scholarly journals Actinomycetes isolated from soils collected in adjacent areas of Hochiminh city: sources of potential antibiotics

2015 ◽  
Vol 18 (2) ◽  
pp. 75-84
Author(s):  
Huyen Thi Pham ◽  
Thu Huynh ◽  
Phuong Ngoc Uyen Pham ◽  
Tao Thi Ly Vo
Keyword(s):  
Staphylococcus Aureus ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Soil Samples ◽  
Viet Nam ◽  
Gram Negative Bacteria ◽  
Gram Positive ◽  
Gram Positive Bacteria ◽  
Sequencing Method ◽  
Rdna Sequencing ◽  
Resistant Form

Actinomycetes are Gram-positive bacteria resembling filamentous fungi in shape. The majority of natural antibiotics useful today are obtained from actinomycetes. This study isolated actinomycetes from soil samples collected in adjacent areas of HoChiMinh City, Việt Nam. One of the isolates was Streptomyces flaveus determined using 16S rDNA sequencing method. S. flaveus was capable of inhibiting the growth of both Gram-positive and Gram-negative bacteria, especially the Staphylococcus aureus and its multiantibiotic resistant form – the methicillin resistant Staphylococcus aureus (MRSA). MRSA is at present a serious threat to human health not only in Vietnam but also in many other countries around the world. However, antibiotics indicated for the treatment of diseases caused by the MRSA are currently not much effective. Thus, S. flaveus in this study will be a source of potential antibiotics against diseases caused by the MRSA.

scholarly journals Antimicrobial Activity of Gardenia jasminoides Callus and Crude Leaf Extracts for some Organic Solvents against some Pathogenic Bacteria and Yeasts

2014 ◽  
Vol 8 (3) ◽  
pp. 40-45
Author(s):  
Zina Hashem Shehab ◽  
Huda Suhail Abid ◽  
Sumaya Fadhil Hamad ◽  
Sara Haitham
Keyword(s):  
Staphylococcus Aureus ◽  
Candida Albicans ◽  
Pathogenic Bacteria ◽  
Saccharomyces Boulardii ◽  
Gram Negative Bacteria ◽  
Proteus Vulgaris ◽  
Gram Positive ◽  
Gram Negative ◽  
Gram Positive Bacteria ◽  
Gardenia Jasminoides

The study was conducted to evaluate the inhibitory activity of methanol extract of Gardenia jasminoides leaves compared with leaf crude extracts for some organic solvents namely Methanol, Ethanol, Petroleum ether, Asetone and Chloroform on growth of some pathogenic bacteria and yeast, which included four gram positive isolates Staphylococcus aureus, Enterococcus faecalis, Streptococcus pyogenes and Bacillus cereus and gram negative isolates Escherichia coli, Salmonella typhi, Proteus vulgaris and Pseudomonas aeruginosa and some yeasts Candida albicans and Saccharomyces boulardii, by using well diffusion method. The inhibitory activity of extracts in the tested bacterial strains and yeasts was varied according to the type of extracting solvents and are tested microorganisms. The methanol callus extract which grown on Murashige and Skoog (MS) media by using (Naphthalen acitic acid) NAA and (Benzyle adenine) BA as growth regulator highly effective as compared to the other extracts as for inhibition of three gram positive bacteria and three gram negative bacteria,which include Staphylococcus aureus and, Proteus vulgaris, followed by acetone and ethanolic extracts which include two gram positive bacteria and two gram negative bacteria. All extracts had highly effect in growth of Candida albicans while all crude extracts didn’t show any sensitivity against Saccharomyces boulardii, and when we’d done (High Performance Liquid Chromatography) HPLC test for detection of some active compound we found Quinic acid, Iridiods glycosides and Crocin which its rate in fresh callus was higher than fresh leaves.

scholarly journals Chemical Composition and Antibacterial Activity of the Essential Oil of Espeletia nana

2012 ◽  
Vol 7 (5) ◽  
pp. 1934578X1200700 ◽  
Author(s):  
Alexis Peña ◽  
Luis Rojas ◽  
Rosa Aparicio ◽  
Libia Alarcón ◽  
José Gregorio Baptista ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Diffusion Method ◽  
Gram Negative Bacteria ◽  
Gram Positive ◽  
Disk Diffusion Method ◽  
Gram Negative ◽  
Gram Positive Bacteria

