scholarly journals Reused Plant Fried Oil: A Case Study with Home-Made Soaps

Processes ◽  
2021 ◽  
Vol 9 (3) ◽  
pp. 529
Author(s):  
Bojan Antonic ◽  
Dani Dordevic ◽  
Simona Jancikova ◽  
Bohuslava Tremlova ◽  
Marcela Nejezchlebova ◽  
...  
Keyword(s):  
Methicillin Resistant Staphylococcus Aureus ◽  
Gram Negative Bacteria ◽  
Polar Material ◽  
Waste Frying Oil ◽  
Gram Positive Bacteria ◽  
Soap Production ◽  
Oil Utilization ◽  
Total Polar Material ◽  
Fried Oil

The study aimed to analyze the possibility of waste frying oil utilization in home-made soap production. Soaps were made from unheated and fried rapeseed, sunflower and palm oils that had total polar material (TPM) values up to 24%. Physicochemical and microbial analyses were performed on produced samples to check their quality. The hardness increased with the degradation level of rapeseed and palm oils, and opposite findings were obtained for sunflower-made soaps. The highest malondialdehyde (MDA) contents were recorded for sunflower oil-made samples, with the maximum of 6.61 µg/g, and the lowest for the palm oil-made samples, with the maximum of 0.94 µg/g. The antimicrobial assessment showed no significant (p > 0.05) differences between control soap samples and soaps made of oils with the highest TPM value. Gram-positive bacteria (methicillin-resistant Staphylococcus aureus: MRSA) were the most sensitive chosen microorganisms, compared to Gram-negative bacteria and yeasts. The obtained results did not show exact differences between experimentally produced soap samples from fried or not fried oils; these findings highlight the potential of home-made soap production from this byproduct.

Toxicity of hydrogen peroxide produced by electroplated coatings to pathogenic bacteria

1998 ◽  
Vol 44 (5) ◽  
pp. 441-447 ◽  
Author(s):  
Zhi-Hong Zhao ◽  
Yoshikazu Sakagami ◽  
Tetsuya Osaka
Keyword(s):  
Hydrogen Peroxide ◽  
Metal Ions ◽  
Pathogenic Bacteria ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Survival Rates ◽  
Gram Negative Bacteria ◽  
Bacterial Survival ◽  
Gram Positive Bacteria ◽  
Inhibitory Ability ◽  
Nickel Zinc

The ability of various electroplated coatings (cobalt, zinc, copper, and cobalt-containing alloys of nickel, zinc, chromium, etc.) to inhibit the growth of pathogenic bacteria (Gram-positive bacteria Enterococcus faecalis and methicillin-resistant Staphylococcus aureus and Gram-negative bacteria Escherichia coli, Pseudomonas aeruginosa, andKlebsiella pneumoniae) was determined by a drop-method antibacterial experiment. The amounts of H2O2 produced and metal ions dissolved from the surfaces of various electroplated coatings were measured and it was found that the inhibitory ability of coatings corresponded to the amounts of H2O2 produced. The more significant the inhibition of the coating to bacterial growth, the greater the amount of H2O2 production. In addition, the bacterial survival rates on the surfaces of coatings were almost zero when H2O2 was produced in amounts greater than 10-6 mmol/cm2. However, the dominant concentrations of metal ions dissolved from coatings were outside of the bacterial lethal range.Key words: electroplated coatings, pathogenic bacteria, inhibitory ability, hydrogen peroxide.

scholarly journals Synthesis, Characterization and Antimicrobial Activity Studies of Chlorocobaloximes with Neutral Bases Containing Amine Functionality

2021 ◽  
Vol 33 (11) ◽  
pp. 2781-2788
Author(s):  
V. Vijaikanth ◽  
S. Vasuki ◽  
C. Immanuel David ◽  
L. Chandran ◽  
S. Sowmya ◽  
...  
Keyword(s):  
Methicillin Resistant Staphylococcus Aureus ◽  
Spectroscopic Techniques ◽  
Gram Negative Bacteria ◽  
Zone Of Inhibition ◽  
Gram Positive ◽  
Gram Negative ◽  
Gram Positive Bacteria ◽  
Dimethyl Glyoxime ◽  
Uv Visible ◽  
Ethyl Amine

The inorganic cobaloximes of type [Co(Cl)(dmgH)2B]; where dmgH = dimethyl glyoxime and B = neutral bases: glycine, ethyl amine, 2-aminopyridine, 4-aminopyridine, 2,6-diaminopyridine, aniline and 1-napthylamine have been synthesized. The synthesized cobaloximes were characterized by IR, UV-visible, 1H NMR and 13C NMR spectroscopic techniques. The cobaloxime complexes were screened for their antibacterial activity against methicillin resistant Staphylococcus aureus (MRSA) and Acinetobacter baumannii by the zone of inhibition test, biofilm eradication on biomaterial using catheter and modified Congo red agar method. It has been found that the cobaloxime complexes exhibit inhibition against both gram positive and gram negative bacteria and the cobaloximes showed better inhibition towards Gram-negative bacteria compared to Gram-positive bacteria.

