scholarly journals An Airborne Multispectral Imaging System Based on Two Consumer-Grade Cameras for Agricultural Remote Sensing

2014 ◽  
Vol 6 (6) ◽  
pp. 5257-5278 ◽  
Author(s):  
Chenghai Yang ◽  
John Westbrook ◽  
Charles Suh ◽  
Daniel Martin ◽  
W. Hoffmann ◽  
...  
Keyword(s):  
Remote Sensing ◽  
Multispectral Imaging ◽  
Imaging System

Multispectral imaging system on tethered balloons for optical remote sensing education and outreach

2012 ◽  
Author(s):  
Joseph A. Shaw ◽  
Paul W. Nugent ◽  
Nathan Kaufman ◽  
Nathan J. Pust ◽  
Devin Mikes ◽  
...  
Keyword(s):  
Remote Sensing ◽  
Multispectral Imaging ◽  
Imaging System ◽  
Optical Remote Sensing ◽  
Education And Outreach

scholarly journals Design of a Tunable Snapshot Multispectral Imaging System through Ray Tracing Simulation

2019 ◽  
Vol 5 (1) ◽  
pp. 9
Author(s):  
Mengjia Ding ◽  
Peter WT Yuen ◽  
Jonathan Piper ◽  
Peter Godfree ◽  
Ayan Chatterjee ◽  
...  
Keyword(s):  
Remote Sensing ◽  
Ray Tracing ◽  
Multispectral Imaging ◽  
Imaging System ◽  
Imaging Techniques ◽  
Air Gap ◽  
Coded Aperture ◽  
Practical Usefulness ◽  
Present Design ◽  
Pros And Cons

Research on snapshot multispectral imaging has been popular in the remote sensing community due to the high demands of video-rate remote sensing system for various applications. Existing snapshot multispectral imaging techniques are mainly of a fixed wavelength type, which limits their practical usefulness. This paper describes a tunable multispectral snapshot system by using a dual prism assembly as the dispersion element of the coded aperture snapshot spectral imagers (CASSI). Spectral tuning is achieved by adjusting the air gap displacement of the dual prism assembly. Typical spectral shifts of about 1 nm at 400 nm and 12 nm at 700 nm wavelength have been achieved in the present design when the air-gap of the dual prism is changed from 4.24 mm to 5.04 mm. The paper outlines the optical designs, the performance, and the pros and cons of the dual-prism CASSI (DP-CASSI) system. The performance of the system is illustrated by TraceProTM ray tracing, to allow researchers in the field to repeat or to validate the results presented in this paper.

scholarly journals Development of a VNIR/SWIR Multispectral Imaging System for Vegetation Monitoring with Unmanned Aerial Vehicles

Sensors ◽  
2019 ◽  
Vol 19 (24) ◽  
pp. 5507 ◽  
Author(s):  
Alexander Jenal ◽  
Georg Bareth ◽  
Andreas Bolten ◽  
Caspar Kneer ◽  
Immanuel Weber ◽  
...  
Keyword(s):  
Remote Sensing ◽  
Unmanned Aerial Vehicles ◽  
Multispectral Imaging ◽  
Imaging System ◽  
Short Wave ◽  
Vegetation Monitoring ◽  
Sensing Applications ◽  
Aerial Vehicles ◽  
Remote Sensing Applications ◽  
Short Wave Infrared

Short-wave infrared (SWIR) imaging systems with unmanned aerial vehicles (UAVs) are rarely used for remote sensing applications, like for vegetation monitoring. The reasons are that in the past, sensor systems covering the SWIR range were too expensive, too heavy, or not performing well enough, as, in contrast, it is the case in the visible and near-infrared range (VNIR). Therefore, our main objective is the development of a novel modular two-channel multispectral imaging system with a broad spectral sensitivity from the visible to the short-wave infrared spectrum (approx. 400 nm to 1700 nm) that is compact, lightweight and energy-efficient enough for UAV-based remote sensing applications. Various established vegetation indices (VIs) for mapping vegetation traits can then be set up by selecting any suitable filter combination. The study describes the selection of the individual components, starting with suitable camera modules, the optical as well as the control and storage parts. Special bandpass filters are used to select the desired wavelengths to be captured. A unique flange system has been developed, which also allows the filters to be interchanged quickly in order to adapt the system to a new application in a short time. The characterization of the system was performed in the laboratory with an integrating sphere and a climatic chamber. Finally, the integration of the novel modular VNIR/SWIR imaging system into a UAV and a subsequent first outdoor test flight, in which the functionality was tested, are described.

