scholarly journals A Facile Fabrication of a Potentiometric Arrayed Glucose Biosensor Based on Nafion-GOx/GO/AZO

Sensors ◽  
2020 ◽  
Vol 20 (4) ◽  
pp. 964
Author(s):  
Jung-Chuan Chou ◽  
Si-Hong Lin ◽  
Tsu-Yang Lai ◽  
Po-Yu Kuo ◽  
Chih-Hsien Lai ◽  
...  
Keyword(s):  
Zinc Oxide ◽  
Electrochemical Impedance ◽  
Limit Of Detection ◽  
Low Cost ◽  
Glucose Biosensor ◽  
Average Sensitivity ◽  
Glucose Biosensors ◽  
Coating Method ◽  
Aluminum Doped Zinc Oxide ◽  
Screen Printing Technology

In this study, the potentiometric arrayed glucose biosensors, which were based on zinc oxide (ZnO) or aluminum-doped zinc oxide (AZO) sensing membranes, were fabricated by using screen-printing technology and a sputtering system, and graphene oxide (GO) and Nafion-glucose oxidase (GOx) were used to modify sensing membranes by using the drop-coating method. Next, the material properties were characterized by using a Raman spectrometer, a field-emission scanning electron microscope (FE-SEM), and a scanning probe microscope (SPM). The sensing characteristics of the glucose biosensors were measured by using the voltage–time (V-T) measurement system. Finally, electrochemical impedance spectroscopy (EIS) was conducted to analyze their charge transfer abilities. The results indicated that the average sensitivity of the glucose biosensor based on Nafion-GOx/GO/AZO was apparently higher than that of the glucose biosensor based on Nafion-GOx/GO/ZnO. In addition, the glucose biosensor based on Nafion-GOx/GO/AZO exhibited an excellent average sensitivity of 15.44 mV/mM and linearity of 0.997 over a narrow range of glucose concentration range, a response time of 26 s, a limit of detection (LOD) of 1.89 mM, and good reproducibility. In terms of the reversibility and stability, the hysteresis voltages (VH) were 3.96 mV and 2.42 mV. Additionally, the glucose biosensor also showed good anti-inference ability and reproducibility. According to these results, it is demonstrated that AZO is a promising material, which could be used to develop a reliable, simple, and low-cost potentiometric glucose biosensor.

scholarly journals Investigation of Flexible Arrayed Lactate Biosensor Based on Copper Doped Zinc Oxide Films Modified by Iron–Platinum Nanoparticles

Polymers ◽  
2021 ◽  
Vol 13 (13) ◽  
pp. 2062
Author(s):  
Yu-Hsun Nien ◽  
Zhi-Xuan Kang ◽  
Tzu-Yu Su ◽  
Chih-Sung Ho ◽  
Jung-Chuan Chou ◽  
...  
Keyword(s):  
Zinc Oxide ◽  
Response Time ◽  
Platinum Nanoparticles ◽  
Electrochemical Impedance ◽  
Rf Sputtering ◽  
X Ray Diffraction ◽  
Average Sensitivity ◽  
Iron Platinum ◽  
Zinc Oxide Films ◽  
Paste Electrode

Potentiometric biosensors based on flexible arrayed silver paste electrode and copper-doped zinc oxide sensing film modified by iron-platinum nanoparticles (FePt NPs) are designed and manufactured to detect lactate in human. The sensing film is made of copper-doped zinc oxide (CZO) by a radio frequency (RF) sputtering system, and then modified by iron-platinum nanoparticles (FePt NPs). The surface morphology of copper-doped zinc oxide (CZO) is analyzed by scanning electron microscope (SEM). FePt NPs are analyzed by X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR). The average sensitivity, response time, and interference effect of the lactate biosensors are analyzed by voltage-time (V-T) measurement system. The electrochemical impedance is analyzed by electrochemical impedance spectroscopy (EIS). The average sensitivity and linearity over the concentration range 0.2 mM–5 mM are 25.32 mV/mM and 0.977 mV/mM, respectively. The response time of the lactate biosensor is 16 s, with excellent selectivity.

scholarly journals Near-perfect broadband absorption from hyperbolic metamaterial nanoparticles

2017 ◽  
Vol 114 (6) ◽  
pp. 1264-1268 ◽  
Author(s):  
Conor T. Riley ◽  
Joseph S. T. Smalley ◽  
Jeffrey R. J. Brodie ◽  
Yeshaiahu Fainman ◽  
Donald J. Sirbuly ◽  
...  
Keyword(s):  
Zinc Oxide ◽  
High Performance ◽  
Low Cost ◽  
Perfect Absorption ◽  
Hyperbolic Metamaterial ◽  
Broadband Absorption ◽  
Essential Components ◽  
Aluminum Doped Zinc Oxide ◽  
Detection Energy ◽  
Planar Films

