scholarly journals A Diagnostic Device for In-Situ Detection of Swine Viral Diseases: The SWINOSTICS Project

Sensors ◽  
2019 ◽  
Vol 19 (2) ◽  
pp. 407 ◽  
Author(s):  
Concetta Montagnese ◽  
Paolo Barattini ◽  
Alessandro Giusti ◽  
Gyula Balka ◽  
Ugo Bruno ◽  
...  
Keyword(s):  
Threat Assessment ◽  
Economic Damage ◽  
Diagnostic Device ◽  
Technical Requirements ◽  
Oral Fluids ◽  
Current State ◽  
Nasal Swabs ◽  
Funded Project ◽  
In Situ Detection

In this paper, we present the concept of a novel diagnostic device for on-site analyses, based on the use of advanced bio-sensing and photonics technologies to tackle emerging and endemic viruses causing swine epidemics and significant economic damage in farms. The device is currently under development in the framework of the EU Commission co-funded project. The overall concept behind the project is to develop a method for an early and fast on field detection of selected swine viruses by non-specialized personnel. The technology is able to detect pathogens in different types of biological samples, such as oral fluids, faeces, blood or nasal swabs. The device will allow for an immediate on-site threat assessment. In this work, we present the overall concept of the device, its architecture with the technical requirements, and all the used innovative technologies that contribute to the advancements of the current state of the art.

scholarly journals Cloning and Characterization of the Antigenic Membrane Protein (Amp) Gene and In Situ Detection of Amp from Malformed Flowers Infected with Japanese Hydrangea Phyllody Phytoplasma

2008 ◽  
Vol 98 (7) ◽  
pp. 769-775 ◽  
Author(s):  
R. Arashida ◽  
S. Kakizawa ◽  
Y. Ishii ◽  
A. Hoshi ◽  
H.-Y. Jung ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Membrane Protein ◽  
Detectable Level ◽  
Economic Damage ◽  
Disease Symptoms ◽  
Escherichia Coli Cells ◽  
In Situ Detection ◽  
High Diversity

A Japanese hydrangea phyllody (JHP) disease found throughout Japan causes economic damage to the horticultural industry. JHP phytoplasma-infected Japanese hydrangea plants show several disease symptoms involved in floral malformations, such as virescence, phyllody and proliferation. Here, we cloned and characterized the antigenic membrane protein (Amp) gene homolog from the JHP phytoplasma (JHP-amp), expressed the JHP-Amp protein in Escherichia coli cells, and then obtained an antibody against JHP-Amp. The antibody against JHP-Amp had no cross-reactions with the antibody against the Amp protein from a closely related onion yellows phytoplasma. This serologic specificity is probably due to the high diversity of the hydrophilic domains in the Amp proteins. The in situ detection of the JHP-Amp protein revealed that the JHP phytoplasma was localized to the phloem tissues in the malformed flower. This study shows that the JHP-Amp protein is indeed a membrane protein, which is expressed at detectable level in the JHP phytoplasma-infected hydrangea.

STORM ICE OIL WIND WAVE WATCH SYSTEM (SIOWS): WEB GIS APPLICATION FOR MONITORING THE ARCTIC

2017 ◽  
Author(s):  
Alexander Myasoedov ◽  
Alexander Myasoedov ◽  
Sergey Azarov ◽  
Sergey Azarov ◽  
Ekaterina Balashova ◽  
...  
Keyword(s):  
Satellite Data ◽  
Wind Wave ◽  
Arctic Region ◽  
Web Gis ◽  
The Arctic ◽  
Flexible Tool ◽  
In Situ Data ◽  
Current State ◽  
Watch System

Working with satellite data, has long been an issue for users which has often prevented from a wider use of these data because of Volume, Access, Format and Data Combination. The purpose of the Storm Ice Oil Wind Wave Watch System (SIOWS) developed at Satellite Oceanography Laboratory (SOLab) is to solve the main issues encountered with satellite data and to provide users with a fast and flexible tool to select and extract data within massive archives that match exactly its needs or interest improving the efficiency of the monitoring system of geophysical conditions in the Arctic. SIOWS - is a Web GIS, designed to display various satellite, model and in situ data, it uses developed at SOLab storing, processing and visualization technologies for operational and archived data. It allows synergistic analysis of both historical data and monitoring of the current state and dynamics of the "ocean-atmosphere-cryosphere" system in the Arctic region, as well as Arctic system forecasting based on thermodynamic models with satellite data assimilation.