747 Evaluation of immune microenvironment of primary lung cancer and synchronous liver metastasis with multispectral imaging system

2020 ◽  
Vol 8 (Suppl 3) ◽  
pp. A795-A795
Author(s):  
Hyeonbin Cho ◽  
Jae-Hwan Kim ◽  
Ji-Hyun Kim
Keyword(s):  
T Cells ◽  
Liver Metastasis ◽  
Multispectral Imaging ◽  
Immune Cell ◽  
Imaging System ◽  
Cytotoxic T Cells ◽  
Primary Lung Cancer ◽  
Helper T Cells ◽  
Synchronous Liver Metastasis ◽  
Immune Microenvironment

BackgroundCancer immunotherapy (CIT) has substantially improved the survival of cancer patients. However, according to recent studies, liver metastasis was reported to predict worse outcomes for CIT. The main objective of the study is to evaluate the differences in the immune microenvironment (IME) between the primary lung cancer (PL) and synchronous liver metastasis (LM) using a multispectral imaging system.MethodsSix immune markers (CD4, CD8, CTLA-4, granzyme B (GZB), Foxp3 and PD-L1) were analyzed using a multiplex IHC system and inForm program (Akoya) on paired lung-liver samples of 10 patients. Cells were categorized into tumor nest and stroma, and cell counts per unit area were measured for comparison.ResultsThe number of tumor-infiltrating cytotoxic T cells (TIL) in PL (262.5 cells/mm2) was higher than that of LM (113.3 cells/mm2). Additionally, the ratio between the number of TIL and non-TIL was greater in PL (0.31) compared to that of LM (0.26). A similar trend appeared for Helper T cells and regulatory T cells (Treg), as PL consisted of higher numbers of T cells (791.8 Helper T cells/mm2, 195.7 Treg/mm2) than LM (626.3 Helper T cells/mm2, 121.3 Treg/mm2). However, cytotoxic T cells exhibiting GZB+ and CTLA-4- were fewer in PL (140.2 cells/mm2) than in LM (203.3 cells/mm2), and the ratio is 0.69. The mean number of GZB+ TIL in PL (32.5 cells/mm2) was lower than in LM (35.3 cells/mm2), and their proportions among total TIL counts were 0.12 and 0.31, respectively. In PL, GZB+: GZB- ratio is 0.16 while the ratio is 1.91 for LM. A fewer number of TILs exhibiting GZB suggests that PL has lower efficiency in immune response than LM. Another crucial checkpoint receptor that inhibits immune response, CTLA-4, was more prevalent in PL, with CTLA-4+: CTLA-4- ratio in Treg being 0.36 in PL, compared to 0.11 in LM. The tumor proportion score (TPS) of PD-L1 was higher in PL than LM (40.0 vs. 6.6).ConclusionsIn our study, we showed the differences in the numbers of TIL or regulatory T cells and expressions of immune checkpoint receptors (PD-L1, CTLA-4), which significantly influence outcomes for CIT. The study is ongoing to confirm different IME between the PL and LM groups in a larger tumor cohort.ReferencesPeng, Jianhong, et al., Immune Cell Infiltration in the Microenvironment of Liver Oligometastasis from Colorectal Cancer: Intratumoural CD8/CD3 Ratio Is a Valuable Prognostic Index for Patients Undergoing Liver Metastasectomy. Cancers 2019 Dec; 11(12): 1922. https://doi.org/10.3390/cancers11121922Tumeh, Paul C., et al., Liver Metastasis and treatment outcome with Anti-PD-1 monoclonal antibody in patients with melanoma and NSCLC. Cancer Immunol Res 2017 May; 5(5): 417–424. doi: 10.1158/2326-6066.CIR-16-0325Parra, E.R., Immune Cell Profiling in Cancer Using Multiplex Immunofluorescence and Digital Analysis Approaches; Streckfus, C.F., Ed.; IntechOpen: London, UK, 2018; pp. 1–13. doi: 10.5772/intechopen.80380Ribas, A., Hu-Lieskovan, S., What does PD-L1 positive or negative mean?. The Journal of Experimental Medicine 2016;213(13):2835–2840. https://doi.org/10.1084/jem.20161462

scholarly journals Optimized Method for Mapping Inorganic Pigments by Means of Multispectral Imaging Combined with Hyperspectral Spectroscopy for the Study of Vincenzo Pasqualoni’s Wall Painting at the Basilica of S. Nicola in Carcere in Rome

