scholarly journals Characterization of Grain Amaranth (Amaranthus spp.) Germplasm in South West Nigeria Using Morphological, Nutritional, and Random Amplified Polymorphic DNA (RAPD) Analysis

Resources ◽  
2016 ◽  
Vol 5 (1) ◽  
pp. 6 ◽  
Author(s):  
Pamela Akin-Idowu ◽  
Michael Gbadegesin ◽  
Uterdzua Orkpeh ◽  
Dorcas Ibitoye ◽  
Oyeronke Odunola
Keyword(s):  
Rapd Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Grain Amaranth ◽  
South West ◽  
Amaranthus Spp

scholarly journals 678 PB 186 RAPD GENETIC MARKERS FOR CHARACTERIZATION OF MUSA GENETIC RESOURCES

HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 530a-530
Author(s):  
R.L. Jarret ◽  
K.V. Bhat
Keyword(s):  
Genetic Markers ◽  
Size Range ◽  
Rapd Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Dna Amplification ◽  
Principal Coordinate Analysis ◽  
Phenetic Analysis ◽  
Similarity Coefficients ◽  
Primer Sequence

Fifty-seven accessions of Musa including cultivated clones Of 6 genomic groups (AA, AB, AAA, AAB, ABB, ABBB), M. balbisiana (BB), M. acuminata ssp. banksii (AA), M. acuminata ssp. malaccensis (AA) and M. velutina were examined for random amplified polymorphic DNA (RAPD) genetic markers using PCR with sixty 10-mer random primers. Forty-nine of 60 tested primers gave reproducible DNA amplification patterns. The number of bands resolved per amplification was primer dependent and varied from 1 to a maximum of 24. The size range of the amolification products also differed with the select& primer sequence/genotype and ranged from 0.29 to 3.0 kb. RAPD data were used to generate Jaccard's similarity coefficients which were analyzed phenetically. Phenetic analysis separated clones into distinct groupings that were in agreement with clusterings revealed when data were subsequently analyzed by principal coordinate analysis (PCO). In both the phenetic and the PCO analyses, previously unclassified cultivars grouped with cultivars previously classified for their genomic group based on morphological keys. The implications of RAPD analysis for Musa germplasm classification, clonal identification, and management are discussed.

scholarly journals Characterization of Alstroemeria Species using Random Amplified Polymorphic DNA (RAPD) Analysis

HortScience ◽  
1997 ◽  
Vol 32 (3) ◽  
pp. 482F-482 ◽  
Author(s):  
Deric D. Picton ◽  
Harrison G. Hughes
Keyword(s):  
Rapd Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Randomly Amplified Polymorphic Dna ◽  
Banding Patterns ◽  
Extraction Buffer ◽  
Rapd Pcr ◽  
High Degree ◽  
Species Hybrids ◽  
Color Variants

In this study, 11 species, hybrids, and color variants were characterized using randomly amplified polymorphic DNA (RAPD) analysis. Total genomic DNA was extracted using a 2% CTAB extraction buffer using fresh or frozen leaf material. The DNA was amplified using standard RAPD-PCR protocols utilizing 10-mer primers. All primers utilized exhibited a high degree of polymorphism in their banding patterns among the species and hybrids studied. The primers used produced ≈40 reproducible bands. It was possible to identify and uniquely distinguish all species and hybrids investigated using these bands.

