scholarly journals A Low-Cost Micro-Volume Nephelometric System for Quantitative Immunoagglutination Assays

Sensors ◽  
2019 ◽  
Vol 19 (20) ◽  
pp. 4359 ◽  
Author(s):  
Qiqi Sun ◽  
Wei Zheng ◽  
Chao Lin ◽  
Dongxuan Shen
Keyword(s):  
Low Cost ◽  
Protein A ◽  
High Sensitivity ◽  
High Specificity ◽  
Sample Volume ◽  
C Reactive Protein ◽  
Detection Capability ◽  
Reactive Protein ◽  
Detection Volume ◽  
Reagent Cost

Immunoassays have been widely used in scientific research and clinical diagnosis due to their versatile detection capability and high specificity. Immunoagglutination assays are kinds of immunoassay, which can simply and rapidly measure the concentration of analytes. In this work, we developed a low-cost micro-volume nephelometric system for quantitative immunoagglutination assays. We used off-the-shelf components to build the system, and the total cost of key components is only about 20 US dollars. The total detection volume in our system was as low as 3 µL, which could significantly reduce the reagent cost and required sample volume. We further evaluated the system performance via the immunoagglutination assay to measure the concentration of C-reactive protein, a plasma protein with levels rising in response to inflammation. The results demonstrated that our system could measure the concentration of analytes with relatively high sensitivity and precision within four minutes, and has high potential to be applied for clinical diagnostic tests.

scholarly journals Demonstration of a Label-Free and Low-Cost Optical Cavity-Based Biosensor Using Streptavidin and C-Reactive Protein

Biosensors ◽  
2020 ◽  
Vol 11 (1) ◽  
pp. 4
Author(s):  
Donggee Rho ◽  
Seunghyun Kim
Keyword(s):  
Limit Of Detection ◽  
Point Of Care ◽  
Low Cost ◽  
Optical Cavity ◽  
Sample Volume ◽  
Small Sample ◽  
Label Free ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Cavity Structure

An optical cavity-based biosensor (OCB) has been developed for point-of-care (POC) applications. This label-free biosensor employs low-cost components and simple fabrication processes to lower the overall cost while achieving high sensitivity using a differential detection method. To experimentally demonstrate its limit of detection (LOD), we conducted biosensing experiments with streptavidin and C-reactive protein (CRP). The optical cavity structure was optimized further for better sensitivity and easier fluid control. We utilized the polymer swelling property to fine-tune the optical cavity width, which significantly improved the success rate to produce measurable samples. Four different concentrations of streptavidin were tested in triplicate, and the LOD of the OCB was determined to be 1.35 nM. The OCB also successfully detected three different concentrations of human CRP using biotinylated CRP antibody. The LOD for CRP detection was 377 pM. All measurements were done using a small sample volume of 15 µL within 30 min. By reducing the sensing area, improving the functionalization and passivation processes, and increasing the sample volume, the LOD of the OCB are estimated to be reduced further to the femto-molar range. Overall, the demonstrated capability of the OCB in the present work shows great potential to be used as a promising POC biosensor.

High sensitivity C-reactive protein: a predictor for recurrence of atrial fibrillation after successful cardioversion

2009 ◽  
Vol 4 (4) ◽  
pp. 309-313 ◽  
Author(s):  
Enrico Vizzardi ◽  
Savina Nodari ◽  
Gregoriana Zanini ◽  
Alessandra Manerba ◽  
Silvia Frattini ◽  
...  
Keyword(s):  
Atrial Fibrillation ◽  
Protein A ◽  
High Sensitivity ◽  
C Reactive Protein ◽  
Reactive Protein

High-sensitivity C-reactive protein: A predicative marker in severe asthma

Respirology ◽  
2008 ◽  
Vol 13 (5) ◽  
pp. 664-669 ◽  
Author(s):  
Fen-Hong QIAN ◽  
Qian ZHANG ◽  
Lin-Fu ZHOU ◽  
Hua LIU ◽  
Mao HUANG ◽  
...  
Keyword(s):  
Severe Asthma ◽  
Protein A ◽  
High Sensitivity ◽  
C Reactive Protein ◽  
Reactive Protein

scholarly journals Early-Onset Neonatal Sepsis: Role of C-Reactive Protein, Micro-ESR, and Gastric Aspirate for Polymorphs as Screening Markers

2021 ◽  
Vol 2021 ◽  
pp. 1-6
Author(s):  
Sukhdeep Kaur ◽  
Kunwarpal Singh
Keyword(s):  
Neonatal Sepsis ◽  
Early Onset ◽  
Capillary Tube ◽  
High Sensitivity ◽  
High Specificity ◽  
Screening Tests ◽  
Gastric Aspirate ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Sepsis Screen

Introduction. Early-onset neonatal sepsis is a major cause of morbidness and death in newborn children. Its timely diagnosis is usually a challenge in developing countries like India. Aim. To study the efficacy of C-reactive protein (CRP), micro-ESR, and gastric aspirate for polymorphs in the diagnosis of early-onset neonatal sepsis. Materials and Methods. This study included sixty term and preterm children, inborn and referred cases. The children who presented before day seven of life with clinical suspicion of sepsis or who were at high risk of developing sepsis were included. These were further investigated. Significant values for screening tests were taken as C − reactive   protein > 0.6   mg / dl , micro-ESR—after 1 hour, fall in the column of blood in capillary tube was measured, and result was taken as mm fall in 1 hr, and gastric aspirate for polymorphs > 5   polymorphs / HPF . Sepsis screen positive result was 2 or more positive tests. The statistical evaluation was done using Fisher, and ANOVA tests using SPSS 20.0 version. Results. Sixty children were included in the study with forty as the referred ones. Most of them had tachypnea (45%). CRP showed high sensitivity, whereas micro-ESR and gastric aspirate for polymorphs showed high specificity. Conclusions. Neonatal sepsis screening is required for the detection of infection as the blood culture report may not be positive in all the cases, and even if positive, the result takes few hours. CRP showed high sensitivity, whereas micro-ESR and gastric aspirate for polymorphs showed high specificity independently as well as when combined.

scholarly journals Fibrinogen with high-sensitivity C-reactive protein: A combined prediction biomarker for acute coronary syndrome risk assessment

ScienceAsia ◽  
2022 ◽  
Vol 48 (1) ◽  
pp. 8
Author(s):  
Chananikan Makmool ◽  
Burabha Pussadhamma ◽  
Wit Leuangwatthananon ◽  
Songpol Howhan ◽  
Nantarat Komanasin
Keyword(s):  
Risk Assessment ◽  
Acute Coronary Syndrome ◽  
Protein A ◽  
High Sensitivity ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Coronary Syndrome
Sign in / Sign up

Export Citation Format

Share Document