scholarly journals L-(+)-Lactic Acid from Reed: Comparing Various Resources for the Nutrient Provision of B. coagulans

Resources ◽  
2020 ◽  
Vol 9 (7) ◽  
pp. 89 ◽  
Author(s):  
Linda Schroedter ◽  
Roland Schneider ◽  
Lisa Remus ◽  
Joachim Venus
Keyword(s):  
Lactic Acid ◽  
First Generation ◽  
Low Cost ◽  
Optical Purity ◽  
Value Added ◽  
Bacillus Coagulans ◽  
Common Reed ◽  
Biotechnological Production ◽  
Food And Feed ◽  
Pentose Sugars

Biotechnological production of lactic acid (LA) is based on the so-called first generation feedstocks, meaning sugars derived from food and feed crops such as corn, sugarcane and cassava. The aim of this study was to exploit the potential of a second generation resource: Common reed (Phragmites australis) is a powerfully reproducing sweet grass which grows in wetlands and creates vast monocultural populations. This lignocellulose biomass bears the possibility to be refined to value-added products, without competing with agro industrial land. Besides utilizing reed as a renewable and inexpensive substrate, low-cost nutritional supplementation was analyzed for the fermentation of thermophilic Bacillus coagulans. Various nutritional sources such as baker’s and brewer’s yeast, lucerne green juice and tryptone were investigated for the replacement of yeast extract. The structure of the lignocellulosic material was tackled by chemical treatment (1% NaOH) and enzymatic hydrolysis (Cellic® CTec2). B. coagulans DSM ID 14-300 was employed for the homofermentative conversion of the released hexose and pentose sugars to polymerizable L-(+)-LA of over 99.5% optical purity. The addition of autolyzed baker’s yeast led to the best results of fermentation, enabling an LA titer of 28.3 g L−1 and a yield of 91.6%.

scholarly journals Use of wheat straw and chicken feather hydrolysates as a complete medium for lactic acid production

2018 ◽  
Vol 36 (No. 2) ◽  
pp. 146-153 ◽  
Author(s):  
Gharwalová Lucia ◽  
Paulová Leona ◽  
Patáková Petra ◽  
Branská Barbora ◽  
Melzoch Karel
Keyword(s):  
Lactic Acid ◽  
Wheat Straw ◽  
Acid Production ◽  
Low Cost ◽  
Lactic Acid Production ◽  
Nitrogen Sources ◽  
Batch Process ◽  
Complete Medium ◽  
Biotechnological Production ◽  
Acid Yield

Biotechnological production of lactic acid has experienced a boom that is hindered only by the lack of low-cost, abundant material that might be used as a substrate for lactic acid bacteria. Such material should contain not only carbon but also complex nitrogen sources, amino acids and vitamins necessary for the balanced growth of the bacteria. Here, for the first time, a combination of hydrolysates of wheat straw and chicken feathers was used as a complete waste cultivation medium for lactic acid production. It was shown to be a promising substrate for lactic acid production, reducing the medium price by 73% compared with MRS broth, providing more than 98% lactic acid yield and high productivity (2.28 ± 0.68 g/l/h) in a fed-batch process using Lactobacillus reuterii LHR14.

scholarly journals Kaskadennutzung von Lignocellulose: LX-Verfahren trifft auf B. coagulans

BIOspektrum ◽  
2020 ◽  
Vol 26 (7) ◽  
pp. 803-805
Author(s):  
Linda Schroedter ◽  
Friedrich Streffer ◽  
Katrin Streffer ◽  
Peter Unger ◽  
Joachim Venus
Keyword(s):  
Lactic Acid ◽  
Energy Saving ◽  
Optical Purity ◽  
Bacillus Coagulans ◽  
The Novel ◽  
Corn Straw

AbstractInvestigating alternatives for petrobased substrates, lignocellulose is an interesting yet complex feedstock that offers various possibilities for the design of new and sustainable chemical routes. The novel energy-saving LX-pretreatment was combined with thermophilic Bacillus coagulans. By this, corn straw was used in an innovative cascade obtaining biogas, lignin as well as polymerisable L-(+)-lactic acid of over 99 percents optical purity.

scholarly journals Production and Purification of l-lactic Acid in Lab and Pilot Scales Using Sweet Sorghum Juice