The essential oil of the leaves of Espeletia nana Cuatrec, obtained by hydrodistillation, was analyzed by GC-MS, which allowed the identification of 24 components, which made up 99.9% of the oil. The most abundant compounds were α-pinene (38.1%), β-pinene (17.2%), myrcene (15.0%), spathulenol (4.2%), bicyclogermacrene (4.0%), α-zingiberene (4.0%), and γhimachalene (3.7%). Antibacterial activity was tested against Gram-positive and Gram-negative bacteria using the agar disk diffusion method. Activity was observed only against Gram-positive bacteria. MIC values were determined for Staphylococcus aureus ATCC 25923(200 μg/mL) and Enterococcus faecalis ATCC 29212 (600 μg/mL).

Survival of some non-starter bacteria in naturally ripened and enzyme-accelerated Cheddar cheese

1988 ◽  
Vol 55 (4) ◽  
pp. 597-602 ◽  
Author(s):  
Lydia Bautista ◽  
Rohan G. Kroll
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Enterococcus Faecalis ◽  
Enzyme System ◽  
Cheddar Cheese ◽  
Gram Negative Bacteria ◽  
Gram Positive ◽  
Gram Negative ◽  
Ripening Period ◽  
Gram Positive Bacteria

SummaryEffects of the addition of a proteinase (Neutrase 1–5S) and a peptidase (aminopeptidase DP-102) as agents for accelerating the ripening of Cheddar cheese on the survival of some non-starter bacteria (Staphylococcus aureus, Enterococcus faecalis, Escherichia coliand aSalmonellasp.) were studied throughout a 4-month ripening period. The enzymes were found to have no significant effect on the survival of the Gram-positive bacteria but some significant effects were observed, at some stages of the ripening period, with the Gram-negative bacteria in that lower levels were recovered from cheeses treated with the enzyme system.

scholarly journals Bactericidal effects of non-thermal argon plasma in vitro, in biofilms and in the animal model of infected wounds

2011 ◽  
Vol 60 (1) ◽  
pp. 75-83 ◽  
Author(s):  
Svetlana A. Ermolaeva ◽  
Alexander F. Varfolomeev ◽  
Marina Yu. Chernukha ◽  
Dmitry S. Yurov ◽  
Mikhail M. Vasiliev ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Plasma Treatment ◽  
Pathogenic Bacteria ◽  
Argon Plasma ◽  
Burkholderia Cenocepacia ◽  
Gram Negative Bacteria ◽  
Gram Positive ◽  
Gram Negative ◽  
Gram Positive Bacteria ◽  
Plasma Treatments

Non-thermal (low-temperature) physical plasma is under intensive study as an alternative approach to control superficial wound and skin infections when the effectiveness of chemical agents is weak due to natural pathogen or biofilm resistance. The purpose of this study was to test the individual susceptibility of pathogenic bacteria to non-thermal argon plasma and to measure the effectiveness of plasma treatments against bacteria in biofilms and on wound surfaces. Overall, Gram-negative bacteria were more susceptible to plasma treatment than Gram-positive bacteria. For the Gram-negative bacteria Pseudomonas aeruginosa, Burkholderia cenocepacia and Escherichia coli, there were no survivors among the initial 105 c.f.u. after a 5 min plasma treatment. The susceptibility of Gram-positive bacteria was species- and strain-specific. Streptococcus pyogenes was the most resistant with 17 % survival of the initial 105 c.f.u. after a 5 min plasma treatment. Staphylococcus aureus had a strain-dependent resistance with 0 and 10 % survival from 105 c.f.u. of the Sa 78 and ATCC 6538 strains, respectively. Staphylococcus epidermidis and Enterococcus faecium had medium resistance. Non-ionized argon gas was not bactericidal. Biofilms partly protected bacteria, with the efficiency of protection dependent on biofilm thickness. Bacteria in deeper biofilm layers survived better after the plasma treatment. A rat model of a superficial slash wound infected with P. aeruginosa and the plasma-sensitive Staphylococcus aureus strain Sa 78 was used to assess the efficiency of argon plasma treatment. A 10 min treatment significantly reduced bacterial loads on the wound surface. A 5-day course of daily plasma treatments eliminated P. aeruginosa from the plasma-treated animals 2 days earlier than from the control ones. A statistically significant increase in the rate of wound closure was observed in plasma-treated animals after the third day of the course. Wound healing in plasma-treated animals slowed down after the course had been completed. Overall, the results show considerable potential for non-thermal argon plasma in eliminating pathogenic bacteria from biofilms and wound surfaces.

