scholarly journals Surface Plasmon Enhanced Light Scattering Biosensing: Size Dependence on the Gold Nanoparticle Tag

Sensors ◽  
2019 ◽  
Vol 19 (2) ◽  
pp. 323 ◽  
Author(s):  
Chih-Tsung Yang ◽  
Yi Xu ◽  
Mohammad Pourhassan-Moghaddam ◽  
Duy Tran ◽  
Lin Wu ◽  
...  
Keyword(s):  
Light Scattering ◽  
Gold Nanoparticle ◽  
Surface Plasmon ◽  
Physicochemical Characterization ◽  
Signal Enhancement ◽  
High Signal ◽  
Dot Blot ◽  
Igg Antibody ◽  
Scattered Power ◽  
Dot Blot Assay

Surface plasmon enhanced light scattering (SP-LS) is a powerful new sensing SPR modality that yields excellent sensitivity in sandwich immunoassay using spherical gold nanoparticle (AuNP) tags. Towards further improving the performance of SP-LS, we systematically investigated the AuNP size effect. Simulation results indicated an AuNP size-dependent scattered power, and predicted the optimized AuNPs sizes (i.e., 100 and 130 nm) that afford extremely high signal enhancement in SP-LS. The maximum scattered power from a 130 nm AuNP is about 1700-fold higher than that obtained from a 17 nm AuNP. Experimentally, a bio-conjugation protocol was developed by coating the AuNPs with mixture of low and high molecular weight PEG molecules. Optimal IgG antibody bioconjugation conditions were identified using physicochemical characterization and a model dot-blot assay. Aggregation prevented the use of the larger AuNPs in SP-LS experiments. As predicted by simulation, AuNPs with diameters of 50 and 64 nm yielded significantly higher SP-LS signal enhancement in comparison to the smaller particles. Finally, we demonstrated the feasibility of a two-step SP-LS protocol based on a gold enhancement step, aimed at enlarging 36 nm AuNPs tags. This study provides a blue-print for the further development of SP-LS biosensing and its translation in the bioanalytical field.

scholarly journals Correction: Investigation of plasmonic signal enhancement based on long range surface plasmon resonance with gold nanoparticle tags

2016 ◽  
Vol 4 (44) ◽  
pp. 10562-10562
Author(s):  
Chih-Tsung Yang ◽  
Lin Wu ◽  
Ping Bai ◽  
Benjamin Thierry
Keyword(s):  
Surface Plasmon Resonance ◽  
Gold Nanoparticle ◽  
Long Range ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Signal Enhancement

Correction for ‘Investigation of plasmonic signal enhancement based on long range surface plasmon resonance with gold nanoparticle tags’ by Chih-Tsung Yang et al., J. Mater. Chem. C, 2016, 4, 9897–9904.

Signal Enhancement of Surface Plasmon Resonance Imaging for Detection of Acidovorax avenae subsp. citrulli

2015 ◽  
Vol 1131 ◽  
pp. 88-94
Author(s):  
Chokchai Puttharugsa ◽  
Oraprapai Gajanandana ◽  
Orawan Himananto ◽  
Ratthasart Amarit ◽  
Armote Somboonkaew ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Gold Nanoparticle ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Limit Of Detection ◽  
Signal Enhancement ◽  
Surface Plasmon Resonance Imaging ◽  
Resonance Imaging ◽  
Self Assembled Monolayer ◽  
Spr Imaging

Signal enhancement based surface plasmon resonance imaging (SPR imaging) was developed using gold nanoparticle (AuNP) for detection of Acidovorax avenae subsp. citrulli (Aac). The antibodies against Aac, monoclonal antibody 11E5 (MAb 11E5) and polyclonal antibody MPC (PAb MPC), were covalently immobilized on the 1:40 of mixed self-assembled monolayer (mixed SAMs) surface for detection of Aac. The 107 cfu/ml of Aac was injected over the surface and was captured by immobilized antibodies on the sensing surface. PAb MPC conjugated to 10 nm of gold nanoparticle (PAb-AuNP) was flowed over the surface to enhance the SPR signal for detection of Aac. The MAb/Aac/PAb-AuNP assay provides a higher in signal enhancement than that of PAb/Aac/PAb-AuNP assay. Moreover, SPR signal using PAb-AuNP enhancement increases 23 – fold in signal enhancement when comparing to PAb enhancement. Thus, the detection of Aac based SPR imaging can be used PAb-AuNP in signal enhancement for further improvement in limit of detection (LOD).

scholarly journals AuNP Coupled Rapid Flow-Through Dot-Blot Immuno-Assay for COVID-19 (SARS-CoV-2) Explicit IgG Antibody Revelation

2021 ◽  
Author(s):  
Bijon Kumar Sil ◽  
Mohd. Raeed Jamiruddin ◽  
Md. Ahsanul Haq ◽  
Mohib Ullah Khondoker ◽  
Nowshin Jahan ◽  
...  
Keyword(s):  
Detection Sensitivity ◽  
Dot Blot ◽  
Igg Antibody ◽  
Healthy Donors ◽  
Rapid Flow ◽  
Kappa Value ◽  
Dot Blot Assay ◽  
Flow Through ◽  
Specific Igg ◽  
Immuno Assay

