scholarly journals Label-Free Potentiometry for Detecting DNA Hybridization Using Peptide Nucleic Acid and DNA Probes

Sensors ◽  
2013 ◽  
Vol 13 (2) ◽  
pp. 2267-2278 ◽  
Author(s):  
Tatsuro Goda ◽  
Ankit Singi ◽  
Yasuhiro Maeda ◽  
Akira Matsumoto ◽  
Masaki Torimura ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Dna Hybridization ◽  
Peptide Nucleic Acid ◽  
Dna Probes ◽  
Label Free

Label-free DNA biosensor based on a peptide nucleic acid-functionalized microstructured optical fiber-Bragg grating

2013 ◽  
Vol 18 (5) ◽  
pp. 057004 ◽  
Author(s):  
Alessandro Candiani ◽  
Alessandro Bertucci ◽  
Sara Giannetti ◽  
Maria Konstantaki ◽  
Alex Manicardi ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Optical Fiber ◽  
Fiber Bragg Grating ◽  
Peptide Nucleic Acid ◽  
Dna Biosensor ◽  
Bragg Grating ◽  
Label Free ◽  
Microstructured Optical Fiber ◽  
Free Dna ◽  
Optical Fiber Bragg Grating

scholarly journals Rapid label-free visual detection of KRAS mutations using peptide nucleic acid and unmodified gold nanoparticles

RSC Advances ◽  
2017 ◽  
Vol 7 (77) ◽  
pp. 48554-48560 ◽  
Author(s):  
Xihong Zhao ◽  
Chii-Wann Lin
Keyword(s):  
Colorectal Cancer ◽  
Gold Nanoparticles ◽  
Nucleic Acid ◽  
Peptide Nucleic Acid ◽  
Visual Detection ◽  
Label Free ◽  
Kras Mutations

Colorectal cancer (CRC) is among the most commonly diagnosed cancers worldwide.

scholarly journals Methods for assessing DNA hybridization of peptide nucleic acid–titanium dioxide nanoconjugates

2008 ◽  
Vol 383 (2) ◽  
pp. 226-235 ◽  
Author(s):  
Eric M.B. Brown ◽  
Tatjana Paunesku ◽  
AiGuo Wu ◽  
K. Ted Thurn ◽  
Benjamin Haley ◽  
...  
Keyword(s):  
Titanium Dioxide ◽  
Nucleic Acid ◽  
Dna Hybridization ◽  
Peptide Nucleic Acid

scholarly journals Label-Free Dengue Detection Utilizing PNA/DNA Hybridization Based on the Aggregation Process of Unmodified Gold Nanoparticles

2014 ◽  
Vol 2014 ◽  
pp. 1-5 ◽  
Author(s):  
Samsulida Abdul Rahman ◽  
Rafidah Saadun ◽  
Nur Ellina Azmi ◽  
Nurhayati Ariffin ◽  
Jaafar Abdullah ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Dna Hybridization ◽  
Peptide Nucleic Acid ◽  
Virus Detection ◽  
Label Free ◽  
Color Changes ◽  
Phosphate Backbone ◽  
Single Base Mismatch ◽  
Target Dna ◽  
Base Mismatch

A label-free optical detection method based on PNA/DNA hybridization using unmodified gold nanoparticles (AuNPs) for dengue virus detection has been successfully developed. In this study, no immobilization method is involved and the hybridization of PNA/DNA occurs directly in solution. Unmodified AuNPs undergo immediate aggregation in the presence of neutral charge peptide nucleic acid (PNA) due to the coating of PNA on AuNPs surface. However, in the presence of complementary targets DNA, the hybridization of PNA probe with target DNA forms negatively charged complexes due to the negatively charged phosphate backbone of the target DNA. The negatively charged complexes adsorbed onto the AuNPs surface ensure sufficient charge repulsion, need for AuNPs dispersion, and stability in solution. The detection procedure is a naked eye method based on immediate color changes and also through UV-vis adsorption spectra. The selectivity of the proposed method was studied successfully by single base mismatch and noncomplementary target DNA.

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