Fermentation ◽  
2019 ◽  
Vol 5 (2) ◽  
pp. 36 ◽  
Author(s):  
Agata Olszewska-Widdrat ◽  
Maria Alexandri ◽  
José Pablo López-Gómez ◽  
Roland Schneider ◽  
Michael Mandl ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Sweet Sorghum ◽  
Lactic Acid Production ◽  
Optical Purity ◽  
Bacillus Coagulans ◽  
Pilot Scale ◽  
Environmentally Benign ◽  
Downstream Process ◽  
Sweet Sorghum Juice ◽  
Ultra Filtration

Sweet sorghum juice (SSJ) was evaluated as fermentation substrate for the production of l-lactic acid. A thermophilic Bacillus coagulans isolate was selected for batch fermentations without the use of additional nutrients. The first batch of SSJ (Batch A) resulted on higher lactic acid concentration, yield and productivity with values of 78.75 g∙L−1, 0.78 g∙g−1 and 1.77 g∙L−1 h−1, respectively. Similar results were obtained when the process was transferred into the pilot scale (50 L), with corresponding values of 73 g∙L−1, 0.70 g∙g−1 and 1.47 g∙L−1 h−1. A complete downstream process scheme was developed in order to separate lactic acid from the fermentation components. Coarse and ultra-filtration were employed as preliminary separation steps. Mono- and bipolar electrodialysis, followed by chromatography and vacuum evaporation were subsequently carried out leading to a solution containing 905.8 g∙L−1 lactic acid, with an optical purity of 98.9%. The results of this study highlight the importance of the downstream process with respect to using SSJ for lactic acid production. The proposed downstream process constitutes a more environmentally benign approach to conventional precipitation methods.

scholarly journals Major Role of NAD-Dependent Lactate Dehydrogenases in the Production of l-Lactic Acid with High Optical Purity by the Thermophile Bacillus coagulans

2014 ◽  
Vol 80 (23) ◽  
pp. 7134-7141 ◽  
Author(s):  
Limin Wang ◽  
Yumeng Cai ◽  
Lingfeng Zhu ◽  
Honglian Guo ◽  
Bo Yu
Keyword(s):  
Lactic Acid ◽  
Lactate Dehydrogenase ◽  
Acid Production ◽  
Lactic Acid Production ◽  
Optical Purity ◽  
Bacillus Coagulans ◽  
Lactobacillus Delbrueckii ◽  
Content Type ◽  
High Optical Purity ◽  
Optically Pure

ABSTRACTBacillus coagulans2-6 is an excellent producer of optically purel-lactic acid. However, little is known about the mechanism of synthesis of the highly optically purel-lactic acid produced by this strain. Three enzymes responsible for lactic acid production—NAD-dependentl-lactate dehydrogenase (l-nLDH; encoded byldhL), NAD-dependentd-lactate dehydrogenase (d-nLDH; encoded byldhD), and glycolate oxidase (GOX)—were systematically investigated in order to study the relationship between these enzymes and the optical purity of lactic acid.Lactobacillus delbrueckiisubsp.bulgaricusDSM 20081 (ad-lactic acid producer) andLactobacillus plantarumsubsp.plantarumDSM 20174 (adl-lactic acid producer) were also examined in this study as comparative strains, in addition toB. coagulans. The specific activities of key enzymes for lactic acid production in the three strains were characterizedin vivoandin vitro, and the levels of transcription of theldhL,ldhD, and GOX genes during fermentation were also analyzed. The catalytic activities ofl-nLDH andd-nLDH were different inl-,d-, anddl-lactic acid producers. Onlyl-nLDH activity was detected inB. coagulans2-6 under native conditions, and the level of transcription ofldhLinB. coagulans2-6 was much higher than that ofldhDor the GOX gene at all growth phases. However, for the twoLactobacillusstrains used in this study,ldhDtranscription levels were higher than those ofldhL. The high catalytic efficiency ofl-nLDH toward pyruvate and the high transcription ratios ofldhLtoldhDandldhLto the GOX gene provide the key explanations for the high optical purity ofl-lactic acid produced byB. coagulans2-6.

scholarly journals Genome Sequence of the Thermophilic Strain Bacillus coagulans XZL4, an Efficient Pentose-Utilizing Producer of Chemicals

2011 ◽  
Vol 193 (22) ◽  
pp. 6398-6399 ◽  
Author(s):  
Fei Su ◽  
Ke Xu ◽  
Bo Zhao ◽  
Cui Tai ◽  
Fei Tao ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Carbohydrate Metabolism ◽  
Genome Sequence ◽  
Bacillus Coagulans ◽  
Content Type ◽  
Pentose Sugars

Bacillus coagulans XZL4 is an efficient pentose-utilizing producer of important platform compounds, such as l -lactic acid, 2,3-butanediol, and acetoin. Here we present a 2.8-Mb assembly of its genome. Simple and efficient carbohydrate metabolism systems, especially the transketolase/transaldolase pathway, make it possible to convert pentose sugars to products at high levels.

