scholarly journals Genetic Characterization ofStreptococcus iniaein Diseased Farmed Rainbow Trout (Onchorhynchus mykiss) in Iran

2012 ◽  
Vol 2012 ◽  
pp. 1-6 ◽  
Author(s):  
A. Erfanmanesh ◽  
M. Soltani ◽  
E. Pirali ◽  
S. Mohammadian ◽  
A. Taherimirghaed
Keyword(s):  
Rainbow Trout ◽  
Phylogenetic Tree ◽  
Genetic Characterization ◽  
Rapd Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Streptococcus Iniae ◽  
The North ◽  
North Part ◽  
Gram Positive Cocci

Genetic characterization of strains ofStreptococcus iniaerecovered from morbidity and mortality of farmed rainbow trout in different provinces of Iran were studied. The Gram-positive cocci isolates were obtained from the kidney tissues of diseased rainbow trout on blood agar at25°Cfor 72 h. The grown bacteria were then characterized using biochemical and molecular works. The identified 26 isolates ofS. iniaeproducing a 513 bp in PCR procedure were then compared using random amplified polymorphic DNA (RAPD) analysis using 9 random primers. The phylogenetic tree of the RAPD product using UPMGA software included these strains in one genetic group but into two clusters. The results of this study show thatS. iniaestrains from the diseased rainbow trout in the north part of Iran are genetically similar to those strains in the south and west parts of the country.

scholarly journals Random Amplification of Polymorphic DNA Analysis for Genetic Characterization of Two Breeds of Dogs, German Shepherd and Japanese Spitz

2013 ◽  
Vol 13 (2) ◽  
pp. 73-78
Author(s):  
Jarina Joshsi ◽  
Lumanti Manandhar ◽  
Patima Shrestha ◽  
Rani Gupta ◽  
Rojlina Manadhar ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Rapd Markers ◽  
Dna Analysis ◽  
Genetic Characterization ◽  
Random Amplified Polymorphic Dna ◽  
Neighbor Joining ◽  
German Shepherd ◽  
Polymorphic Loci ◽  
Random Amplification

Random amplified polymorphic DNA (RAPD) markers were used to study genetic diversity in dog samples belonging to populations of German Shepherd and Japanese Spitz. A total of twelve samples were typed using eight RAPD primers. Out of eight primers, three primers gave result in six individuals of dogs. The phylogenetic tree constructed by the neighbor joining method based on Nei. Original measures revealed highest genetic identity found in German Shepherd as 0.9444 and highest genetic distance as 1.2809. The analysis predicts the number of polymorphic loci as 15 and the percentage of polymorphic loci as 83.3. Nepal Journal of Science and Technology Vol. 13, No. 2 (2012) 73-78 DOI: http://dx.doi.org/10.3126/njst.v13i2.7717

Ecological, morphological and genetic characterization of sympatric Haemonchus spp. parasites of domestic ruminants in Mauritania

Parasitology ◽  
1995 ◽  
Vol 110 (4) ◽  
pp. 483-492 ◽  
Author(s):  
P. Jacquiet ◽  
J. F. Humbert ◽  
A. M. Comes ◽  
J. Cabaret ◽  
A. Thiam ◽  
...  
Keyword(s):  
Rainy Season ◽  
Genetic Characterization ◽  
Random Amplified Polymorphic Dna ◽  
Intraspecific Variability ◽  
Species Traits ◽  
The Other ◽  
Survival Strategies ◽  
Zebu Cattle ◽  
Sheep And Goats

SUMMARYThe 4 species of ruminants (dromedary, zebu cattle, sheep and goat) in arid areas of Mauritania harboured Haemonchus spp. as the most frequent internal parasite. This was a rare situation where the 3 putative species, H. longistipes (dromedary), H. placet (zebu cattle) and H. contortus (sheep and goat) occurred sympatrically. The study was undertaken on hosts slaughtered at the Nouakchott abattoir, on the basis of monthly collection of worms. The environment was very unfavourable to H. placei and unfavourable to H. contortus, as intensity of infection remained low throughout the year, whereas infection in the dromedary was 10 to 20-fold higher. The survival strategies during the long, dry season were different: the surviving stages were either 4th-stage larvae in digesta (dromedaries), 4th-stage larvae either in digesta or mucosae (cattle), or 4th-stage larvae in mucosae and few adults (sheep and goats). The prolificacy of female worms, indicative of the potential to contaminate pastures, was similar for all Haemonchus spp. in the rainy season. H. longistipes behave differently during the pre-rainy season as no increase of prolificacy could be demonstrated as observed in the other species. Traits of vulvar morphology are considered as markers of ecological adaptation and were studied. The knobbed and smooth female morphs (in equal proportions) were the most frequent in H. longistipes, the knobbed morph out-numbered the other morphs in H. placei, and all 3 morphs were present in sheep and goats with the linguiform form being predominant. Genetic characterization of the 3 species was performed by means of Random Amplified Polymorphic DNA (RAPD). Three groups were obtained from analysis of these data: 1 group with individuals of H. contortus, 1 group with individuals of H. placei, and 1 group with individuals of H. longistipes. This indicated that, although the 3 species were valid, H. contortus and H. placei were more similar. Intraspecific variability was 2-fold higher in H. contortus than in the 2 other species. The ecological, morphological and genetical studies showed that H. longistipes, H. placei and H. contortus could be arranged in increasing order of variability.

