scholarly journals Enlargement of Gold Nanoparticles for Sensitive Immunochromatographic Diagnostics of Potato Brown Rot

Sensors ◽  
2019 ◽  
Vol 19 (1) ◽  
pp. 153 ◽  
Author(s):  
Shyatesa C. Razo ◽  
Natalia A. Panferova ◽  
Vasily G. Panferov ◽  
Irina V. Safenkova ◽  
Natalia V. Drenova ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Detection Limit ◽  
Brown Rot ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Test Strips ◽  
Flow Test ◽  
Test Zone ◽  
Lateral Flow Test ◽  
Potato Brown Rot

Lateral flow immunoassay (LFIA) is a convenient tool for rapid field-based control of various bacterial targets. However, for many applications, the detection limits obtained by LFIA are not sufficient. In this paper, we propose enlarging gold nanoparticles’ (GNPs) size to develop a sensitive lateral flow immunoassay to detect Ralstonia solanacearum. This bacterium is a quarantine organism that causes potato brown rot. We fabricated lateral flow test strips using gold nanoparticles (17.4 ± 1.0 nm) as a label and their conjugates with antibodies specific to R. solanacearum. We proposed a signal enhancement in the test strips’ test zone due to the tetrachloroauric (III) anion reduction on the GNP surface, and the increase in size of the gold nanoparticles on the test strips was approximately up to 100 nm, as confirmed by scanning electron microscopy. Overall, the gold enhancement approach decreased the detection limit of R. solanacearum by 33 times, to as low as 3 × 104 cells∙mL–1 in the potato tuber extract. The achieved detection limit allows the diagnosis of latent infection in potato tubers. The developed approach based on gold enhancement does not complicate analyses and requires only 3 min. The developed assay together with the sample preparation and gold enlargement requires 15 min. Thus, the developed approach is promising for the development of lateral flow test strips and their subsequent introduction into diagnostic practice.

scholarly journals Modification of a nitrocellulose membrane with nanofibers for sensitivity enhancement in lateral flow test strips

RSC Advances ◽  
2021 ◽  
Vol 11 (43) ◽  
pp. 26493-26501
Author(s):  
Xue Wang ◽  
Chao-Hua Xue ◽  
Dong Yang ◽  
Shun-Tian Jia ◽  
Ya-Ru Ding ◽  
...  
Keyword(s):  
Detection Limit ◽  
Sensitivity Enhancement ◽  
Lateral Flow ◽  
Fibrous Membrane ◽  
Nitrocellulose Membrane ◽  
Test Strips ◽  
Flow Test ◽  
New Type ◽  
Lateral Flow Test

We constructed a new type of ICT strip by replacing the conventional nitrocellulose membrane with an electrospin-coated nitrocellulose (ENC) fibrous membrane, and the ICT strip could obtain an HCG detection limit of 0.22 mIU mL−1, and an LH detection limit of 0.36 mIU mL−1.

DNA detection on lateral flow test strips: enhanced signal sensitivity using LNA-conjugated gold nanoparticles

2012 ◽  
Vol 48 (62) ◽  
pp. 7714 ◽  
Author(s):  
Shiva K. Rastogi ◽  
CharLene M. Gibson ◽  
Josh R. Branen ◽  
D. Eric Aston ◽  
A. Larry Branen ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Dna Detection ◽  
Lateral Flow ◽  
Test Strips ◽  
Flow Test ◽  
Signal Sensitivity ◽  
Lateral Flow Test

scholarly journals Quantum Dot-Based Lateral Flow Test Strips for Highly Sensitive Detection of the Tetanus Antibody

ACS Omega ◽  
2019 ◽  
Vol 4 (4) ◽  
pp. 6789-6795 ◽  
Author(s):  
Junyan Wang ◽  
Hong-Min Meng ◽  
Juan Chen ◽  
Juanzu Liu ◽  
Lin Zhang ◽  
...  
Keyword(s):  
Quantum Dot ◽  
Lateral Flow ◽  
Sensitive Detection ◽  
Test Strips ◽  
Flow Test ◽  
Highly Sensitive ◽  
Lateral Flow Test ◽  
Highly Sensitive Detection

scholarly journals Analysis of free chlorine in aqueous solution at very low concentration with lateral flow tests

