scholarly journals Simultaneous Bioconversion of Gelatinized Starchy Waste from the Rice Noodle Manufacturing Process to Lactic Acid and Maltose-Forming α-Amylase by Lactobacillus plantarum S21, Using a Low-Cost Medium

Fermentation ◽  
2019 ◽  
Vol 5 (2) ◽  
pp. 32 ◽  
Author(s):  
Kridsada Unban ◽  
Apinun Kanpiengjai ◽  
Nuttapong Khatthongngam ◽  
Chalermpong Saenjum ◽  
Chartchai Khanongnuch
Keyword(s):  
Lactic Acid ◽  
Lactobacillus Plantarum ◽  
Low Cost ◽  
Corn Steep Liquor ◽  
Lactic Acid Production ◽  
Value Added ◽  
Tween 80 ◽  
Industrial Wastes ◽  
Acid Yield ◽  
Medium Components

A direct bioconversion of gelatinized starchy waste (GSW) to lactic acid by amylolytic lactic acid bacterium Lactobacillus plantarum S21 was investigated. Corn steep liquor (CSL) was selected as the most suitable low-cost nitrogen source for replacing yeast extract, beef extract, and peptone in De Man, Rogosa and Sharpe (MRS) medium. Plackett–Burman design results indicated that GSW and CSL were the two most nutrients that significantly influence lactic acid production, among eight medium components, including GSW, CSL, K2HPO4, CH3COONa, (NH4)2HC6H5O7, MgSO4, MnSO4, and Tween 80. A new low-cost medium containing only GSW (134.4 g/L) and CSL (187.7 g/L) was achieved as omitting other six components from the optimized medium had no effect on lactic acid yield. Batch fermentation at 37 °C both in 1 L and 10 L jar fermenters showed non-significantly different productivity. A by-product, maltose-forming α-amylase, was successfully achieved up to 96% recovery yield using an ultrafiltration unit equipped with a 50 kDa cut-off membrane. Crude lactic acid exhibited the additional benefit of antimicrobial activity against food and feed pathogens Salmonella enterica serovar Typhimurium TISTR 292, Vibrio cholerae TH-001, and also E. coli ATCC 25922. This study presents a promising bioprocess for the simultaneous production of lactic acid, and a value-added food enzyme, using only two industrial wastes, GSW and CSL, as the medium components.

scholarly journals Use of wheat straw and chicken feather hydrolysates as a complete medium for lactic acid production

2018 ◽  
Vol 36 (No. 2) ◽  
pp. 146-153 ◽  
Author(s):  
Gharwalová Lucia ◽  
Paulová Leona ◽  
Patáková Petra ◽  
Branská Barbora ◽  
Melzoch Karel
Keyword(s):  
Lactic Acid ◽  
Wheat Straw ◽  
Acid Production ◽  
Low Cost ◽  
Lactic Acid Production ◽  
Nitrogen Sources ◽  
Batch Process ◽  
Complete Medium ◽  
Biotechnological Production ◽  
Acid Yield

Biotechnological production of lactic acid has experienced a boom that is hindered only by the lack of low-cost, abundant material that might be used as a substrate for lactic acid bacteria. Such material should contain not only carbon but also complex nitrogen sources, amino acids and vitamins necessary for the balanced growth of the bacteria. Here, for the first time, a combination of hydrolysates of wheat straw and chicken feathers was used as a complete waste cultivation medium for lactic acid production. It was shown to be a promising substrate for lactic acid production, reducing the medium price by 73% compared with MRS broth, providing more than 98% lactic acid yield and high productivity (2.28 ± 0.68 g/l/h) in a fed-batch process using Lactobacillus reuterii LHR14.

scholarly journals Efficient lactic acid production from dilute acid-pretreated lignocellulosic biomass by a synthetic consortium of engineered Pseudomonas putida and Bacillus coagulans

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Lihua Zou ◽  
Shuiping Ouyang ◽  
Yueli Hu ◽  
Zhaojuan Zheng ◽  
Jia Ouyang
Keyword(s):  
Lactic Acid ◽  
Pseudomonas Putida ◽  
Lignocellulosic Biomass ◽  
Acid Production ◽  
Levulinic Acid ◽  
Lactic Acid Production ◽  
Value Added ◽  
Bacillus Coagulans ◽  
Lignocellulosic Hydrolysate ◽  
Acid Yield