Analysis of Total Polar Material in Selected Indian Snack’s Fried Oil

2021 ◽  
Vol 13 (2) ◽  
pp. 561-570
Author(s):  
R. Kumar ◽  
B. Bhattacharya ◽  
T. Agarwal ◽  
S. Chakkaravarthi
Keyword(s):  
Partial Least Square ◽  
Frying Oil ◽  
Least Square ◽  
Partial Least Square Regression ◽  
Good Prediction ◽  
Polar Material ◽  
Total Polar Material ◽  
Prediction Capability ◽  
Fried Oil

The study was envisaged to examine the quality of frying oil used by street food vendors for two of the most popular food items viz. Samosa and Jalebi in India. Changes in the quality of frying oil were analysed by analysing the total polar material (TPM) content in the oil using an oil tester and Attenuated total reflectance–Fourier transform infrared (ATR-FTIR) spectroscopy. Total 143 oil samples were collected at different frying times, i.e. 0, 2 and 4 h from five different Samosa and Jalebi vendors. In both the fried food oil samples, TPM content increased with increasing frying time. The TPM content in the 4 h fried oil samples of Jalebi was significantly (p< 0.001) higher than the samosa fried oil. Partial Least Square Regression (PLS) model based on the 1st derivative FTIR spectra exhibited good prediction capability for TPM values with a high regression coefficient (R2 ≥ 0.99) and low root mean square error (RMSE).

scholarly journals Actinomycetes isolated from soils collected in adjacent areas of Hochiminh city: sources of potential antibiotics

2015 ◽  
Vol 18 (2) ◽  
pp. 75-84
Author(s):  
Huyen Thi Pham ◽  
Thu Huynh ◽  
Phuong Ngoc Uyen Pham ◽  
Tao Thi Ly Vo
Keyword(s):  
Staphylococcus Aureus ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Soil Samples ◽  
Viet Nam ◽  
Gram Negative Bacteria ◽  
Gram Positive ◽  
Gram Positive Bacteria ◽  
Sequencing Method ◽  
Rdna Sequencing ◽  
Resistant Form

Actinomycetes are Gram-positive bacteria resembling filamentous fungi in shape. The majority of natural antibiotics useful today are obtained from actinomycetes. This study isolated actinomycetes from soil samples collected in adjacent areas of HoChiMinh City, Việt Nam. One of the isolates was Streptomyces flaveus determined using 16S rDNA sequencing method. S. flaveus was capable of inhibiting the growth of both Gram-positive and Gram-negative bacteria, especially the Staphylococcus aureus and its multiantibiotic resistant form – the methicillin resistant Staphylococcus aureus (MRSA). MRSA is at present a serious threat to human health not only in Vietnam but also in many other countries around the world. However, antibiotics indicated for the treatment of diseases caused by the MRSA are currently not much effective. Thus, S. flaveus in this study will be a source of potential antibiotics against diseases caused by the MRSA.

Rapid demonstration of septic or infectious arthritis due to Gram-negative bacteria

1992 ◽  
Vol 50 (1) ◽  
pp. 686-687
Author(s):  
Jacob S. Hanker ◽  
Paul R. Gross ◽  
Beverly L. Giammara
Keyword(s):  
Chronic Arthritis ◽  
Blood Cultures ◽  
Gram Negative Bacteria ◽  
Infectious Arthritis ◽  
Bacterial Arthritis ◽  
Gram Positive ◽  
Gram Negative ◽  
Gram Positive Bacteria

Blood cultures are positive in approximately only 50 per cent of the patients with nongonococcal bacterial infectious arthritis and about 20 per cent of those with gonococcal arthritis. But the concept that gram-negative bacteria could be involved even in chronic arthritis is well-supported. Gram stains are more definitive in staphylococcal arthritis caused by gram-positive bacteria than in bacterial arthritis due to gram-negative bacteria. In the latter situation where gram-negative bacilli are the problem, Gram stains are helpful for 50% of the patients; they are only helpful for 25% of the patients, however, where gram-negative gonococci are the problem. In arthritis due to gram-positive Staphylococci. Gramstained smears are positive for 75% of the patients.