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
K. Uta Schwenke ◽  
Dieter Spiehl ◽  
Marcel Krauße ◽  
Laura Riedler ◽  
Anna Ruppenthal ◽  
...  
Keyword(s):  
Reaction Zone ◽  
Sample Volume ◽  
Lateral Flow ◽  
Free Chlorine ◽  
Test Strips ◽  
Flow Test ◽  
Lateral Flow Test ◽  
Lateral Flow Tests ◽  
User Friendly ◽  
Dip Test

AbstractTest strips are convenient tools for rapid, semi-quantitative analysis of a variety of parameters by dipping them for a few seconds in a sample solution followed by a simple colorimetric read-out. Their sensitivity is mainly determined by the reactivity of the test dyes on the reaction zone and is not sufficient for some applications. The detection limit of commercially available free chlorine test strips, for example, is at present not low enough to confirm the absence of this analyte as disinfectant in rinsing solutions after disinfection or to control required residual amounts of chlorine in drinking water. Therefore, we developed a user-friendly lateral flow test which is capable to detect very low amounts of free chlorine. The latter relies on a larger sample volume passing the reaction zone as compared to simple dip test strips. An amount of as low as 0.05 ppm chlorine can, however, only be detected if oxidation stable flow test substrates are used. The eventually developed flow test reaches a 10x higher sensitivity than a commercial dip test. The result is obtained within 4–5 min flow time, whereby no action is required by the user during this analysis time.

scholarly journals Magnetized Carbon Nanotube Based Lateral Flow Immunoassay for Visual Detection of Complement Factor B

Molecules ◽  
2019 ◽  
Vol 24 (15) ◽  
pp. 2759 ◽  
Author(s):  
Yan Huang ◽  
Tingting Wu ◽  
Fang Wang ◽  
Kun Li ◽  
Lisheng Qian ◽  
...  
Keyword(s):  
Carbon Nanotube ◽  
Human Blood ◽  
Visual Detection ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Complement Factor ◽  
Factor B ◽  
Complement Factor B ◽  
Flow Test ◽  
Lateral Flow Test

The authors describe a magnetized carbon nanotube (MCNT) based lateral flow immunoassay (LFI) for visual detection of complement factor B (CFB) in blood. MCNT was prepared by decorating magnetic Fe3O4 nanoparticles on multi-walled CNT surface and used as a colored tag for LFI. Monoclonal antibody (mAb, Ab1) of CFB was covalently immobilized on the MCNT surface via diimide-activated conjugation between the carboxyl groups on the MCNT surface and amino groups of antibodies. Polyclonal antibody of CFB (Ab2) and the secondary antibody were used to prepare the lateral flow test strips. The assay involved: (1) the capture of CFB in blood with the mAb-functionalized MCNT; (2) magnetic separation of the formed CFB-mAb-MCNT and excess of mAb-MCNT from the blood with an external magnet; (3) lateral flow test to capture the CFB-mAb-MCNT complex on the test zone and the excess of mAb-MCNT on the control zone; (4) Recording the intensities of the produced the characteristic brown bands with a portable strip reader and quantitating the concentration of CFB. The proof-of-concept was demonstrated by testing CFB in the buffer, and the detection limit was 5 ng mL−1 under the optimized analytical parameters. CFB in 1 μL of human blood was detected successfully in 30 min with this LFI and the results had a high correlation with commercial ELISA kit. Thence, the MCNT-based LFI offers a rapid and low-cost tool for detecting CFB in human blood directly.

Multiplex Lateral-Flow Test Strips Fabricated by Two-Dimensional Shaping

2008 ◽  
Vol 1 (1) ◽  
pp. 124-129 ◽  
Author(s):  
Erin M. Fenton ◽  
Monica R. Mascarenas ◽  
Gabriel P. López ◽  
Scott S. Sibbett
Keyword(s):  
Lateral Flow ◽  
Two Dimensional ◽  
Test Strips ◽  
Flow Test ◽  
Lateral Flow Test
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