scholarly journals Paper modified with ZnO nanorods – antimicrobial studies

2012 ◽  
Vol 3 ◽  
pp. 684-691 ◽  
Author(s):  
Mayuree Jaisai ◽  
Sunandan Baruah ◽  
Joydeep Dutta
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Aspergillus Niger ◽  
Zno Nanorods ◽  
Gram Negative Bacteria ◽  
Gram Positive ◽  
Gram Negative ◽  
Tissue Paper ◽  
Gram Positive Bacteria ◽  
Lighting Conditions

Paper with antimicrobial properties was developed through in situ growth of ZnO nanorods. The targeted application for this type of paper is in health centers as wallpaper, writing paper, facemasks, tissue paper, etc. The paper was tested on three model microbes, Gram-positive bacteriaStaphylococcus aureus,Gram-negative bacteriaEscherichia coliand common airborne fungusAspergillus niger. No viable bacterial colonies or fungal spores could be detected in the areas surrounding test samples of the antimicrobial paper. Gram-negative bacteriaEscherichia coliwere found to be inhibited in an area that is 239% and 163% the area of the paper sample under different room lighting conditions, i.e., halogen and fluorescent lamp illumination, respectively. For Gram-positive bacteriaStaphylococcus aureusthe zones of inhibition surrounding the paper samples are 102% and 70%, and forAspergillus niger, 224% and 183% of the sample area, under similar lighting conditions.

scholarly journals Correlation between Vancomycin MIC Values and Those of Other Agents against Gram-Positive Bacteria among Patients with Bloodstream Infections Caused by Methicillin-Resistant Staphylococcus aureus

2009 ◽  
Vol 53 (12) ◽  
pp. 5141-5144 ◽  
Author(s):  
N. Patel ◽  
P. Lubanski ◽  
S. Ferro ◽  
M. Bonafede ◽  
S. Harrington ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Tertiary Care Hospital ◽  
Tertiary Care ◽  
Bloodstream Infections ◽  
Care Hospital ◽  
Gram Positive ◽  
Methicillin Resistant ◽  
Gram Positive Bacteria ◽  
Included Patient

ABSTRACT An increase in the distribution of vancomycin MIC values among methicillin (meticillin)-resistant Staphylococcus aureus (MRSA) isolates has been noted. It is postulated that the shift in vancomycin MIC values may be associated with a concurrent rise in the MIC values of other anti-MRSA agents. Scant data are available on the correlation between vancomycin MIC values and the MIC values of other anti-MRSA agents. This study examined the correlation between vancomycin MIC values and the MIC values of daptomycin, linezolid, tigecycline, and teicoplanin among 120 patients with bloodstream infections caused by MRSA at a tertiary care hospital between January 2005 and May 2007. For each included patient, the MIC values of the antibiotics under study were determined by the Etest method and were separated into the following two categories: day 1 (index) and post-day 1 (subsequent). For subsequent isolates, the MIC values for each antibiotic from the post-day 1 terminal isolate were used. Among the index isolates, there was a significant correlation (P value, <0.01) between the MIC values for vancomycin and daptomycin and between the MIC values for vancomycin and teicoplanin. The MIC values for daptomycin were significantly correlated with linezolid, tigecycline, and teicoplanin MIC values. Among the 48 patients with subsequent isolates, vancomycin MIC values were significantly correlated with MIC values for daptomycin, linezolid, and teicoplanin (ρ value of ≥0.38 for all comparisons). This study documented an association between vancomycin MIC values and the MIC values of other anti-MRSA antibiotics among patients with bloodstream infections caused by MRSA primarily treated with vancomycin.