Antibacterial activity of magnesium oxide nanoparticles prepared by Calcination method

2019 ◽  
Vol 10 (03) ◽  
Author(s):  
Elaf Ayad Kadhem ◽  
Miaad Hamzah Zghair ◽  
Sarah , Hussam H. Tizkam, Shoeb Alahmad Salih Mahdi ◽  
Hussam H. Tizkam ◽  
Shoeb Alahmad
Keyword(s):  
Antibacterial Activity ◽  
Magnesium Oxide ◽  
Diffusion Method ◽  
Oxide Nanoparticles ◽  
Gram Negative Bacteria ◽  
Gram Positive ◽  
Gram Negative ◽  
Gram Positive Bacteria ◽  
Magnesium Oxide Nanoparticles ◽  
Agar Disc

magnesium oxide nanoparticles (MgO NPs) were prepared by simple wet chemical method using different calcination temperatures. The prepared NPs were characterized by Electrostatic Discharge (ESD), Scanning Electron Microscope (SEM) and X-ray Diffraction (XRD). It demonstrates sharp intensive peak with the increase of crystallinty and increase of the size with varying morphologies with respect to increase of calcination temperature. Antibacterial studies were done on gram negative bacteria (E.coli) and gram positive bacteria (S.aureus) by agar disc diffusion method. The zones of inhibitions were found larger for gram positive bacteria than gram negative bacteria, this mean, antibacterial MgO NPs activity more active on gram positive bacteria than gram negative bacteria because of the structural differences. It was found that antibacterial activity of MgO NPs was found it has directly proportional with their concentration.

Synthesis, Spectral and Antimicrobial Investigation of 2-(Naphthalenel-ylamino)-2- Phenylacetonitrile and 1, 10-Phenanthroline with Five Divalent Transition Metal Ions

2019 ◽  
Vol 10 (01) ◽  
Author(s):  
Mohammed Al-Amery1 ◽  
Ashraf Saad Rasheed ◽  
Dina A. Najeeb
Keyword(s):  
Transition Metal ◽  
Metal Ions ◽  
Transition Metal Ions ◽  
Molar Conductance ◽  
Octahedral Geometry ◽  
Mixed Ligand ◽  
Gram Negative Bacteria ◽  
Magnetic Studies ◽  
Gram Positive Bacteria

Five new mixed ligand metal complexes have been synthesized by the reaction of divalent transition metal ions (Hg, Ni, Zn, Cu and Cd) with 2-(naphthalen-l-ylamino)-2-phenylacetonitrile (L1 ) and 1,10-phenanthroline (L2). The coordination likelihood of the two ligands toward metal ions has been suggested in the light of elemental analysis, UV-Vis spectra, FTIR, 1H-NMR, flam atomic absorption, molar conductance and magnetic studies. Results data suggest that the octahedral geometry for all the prepared complexes. Antibacterial examination of synthesized complexes in vitro was performed against four bacterias. Firstly, Gram-negative bacteria namely, Pseudomonas aerugin and Escherichia. Secondly, Gram-positive bacteria namely, Bacillus subtilis, Staphylococcuaurouss. Results data exhibit that the synthesized complexes exhibited more biological activity than tetracycline pharmaceutical.

BAKTERI PADA KARANG SCLERACTINIA DI KAWASAN PERAIRAN BUNAKEN, MOROTAI DAN RAJA AMPAT

2020 ◽  
Vol 8 (1) ◽  
pp. 122
Author(s):  
Eghbert Eghbert Elvan Eghbert Elvan Ampou ◽  
Iis Iis Triyulianti ◽  
Nuryani Widagti ◽  
Suciadi Catur Nugroho ◽  
Yuli Pancawati
Keyword(s):  
Scleractinian Coral ◽  
Gram Negative Bacteria ◽  
Hard Coral ◽  
Field Sampling ◽  
Bacterial Isolation ◽  
Gram Positive ◽  
Coral Mucus ◽  
Gram Negative ◽  
Gram Positive Bacteria

Research on hard coral (Scleractinian coral) contaminated with bacteria is still not much done, especially in Indonesian waters. This study took samples of coral mucus in 2010 at 3 (three) different locations, namely Bunaken (May); Morotai (September) and Raja Ampat (November), which focused on the analysis of Research on hard coral (Scleractinian coral) contaminated with bacteria is still not much done, especially in Indonesian waters. This study took samples of coral mucus in 2010 at 3 (three) different locations, namely Bunaken (May); Morotai (September) and Raja Ampat (November), which focused on the analysis of gram-positive and gram-negative bacteria. The method used for field sampling is time swim, which is by diving at a depth of 5-10 meters for ± 30 minutes and randomly taking samples of coral mucus using siring or by taking directly on corals (reef branching). Mucus samples were analyzed by bacterial isolation in the laboratory. The result shows that there were differences between gram-positive and gram-negative bacteria in the three research sites and that gram-positive bacteria were higher or dominant. Further research that can identify the bacteria species and explain its relationship to the ecosystem is highly recommended.Keywords: Bacteria, Scleractinian coral, gram-positive and -negative, Bunaken, Morotai, Raja Ampat  AbstrakPenelitian tentang karang keras (Scleractinian coral) yang terkontaminasi bakteri masih belum banyak dilakukan, terutama di perairan Indonesia. Penelitian ini mengambil sampel mucus karang pada tahun 2010 di 3 (tiga) lokasi berbeda, yakni Bunaken (Mei); Morotai (September) dan Raja Ampat (November), yang difokuskan pada analisis bakteri gram postif dan gram negatif. Metode yang digunakan untuk pengambilan sampel di lapangan adalah time swim, yaitu dengan penyelaman pada kedalaman 5-10 meter selama ±30 menit dan mengambil sampel mucus karang secara acak menggunakan siring atau dengan mengambil langsung pada karang (fraksi cabang). Sampel mucus dianalisis dengan cara isolasi bakteri di laboratorium. Hasil analisis menunjukkan bahwa ada perbedaan antara bakteri gram positif dan gram negative di tiga lokasi survei dan bakteri gram positif lebih tinggi atau dominan. Penelitian lebih lanjut yang dapat menentukan jenis bakteri serta menjelaskan hubungannya dengan ekosistem sangat disarankan untuk dilakukan.Kata Kunci : Bakteri, Scleractinian coral, gram positif dan negatif, Bunaken, Morotai, Raja Ampat