Isolation and Characterization of a New Arthrospira Strain

2008 ◽  
Vol 63 (1-2) ◽  
pp. 144-150 ◽  
Author(s):  
Michele Greque de Morais ◽  
Carolina da Cruz Reichert ◽  
Francieli Dalcanton ◽  
Andrei José Durante ◽  
Luís Fernando Marins ◽  
...  
Keyword(s):  
Growth Rate ◽  
Specific Growth Rate ◽  
Specific Growth ◽  
Rapd Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Arthrospira Platensis ◽  
Central Point ◽  
Filamentous Microorganism ◽  
Isolation And Characterization

A filamentous microorganism, morphologically similar to the cyanobacterium Arthrospira, was isolated from Mangueira Lagoon in Brazil, from which Arthrospira has not previously been isolated. Random amplified polymorphic DNA (RAPD) comparison with the standard Arthrospira platensis strains LEB 52 and Paracas indicated that the organism isolated was an Arthrospira isolate, which we denominated strain LEB 18. The RAPD analysis showed conserved sequences which indicated that the three strains belonged to the same genus, and were all Arthrospira species, but there were sufficient differences between them suggesting that they were separate strains. The strain LEB 18 was cultivated in undiluted Zarrouk medium and in 60% and 20% (v/v) Zarrouk medium diluted with sterilized Mangueira Lagoon water (MLW) using illuminance rates of 32.5, 45.5 and 58.5 μmol m−2 s−1 according to a complete 32 factorial design with a triplicate central point. The strains LEB 52 and Paracas were cultived in the conditions central point. Our new isolate produced the highest specific growth rate (μmax = 0.22 d−1) in 60% Zarrouk medium diluted with MLW and illuminated with 58.5 μmol m−2 s−1 and the highest protein content (86.0% w/w).

scholarly journals Genetic Characterization ofStreptococcus iniaein Diseased Farmed Rainbow Trout (Onchorhynchus mykiss) in Iran

2012 ◽  
Vol 2012 ◽  
pp. 1-6 ◽  
Author(s):  
A. Erfanmanesh ◽  
M. Soltani ◽  
E. Pirali ◽  
S. Mohammadian ◽  
A. Taherimirghaed
Keyword(s):  
Rainbow Trout ◽  
Phylogenetic Tree ◽  
Genetic Characterization ◽  
Rapd Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Streptococcus Iniae ◽  
The North ◽  
North Part ◽  
Gram Positive Cocci

Genetic characterization of strains ofStreptococcus iniaerecovered from morbidity and mortality of farmed rainbow trout in different provinces of Iran were studied. The Gram-positive cocci isolates were obtained from the kidney tissues of diseased rainbow trout on blood agar at25°Cfor 72 h. The grown bacteria were then characterized using biochemical and molecular works. The identified 26 isolates ofS. iniaeproducing a 513 bp in PCR procedure were then compared using random amplified polymorphic DNA (RAPD) analysis using 9 random primers. The phylogenetic tree of the RAPD product using UPMGA software included these strains in one genetic group but into two clusters. The results of this study show thatS. iniaestrains from the diseased rainbow trout in the north part of Iran are genetically similar to those strains in the south and west parts of the country.

scholarly journals Characterization of the Hyphal Swelling Group of Pythium: DNA Polymorphisms and Cultural and Morphological Characteristics

Plant Disease ◽  
1998 ◽  
Vol 82 (2) ◽  
pp. 218-222 ◽  
Author(s):  
K. Kageyama ◽  
H. Uchino ◽  
M. Hyakumachi
Keyword(s):  
Rapd Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Morphological Characteristics ◽  
Its Region ◽  
Restriction Enzymes ◽  
Dna Polymorphisms ◽  
Banding Patterns ◽  
Length Polymorphisms ◽  
Polymerase Chain

The hyphal swelling (HS) group of Pythium species and P. ultimum were studied for cultural and morphological characteristics, restriction fragment length polymorphisms of the amplified internal transcribed spacer (ITS) region in nuclear rDNA, and random amplified polymorphic DNA (RAPD) analysis of genome DNA. The shape of sporangia was spherical to subspherical or lemoniform and averaged 18.1–23.0 μm. All isolates could grow at 5 to 35°C, and the rate at the optimal temperature, 30°C, was 29–34 mm/24 h. The size of the ITS region amplified by polymerase chain reaction and the banding patterns after digestion with the restriction enzymes showed no variation between the HS group and P. ultimum. No difference in banding patterns was shown between the HS group and P. ultimum by RAPD analysis with each of three primers. Isolates examined were from Japan, and results should be confirmed from other regions.