Abstract Background Lignocellulosic biomass is an attractive and sustainable alternative to petroleum-based feedstock for the production of a range of biochemicals, and pretreatment is generally regarded as indispensable for its biorefinery. However, various inhibitors that severely hinder the growth and fermentation of microorganisms are inevitably produced during the pretreatment of lignocellulose. Presently, there are few reports on a single microorganism that can detoxify or tolerate toxic mixtures of pretreated lignocellulose hydrolysate while effectively transforming sugar components into valuable compounds. Alternatively, microbial coculture provides a simpler and more efficacious way to realize this goal by distributing metabolic functions among different specialized strains. Results In this study, a novel synthetic microbial consortium, which is composed of a responsible for detoxification bacterium engineered Pseudomonas putida KT2440 and a lactic acid production specialist Bacillus coagulans NL01, was developed to directly produce lactic acid from highly toxic lignocellulosic hydrolysate. The engineered P. putida with deletion of the sugar metabolism pathway was unable to consume the major fermentable sugars of lignocellulosic hydrolysate but exhibited great tolerance to 10 g/L sodium acetate, 5 g/L levulinic acid, 10 mM furfural and HMF as well as 2 g/L monophenol compound. In addition, the engineered strain rapidly removed diverse inhibitors of real hydrolysate. The degradation rate of organic acids (acetate, levulinic acid) and the conversion rate of furan aldehyde were both 100%, and the removal rate of most monoaromatic compounds remained at approximately 90%. With detoxification using engineered P. putida for 24 h, the 30% (v/v) hydrolysate was fermented to 35.8 g/L lactic acid by B. coagulans with a lactic acid yield of 0.8 g/g total sugars. Compared with that of the single culture of B. coagulans without lactic acid production, the fermentation performance of microbial coculture was significantly improved. Conclusions The microbial coculture system constructed in this study demonstrated the strong potential of the process for the biosynthesis of valuable products from lignocellulosic hydrolysates containing high concentrations of complex inhibitors by specifically recruiting consortia of robust microorganisms with desirable characteristics and also provided a feasible and attractive method for the bioconversion of lignocellulosic biomass to other value-added biochemicals.

scholarly journals Effective D-lactic acid production from corncobs by simultaneous saccharification and fermentation using metabolically engineered Lactobacillus plantarum

2021 ◽  
Author(s):  
Jun-ichi Horiuchi ◽  
Syuka Naito ◽  
Yoichi Kumada ◽  
Kenji Okano ◽  
Akihiko Kondo ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactobacillus Plantarum ◽  
Acid Production ◽  
Simultaneous Saccharification And Fermentation ◽  
Lactic Acid Production ◽  
Enzymatic Saccharification ◽  
Optical Purity ◽  
Acid Yield ◽  
Corncob Hydrolysate ◽  
Simultaneous Saccharification

A metabolically engineered Lactobacillus plantarum mutant, which could produce D-lactic acid from both glucose and xylose, was applied for the production of optically pure D-lactic acid from corncobs by simultaneous saccharification and fermentation (SSF). Using a corncob hydrolysate obtained by a combination of dilute acid treatment using 1.5% H2SO4 followed by enzymatic saccharification, the L. plantarum mutant completely assimilated both glucose and xylose in the corncob hydrolysate within 20 hours, resulting in the successful production of D-lactic acid with high optical purity in a batch culture. To improve the performance of D-lactic acid production from corncobs, SSF experiments from 100 to 250 g/L of acid-hydrolyzed corncobs using 1.5% H2SO4 were performed, and 49.7 to 101 g/L of D-lactic acid with 96.8-98.6% of optical purity was successfully produced. The D-lactic acid yield from corncobs (YL/C) was approx. 0.61 when 100-150 g/L of acid-hydrolyzed corncobs was used; however, the YL/C decreased to 0.49 as the concentration of acid-hydrolyzed corncobs because of insufficient acid hydrolysis of the corncobs. Therefore, by increasing the H2SO4 concentration to 3.5%, D-lactic acid production from corncobs significantly increased to 134 g/L with YL/C of 0.63 and 2.88 g/(L･h) of productivity from 250 g/L of acid-hydrolyzed corncobs.