Broadband absorbers are essential components of many light detection, energy harvesting, and camouflage schemes. Current designs are either bulky or use planar films that cause problems in cracking and delamination during flexing or heating. In addition, transferring planar materials to flexible, thin, or low-cost substrates poses a significant challenge. On the other hand, particle-based materials are highly flexible and can be transferred and assembled onto a more desirable substrate but have not shown high performance as an absorber in a standalone system. Here, we introduce a class of particle absorbers called transferable hyperbolic metamaterial particles (THMMP) that display selective, omnidirectional, tunable, broadband absorption when closely packed. This is demonstrated with vertically aligned hyperbolic nanotube (HNT) arrays composed of alternating layers of aluminum-doped zinc oxide and zinc oxide. The broadband absorption measures >87% from 1,200 nm to over 2,200 nm with a maximum absorption of 98.1% at 1,550 nm and remains large for high angles. Furthermore, we show the advantages of particle-based absorbers by transferring the HNTs to a polymer substrate that shows excellent mechanical flexibility and visible transparency while maintaining near-perfect absorption in the telecommunications region. In addition, other material systems and geometries are proposed for a wider range of applications.

scholarly journals Electrochemical micro analytical device interfaced with portable potentiostat for rapid detection of chlorpyrifos using acetylcholinesterase conjugated metal organic framework using Internet of things

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Shalini Nagabooshanam ◽  
Souradeep Roy ◽  
Ashish Mathur ◽  
Irani Mukherjee ◽  
Satheesh Krishnamurthy ◽  
...  
Keyword(s):  
Electrochemical Impedance ◽  
Limit Of Detection ◽  
Low Cost ◽  
Metal Organic Framework ◽  
Electrochemical Techniques ◽  
Electrochemical Analysis ◽  
Vegetable Sample ◽  
Metal Organic ◽  
Analytical Device ◽  
Organic Framework

AbstractAn Electrochemical micro Analytical Device (EµAD) was fabricated for sensitive detection of organophosphate pesticide chlorpyrifos in the food chain. Gold microelectrode (µE) modified with Zinc based Metal Organic Framework (MOF-Basolite Z1200) and Acetylcholinesterase (AChE) enzyme served as an excellent electro-analytical transducer for the detection of chlorpyrifos. Electrochemical techniques such as Cyclic Voltammetry (CV), Electrochemical Impedance Spectroscopy (EIS) and Differential Pulse Voltammetry (DPV) were performed for electrochemical analysis of the developed EµAD. The sensor needs only 2 µL of the analyte and it was tested within the linear range of 10 to 100 ng/L. The developed EµAD’s limit of detection (LoD) and sensitivity is 6 ng/L and 0.598 µ A/ng L−1/mm2 respectively. The applicability of the device for the detection of chlorpyrifos from the real vegetable sample was also tested within the range specified. The fabricated sensor showed good stability with a shelf-life of 20 days. The EµAD’s response time is of 50 s, including an incubation time of 20 s. The developed EµAD was also integrated with commercially available low-cost, handheld potentiostat (k-Stat) using Bluetooth and the results were comparable with a standard electrochemical workstation.

scholarly journals Paracetamol Sensing with a Pencil Lead Electrode Modified with Carbon Nanotubes and Polyvinylpyrrolidone

Chemosensors ◽  
2020 ◽  
Vol 8 (4) ◽  
pp. 133
Author(s):  
Piyanut Pinyou ◽  
Vincent Blay ◽  
Kantapat Chansaenpak ◽  
Sireerat Lisnund
Keyword(s):  
Carbon Nanotubes ◽  
Electrochemical Impedance ◽  
Limit Of Detection ◽  
Low Cost ◽  
Electrical Contact ◽  
Pharmaceutical Preparations ◽  
Single Wall Carbon Nanotubes ◽  
Wave Voltammetry ◽  
Lead Electrode

The determination of paracetamol is a common need in pharmaceutical and environmental samples for which a low-cost, rapid, and accurate sensor would be highly desirable. We develop a novel pencil graphite lead electrode (PGE) modified with single-wall carbon nanotubes (SWCNTs) and polyvinylpyrrolidone (PVP) polymer (PVP/SWCNT/PGE) for the voltammetric quantification of paracetamol. The sensor shows remarkable analytical performance in the determination of paracetamol at neutral pH, with a limit of detection of 0.38 μM and a linear response from 1 to 500 μM using square-wave voltammetry (SWV), which are well suited to the analysis of pharmaceutical preparations. The introduction of the polymer PVP can cause dramatic changes in the sensing performance of the electrode, depending on its specific architecture. These effects were investigated using cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and scanning electron microscopy (SEM). The results indicate that the co-localization and dispersion of PVP throughout the carbon nanotubes on the electrode are key to its superior electrochemical performance, facilitating the electrical contact between the nanotubes and with the electrode surface. The application of this sensor to commercial syrup and tablet preparations is demonstrated with excellent results.