RAPD ANALYSIS OF SHRUBS BELONGING TO THE GENUS CYTISUS AND ALLIES (GENISTEAE: FABACEAE), AS AN AID FOR TAXONOMIC DISCRIMINATION

1995 ◽  
Vol 43 (4) ◽  
pp. 303-314 ◽  
Author(s):  
Fernando González-Andrés ◽  
Jesús-María Ortiz
Keyword(s):  
Molecular Markers ◽  
Numerical Analysis ◽  
Rapd Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Potential Value ◽  
High Level

Twenty-five accessions belonging to the genus Cytisus and allied taxa (Genisteae: Fabaceae) were analyzed using RAPD (Random Amplified Polymorphic DNA) techniques in order to study the usefulness of this technique for characterization of species and genera and for estimation of its potential value for further populational studies and prebreeding programs. A high level of intrapopulational variability was detected. Nevertheless, when only common bands to all the studied individuals per accession were considered for numerical analysis, the technique was useful for characterization at the specific and generic levels. The dendrogram obtained supports the generic arrangement proposed by Bisby (1981), helping to elucidate the difficult taxonomy of the studied taxa. The high intrapopulational variability indicates that RAPD could be useful for populational studies and also as molecular markers.

scholarly journals Characterization of Nomuraea rileyi strains using polymorphic DNA, virulence and enzyme activity

2003 ◽  
Vol 46 (1) ◽  
pp. 13-19 ◽  
Author(s):  
Lúcia Rosane Bertholdo Vargas ◽  
Marcelo Rossato ◽  
Rute Terezinha da Silva Ribeiro ◽  
Neiva Monteiro de Barros
Keyword(s):  
Enzyme Activity ◽  
Proteolytic Activity ◽  
Rapd Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Chitinase Activity ◽  
Nomuraea Rileyi ◽  
Control Programs ◽  
Group 2 ◽  
Integrated Pest Control

The characterization of entomopathogenic microorganisms is important for the selection of more effective strains for use in integrated pest-control programs. Five Nomuraea rileyi strains (SA86101, GU87401, SR86151, CG128 and VA9101) were characterized using random amplified polymorphic DNA (RAPD) analysis, virulence studies and assessment of chitinolytic and proteolytic activity. RAPD analysis divided the strains into two groups with a similarity coefficient of 0,76%, group 1 consisting of strains SA86101, GU87401 and SR86151 and group 2 of strains CG128 and VA9101. The LT50 varied from 165h with strain VA9101 to 246h with strain GU87401. Chitinolytic and proteolytic activity of the fungi after 144h growth in minimal medium were tested using colloidal chitin as substrate. All strains exhibited enzyme activity, with strain VA9101 having the highest chitinase activity (0,0040 mumol/mL/min the 40ºC) and strain SA86101 the highest proteolytic activity. No relationship was found between RAPD analysis, virulence and chitinase or protease activity.

scholarly journals Characterization of typical and atypical enteropathogenic Escherichia coli (EPEC) strains of the classical O55 serogroup by RAPD analysis

1999 ◽  
Vol 30 (4) ◽  
pp. 365-368 ◽  
Author(s):  
Dennys M. Girão ◽  
Sílvia Y. Bando ◽  
Valéria Brígido de C. Girão ◽  
Carlos A. Moreira-Filho ◽  
Sérgio Eduardo L. Fracalanzza ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Rapd Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Epidemiological Studies ◽  
Enteropathogenic Escherichia Coli ◽  
E Coli ◽  
Typical Epec ◽  
Rapd Method ◽  
Atypical Strains

