A Fluorescent Nanoprobe Based on Graphene Oxide Fluorescence Resonance Energy Transfer for the Rapid Determination of Oncoprotein Vascular Endothelial Growth Factor (VEGF)

2013 ◽  
Vol 67 (11) ◽  
pp. 1270-1274 ◽  
Author(s):  
Sheng-E Wang ◽  
Shihui Si
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Graphene Oxide ◽  
Energy Transfer ◽  
Growth Factor ◽  
Rapid Determination ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Vascular Endothelial ◽  
Endothelial Growth Factor

A highly sensitive aptasensor for vascular endothelial growth factor based on fluorescence resonance energy transfer from upconversion nanoparticles to MoS2 nanosheets

2020 ◽  
Vol 12 (36) ◽  
pp. 4466-4472
Author(s):  
Yunxia Yuan ◽  
Huichun Yu ◽  
Yong Yin
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Energy Transfer ◽  
Growth Factor ◽  
Fluorescence Resonance Energy Transfer ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Upconversion Nanoparticles ◽  
Vascular Endothelial ◽  
Fluorescence Resonance ◽  
Endothelial Growth Factor

A novel sensitive aptasensor for vascular endothelial growth factor-165 (VEGF165) was constructed based on fluorescence resonance energy transfer (FRET) between upconversion nanoparticles (UCNPs) and MoS2 nanosheets.

A novel approach for the discrimination of culture medium from Vascular Endothelial Growth Factor (VEGF) overexpressing colorectal cancer cells

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Sinem Tunçer ◽  
Rafig Gurbanov
Keyword(s):  
Colorectal Cancer ◽  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
Ftir Spectroscopy ◽  
Cancer Cells ◽  
Lower Amount ◽  
Vascular Endothelial ◽  
Colorectal Cancer Cells ◽  
Endothelial Growth Factor

AbstractObjectivesThe expression level of Vascular Endothelial Growth Factor (VEGF) is assumed as a prognostic marker for several tumor types, including colorectal cancer. Therefore, the determination of pre- and post-therapy levels of VEGF appears to have great value in the assessment of tumor prognosis. Enzyme-Linked Immunosorbent Assay (ELISA) is commonly used for the determination of serum or plasma VEGF levels, but the method is costly and time-consuming. In this study, we aimed to describe a rapid and cost-effective analysis method to discriminate VEGF overexpressing colorectal cancer-derived conditioned medium (CM).MethodsAttenuated Total Reflection (ATR)-Fourier Transform Infrared (FTIR) spectroscopy, combined with Principal Component Analysis (PCA) and Linear Discriminant Analysis (LDA), was used to differentiate VEGF overexpressing colorectal cancer cell line CM from CM obtained from the corresponding control cells which express and secrete relatively lower amount of VEGF.ResultsSamples belong to VEGF overexpressing colorectal cancer cells were clearly distinguished from the control group with very high PC scores as PC1 + PC2 = 96%. Besides, a 100% accurate distinction between these two groups was achieved by the LDA analysis.ConclusionsATR-FTIR spectroscopy combined with pattern recognition techniques was able to discriminate CM of VEGF overexpressing colorectal cancer cells with high efficiency and accuracy.

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