Expression and Single-Step Purification of Enzymatically Active Vaccinia Virus Thymidine Kinase Containing an Engineered Oligohistidine Domain by Immobilized Metal Affinity-Chromatography

C.A. Franke; D.E. Hruby

doi:10.1006/prep.1993.1015

Expression and Single-Step Purification of Enzymatically Active Vaccinia Virus Thymidine Kinase Containing an Engineered Oligohistidine Domain by Immobilized Metal Affinity-Chromatography

Protein Expression and Purification ◽

10.1006/prep.1993.1015 ◽

1993 ◽

Vol 4 (2) ◽

pp. 101-109 ◽

Cited By ~ 11

Author(s):

C.A. Franke ◽

D.E. Hruby

Keyword(s):

Affinity Chromatography ◽

Vaccinia Virus ◽

Thymidine Kinase ◽

Single Step ◽

Immobilized Metal Affinity Chromatography ◽

Metal Affinity ◽

Single Step Purification

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Single-step purification of recombinant green fluorescent protein on expanded beds of immobilized metal affinity chromatography media

Biochemical Engineering Journal ◽

10.1016/j.bej.2008.07.010 ◽

2008 ◽

Vol 42 (3) ◽

pp. 301-307 ◽

Cited By ~ 15

Author(s):

Sohel Dalal ◽

Smita Raghava ◽

M.N. Gupta

Keyword(s):

Green Fluorescent Protein ◽

Affinity Chromatography ◽

Fluorescent Protein ◽

Single Step ◽

Immobilized Metal Affinity Chromatography ◽

Metal Affinity ◽

Single Step Purification ◽

Green Fluorescent

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Single-Step Purification of Native Miraculin Using Immobilized Metal-Affinity Chromatography

Journal of Agricultural and Food Chemistry ◽

10.1021/jf9004065 ◽

2009 ◽

Vol 57 (12) ◽

pp. 5148-5151 ◽

Cited By ~ 14

Author(s):

Narendra Duhita ◽

Kyoko Hiwasa-Tanase ◽

Shigeki Yoshida ◽

Hiroshi Ezura

Keyword(s):

Affinity Chromatography ◽

Single Step ◽

Immobilized Metal Affinity Chromatography ◽

Metal Affinity ◽

Single Step Purification

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Single step purification of biologically active recombinant rat basic fibroblast growth factor by immobilized metal affinity chromatography

Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology ◽

10.1016/0167-4838(95)00060-8 ◽

1995 ◽

Vol 1250 (1) ◽

pp. 29-34 ◽

Cited By ~ 11

Author(s):

Michael Kroiher ◽

Simona Raffioni ◽

Robert E. Steele

Keyword(s):

Growth Factor ◽

Affinity Chromatography ◽

Fibroblast Growth Factor ◽

Basic Fibroblast Growth Factor ◽

Single Step ◽

Biologically Active ◽

Immobilized Metal Affinity Chromatography ◽

Fibroblast Growth ◽

Metal Affinity ◽

Single Step Purification

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Single-step purification of a bacterially expressed antibody Fv fragment by immobilized metal affinity chromatography in the presence of betaine

Journal of Chromatography A ◽

10.1016/0021-9673(93)83034-p ◽

1993 ◽

Vol 657 (1) ◽

pp. 55-61 ◽

Cited By ~ 23

Author(s):

Lars-Oliver Essen ◽

Arne Skerra

Keyword(s):

Affinity Chromatography ◽

Single Step ◽

Immobilized Metal Affinity Chromatography ◽

Metal Affinity ◽

Single Step Purification

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Faculty Opinions recommendation of Evaluating immobilized metal affinity chromatography for the selection of histidine-containing peptides in comparative proteomics.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1014119.192808 ◽

2003 ◽

Author(s):

George Janini

Keyword(s):

Affinity Chromatography ◽

Comparative Proteomics ◽

Immobilized Metal Affinity Chromatography ◽

Metal Affinity ◽

Selection Of

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Faculty Opinions recommendation of Immobilized metal affinity chromatography as a drug discovery platform for metalloenzyme inhibitors.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.738685288.793578992 ◽

2020 ◽

Author(s):

Seth Cohen

Keyword(s):

Drug Discovery ◽

Affinity Chromatography ◽

Immobilized Metal Affinity Chromatography ◽

Metal Affinity

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Characterization of mercury-binding proteins in human neuroblastoma SK-N-SH cells with immobilized metal affinity chromatography

Talanta ◽

10.1016/j.talanta.2017.10.028 ◽

2018 ◽

Vol 178 ◽

pp. 811-817 ◽

Cited By ~ 16

Author(s):

Yiling Li ◽

Bin He ◽

Ligang Hu ◽

Xiu Huang ◽

Zhaojun Yun ◽

...

Keyword(s):

Affinity Chromatography ◽

Binding Proteins ◽

Immobilized Metal Affinity Chromatography ◽

Human Neuroblastoma ◽

Metal Affinity

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Studies with an immobilized metal affinity chromatography cassette system involving binuclear triazacyclononane-derived ligands: Automation of batch adsorption measurements with tagged recombinant proteins

Journal of Chromatography A ◽

10.1016/j.chroma.2014.05.030 ◽

2014 ◽

Vol 1351 ◽

pp. 61-69 ◽

Cited By ~ 15

Author(s):

Martin Petzold ◽

Campbell J. Coghlan ◽

Milton T.W. Hearn

Keyword(s):

Affinity Chromatography ◽

Recombinant Proteins ◽

Immobilized Metal Affinity Chromatography ◽

Batch Adsorption ◽

Metal Affinity

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Construction of a modified penicillin-binding protein 2a from methicillin-resistant Staphylococcus aureus and purification by immobilized metal affinity chromatography.

Journal of Bacteriology ◽

10.1128/jb.176.5.1539-1541.1994 ◽

1994 ◽

Vol 176 (5) ◽

pp. 1539-1541 ◽

Cited By ~ 4

Author(s):

C Y Wu ◽

L C Blaszczak ◽

M C Smith ◽

P L Skatrud

Keyword(s):

Staphylococcus Aureus ◽

Affinity Chromatography ◽

Methicillin Resistant Staphylococcus Aureus ◽

Binding Protein ◽

Immobilized Metal Affinity Chromatography ◽

Penicillin Binding Protein ◽

Methicillin Resistant ◽

Metal Affinity

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Benchtop Immobilized Metal Affinity Chromatography, Reconstitution and Assay of a Polyhistidine Tagged Metalloenzyme for the Undergraduate Laboratory

Journal of Visualized Experiments ◽

10.3791/58012 ◽

2018 ◽

Author(s):

Keri L Colabroy ◽

Katlyn Mayer

Keyword(s):

Affinity Chromatography ◽

Immobilized Metal Affinity Chromatography ◽

Metal Affinity ◽

Undergraduate Laboratory

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