scholarly journals Detection of the Vascular Endothelial Growth Factor with a Novel Bioluminescence Resonance Energy Transfer Pair Using a Two-Component System

Sensors ◽  
2017 ◽  
Vol 17 (12) ◽  
pp. 145 ◽  
Author(s):  
Tobias Wimmer ◽  
Eva Schroeter ◽  
Birgit Lorenz ◽  
Knut Stieger
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Energy Transfer ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Vascular Endothelial ◽  
Bioluminescence Resonance Energy Transfer ◽  
Two Component System ◽  
Component System ◽  
Two Component ◽  
Endothelial Growth Factor

A highly sensitive aptasensor for vascular endothelial growth factor based on fluorescence resonance energy transfer from upconversion nanoparticles to MoS2 nanosheets

2020 ◽  
Vol 12 (36) ◽  
pp. 4466-4472
Author(s):  
Yunxia Yuan ◽  
Huichun Yu ◽  
Yong Yin
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Energy Transfer ◽  
Growth Factor ◽  
Fluorescence Resonance Energy Transfer ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Upconversion Nanoparticles ◽  
Vascular Endothelial ◽  
Fluorescence Resonance ◽  
Endothelial Growth Factor

A novel sensitive aptasensor for vascular endothelial growth factor-165 (VEGF165) was constructed based on fluorescence resonance energy transfer (FRET) between upconversion nanoparticles (UCNPs) and MoS2 nanosheets.

scholarly journals Enhanced brightness of bacterial luciferase by bioluminescence resonance energy transfer

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Tomomi Kaku ◽  
Kazunori Sugiura ◽  
Tetsuyuki Entani ◽  
Kenji Osabe ◽  
Takeharu Nagai
Keyword(s):  
Energy Transfer ◽  
Mammalian Cells ◽  
Fluorescent Protein ◽  
Color Change ◽  
Yellow Fluorescent Protein ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Bioluminescence Resonance Energy Transfer ◽  
Bacterial Luciferase ◽  
Bacterial Bioluminescence

AbstractUsing the lux operon (luxCDABE) of bacterial bioluminescence system as an autonomous luminous reporter has been demonstrated in bacteria, plant and mammalian cells. However, applications of bacterial bioluminescence-based imaging have been limited because of its low brightness. Here, we engineered the bacterial luciferase (heterodimer of luxA and luxB) by fusion with Venus, a bright variant of yellow fluorescent protein, to induce bioluminescence resonance energy transfer (BRET). By using decanal as an externally added substrate, color change and ten-times enhancement of brightness was achieved in Escherichia coli when circularly permuted Venus was fused to the C-terminus of luxB. Expression of the Venus-fused luciferase in human embryonic kidney cell lines (HEK293T) or in Nicotiana benthamiana leaves together with the substrate biosynthesis-related genes (luxC, luxD and luxE) enhanced the autonomous bioluminescence. We believe the improved luciferase will forge the way towards the potential development of autobioluminescent reporter system allowing spatiotemporal imaging in live cells.

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