scholarly journals Synthesis and characterization of a novel non-enzymatic glucose biosensor based on polyaniline/zinc oxide/multi-walled carbon nanotube ternary nanocomposite

2019 ◽  
Vol 9 (3) ◽  
pp. 207-222 ◽  
Author(s):  
Soha Mohajeri ◽  
Abolghassem Dolati ◽  
Khashayar Yazdanbakhsh
Keyword(s):  
Zinc Oxide ◽  
Carbon Nanotube ◽  
Limit Of Detection ◽  
Industrial Applications ◽  
Glucose Biosensor ◽  
Potential Cycling ◽  
Cobalt Sulfate ◽  
X Ray ◽  
Ternary Nanocomposite ◽  
Multi Walled Carbon Nanotube

Novel polyaniline/zinc oxide/multi-walled carbon nanotube (PANI/ZnO/MWCNT) ternary nanocomposite was fabricated as a non-enzymatic glucose biosensor. Thermal chemical vapor deposition (CVD) process was employed to synthesize vertically aligned MWCNTs on stainless steel substrates coated by Co catalyst nanoparticles. In order to fabricate sensitive and reliable MWCNT-based biosensors, nanotubes density and alignment were adjusted by varying the CVD reaction time and cobalt sulfate concentration. The fabricated nanotubes were modified by ZnO particles through the potentiostatic electrodeposition technique. Optimal electrodeposition potential, electrodeposition time, and electrolyte concentration values were determined. The optimized ZnO/MWCNT nanocomposite was reinforced by polyaniline (PANI) nanofibers through the potential cycling technique, and the morphology, elemental composition, and phase structure of the fabricated nanocomposites were characterized by scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDX), and X-ray diffraction (XRD), respectively. The sensing mechanism of the PANI/ZnO/MWCNT electrode for the electrochemical detection of glucose was investigated, and the limit of detection and sensitivity values of the designed sensor were determined. The fast response time of the ternary nanocomposite-based sensor as well as its satisfactory stability and reproducibility, makes it a promising candidate for non-enzymatic detection of glucose in biomedical, environmental, and industrial applications.

Flexible Layer-by-Layer Self-Assembled Graphene Based Glucose Biosensors

2011 ◽  
Author(s):  
Bo Zhang ◽  
Tony Zhengyu Cui
Keyword(s):  
Self Assembly ◽  
Low Cost ◽  
Glucose Biosensor ◽  
Layer By Layer ◽  
Glucose Biosensors ◽  
Flexible Polymer ◽  
Assembly Technique ◽  
Self Assembled ◽  
The Cost

The manufacture and characterization of glucose biosensor based on layer by layer self assembled graphene are presented. Due to self assembly technique and flexible polymer substrate, the cost of the biosensor is very competitive. The resolution of the graphene based biosensor reaches down to 10 pM, which shows greater advantages over CNT based biosensor under the same conditions. The response time of graphene biosensor is less than 3 s, which is much faster than other materials and methods. This work demonstrates that graphene and polymers are very promising materials for the applications of low-cost glucose biosensors.

scholarly journals An amperometric glucose biosensor based on PEDOT nanofibers

RSC Advances ◽  
2018 ◽  
Vol 8 (35) ◽  
pp. 19724-19731 ◽  
Author(s):  
Merih Zeynep Çetin ◽  
Pinar Camurlu
Keyword(s):  
Low Cost ◽  
Chemical Vapor ◽  
Glucose Biosensor ◽  
Glucose Biosensors ◽  
Robust Approach

A low cost, robust approach for the production of glucose biosensors was achieved via simple electrospinning and chemical vapor polymerization techniques.

scholarly journals Label free, electric field mediated ultrasensitive electrochemical point-of-care device for CEA detection

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
B. Chakraborty ◽  
A. Das ◽  
N. Mandal ◽  
N. Samanta ◽  
N. Das ◽  
...  
Keyword(s):  
Electric Field ◽  
Electrochemical Impedance ◽  
Limit Of Detection ◽  
Point Of Care ◽  
Low Cost ◽  
Zno Nanorods ◽  
Hybrid Nanostructures ◽  
Electrochemical Sensing ◽  
Label Free ◽  
Electron Transfer Rate