Sugar beet syrups in lactic acid fermentation – Part I

2014 ◽  
pp. 495-502 ◽  
Author(s):  
Timo J. Koch ◽  
Joachim Venus ◽  
Martin Bruhns
Keyword(s):  
Lactic Acid ◽  
Sugar Beet ◽  
Corn Starch ◽  
Lactic Acid Production ◽  
Optical Purity ◽  
Acid Fermentation ◽  
Biotechnological Production ◽  
Technical Application ◽  
General Evaluation ◽  
Thick Juice

Biotechnological production of lactic acid has been studied in various ways, e.g. microorganisms, fermentation processes, down-stream processes, fermentation substrates, and fermentation nutrients. The problems for all processes still are high costs for feedstock and fermentation nutrients. The objective of this study is a general evaluation of sugar beet thick juice from Pfeifer & Langen GmbH & Co. KG, Germany as a substrate for lactic acid production. In a series of fermentation experiments the results based on thick juice were comparable to those obtained using cane raw sugar and even better than using conventional corn starch as a fermentation subtrate. The most important findings for a later technical application are the high volumetric productivity (up to 5.5g·L–1·h–1), and the optical purity of the lactic acid (>99% ee l-LA).

scholarly journals Simultaneous Bioconversion of Gelatinized Starchy Waste from the Rice Noodle Manufacturing Process to Lactic Acid and Maltose-Forming α-Amylase by Lactobacillus plantarum S21, Using a Low-Cost Medium

Fermentation ◽  
2019 ◽  
Vol 5 (2) ◽  
pp. 32 ◽  
Author(s):  
Kridsada Unban ◽  
Apinun Kanpiengjai ◽  
Nuttapong Khatthongngam ◽  
Chalermpong Saenjum ◽  
Chartchai Khanongnuch
Keyword(s):  
Lactic Acid ◽  
Lactobacillus Plantarum ◽  
Low Cost ◽  
Corn Steep Liquor ◽  
Lactic Acid Production ◽  
Value Added ◽  
Tween 80 ◽  
Industrial Wastes ◽  
Acid Yield ◽  
Medium Components

A direct bioconversion of gelatinized starchy waste (GSW) to lactic acid by amylolytic lactic acid bacterium Lactobacillus plantarum S21 was investigated. Corn steep liquor (CSL) was selected as the most suitable low-cost nitrogen source for replacing yeast extract, beef extract, and peptone in De Man, Rogosa and Sharpe (MRS) medium. Plackett–Burman design results indicated that GSW and CSL were the two most nutrients that significantly influence lactic acid production, among eight medium components, including GSW, CSL, K2HPO4, CH3COONa, (NH4)2HC6H5O7, MgSO4, MnSO4, and Tween 80. A new low-cost medium containing only GSW (134.4 g/L) and CSL (187.7 g/L) was achieved as omitting other six components from the optimized medium had no effect on lactic acid yield. Batch fermentation at 37 °C both in 1 L and 10 L jar fermenters showed non-significantly different productivity. A by-product, maltose-forming α-amylase, was successfully achieved up to 96% recovery yield using an ultrafiltration unit equipped with a 50 kDa cut-off membrane. Crude lactic acid exhibited the additional benefit of antimicrobial activity against food and feed pathogens Salmonella enterica serovar Typhimurium TISTR 292, Vibrio cholerae TH-001, and also E. coli ATCC 25922. This study presents a promising bioprocess for the simultaneous production of lactic acid, and a value-added food enzyme, using only two industrial wastes, GSW and CSL, as the medium components.

scholarly journals Efficient lactic acid production from dilute acid-pretreated lignocellulosic biomass by a synthetic consortium of engineered Pseudomonas putida and Bacillus coagulans

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Lihua Zou ◽  
Shuiping Ouyang ◽  
Yueli Hu ◽  
Zhaojuan Zheng ◽  
Jia Ouyang
Keyword(s):  
Lactic Acid ◽  
Pseudomonas Putida ◽  
Lignocellulosic Biomass ◽  
Acid Production ◽  
Levulinic Acid ◽  
Lactic Acid Production ◽  
Value Added ◽  
Bacillus Coagulans ◽  
Lignocellulosic Hydrolysate ◽  
Acid Yield