Background: Flow-through dot-blot assay (FT-DBA) for SARS-CoV-2 specific IgG detection will provide a reliable and affordable immunoassay for the rapid serosurveillance against COVID-19. Method: SARS-CoV-2 antigens were immobilized on nitrocellulose membrane to capture IgG immunoglobulins, which were then detected with AuNP anti-human IgG. A total of 181 samples were characterized with in-house and commercial immunoassay. The positive panel consisted of RT-PCR positive samples from patients with both <14 days and >14 days from the onset of symptoms, while the negative panel contained samples collected either from the pre-pandemic era dengue patients from healthy donors during the pandemic period. Results: In-house ELISA selected a total of 79 true seropositive and 100 seronegative samples. The sensitivity of samples with <14 days using FT-DBA was 94.7% which increased to 100% for samples >14 days. The overall detection sensitivity and specificity were 98.8% and 98%, respectively, whereas the overall PPV and NPV were 97.6% and 99%. Moreover, comparative analysis between ELISA and FT-DBA revealed clinical agreement of Cohen’s Kappa value of 0.944. Conclusion: The assay can confirm past SARS-CoV-2 infection with high accuracy within 2 minutes compared to ELISA. It can help track SARS-CoV-2 disease progression, population screening, and vaccination response.

Investigation of plasmonic signal enhancement based on long range surface plasmon resonance with gold nanoparticle tags

2016 ◽  
Vol 4 (41) ◽  
pp. 9897-9904 ◽  
Author(s):  
Chih-Tsung Yang ◽  
Lin Wu ◽  
Ping Bai ◽  
Benjamin Thierry
Keyword(s):  
Surface Plasmon Resonance ◽  
Gold Nanoparticle ◽  
Long Range ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Signal Enhancement

Gold nanoparticle (AuNP) molecular tags yield a significant signal enhancement in long range SPR-based biosensing.

APPLICATION OF REAL-TIME PRC TECHNIQUE AND REVERSE DOT-BLOT FOR DETECTION THE HIGH RISK TYPES OF HUMAN PAPILLOMAVIRUS CAUSING CERVICAL CANCER

2017 ◽  
pp. 99-103
Author(s):  
Van Bao Thang Phan ◽  
Hoang Bach Nguyen ◽  
Van Thanh Nguyen ◽  
Thi Nhu Hoa Tran ◽  
Viet Quynh Tram Ngo
Keyword(s):  
Cervical Cancer ◽  
High Risk ◽  
Real Time ◽  
Real Time Pcr ◽  
Dot Blot ◽  
The Real ◽  
Dot Blot Assay ◽  
Hpv Types ◽  
High Risk Hpv ◽  
Reverse Dot Blot

Introduction: Infection with HPV is the main cause of cervical cancer. Determining HPV infection and the types of HPV plays an important role in diagnosis, treatment and prognosis of cervicitis/cervical cancer. Aims: Determining proportion of high-risk HPV types and the occurrence of coinfection with multiple HPV types. Methods: 177 women with cervicitis or abnormal Pap smear result were enrolled in the study. Performing the real-time PCR for detecting HPV and the reverse DOT-BLOT assay for determining type of HPV in cases of positive PCR. Results: 7 types of high-risk HPV was dectected, the majority of these types were HPV type 18 (74.6%) and HPV type 16 (37.6%); the proportion of infection with only one type of HPV was 30.4% and coinfection with multiple HPV types was higher (69.6%), the coinfected cases with 2 and 3 types were dominated (32.2% and 20.3%, respectively) and the coinfected cases with 4 and 5 types were rare. Conclusion: Use of the real-time PCR and reverse DOT-BLOT assay can determine the high-risk HPV types and the occurrence of coinfection with multiple HPV types. Key words: HPV type, Reverse DOT-BLOT, real-time PCR,PCR, cervical cancer

Use of monoclonal and polyclonal antibodies to detect prostatic acid phosphatase by immunoblotting.

1986 ◽  
Vol 32 (10) ◽  
pp. 1832-1835 ◽  
Author(s):  
P C Patel ◽  
L Aubin ◽  
J Côte
Keyword(s):  
Monoclonal Antibodies ◽  
Acid Phosphatase ◽  
Western Blot ◽  
Polyclonal Antibodies ◽  
Prostatic Acid Phosphatase ◽  
The Other ◽  
Dot Blot ◽  
Western Blots ◽  
Dot Blot Assay ◽  
Polyclonal Antisera

Abstract We investigated two techniques of immunoblotting--the Western blot and the dot blot--for use in detecting prostatic acid phosphatase (PAP, EC 3.1.3.2). We used polyclonal antisera to human PAP, produced in rabbits by hyperimmunization with purified PAP, and PAP-specific monoclonal antibodies in the immunoenzymatic protocols. We conclude that PAP can be readily detected by Western blots with use of polyclonal antisera, but not with monoclonal antibodies. On the other hand, using a dot blot assay, we could easily detect PAP with both polyclonal and monoclonal antibodies.

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