scholarly journals Subtractive Inhibition Assay for the Detection of E. coli O157:H7 Using Surface Plasmon Resonance

Sensors ◽  
2011 ◽  
Vol 11 (3) ◽  
pp. 2728-2739 ◽  
Author(s):  
Yixian Wang ◽  
Zunzhong Ye ◽  
Chengyan Si ◽  
Yibin Ying
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Inhibition Assay ◽  
E Coli

Rapid Surface Plasmon Resonance-Based Inhibition Assay of Deoxynivalenol

2003 ◽  
Vol 51 (20) ◽  
pp. 5843-5848 ◽  
Author(s):  
Anna J. Tüdös ◽  
Elly R. Lucas-van den Bos ◽  
Edwin C. A. Stigter
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Inhibition Assay

Detection to E. Coli O157 by Surface Plasmon Resonance Immunosensor

2012 ◽  
Vol 518-523 ◽  
pp. 305-308 ◽  
Author(s):  
Hui Yao ◽  
Xiao Hui Zhang ◽  
Hong Zong Yin
Keyword(s):  
Silver Nanoparticles ◽  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
The Self ◽  
Bacteria Detection ◽  
Gold Substrate ◽  
Self Assembled Monolayer ◽  
E Coli ◽  
Coli Antibody

This work describes an approach for the development of two bacteria biosensors based on surface plasmon resonance (SPR) technique. The first biosensor was based on functionalized gold substrate and the second one on immobilized silver nanoparticles. For the first biosensor, the gold substrate was functionalized with acid–thiol using the self-assembled monolayer technique, while the second one was functionalized with silver nanoparticles immobilized on modified gold substrate. A polyclonal anti-Escherichia coli antibody was immobilized for specific (E. coli) bacteria detection. Detection limit with a good reproducibility of 104 and 103 cfu•mL−1 of E. coli bacteria has been obtained for the first biosensor and for the second one respectively.

scholarly journals Surface Plasmon Resonance Assay for Real-Time Monitoring of Somatic Coliphages in Wastewaters

2008 ◽  
Vol 74 (13) ◽  
pp. 4054-4058 ◽  
Author(s):  
Cristina García-Aljaro ◽  
Xavier Muñoz-Berbel ◽  
A. Toby A. Jenkins ◽  
Anicet R. Blanch ◽  
Francesc Xavier Muñoz
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Limit Of Detection ◽  
Mass Density ◽  
Binding Constants ◽  
Somatic Coliphages ◽  
E Coli ◽  
Low Concentrations ◽  
High Concentrations

ABSTRACT The surface plasmon resonance (SPR) technique is a well-established method for the measurement of molecules binding to surfaces and the quantification of binding constants between surface-immobilized proteins and proteins in solution. In this paper we describe an extension of the methodology to study bacteriophage-bacterium interactions. A two-channel microfluidic SPR sensor device was used to detect the presence of somatic coliphages, a group of bacteriophages that have been proposed as fecal pollution indicators in water, using their host, Escherichia coli WG5, as a target for their selective detection. The bacterium, E. coli WG5, was immobilized on gold sensor chips using avidin-biotin and bacteriophages extracted from wastewater added. The initial binding of the bacteriophage was observed at high concentrations, and a separate, time-delayed cell lysis event also was observed, which was sensitive to bacteriophage at low concentrations. As few as 1 PFU/ml of bacteriophage injected into the chamber could be detected after a phage incubation period of 120 min, which equates to an approximate limit of detection of around 102 PFU/ml. The bacteriophage-bacterium interaction appeared to cause a structural change in the surface-bound bacteria, possibly due to collapse of the cell, which was observed as an increase in mass density on the sensor chip. These results suggest that this methodology could be employed for future biosensor technologies and for quantification of the bacteriophage concentration.

Surface plasmon resonance-based inhibition assay for real-time detection of Cryptosporidium parvum oocyst

2008 ◽  
Vol 42 (6-7) ◽  
pp. 1693-1699 ◽  
Author(s):  
Chang Duk Kang ◽  
Cuong Cao ◽  
Jeewon Lee ◽  
Insung S. Choi ◽  
Byung Woo Kim ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Real Time ◽  
Surface Plasmon ◽  
Cryptosporidium Parvum ◽  
Plasmon Resonance ◽  
Inhibition Assay ◽  
Cryptosporidium Parvum Oocyst ◽  
Real Time Detection

Interaction of ribosome recycling factor and elongation factor EF-G with E. coli ribosomes studied by the surface plasmon resonance technique

2000 ◽  
Vol 5 (12) ◽  
pp. 953-963 ◽  
Author(s):  
Tetsuya Ishino ◽  
Kenji Atarashi ◽  
Susumu Uchiyama ◽  
Tohru Yamami ◽  
Yukari Saihara ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Elongation Factor ◽  
Ribosome Recycling Factor ◽  
Resonance Technique ◽  
E Coli

scholarly journals Novel surface plasmon resonance biosensor that uses full-length Det7 phage tail protein for rapid and selective detection of Salmonella enterica serovar Typhimurium

2019 ◽  
Author(s):  
Seok Hywan Hyeon ◽  
Woon Ki Lim ◽  
Hae Ja Shin
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Salmonella Enterica ◽  
Full Length ◽  
E Coli ◽  
Spr Biosensor ◽  
Phage Tail ◽  
Serovar Typhimurium ◽  
Tail Protein

AbstractWe report a novel surface plasmon resonance (SPR) biosensor that uses the full-length Det7 phage tail protein (Det7T) to rapidly and selectively detect Salmonella enterica serovar Typhimurium. Det7T, which was obtained using recombinant protein expression and purification in Escherichia coli, demonstrated a size of ∼75 kDa upon SDS-PAGE and was homotrimeric in its native structure. Micro-agglutination and TEM data revealed that the protein specifically bound to the host, S. Typhimurium, but not to non-host E. coli K-12 cells. The observed protein agglutination occurred over a concentration range of 1.5∼25 μg.ml−1. The Det7T proteins were immobilized on gold-coated surfaces using amine-coupling to generate a novel Det7T-functionalized SPR biosensor, wherein the specific binding of these proteins with bacteria was detected by SPR. We observed rapid detection of (∼ 20 min) and typical binding kinetics with S. Typhimurium in the range of 5 × 104-5 × 107 CFU.ml−1, but not with E. coli at any tested concentration, indicating that the sensor exhibited recognition specificity. Similar binding was observed with 10% apple juice spiked with S. Typhimurium, suggesting that this strategy could be expanded for the rapid and selective monitoring of target microorganisms in the environment.

Fabrication and simulation of surface plasmon resonance (SPR)-based tapered fiber sensor for E. coli detection

2018 ◽  
Vol 5 (6) ◽  
pp. 14177-14182
Author(s):  
Nina Siti Aminah ◽  
Putri H. Liani ◽  
Hendro ◽  
R. Hidayat ◽  
M. Djamal
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Fiber Sensor ◽  
Tapered Fiber ◽  
E Coli
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