scholarly journals POTENSI ANTIMIKROBA DARI EKSTRAK DAN FRAKSI SPONS APLYSINA sp. TERHADAP MIKROBA UJI Escherichia coli, Staphylococcus aureus DAN Candida albicans

PHARMACON ◽  
2019 ◽  
Vol 8 (2) ◽  
pp. 268
Author(s):  
Marcelinda N. Kotel ◽  
Defny S. Wewengkang ◽  
Herny E. I. Simbala
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Candida Albicans ◽  
Marine Biota ◽  
Gram Negative Bacteria ◽  
Inhibitory Zone ◽  
Gram Positive ◽  
Gram Positive Bacteria ◽  
Inhibition Zones ◽  
Digital Caliper

ABSTRACT          Sponge  Aplysina  sp. is one of  the marine biota , which  has bioactive  compounds  that can be used  as medicinal ingredients. This study aims to determine the antimicrobial  potential of the extracts and fractions  of  sponge Aplysina  sp., against  microbes tested of  Escherichia coli, Staphylococcus aureus, and  Candida albicans. Aplysina  sp., sponge was extracted  using maceration method with  ethanol solvent and fractionated using methanol, n-hexan and chloroform solvents. To test  the antimicrobial  activity carried  out by disk diffusion agar method and observations carried out 24 hours incubation period, with inhibition zones measured using a digital caliper. The results showed that samples of  Aplysina  sp.,  proved to have antimicrobial compounds  to inhibit  Gram –positive bacteria  Staphylococcus aureus, and Gram- negative bacteria  Escherichia coli, with  the highest inhibitory  zone activity, and found  in Gram –positive Staphylococcus aureus bacteria   with measurements of 7,37 mm. Keywords: Sponge Aplysina  sp, Antimicrobial, Extraction,  Fractionation. ABSTRAK           Spons  Aplysina sp  merupakan salah satu  biota laut yang memiliki senyawa bioaktif yang dapat dijadikan  sebagai bahan obat. Penelitian ini bertujuan untuk mengetahui potensi antimikroba dari  Ekstrak dan Fraksi  Spons  Aplysina sp  Terhadap Mikroba Uji  Echerichia coli, Staphylococcus aureus, dan Candida albicans. Spons Aplysina sp diekstraksi  menggunakan metode maserasi dengan pelarut etanol dan difraksinasi  menggunakan  pelarut methanol, n-hexan, dan Kloroform. Untuk pengujian aktivitas antimikroba dilakukan dengan metode  difusi agar  dan  pengamatan dilakukan  1x24  jam  masa inkubasi,  dengan zona hambat diukur menggunakan  digital caliper. Hasil penelitian menunjukkan  bahwa sampel  Spons  Aplysina sp terbukti memiliki senyawa antimikroba untuk menghambat bakteri Gram positif  Staphylococcus aureus  dan  bakteri  Gram  negatif  Echerichia coli, dengan aktivitas zona hambat  tertinggi,  terdapat  pada  bakteri  Gram  positif   Staphylococcus  aureus  dengan hasil pengukuran  7,37 mm. Kata Kunci : Spons  Aplysina sp,  Antimikroba, Ekstraksi, Fraksinasi.

scholarly journals Synthesis, Characterization and Antimicrobial Activity Studies of Chlorocobaloximes with Neutral Bases Containing Amine Functionality

2021 ◽  
Vol 33 (11) ◽  
pp. 2781-2788
Author(s):  
V. Vijaikanth ◽  
S. Vasuki ◽  
C. Immanuel David ◽  
L. Chandran ◽  
S. Sowmya ◽  
...  
Keyword(s):  
Methicillin Resistant Staphylococcus Aureus ◽  
Spectroscopic Techniques ◽  
Gram Negative Bacteria ◽  
Zone Of Inhibition ◽  
Gram Positive ◽  
Gram Negative ◽  
Gram Positive Bacteria ◽  
Dimethyl Glyoxime ◽  
Uv Visible ◽  
Ethyl Amine