scholarly journals Improvement of Enantiomeric l-Lactic Acid Production from Mixed Hexose-Pentose Sugars by Coculture of Enterococcus mundtii WX1 and Lactobacillus rhamnosus SCJ9

Fermentation ◽  
2021 ◽  
Vol 7 (2) ◽  
pp. 95
Author(s):  
Augchararat Klongklaew ◽  
Kridsada Unban ◽  
Apinun Kanpiengjai ◽  
Pairote Wongputtisin ◽  
Punnita Pamueangmun ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Glucose Repression ◽  
Lactic Acid Production ◽  
Lactobacillus Rhamnosus ◽  
Tween 80 ◽  
Xylose Consumption ◽  
Practical Applications ◽  
Medium Components ◽  
Enterococcus Mundtii ◽  
Total Glucose

Among 39 pentose-utilizing lactic acid bacteria (LAB) selected from acid-forming bacteria from the midgut of Eri silkworm, the isolate WX1 was selected with the highest capability to produce optically pure l-lactic acid (l-LA) from glucose, xylose and arabinose with furfural-tolerant properties. The isolate WX1 was identified as Enterococcus mundtii based on 16S rDNA sequence analysis. The conversion yields of l-LA from glucose and xylose by E. mundtii WX1 were 0.97 and 0.68 g/g substrate, respectively. Furthermore, l-LA production by E. mundtii WX1 in various glucose-xylose mixtures indicated glucose repression effect on xylose consumption. The coculture of E. mundtii WX1 and Lactobacillus rhamnosus SCJ9, a homofermentative LAB capable of producing l-LA from glucose clearly showed an improvement of l-LA production from 30 g/L total glucose-xylose (6:4). The results from Plackett–Burman design (PBD) indicated that Tween 80, MnSO4 and yeast extract (YE) were three medium components that significantly influenced (p < 0.05) l-LA production using the coculture strategy in the presence of 2 g/L furfural. Optimal concentrations of these variables revealed by central composite design (CCD) and response surface methodology (RSM) were 20.61 g/L YE, 1.44 g/L Tween 80 and 1.27 g/L MnSO4. Based on the optimized medium with 30 g/L total glucose-xylose (6:4), the maximum experimental l-LA value of 23.59 g/L reflecting 0.76 g/g substrate were achieved from 48 h fermentation at 37 °C. l-LA produced by coculture cultivated under standard MRS medium and new optimized conditions were 1.28 and 1.53 times higher than that obtained from single culture by E. mundtii WX1, respectively. This study provides the foundations for practical applications of coculture in bioconversion of lignocellulose particularly glucose-xylose-rich corn stover to l-LA.

scholarly journals Evaluation of biological production of lactic acid in a synthetic medium and in Aloe vera (L.) Burm. f. processing by-products

2015 ◽  
Vol 20 (3) ◽  
pp. 369 ◽  
Author(s):  
Javier Antonio Gómez-Gómez ◽  
Catalina Giraldo-Estrada ◽  
David Habeych ◽  
Sandra Baena
Keyword(s):  
Lactic Acid ◽  
Acid Production ◽  
Low Cost ◽  
Lactic Acid Production ◽  
Synthetic Medium ◽  
Aloe Vera ◽  
Acid Yield ◽  
Growing Conditions ◽  
Defined Culture ◽  
By Products

This study evaluated lactic acid production through batch fermentation in a bioreactor with <em>Thermoanaerobacter</em> sp. strain USBA-018 and a chemically defined culture medium and with hydrolyzed pressed extract of <em>Aloe vera</em> peel (AHE). The strain USBA-018 fermented various sugars, but its primary end-product was L-lactic acid. Factors which influenced L- lactic acid production were pH, addition of yeast extract (YE) and manganese chloride. Under the most favorable growing conditions for the production of lactic acid, yield (Yp/s) increased from 0.66 to 0.96 g/g with a productivity (Qp) of 0.62 g.l-1.h and a maximum lactic acid concentration of 178 mM at 26 hours of fermentation. When AHE was used, 93.3 mM, or 0.175 g.h/L, was obtained. These results show the potential for transformation of sugars that strain USBA-018 offers, but additional studies are needed to find out if different strategies using AHE as carbon source can produce large enough quantities of lactic acid to allow AHE to become a low-cost alternative substrate.

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