp. 1052-1055 ◽  
Author(s):  
Joseph P Houghton ◽  
Aaron J Ervine ◽  
Sarah L Kenny ◽  
Paul J Kelly ◽  
Seamus S Napier ◽  
...  
Keyword(s):  
Pilot Study ◽  
Light Microscopy ◽  
Digital Images ◽  
Digital Pathology ◽  
Glass Slide ◽  
Clinical Consequence ◽  
Histopathological Diagnosis ◽  
Original Diagnosis ◽  
Glass Slides ◽  
Viable Method

Aim(1) A pilot study to determine the accuracy of interpretation of whole slide digital images in a broad range of general histopathology cases of graded complexity. (2) To survey the participating histopathologists with regard to acceptability of digital pathology.Materials and methodsGlass slides of 100 biopsies and minor resections were digitally scanned in their entirety, producing digital slides. These cases had been diagnosed by light microscopy at least 1 year previously and were subsequently reassessed by the original reporting pathologist (who was blinded to their original diagnosis) using digital pathology. The digital pathology-based diagnosis was compared with the original glass slide diagnosis and classified as concordant, slightly discordant (without clinical consequence) or discordant. The participants were surveyed at the end of the study.ResultsThere was concordance between the original light microscopy diagnosis and digital pathology-based diagnosis in 95 of the 100 cases while the remaining 5 cases showed only slight discordance (with no clinical consequence). None of the cases were categorised as discordant. Participants had mixed experiences using digital pathology technology.ConclusionsIn the broad range of cases we examined, digital pathology is a safe and viable method of making a primary histopathological diagnosis.

Influence of the Metal Film Thickness on the Sensitivity of Surface Plasmon Resonance Biosensors

2005 ◽  
Vol 59 (5) ◽  
pp. 661-667 ◽  
Author(s):  
Sanong Ekgasit ◽  
Chuchaat Thammacharoen ◽  
Fang Yu ◽  
Wolfgang Knoll
Keyword(s):  
Surface Plasmon Resonance ◽  
Film Thickness ◽  
Surface Plasmon ◽  
Metal Film ◽  
Gold Film ◽  
Metal Films ◽  
Thickness Variation ◽  
Reflectance Change ◽  
Angle Shift ◽  
Resonance Angle

The influence of the metal film thickness (i.e., the chromium adhesion promoting film and the gold film) on the sensitivity of surface plasmon resonance (SPR) signals (i.e., resonance angle shift and reflectance change) towards the thickness variation of the nonabsorbing dielectric film is investigated. The sensitivity of reflectance change decreases when a thick chromium film or a thin gold film is employed. Its linear range becomes narrower as the thickness of the metal films increases. The sensitivity and linear range of the resonance angle shift are not affected by the thickness variation of the metal films. The phenomena were theoretically explained based on the attenuated total reflection (ATR) generated evanescent field at the prism/metal interface and the SPR-generated evanescent field at the metal/dielectric interface.

Competition for endogenous and exogenous nutrients between Sporobolomyces roseus and Cochliobolus sativus

1984 ◽  
Vol 62 (11) ◽  
pp. 2463-2468 ◽  
Author(s):  
N. J. Fokkema
Keyword(s):  
Germ Tube ◽  
Conidial Germination ◽  
Glass Slide ◽  
Cochliobolus Sativus ◽  
Tube Length ◽  
Glass Slides ◽  
Germ Tube Length ◽  
Cellophane Sheet ◽  
Sporobolomyces Roseus ◽  
Exogenous Nutrients

Sporobolomyces roseus reduced conidial germination of Cochliobolus sativus on glass slides covered with a thin layer (0.1 mm) of water agar or Czapek Dox agar by ca. 60 and 99%, respectively. On wheat flag leaves S. roseus reduced conidial germination by ca. 15% but reduced germ tube length by ca. 48%. However, cell-free aqueous diffusates collected from water agar slides and leaves with and without S. roseus showed no differential effect on Co. sativus when bioassayed on water agar slides. Diffusates from Czapek Dox agar with S. roseus reduced conidial germination by 46% when compared with the nutrient-rich diffusate from Czapek Dox agar alone. Although this demonstrated the ability of the yeasts to reduce the amount of exogenous nutrients, this did not account for the 99% reduction found in the presence of S. roseus. When Co. sativus was separated from S. roseus on Czapek Dox agar by two layers of cellophane, the reduction was the same and maintained for at least 3 days. If Co. sativus was removed from S. roseus by transferring the upper cellophane sheet to a glass slide, germination was restored, indicating that the yeasts formed also a continuous drain of endogenous nutrients from the conidia. A steady supply of amino acids and (or) glucose to Czapek Dox agar slides with S. roseus and Co. sativus could only partially overcome the antagonistic interaction.

scholarly journals Simulation/Experiment Confrontation, an Efficient Approach for Sensitive SAW Sensors Design

Sensors ◽  
2020 ◽  
Vol 20 (17) ◽  
pp. 4994
Author(s):  
Bilel Achour ◽  
Ghada Attia ◽  
Chouki Zerrouki ◽  
Najla Fourati ◽  
Kosai Raoof ◽  
...  
Keyword(s):  
Transfer Functions ◽  
Dissociation Constants ◽  
Limit Of Detection ◽  
Fem Simulation ◽  
Response Curves ◽  
Time Saving ◽  
Fourfold Increase ◽  
Sensing Applications ◽  
Kd Values ◽  
Good Agreement

Sensitivity is one of the most important parameters to put in the foreground in all sensing applications. Its increase is therefore an ongoing challenge, particularly for surface acoustic wave (SAW) sensors. Herein, finite element method (FEM) simulation using COMSOL Multiphysics software is first used to simulate the physical and electrical properties of SAW delay line. Results indicate that 2D configuration permits to accurately obtain all pertinent parameters, as in 3D simulation, with very substantial time saving. A good agreement between calculation and experiment, in terms of transfer functions (S21 spectra), was also shown to evaluate the dependence of the SAW sensors sensitivity on the operating frequency; 2D simulations have been conducted on 104 MHz and 208 MHz delay lines, coated with a polyisobutylene (PIB) as sensitive layer to dichloromethane (DCM). A fourfold increase in sensitivity was obtained by doubling frequency. Both sensors were then realized and tested as chem-sensors to detect zinc ions in liquid media. 9-{[4-({[4-(9anthrylmethoxy)phenyl]sulfanyl} methyl)]methyl] anthracene (TDP-AN) was selected as the sensing layer. Results show a comparable response curves for both designed sensors, in terms of limit of detection and dissociation constants Kd values. On the other hand, experimental sensitivity values were of the order of [7.0 ± 2.8] × 108 [°/M] and [16.0 ± 7.6] × 108 [°/M] for 104 MHz and 208 MHz sensors, respectively, confirming that the sensitivity increases with frequency.

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