Abstract Background Lignocellulosic biomass is an attractive and sustainable alternative to petroleum-based feedstock for the production of a range of biochemicals, and pretreatment is generally regarded as indispensable for its biorefinery. However, various inhibitors that severely hinder the growth and fermentation of microorganisms are inevitably produced during the pretreatment of lignocellulose. Presently, there are few reports on a single microorganism that can detoxify or tolerate toxic mixtures of pretreated lignocellulose hydrolysate while effectively transforming sugar components into valuable compounds. Alternatively, microbial coculture provides a simpler and more efficacious way to realize this goal by distributing metabolic functions among different specialized strains. Results In this study, a novel synthetic microbial consortium, which is composed of a responsible for detoxification bacterium engineered Pseudomonas putida KT2440 and a lactic acid production specialist Bacillus coagulans NL01, was developed to directly produce lactic acid from highly toxic lignocellulosic hydrolysate. The engineered P. putida with deletion of the sugar metabolism pathway was unable to consume the major fermentable sugars of lignocellulosic hydrolysate but exhibited great tolerance to 10 g/L sodium acetate, 5 g/L levulinic acid, 10 mM furfural and HMF as well as 2 g/L monophenol compound. In addition, the engineered strain rapidly removed diverse inhibitors of real hydrolysate. The degradation rate of organic acids (acetate, levulinic acid) and the conversion rate of furan aldehyde were both 100%, and the removal rate of most monoaromatic compounds remained at approximately 90%. With detoxification using engineered P. putida for 24 h, the 30% (v/v) hydrolysate was fermented to 35.8 g/L lactic acid by B. coagulans with a lactic acid yield of 0.8 g/g total sugars. Compared with that of the single culture of B. coagulans without lactic acid production, the fermentation performance of microbial coculture was significantly improved. Conclusions The microbial coculture system constructed in this study demonstrated the strong potential of the process for the biosynthesis of valuable products from lignocellulosic hydrolysates containing high concentrations of complex inhibitors by specifically recruiting consortia of robust microorganisms with desirable characteristics and also provided a feasible and attractive method for the bioconversion of lignocellulosic biomass to other value-added biochemicals.

scholarly journals Efficient Lactic Acid Production from Dilute Acid Pretreated Lignocellulosic Biomass by a Synthetic Consortia of Engineered Pseudomonas Putida and Bacillus Coagulans

2021 ◽  
Author(s):  
Lihua Zou ◽  
Shuiping Ouyang ◽  
Yueli Hu ◽  
Zhaojuan Zheng ◽  
Jia Ouyang
Keyword(s):  
Lactic Acid ◽  
Pseudomonas Putida ◽  
Lignocellulosic Biomass ◽  
Acid Production ◽  
Lactic Acid Production ◽  
Value Added ◽  
Bacillus Coagulans ◽  
Microbial Consortia ◽  
Lignocellulosic Hydrolysate ◽  
High Concentration

Abstract Background Lignocellulosic biomass is an attractive and sustainable alternative to petroleum-based feedstock for the production a range of biochemicals, and a pretreatment is generally regarded to be indispensable for its bio-refinery. Nevertheless, various inhibitors that severely hindered the growth and fermentation of microorganisms were produced inevitably during the pretreatment of lignocellulose. Presently, a single microorganism that can tolerate toxic mixtures of pretreatment hydrolysate while effectively transforming sugar components into valuable compound is less well reported. Alternatively, microbial co-culture provides a simpler and more efficacious way to realize this goal via distributing metabolic tasks among proper strains. Results In this study, a novel synthetic microbial consortia, which is composed of a responsible for detoxification bacterium engineered Pseudomonas putida KT2440 and a lactic acid production specialist Bacillus coagulans NL01, was developed to directly produce lactic acid from high-toxic lignocellulosic hydrolysate. The engineered P. putida with deletion of sugar metabolism pathway was suggested to be unable to consume the major fermentable sugars of lignocellulosic hydrolysate, but can rapidly remove inhibitors in hydrolysate. With detoxification using engineered P. putida for 24 h, the pretreated hydrolysate was fermented into 35.8 g/L of lactic acid by B. coagulans with a yield of 90%. The fermentation performance of microbial co-culture was significantly improved than that single culture of B. coagulans without lactic acid production. Conclusions The microbial coculture system constructed by this study demonstrated strong potential of the process for biosynthesis of valuable product from lignocellulosic hydrolysate containing high concentration of complex inhibitors by specifically recruited consortia of robust microorganisms with desirable characteristics and also provided a feasible and attractive method for bioconversion of lignocellulosic biomass to other value-added biochemicals.

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