A Review of the Characteristics, Properties and Methods for Quantification of Roxithromycin

2020 ◽  
Vol 16 ◽  
Author(s):  
Marcus Vinicius Martins Rubatino ◽  
Ana Laura Araújo Santos ◽  
Rayssa Araújo dos Santos ◽  
Magali Benjamim de Araújo
Keyword(s):  
Analytical Methods ◽  
Environmental Samples ◽  
Biological Fluids ◽  
Pharmaceutical Preparations ◽  
Gram Negative Bacteria ◽  
Analytical Technique ◽  
Detection Systems ◽  
Gram Positive Bacteria ◽  
Microbiological Characteristics ◽  
Biological And Environmental Samples

: Roxithromycin is one of the most frequently used macrolide antibiotics, a safe group of antimicrobials that acts against Gram-positive bacteria and some Gram-negative bacteria. It is sold in several countries in different dosage forms (tablets and capsules) and strengths (50, 100, 150 and 300 mg). Several analytical methods have been described to quantify roxithromycin in different matrices, such as biological and environmental samples and food. Identifying the main characteristics of the drugs and selecting appropriate analytical methods for their quantification are of paramount importance for understanding the behavior of drugs, metabolites and impurities. This review presents the physicalchemical and microbiological characteristics, properties as well as methods for quantification of roxithromycin in biological fluids, pharmaceutical preparations, food and environmental samples. HPLC coupled to various detection systems is the most used analytical technique to determine roxithromycin in these matrices. Although many analytical methods have been reported for the analysis of this drug, it is very important to include in this context a prospective view, in order to implement new analytical technologies for the quality control of this antimicrobial that contribute to the preservation of economic and environmental impacts.

scholarly journals N-Doped Carbon Quantum Dots as Fluorescent Bioimaging Agents

Crystals ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 789
Author(s):  
Shih-Fu Ou ◽  
Ya-Yun Zheng ◽  
Sin-Jen Lee ◽  
Shyi-Tien Chen ◽  
Chien-Hui Wu ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Carbon Nanomaterials ◽  
Graphene Quantum Dots ◽  
Carbon Quantum Dots ◽  
Gram Negative Bacteria ◽  
Gram Positive ◽  
Gram Negative ◽  
Gram Positive Bacteria ◽  
Fluorescence Characteristics ◽  
And Staphylococcus Aureus

Graphene quantum dots, carbon nanomaterials with excellent fluorescence characteristics, are advantageous for use in biological systems owing to their small size, non-toxicity, and biocompatibility. We used the hydrothermal method to prepare functional N-doped carbon quantum dots (N-CQDs) from 1,3,6-trinitropyrene and analyzed their ability to fluorescently stain various bacteria. Our results showed that N-CQDs stain the cell septa and membrane of the Gram-negative bacteria Escherichia coli, Salmonellaenteritidis, and Vibrio parahaemolyticus and the Gram-positive bacteria Bacillus subtilis, Listeria monocytogenes, and Staphylococcus aureus. The optimal concentration of N-CQDs was approximately 500 ppm for Gram-negative bacteria and 1000 ppm for Gram-positive bacteria, and the exposure times varied with bacteria. N-Doped carbon quantum dots have better light stability and higher photobleaching resistance than the commercially available FM4-64. When excited at two different wavelengths, N-CQDs can emit light of both red and green wavelengths, making them ideal for bioimaging. They can also specifically stain Gram-positive and Gram-negative bacterial cell membranes. We developed an inexpensive, relatively easy, and bio-friendly method to synthesize an N-CQD composite. Additionally, they can serve as a universal bacterial membrane-staining dye, with better photobleaching resistance than commercial dyes.

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