scholarly journals Single-Step Purification of Monomeric l-Selectin via Aptamer Affinity Chromatography

Sensors ◽  
2017 ◽  
Vol 17 (2) ◽  
pp. 226 ◽  
Author(s):  
Christian Kuehne ◽  
Stefanie Wedepohl ◽  
Jens Dernedde
Keyword(s):  
Affinity Chromatography ◽  
Single Step ◽  
Single Step Purification

Single-step purification of urease by affinity chromatography

1974 ◽  
Vol 20 (4) ◽  
pp. 623-630 ◽  
Author(s):  
Bassanio L. Wong ◽  
Charles R. Shobe
Keyword(s):  
Enzyme Activity ◽  
Affinity Chromatography ◽  
Single Step ◽  
Jack Bean Urease ◽  
Jack Bean ◽  
Specific Activities ◽  
Single Step Purification

Single-step purification of urease (urea aminohydrolase; EC. 3.5.1.5) from cell-free extracts of Proteus morganii and from partially purified preparations of jack bean urease were achieved in less than 90 min by affinity chromatography on hydroxyurea-substituted beaded agarose columns. The specific activities of the purified enzymes were as high as, or higher than, those reported by other authors for urease preparations obtained by conventional techniques. In addition, yields of enzyme activity were routinely 6 to 100 times greater than recoveries previously reported for this enzyme.

Single-step purification of cyclotides using affinity chromatography

Biopolymers ◽  
2017 ◽  
Vol 108 (3) ◽  
pp. e23010 ◽  
Author(s):  
Shaikh Jamal Uddin ◽  
Taj Muhammad ◽  
Md Shafiullah ◽  
Blazej Slazak ◽  
Razina Rouf ◽  
...  
Keyword(s):  
Affinity Chromatography ◽  
Single Step ◽  
Single Step Purification
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