scholarly journals Rapid label-free visual detection of KRAS mutations using peptide nucleic acid and unmodified gold nanoparticles

RSC Advances ◽  
2017 ◽  
Vol 7 (77) ◽  
pp. 48554-48560 ◽  
Author(s):  
Xihong Zhao ◽  
Chii-Wann Lin
Keyword(s):  
Colorectal Cancer ◽  
Gold Nanoparticles ◽  
Nucleic Acid ◽  
Peptide Nucleic Acid ◽  
Visual Detection ◽  
Label Free ◽  
Kras Mutations

Colorectal cancer (CRC) is among the most commonly diagnosed cancers worldwide.

Label-free peptide nucleic acid biosensor for visual detection of multiple strains of influenza A virus suitable for field applications

2020 ◽  
Vol 1093 ◽  
pp. 123-130 ◽  
Author(s):  
Naveen Kumar ◽  
Sandeep Bhatia ◽  
Atul Kumar Pateriya ◽  
Richa Sood ◽  
S. Nagarajan ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Influenza A Virus ◽  
Peptide Nucleic Acid ◽  
Influenza A ◽  
Visual Detection ◽  
Label Free ◽  
Free Peptide ◽  
Multiple Strains

scholarly journals Detecting KRAS mutations in peripheral blood of colorectal cancer patients by peptide nucleic acid clamp PCR

2012 ◽  
Vol 4 (5) ◽  
pp. 790-794 ◽  
Author(s):  
SHOZO MIYANO ◽  
KISABURO HANAZAWA ◽  
TOSHIAKI KITABATAKE ◽  
MINORU FUJISAWA ◽  
KUNIAKI KOJIMA
Keyword(s):  
Colorectal Cancer ◽  
Nucleic Acid ◽  
Cancer Patients ◽  
Peripheral Blood ◽  
Peptide Nucleic Acid ◽  
Kras Mutations ◽  
Colorectal Cancer Patients

Rapid label-free visual assay for the detection and quantification of viral RNA using peptide nucleic acid (PNA) and gold nanoparticles (AuNPs)

2013 ◽  
Vol 795 ◽  
pp. 1-7 ◽  
Author(s):  
Vinay G. Joshi ◽  
Kantaraja Chindera ◽  
Arvind Kumar Singh ◽  
Aditya P. Sahoo ◽  
Vikas D. Dighe ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Nucleic Acid ◽  
Peptide Nucleic Acid ◽  
Viral Rna ◽  
Label Free ◽  
Visual Assay ◽  
Detection And Quantification

Synthesis of Stable Peptide Nucleic Acid-Modified Gold Nanoparticles and their Assembly onto Gold Surfaces

2013 ◽  
Vol 52 (15) ◽  
pp. 4217-4220 ◽  
Author(s):  
Philipp Anstaett ◽  
Yuanhui Zheng ◽  
Thibaut Thai ◽  
Alison M. Funston ◽  
Udo Bach ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Nucleic Acid ◽  
Peptide Nucleic Acid ◽  
Gold Surfaces

Label-free DNA biosensor based on a peptide nucleic acid-functionalized microstructured optical fiber-Bragg grating

2013 ◽  
Vol 18 (5) ◽  
pp. 057004 ◽  
Author(s):  
Alessandro Candiani ◽  
Alessandro Bertucci ◽  
Sara Giannetti ◽  
Maria Konstantaki ◽  
Alex Manicardi ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Optical Fiber ◽  
Fiber Bragg Grating ◽  
Peptide Nucleic Acid ◽  
Dna Biosensor ◽  
Bragg Grating ◽  
Label Free ◽  
Microstructured Optical Fiber ◽  
Free Dna ◽  
Optical Fiber Bragg Grating

scholarly journals Label-Free Dengue Detection Utilizing PNA/DNA Hybridization Based on the Aggregation Process of Unmodified Gold Nanoparticles

2014 ◽  
Vol 2014 ◽  
pp. 1-5 ◽  
Author(s):  
Samsulida Abdul Rahman ◽  
Rafidah Saadun ◽  
Nur Ellina Azmi ◽  
Nurhayati Ariffin ◽  
Jaafar Abdullah ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Dna Hybridization ◽  
Peptide Nucleic Acid ◽  
Virus Detection ◽  
Label Free ◽  
Color Changes ◽  
Phosphate Backbone ◽  
Single Base Mismatch ◽  
Target Dna ◽  
Base Mismatch

A label-free optical detection method based on PNA/DNA hybridization using unmodified gold nanoparticles (AuNPs) for dengue virus detection has been successfully developed. In this study, no immobilization method is involved and the hybridization of PNA/DNA occurs directly in solution. Unmodified AuNPs undergo immediate aggregation in the presence of neutral charge peptide nucleic acid (PNA) due to the coating of PNA on AuNPs surface. However, in the presence of complementary targets DNA, the hybridization of PNA probe with target DNA forms negatively charged complexes due to the negatively charged phosphate backbone of the target DNA. The negatively charged complexes adsorbed onto the AuNPs surface ensure sufficient charge repulsion, need for AuNPs dispersion, and stability in solution. The detection procedure is a naked eye method based on immediate color changes and also through UV-vis adsorption spectra. The selectivity of the proposed method was studied successfully by single base mismatch and noncomplementary target DNA.

scholarly journals Inhibitory effect of peptide nucleic acid on the growth of colorectal cancer LS-174T cells

2008 ◽  
Vol 16 (11) ◽  
pp. 1179
Author(s):  
Xu-Hai Zhao ◽  
Gui-Yu Wang ◽  
Yi-Hui Wang ◽  
Shi-Xiong Jiang ◽  
Xi-Shan Wang
Keyword(s):  
Colorectal Cancer ◽  
Nucleic Acid ◽  
Peptide Nucleic Acid ◽  
Inhibitory Effect
Sign in / Sign up

Export Citation Format

Share Document