The genetic diversity of 41 typical and atypical enteropathogenic Escherichia coli (EPEC) strains of the serogroup O55 was analyzed by using the random amplified polymorphic DNA (RAPD) method. All typical EPEC O55 strains were grouped in two clusters (A and C) and belonged to the serotype O55:H6, while cluster B included all atypical strains, which were of the serotype O55:H7. The three groups also included non-motile strains. RAPD may be a useful method for epidemiological studies on E. coli O55 infection.

scholarly journals Molecular Characterization of 28 Mango Germplasm Using RAPD

1970 ◽  
Vol 17 (1) ◽  
pp. 71-77 ◽  
Author(s):  
ML Rahman ◽  
MG Rabbani ◽  
MNA Siddique ◽  
MA Rahman ◽  
EJ Garvey ◽  
...  
Keyword(s):  
Genetic Variation ◽  
Genetic Distance ◽  
Rapd Analysis ◽  
Gene Diversity ◽  
Random Amplified Polymorphic Dna ◽  
Similarity Index ◽  
The Other ◽  
Germplasm Characterization ◽  
Upgma Dendrogram

Genetic variation and relationship among 28 mango germplasm were analyzed using Random Amplified Polymorphic DNA (RAPD). Out of 20 primers screened, four were selected, which gave 50 clear and bright fragments, out of which 48 fragments were considered polymorphic. The proportion of polymorphic loci and gene diversity values across all loci were 96% and 0.29, respectively. The UPGMA dendrogram based on genetic distance segregated the 28 mango germplasm into two main clusters. Sukul alone formed one cluster and the rest germplasm were grouped together into another cluster. Mallika and Amrapali cultivar pair was very close to each other with the highest intervarietal similarity index (87.30%) and lowest genetic distance (0.08). On the other hand, Sukul and Meghnath pair was more distant to each other with the lowest intervarietal similarity index (14.29%) and highest genetic distance (0.87). The results of the present study indicated that the RAPD analysis could be utilized by breeders for further improvement of mango varieties.Key words: Germplasm, Characterization, Mango, RAPDDOI = 10.3329/ptcb.v17i1.1123Plant Tissue Cult. & Biotech. 17(1): 71-77, 2007 (June)

Characterization of Molds from Dry-Cured Meat Products and Their Metabolites by Micellar Electrokinetic Capillary Electrophoresis and Random Amplified Polymorphic DNA PCR

2004 ◽  
Vol 67 (10) ◽  
pp. 2234-2239 ◽  
Author(s):  
A. MARTÍN ◽  
M. JURADO ◽  
M. RODRÍGUEZ ◽  
F. NÚÑEZ ◽  
J. J. CÓRDOBA
Keyword(s):  
Secondary Metabolites ◽  
Rapd Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Meat Products ◽  
Cyclopiazonic Acid ◽  
Cured Meat ◽  
Pcr Method ◽  
Electrokinetic Capillary Chromatography ◽  
Favorable Conditions

Molds are common contaminants of dry-cured meat products in which mycotoxins could be synthesized if stored under favorable conditions. Thus, efficient and accurate characterization of the toxigenic molds from dry-cured meat products is necessary. A micellar electrokinetic capillary chromatography (MECC) method was tested to analyze secondary metabolites produced by 20 mold strains commonly found in dry-cured meat products. In addition, their random amplified polymorphic DNA (RAPD) genotypes were determined by using a PCR method. Although peak profiles of the secondary metabolites differed among mold strains of different species, they were similar in the same species. MECC analysis showed that 10 of the 20 molds tested produced mycotoxins, including patulin, penicillic acid, cyclopiazonic acid, mycophenolic acid, aflatoxin B1, sterigmatocystin, and griseofulvin. The RAPD analysis yielded a different pattern for each of the mold species tested. However, strains of the same species showed similar RAPD profiles. A high correlation between RAPD analysis and MECC was observed, since strains of the same species that showed similar RAPD patterns had similar profiles of secondary metabolites. RAPD patterns with primer GO2 and MECC profiles, either singly or combined, could be of great interest to distinguish toxigenic from nontoxigenic molds in dry-cured meat products.

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