Rapid Surface Plasmon Resonance-Based Inhibition Assay of Deoxynivalenol

2003 ◽  
Vol 51 (20) ◽  
pp. 5843-5848 ◽  
Author(s):  
Anna J. Tüdös ◽  
Elly R. Lucas-van den Bos ◽  
Edwin C. A. Stigter
Sensors ◽  
2011 ◽  
Vol 11 (3) ◽  
pp. 2728-2739 ◽  
Author(s):  
Yixian Wang ◽  
Zunzhong Ye ◽  
Chengyan Si ◽  
Yibin Ying

2008 ◽  
Vol 42 (6-7) ◽  
pp. 1693-1699 ◽  
Author(s):  
Chang Duk Kang ◽  
Cuong Cao ◽  
Jeewon Lee ◽  
Insung S. Choi ◽  
Byung Woo Kim ◽  
...  

2019 ◽  
Vol 174 ◽  
pp. 569-574 ◽  
Author(s):  
Zainia Rehmat ◽  
Waleed S. Mohammed ◽  
Muhammad Bilal Sadiq ◽  
Manjunath Somarapalli ◽  
Anil Kumar Anal

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Noor Azlina Masdor ◽  
Zeynep Altintas ◽  
Mohd. Yunus Shukor ◽  
Ibtisam E. Tothill

Abstract In this work, a subtractive inhibition assay (SIA) based on surface plasmon resonance (SPR) for the rapid detection of Campylobacter jejuni was developed. For this, rabbit polyclonal antibody with specificity to C. jejuni was first mixed with C. jejuni cells and unbound antibody was subsequently separated using a sequential process of centrifugation and then detected using an immobilized goat anti-rabbit IgG polyclonal antibody on the SPR sensor chip. This SIA-SPR method showed excellent sensitivity for C. jejuni with a limit of detection (LOD) of 131 ± 4 CFU mL−1 and a 95% confidence interval from 122 to 140 CFU mL−1. The method has also high specificity. The developed method showed low cross-reactivity to bacterial pathogens such as Salmonellaenterica serovar Typhimurium (7.8%), Listeria monocytogenes (3.88%) and Escherichia coli (1.56%). The SIA-SPR method together with the culturing (plating) method was able to detect C. jejuni in the real chicken sample at less than 500 CFU mL−1, the minimum infectious dose for C. jejuni while a commercial ELISA kit was unable to detect the bacterium. Since the currently available detection tools rely on culturing methods, which take more than 48 hours to detect the bacterium, the developed method in this work has the potential to be a rapid and sensitive detection method for C. jejuni.


2020 ◽  
pp. 44-49
Author(s):  
I. N. Pavlov

Two optical methods, namely surface plasmon resonance imaging and frustrated total internal reflection, are described in the paper in terms of comparing their sensitivity to change of refractive index of a thin boundary layer of an investigated medium. It is shown that, despite the fact that the theoretically calculated sensitivity is higher for the frustrated total internal reflection method, and the fact that usually in practice the surface plasmon resonance method, on the contrary, is considered more sensitive, under the same experimental conditions both methods show a similar result.


2010 ◽  
Vol 130 (7) ◽  
pp. 269-274 ◽  
Author(s):  
Takeshi Onodera ◽  
Takuzo Shimizu ◽  
Norio Miura ◽  
Kiyoshi Matsumoto ◽  
Kiyoshi Toko

PIERS Online ◽  
2008 ◽  
Vol 4 (7) ◽  
pp. 746-750 ◽  
Author(s):  
Bing-Hung Chen ◽  
Yih-Chau Wang ◽  
Jia-Hng Lin

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