scholarly journals Label-Free Impedance Sensing of Aflatoxin B1 with Polyaniline Nanofibers/Au Nanoparticle Electrode Array

Sensors ◽  
2018 ◽  
Vol 18 (5) ◽  
pp. 1320 ◽  
Author(s):  
Ajay Kumar Yagati ◽  
Sachin Ganpat Chavan ◽  
Changyoon Baek ◽  
Min-Ho Lee ◽  
Junhong Min
Keyword(s):  
Aflatoxin B1 ◽  
Electrode Array ◽  
Au Nanoparticle ◽  
Label Free ◽  
Impedance Sensing ◽  
Polyaniline Nanofibers

A label-free aptasensor based on a dual-emission fluorescent strategy for aflatoxin B1 detection

2021 ◽  
pp. 130561
Author(s):  
Yao-Yao Fan ◽  
Jun Li ◽  
Li Fan ◽  
Jie Wen ◽  
Jing Zhang ◽  
...  
Keyword(s):  
Aflatoxin B1 ◽  
Label Free ◽  
Dual Emission

Measurements of Nonlinear Electrical Impedances by Virtue of Induced Conformational Changes in DNAs

2010 ◽  
Vol 22 (5) ◽  
pp. 601-607 ◽  
Author(s):  
Takatoki Yamamoto ◽  
◽  
Sangwook Lee ◽  
Teruo Fujii ◽  
Keyword(s):  
Aqueous Solution ◽  
Conformational Changes ◽  
Electrical Impedance ◽  
Dna Analysis ◽  
Electrical Impedance Spectroscopy ◽  
Label Free ◽  
Impedance Sensing ◽  
Fully Integrated ◽  
Impedance Response ◽  
Strength Dependence

A method for label-free electrical impedance sensing of DNA is proposed, and experimentally demonstrated using a micro Electrical Impedance Spectroscopy (µ- EIS) device. The method features not only the detection of DNA without any labelling, but also the control of the conformation that would enhance the electrical impedance signal. In order to conduct semiautomated measurements controlled by an external PC, a microfluidic chip made of a silicone elastomer of polydimethylsiloxane (PDMS), a measurement chip embedded with micro-electrodes, and a micropump chip are fully integrated in the µ-EIS device. The µ-EIS device is capable of detecting DNA concentrations of a few nM in aqueous solution of a few pL in volume by virtue of the conformation-enhanced nonlinear impedance response. As a first demonstration of conformational-change-induced DNA analysis, the frequency and the electric field strength dependence of various lengths of DNA are evaluated.

Electric cell-substrate impedance sensing in kidney research

2019 ◽  
Author(s):  
Takamasa Iwakura ◽  
Julian A Marschner ◽  
Zhi Bo Zhao ◽  
Monika Katarzyna Świderska ◽  
Hans-Joachim Anders
Keyword(s):  
Continuous Monitoring ◽  
Adherent Cells ◽  
Label Free ◽  
Impedance Sensing ◽  
Barrier Integrity ◽  
Practical Applications ◽  
Non Invasive ◽  
Glomerular Epithelial Cells ◽  
Cell Substrate ◽  
Wide Range

Abstract Electric cell-substrate impedance sensing (ECIS) is a quantitative, label-free, non-invasive analytical method allowing continuous monitoring of the behaviour of adherent cells by online recording of transcellular impedance. ECIS offers a wide range of practical applications to study cell proliferation, migration, differentiation, toxicity and monolayer barrier integrity. All of these applications are relevant for basic kidney research, e.g. on endothelial cells, tubular and glomerular epithelial cells. This review gives an overview on the fundamental principles of the ECIS technology. We name strengths and remaining hurdles for practical applications, present an ECIS array reuse protocol, and review its past, present and potential future contributions to preclinical kidney research.

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