Characterization of metals in air and soil near a steel making plant in the North part of Italy

2016 ◽  
Vol 27 (4) ◽  
pp. 441-451 ◽  
Author(s):  
Elena C. Rada ◽  
Marco Ragazzi ◽  
Marco Tubino ◽  
Andrea Gambaro ◽  
Clara Turetta ◽  
...  
Keyword(s):  
Decision Makers ◽  
Content Type ◽  
Best Available Technologies ◽  
The North ◽  
Steel Making ◽  
North Part ◽  
Negative Role ◽  
The Impact

Purpose – The purpose of this paper is to define suitable tracers that are particularly significant in assessing the impact on the surrounding environment caused by a steel making plant. Design/methodology/approach – The authors focussed on the detection and quantification of inorganic pollutants such as heavy metals in the soil and in the air in the surrounding area of the steel making plant. The presented data, concerning a plant in the North part of Italy, come from an approach that took into account the advantages of different devices and sampling criteria: apart from conventional devices, two kinds of deposimeters have been used (for characterizing either total or wet/dry depositions). Their locations have been chosen taking into account the distance from the plant. Findings – The paper demonstrates that the presence of diffused emissions plays an important (negative) role still today; thus a modern plant should reduce this kind of emission in order to be accepted from the population living in the surroundings. In the analyzed case study, the benefits of adopting the best available technologies can be seen from the temporal variability of the incidence of the plant. Originality/value – The value of the paper is in the support that it can give to decision makers that must manage a steel making plant in an area.

scholarly journals Genetic characterization of the Moxotó goat breed using RAPD markers

2005 ◽  
Vol 40 (3) ◽  
pp. 233-239 ◽  
Author(s):  
Rejane Rodrigues de Oliveira ◽  
Andréa Alves do Egito ◽  
Maria Norma Ribeiro ◽  
Samuel Rezende Paiva ◽  
Maria do Socorro Maués Albuquerque ◽  
...  
Keyword(s):  
Genetic Variability ◽  
Rapd Markers ◽  
Genetic Characterization ◽  
Random Amplified Polymorphic Dna ◽  
Genetic Distances ◽  
Goat Breed ◽  
Breeding Programs ◽  
Analysis Of Molecular Variance ◽  
Polymorphic Bands

The objective of this study was to verify the genetic diversity between and within seven populations of Moxotó goat (n = 264) from the States of Pernambuco, Paraíba and Rio Grande do Norte, using RAPD (Random Amplified Polymorphic DNA). Moxotó, as well as other naturalized breeds, suffers genetic losses due to the indiscriminate miscegenation with breeds raised in the Northeast Region of Brazil. The genetic characterization of these genetic resources is essential to conservation and breeding programs. DNA was extracted from lymphocytes using a non-organic protocol. The 16 primers used were selected from 120 decamer oligonucleotide primers and generated 56 polymorphic bands. The analysis of molecular variance (AMOVA) showed that the greater part of total genetic variability (71.55%) was due to differences between individuals within populations, while 21.21% was among populations. The analysis of variance among the pairs of populations demonstrated that the populations located in Floresta, PE x Angicos, RN presented a smaller value of intrapopulational differentiation (8.9%), indicating low genetic variability among them. Nei's genetic distances varied between 0.0546 and 0.1868 in the populations. The dendrogram generated showed that the Canindé breed, used as outgroup, clustered with the populations of Moxotó, indicating a possible common origin of the naturalized goat breeds.

scholarly journals 678 PB 186 RAPD GENETIC MARKERS FOR CHARACTERIZATION OF MUSA GENETIC RESOURCES

HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 530a-530
Author(s):  
R.L. Jarret ◽  
K.V. Bhat
Keyword(s):  
Genetic Markers ◽  
Size Range ◽  
Rapd Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Dna Amplification ◽  
Principal Coordinate Analysis ◽  
Phenetic Analysis ◽  
Similarity Coefficients ◽  
Primer Sequence

Fifty-seven accessions of Musa including cultivated clones Of 6 genomic groups (AA, AB, AAA, AAB, ABB, ABBB), M. balbisiana (BB), M. acuminata ssp. banksii (AA), M. acuminata ssp. malaccensis (AA) and M. velutina were examined for random amplified polymorphic DNA (RAPD) genetic markers using PCR with sixty 10-mer random primers. Forty-nine of 60 tested primers gave reproducible DNA amplification patterns. The number of bands resolved per amplification was primer dependent and varied from 1 to a maximum of 24. The size range of the amolification products also differed with the select& primer sequence/genotype and ranged from 0.29 to 3.0 kb. RAPD data were used to generate Jaccard's similarity coefficients which were analyzed phenetically. Phenetic analysis separated clones into distinct groupings that were in agreement with clusterings revealed when data were subsequently analyzed by principal coordinate analysis (PCO). In both the phenetic and the PCO analyses, previously unclassified cultivars grouped with cultivars previously classified for their genomic group based on morphological keys. The implications of RAPD analysis for Musa germplasm classification, clonal identification, and management are discussed.