AbstractDeveloping point-of-care (PoC) diagnostic platforms for carcinoembryonic antigen detection is essential. However, thefew implementations of transferring the signal amplification strategies in electrochemical sensing on paper-based platforms are not satisfactory in terms of detection limit (LOD). In the quest for pushing down LOD, majority of the research has been targeted towards development of improved nanostructured substrates for entrapping more analyte molecules and augmenting the electron transfer rate to the working electrode. But, such approaches have reached saturation. This paper focuses on enhancing the mass transport of the analyte towards the sensor surface through the application of an electric field, in graphene-ZnO nanorods heterostructure. These hybrid nanostructures have been deposited on flexible polyethylene terephthalate substrates with screen printed electrodes for PoC application. The ZnO nanorods have been functionalized with aptamers and the working sensor has been integrated with smartphone interfaced indigenously developed low cost potentiostat. The performance of the system, requiring only 50 µl analyte has been evaluated using electrochemical impedance spectroscopy and validated against commercially available ELISA kit. Limit of detection of 1 fg/ml in human serum with 6.5% coefficient of variation has been demonstrated, which is more than three orders of magnitude lower than the existing attempts on PoC device.

scholarly journals Homocysteine Solution-Induced Response in Aerosol Jet Printed OECTs by Means of Gold and Platinum Gate Electrodes

2021 ◽  
Vol 22 (21) ◽  
pp. 11507
Author(s):  
Pasquale D’Angelo ◽  
Mario Barra ◽  
Patrizia Lombari ◽  
Annapaola Coppola ◽  
Davide Vurro ◽  
...  
Keyword(s):  
High Performance ◽  
Electrochemical Impedance ◽  
Limit Of Detection ◽  
Biological Fluids ◽  
Point Of Care ◽  
Low Cost ◽  
Induced Response ◽  
Gate Electrodes ◽  
Standard Procedures ◽  
Experimental Protocols

Homocysteine (Hcy) is a non-protein, sulfur-containing amino acid, which is recognized as a possible risk factor for coronary artery and other pathologies when its levels in the blood exceed the normal range of between 5 and 12 μmol/L (hyperhomocysteinemia). At present, standard procedures in laboratory medicine, such as high-performance liquid chromatography (HPLC), are commonly employed for the quantitation of total Hcy (tHcy), i.e., the sum of the protein-bound (oxidized) and free (homocystine plus reduced Hcy) forms, in biological fluids (particularly, serum or plasma). Here, the response of Aerosol Jet-printed organic electrochemical transistors (OECTs), in the presence of either reduced (free) and oxidized Hcy-based solutions, was analyzed. Two different experimental protocols were followed to this end: the former consisting of gold (Au) electrodes’ biothiol-induced thiolation, while the latter simply used bare platinum (Pt) electrodes. Electrochemical impedance spectroscopy (EIS) analysis was performed both to validate the gold thiolation protocol and to gain insights into the reduced Hcy sensing mechanism by the Au-gated OECTs, which provided a final limit of detection (LoD) of 80 nM. For the OECT response based on Platinum gate electrodes, on the other hand, a LoD of 180 nM was found in the presence of albumin-bound Hcy, with this being the most abundant oxidized Hcy-form (i.e., the protein-bound form) in physiological fluids. Despite the lack of any biochemical functionalization supporting the response selectivity, the findings discussed in this work highlight the potential role of OECT in the development of low-cost point-of-care (POC) electronic platforms that are suitable for the evaluation, in humans, of Hcy levels within the physiological range and in cases of hyperhomocysteinemia.

scholarly journals A Brief Description of Cyclic Voltammetry Transducer-Based Non-Enzymatic Glucose Biosensor Using Synthesized Graphene Electrodes

2020 ◽  
Vol 3 (3) ◽  
pp. 32
Author(s):  
Mohamed Husien Fahmy Taha ◽  
Hager Ashraf ◽  
Wahyu Caesarendra
Keyword(s):  
Cyclic Voltammetry ◽  
Electrochemical Properties ◽  
High Performance ◽  
Ph Value ◽  
Limit Of Detection ◽  
Low Cost ◽  
Synthesis Method ◽  
Glucose Biosensor ◽  
Glucose Sensors ◽  
Fourth Generation

The essential disadvantages of conventional glucose enzymatic biosensors such as high fabrication cost, poor stability of enzymes, pH value-dependent, and dedicated limitations, have been increasing the attraction of non-enzymatic glucose sensors research. Beneficially, patients with diabetes could use this type of sensor as a fourth-generation of glucose sensors with a very low cost and high performance. We demonstrate the most common acceptable transducer for a non-enzymatic glucose biosensor with a brief description of how it works. The review describes the utilization of graphene and its composites as new materials for high-performance non-enzymatic glucose biosensors. The electrochemical properties of graphene and the electrochemical characterization using the cyclic voltammetry (CV) technique of electrocatalysis electrodes towards glucose oxidation have been summarized. A recent synthesis method of the graphene-based electrodes for non-enzymatic glucose sensors have been introduced along with this study. Finally, the electrochemical properties such as linearity, sensitivity, and the limit of detection (LOD) for each sensor are introduced with a comparison with each other to figure out their strengths and weaknesses.

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