Minerals ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 839
Author(s):  
Lucilla Pronti ◽  
Giuseppe Capobianco ◽  
Margherita Vendittelli ◽  
Anna Candida Felici ◽  
Silvia Serranti ◽  
...  
Keyword(s):  
Multispectral Imaging ◽  
Imaging System ◽  
Principal Component ◽  
Reflectance Spectroscopy ◽  
Wall Painting ◽  
High Spectral Resolution ◽  
Spectral Difference ◽  
Color Palette ◽  
A Minor ◽  
Nir Reflectance Spectroscopy

Multispectral imaging is a preliminary screening technique for the study of paintings. Although it permits the identification of several mineral pigments by their spectral behavior, it is considered less performing concerning hyperspectral imaging, since a limited number of wavelengths are selected. In this work, we propose an optimized method to map the distribution of the mineral pigments used by Vincenzo Pasqualoni for his wall painting placed at the Basilica of S. Nicola in Carcere in Rome, combining UV/VIS/NIR reflectance spectroscopy and multispectral imaging. The first method (UV/VIS/NIR reflectance spectroscopy) allowed us to characterize pigment layers with a high spectral resolution; the second method (UV/VIS/NIR multispectral imaging) permitted the evaluation of the pigment distribution by utilizing a restricted number of wavelengths. Combining the results obtained from both devices was possible to obtain a distribution map of a pictorial layer with a high accuracy level of pigment recognition. The method involved the joint use of point-by-point hyperspectral spectroscopy and Principal Component Analysis (PCA) to identify the pigments in the color palette and evaluate the possibility to discriminate all the pigments recognized, using a minor number of wavelengths acquired through the multispectral imaging system. Finally, the distribution and the spectral difference of the different pigments recognized in the multispectral images, (in this case: red ochre, yellow ochre, orpiment, cobalt blue-based pigments, ultramarine and chrome green) were shown through PCA false-color images.

scholarly journals Characterizing the Tumor Immune Microenvironment with Tyramide-Based Multiplex Immunofluorescence

2020 ◽  
Vol 25 (4) ◽  
pp. 417-432
Author(s):  
Hidetoshi Mori ◽  
Jennifer Bolen ◽  
Louis Schuetter ◽  
Pierre Massion ◽  
Clifford C. Hoyt ◽  
...  
Keyword(s):  
Tumor Microenvironment ◽  
Multispectral Imaging ◽  
Imaging System ◽  
Predictive Biomarkers ◽  
Cell Types ◽  
Immune Microenvironment ◽  
Tumor Immune Microenvironment ◽  
Manual Processing ◽  
Cell Densities ◽  
Immune Profiling

AbstractMultiplex immunofluorescence (mIF) allows simultaneous antibody-based detection of multiple markers with a nuclear counterstain on a single tissue section. Recent studies have demonstrated that mIF is becoming an important tool for immune profiling the tumor microenvironment, further advancing our understanding of the interplay between cancer and the immune system, and identifying predictive biomarkers of response to immunotherapy. Expediting mIF discoveries is leading to improved diagnostic panels, whereas it is important that mIF protocols be standardized to facilitate their transition into clinical use. Manual processing of sections for mIF is time consuming and a potential source of variability across numerous samples. To increase reproducibility and throughput we demonstrate the use of an automated slide stainer for mIF incorporating tyramide signal amplification (TSA). We describe two panels aimed at characterizing the tumor immune microenvironment. Panel 1 included CD3, CD20, CD117, FOXP3, Ki67, pancytokeratins (CK), and DAPI, and Panel 2 included CD3, CD8, CD68, PD-1, PD-L1, CK, and DAPI. Primary antibodies were first tested by standard immunohistochemistry and single-plex IF, then multiplex panels were developed and images were obtained using a Vectra 3.0 multispectral imaging system. Various methods for image analysis (identifying cell types, determining cell densities, characterizing cell-cell associations) are outlined. These mIF protocols will be invaluable tools for immune profiling the tumor microenvironment.

Development of a Controller Unit for Multispectral Imaging System

2020 ◽  
Author(s):  
Mate Koba ◽  
Attila Trohak ◽  
Norbert Zajzon ◽  
Richard Zoltan Papp ◽  
Marton L. Kiss
Keyword(s):  
Multispectral Imaging ◽  
Imaging System
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