scholarly journals Characterization of Alstroemeria Species using Random Amplified Polymorphic DNA (RAPD) Analysis

HortScience ◽  
1997 ◽  
Vol 32 (3) ◽  
pp. 482F-482 ◽  
Author(s):  
Deric D. Picton ◽  
Harrison G. Hughes
Keyword(s):  
Rapd Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Randomly Amplified Polymorphic Dna ◽  
Banding Patterns ◽  
Extraction Buffer ◽  
Rapd Pcr ◽  
High Degree ◽  
Species Hybrids ◽  
Color Variants

In this study, 11 species, hybrids, and color variants were characterized using randomly amplified polymorphic DNA (RAPD) analysis. Total genomic DNA was extracted using a 2% CTAB extraction buffer using fresh or frozen leaf material. The DNA was amplified using standard RAPD-PCR protocols utilizing 10-mer primers. All primers utilized exhibited a high degree of polymorphism in their banding patterns among the species and hybrids studied. The primers used produced ≈40 reproducible bands. It was possible to identify and uniquely distinguish all species and hybrids investigated using these bands.

scholarly journals Genetic characterization of Xylella fastidiosa isolated from citrus and coffee plants

2007 ◽  
Vol 64 (5) ◽  
pp. 482-485 ◽  
Author(s):  
Vicente Savonitti Miranda ◽  
Paulo Roberto Silva Farias ◽  
Sérgio Rufo Roberto ◽  
Pedro Magalhães Lacava
Keyword(s):  
Genetic Characterization ◽  
Xylella Fastidiosa ◽  
Random Amplified Polymorphic Dna ◽  
Negative Bacterium ◽  
Similarity Coefficients ◽  
Gram Negative ◽  
Coffee Plants ◽  
The Many ◽  
Group D

The Citrus Variegated Chlorosis and the Coffee Leaf Scorch are some of the many destructive diseases caused by Xylella fastidiosa, a gram-negative bacterium limited to the xylem of affected plants. As its genetic characterization is still not well established, different isolates of X. fastidiosa from citrus and coffee were evaluated through RAPD (Random Amplified Polymorphic DNA) technique to characterize and classify these isolates based on similarity coefficients. Sixteen isolates of X. fastidiosa were used on this trial, obtained from citrus, coffee and almond. The genetic polymorphism evaluation was performed using six arbitrary 10-base primer pairs. It was possible to establish a dendogram in which the isolates were classified into five groups (A, B, C, D and E). A prevalence of citrus isolates in groups A and D was observed. In groups B and C, there was a prevalence of coffee isolates meanwhile the group D consisted of the almond isolate, solely.

Isolation and Characterization of a New Arthrospira Strain

2008 ◽  
Vol 63 (1-2) ◽  
pp. 144-150 ◽  
Author(s):  
Michele Greque de Morais ◽  
Carolina da Cruz Reichert ◽  
Francieli Dalcanton ◽  
Andrei José Durante ◽  
Luís Fernando Marins ◽  
...  
Keyword(s):  
Growth Rate ◽  
Specific Growth Rate ◽  
Specific Growth ◽  
Rapd Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Arthrospira Platensis ◽  
Central Point ◽  
Filamentous Microorganism ◽  
Isolation And Characterization

A filamentous microorganism, morphologically similar to the cyanobacterium Arthrospira, was isolated from Mangueira Lagoon in Brazil, from which Arthrospira has not previously been isolated. Random amplified polymorphic DNA (RAPD) comparison with the standard Arthrospira platensis strains LEB 52 and Paracas indicated that the organism isolated was an Arthrospira isolate, which we denominated strain LEB 18. The RAPD analysis showed conserved sequences which indicated that the three strains belonged to the same genus, and were all Arthrospira species, but there were sufficient differences between them suggesting that they were separate strains. The strain LEB 18 was cultivated in undiluted Zarrouk medium and in 60% and 20% (v/v) Zarrouk medium diluted with sterilized Mangueira Lagoon water (MLW) using illuminance rates of 32.5, 45.5 and 58.5 μmol m−2 s−1 according to a complete 32 factorial design with a triplicate central point. The strains LEB 52 and Paracas were cultived in the conditions central point. Our new isolate produced the highest specific growth rate (μmax = 0.22 d−1) in 60% Zarrouk medium diluted with MLW and illuminated with 58.5 μmol m−2 s−1 and the highest protein content (86.0% w/w).

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