Rapid quantification and in situ detection of nitrifying bacteria in biofilms by monoclonal antibody method

2000 ◽  
Vol 41 (4-5) ◽  
pp. 301-308 ◽  
Author(s):  
N. Noda ◽  
H. Ikuta ◽  
Y. Ebie ◽  
A. Hirata ◽  
S. Tsuneda ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Monoclonal Antibodies ◽  
Fluorescent Antibody ◽  
Nitrifying Bacteria ◽  
Fluorescent Antibody Technique ◽  
Antibody Technique ◽  
Antibody Method ◽  
In Situ Detection ◽  
Rapid Quantification

Fluorescent antibody technique by the monoclonal antibody method is very useful and helpful for the rapid quantification and in situ detection of the specific bacteria like nitrifiers in a mixed baxterial habitat such as a biofilm. In this study, twelve monoclonal antibodies against Nitrosomonas europaea (IFO14298) and sixteen against Nitrobacter winogradskyi (IFO14297) were raised from splenocytes of mice (BALB/c). It was found that these antibodies exhibited little cross reactivity against various kinds of heterotrophic bacteria. The direct cell count method using monoclonal antibodies could exactly detect and rapidly quantify N. europaea and N. winogradskyi. Moreover, the distribution of N. europaea and N. winogradskyi in a biofilm could be examined by in situ fluorescent antibody technique. It was shown that most of N. winogradskyi existed near the surface part and most of N. europaea existed at the inner part of the polyethylene glycol (PEG) gel pellet, which had entrapped activated sludge and used in a landfill leachate treatment reactor. It was suggested that this monoclonal antibody method was utilized for estimating and controlling the population of nitrifying bacteria as a quick and favorable tool.

scholarly journals Single Step In Situ Detection of Surface Protein and MicroRNA in Clustered Extracellular Vesicles Using Flow Cytometry

2021 ◽  
Vol 10 (2) ◽  
pp. 319
Author(s):  
Hee Cheol Yang ◽  
Won Jong Rhee
Keyword(s):  
Extracellular Vesicles ◽  
Liquid Biopsy ◽  
Molecular Beacon ◽  
Disease Diagnosis ◽  
Single Step ◽  
Flow Cytometer ◽  
Surface Proteins ◽  
Biomarker Detection ◽  
In Situ Detection

Because cancers are heterogeneous, it is evident that multiplexed detection is required to achieve disease diagnosis with high accuracy and specificity. Extracellular vesicles (EVs) have been a subject of great interest as sources of novel biomarkers for cancer liquid biopsy. However, EVs are nano-sized particles that are difficult to handle; thus, it is necessary to develop a method that enables efficient and straightforward EV biomarker detection. In the present study, we developed a method for single step in situ detection of EV surface proteins and inner miRNAs simultaneously using a flow cytometer. CD63 antibody and molecular beacon-21 were investigated for multiplexed biomarker detection in normal and cancer EVs. A phospholipid-polymer-phospholipid conjugate was introduced to induce clustering of the EVs analyzed using nanoparticle tracking analysis, which enhanced the detection signals. As a result, the method could detect and distinguish cancer cell-derived EVs using a flow cytometer. Thus, single step in situ detection of multiple EV biomarkers using a flow cytometer can be applied as a simple, labor- and time-saving, non-invasive liquid biopsy for the diagnosis of various diseases, including cancer.

Real-time in situ detection and source tracing of different soot

Optik ◽  
2021 ◽  
pp. 167711
Author(s):  
Enlai Wan ◽  
Zhongmou Sun ◽  
Yuzhu Liu
Keyword(s):  
Real Time ◽  
Source Tracing ◽  
In Situ Detection

scholarly journals Development of an Easy-to-Operate Underwater Raman System for Deep-Sea Cold Seep and Hydrothermal Vent In Situ Detection

Sensors ◽  
2021 ◽  
Vol 21 (15) ◽  
pp. 5090
Author(s):  
Qingsheng Liu ◽  
Jinjia Guo ◽  
Wangquan Ye ◽  
Kai Cheng ◽  
Fujun Qi ◽  
...  
Keyword(s):  
Deep Sea ◽  
Hydrothermal Vent ◽  
Methane Hydrate ◽  
Sulfur Cycle ◽  
Cold Seep ◽  
Remotely Operated Vehicle ◽  
Bacterial Mats ◽  
Optical Module ◽  
In Situ Detection

As a powerful in situ detection technique, Raman spectroscopy is becoming a popular underwater investigation method, especially in deep-sea research. In this paper, an easy-to-operate underwater Raman system with a compact design and competitive sensitivity is introduced. All the components, including the optical module and the electronic module, were packaged in an L362 × Φ172 mm titanium capsule with a weight of 20 kg in the air (about 12 kg in water). By optimising the laser coupling mode and focusing lens parameters, a competitive sensitivity was achieved with the detection limit of SO42− being 0.7 mmol/L. The first sea trial was carried out with the aid of a 3000 m grade remotely operated vehicle (ROV) “FCV3000” in October 2018. Over 20,000 spectra were captured from the targets interested, including methane hydrate, clamshell in the area of cold seep, and bacterial mats around a hydrothermal vent, with a maximum depth of 1038 m. A Raman peak at 2592 cm−1 was found in the methane hydrate spectra, which revealed the presence of hydrogen sulfide in the seeping gas. In addition, we also found sulfur in the bacterial mats, confirming the involvement of micro-organisms in the sulfur cycle in the hydrothermal field. It is expected that the system can be developed as a universal deep-sea survey and detection equipment in the near future.

scholarly journals Toward Chalcogenide Platform Infrared Sensor Dedicated to the In Situ Detection of Aromatic Hydrocarbons in Natural Waters via an Attenuated Total Reflection Spectroscopy Study

Sensors ◽  
2021 ◽  
Vol 21 (7) ◽  
pp. 2449
Author(s):  
Marion Baillieul ◽  
Emeline Baudet ◽  
Karine Michel ◽  
Jonathan Moreau ◽  
Petr Němec ◽  
...  
Keyword(s):  
Natural Water ◽  
Natural Waters ◽  
Total Reflection ◽  
Attenuated Total Reflection ◽  
Infrared Sensor ◽  
Reflection Spectroscopy ◽  
Infrared Spectral ◽  
Micro Sensor ◽  
In Situ Detection

The objective of this study is to demonstrate the successful functionalization of the surface of a chalcogenide infrared waveguide with the ultimate goal of developing an infrared micro-sensor device. First, a polyisobutylene coating was selected by testing its physico-chemical compatibility with a Ge-Sb-Se selenide surface. To simulate the chalcogenide platform infrared sensor, the detection of benzene, toluene, and ortho-, meta- and para-xylenes was efficaciously performed using a polyisobutylene layer spin-coated on 1 and 2.5 µm co-sputtered selenide films of Ge28Sb12Se60 composition deposited on a zinc selenide prism used for attenuated total reflection spectroscopy. The thickness of the polymer coating was optimized by attenuated total reflection spectroscopy to achieve the highest possible attenuation of water absorption while maintaining the diffusion rate of the pollutant through the polymer film compatible with the targeted in situ analysis. Then, natural water, i.e., groundwater, wastewater, and seawater, was sampled for detection measurement by means of attenuated total reflection spectroscopy. This study is a valuable contribution concerning the functionalization by a hydrophobic polymer compatible with a chalcogenide optical sensor designed to operate in the mid-infrared spectral range to detect in situ organic molecules in natural water.

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