The inorganic cobaloximes of type [Co(Cl)(dmgH)2B]; where dmgH = dimethyl glyoxime and B = neutral bases: glycine, ethyl amine, 2-aminopyridine, 4-aminopyridine, 2,6-diaminopyridine, aniline and 1-napthylamine have been synthesized. The synthesized cobaloximes were characterized by IR, UV-visible, 1H NMR and 13C NMR spectroscopic techniques. The cobaloxime complexes were screened for their antibacterial activity against methicillin resistant Staphylococcus aureus (MRSA) and Acinetobacter baumannii by the zone of inhibition test, biofilm eradication on biomaterial using catheter and modified Congo red agar method. It has been found that the cobaloxime complexes exhibit inhibition against both gram positive and gram negative bacteria and the cobaloximes showed better inhibition towards Gram-negative bacteria compared to Gram-positive bacteria.

scholarly journals Categorization of Bacterial Pathogens Present in Infected Wounds and their Antibiotic Resistance Profile Recovered from Patients Attending Rizgary Hospital-Erbil

2021 ◽  
Vol 9 (2) ◽  
pp. 64-70
Author(s):  
Ahmed A. Al-Naqshbandi ◽  
Hedy A. Hassan ◽  
Mahmoud A. Chawsheen ◽  
Haval H. Abdul Qader
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Staphylococcus Epidermidis ◽  
Resistant Bacteria ◽  
Antibiotic Prescribing ◽  
Gram Negative Bacteria ◽  
Wound Infections ◽  
Gram Positive ◽  
Gram Negative ◽  
Gram Positive Bacteria

Wound infection with antibiotic-resistant bacteria can extend a patients’ debility and increase the expense of treatment in the long term; therefore, careful management of patients with wound infections is necessary to avoid complications. The usage of antimicrobial agent is a major factor in resistance development. This study aims to understand the causes of wound infections, as well as the criteria for diagnosing them for more sensible antibiotic prescribing. Samples from 269 wound patients were collected, and cultured for bacterial growth. Gram stain technique, bacterial identification via VITEK 2 compact system were investigated in this study. Gram negative bacteria accounted for 59.15% of the total isolates, while pathogenic gram positive bacteria accounted for 40.85% of total isolates. Escherichia coli and Pseudomonas aeruginosa are the dominant pathogenic gram negative bacteria in wounds, while Staphylococcus aureus, and Staphylococcus epidermidis are the dominant pathogenic gram positive bacteria. Pseudomonas aeruginosa showed 100% resistance to the majority of antibiotic tested, including Ampicillin, Amoxicillin/Clavulanic Acid, Aztreona, Ceftriaxone, and others. Staphylococcus aureus and Staphylococcus epidermidis are 100% resistant to Ampicillin, Ceftriaxone, and Cefotaxime. For more efficient antibiotic prescriptions, the causative microorganisms, and their current susceptibility patterns need to be mandated for testing before prescribing any antibiotics to patients. Prescriptions are frequently based solely on general information about the antibiotic's function, rather than on individual response variation to the pathogen and the antibiotic. Particularly when the common pathogens in this study show multidrug resistance in wounds.

scholarly journals Analysis of antibiotics administration in diabetic ulcer patients at Panembahan Senopati regional hospital Bantul

2021 ◽  
Vol 8 (8) ◽  
pp. 3782
Author(s):  
Adnal K. P. Husein Putra ◽  
Sri Sundari
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Foot Ulcer ◽  
Diabetic Foot Ulcer ◽  
Antibiotic Administration ◽  
Gram Negative Bacteria ◽  
Gram Positive ◽  
Diabetic Ulcer ◽  
Gram Negative ◽  
Gram Positive Bacteria

Background: This study provides an overview of diabetic ulcer infection, antibiotic susceptibility patterns, the most common types of antibiotics, factors influence in antibiotics administrations, and verifying the guideline used of antibiotics.Methods: The research used descriptive analysis combined with interviews. The study was starting from February-March 2021. Eighty-six diabetic ulcer patients were selected considering the inclusion and exclusion criteria. We interviewed seven doctors.Results: 71 bacterias were found with a gram-negative bacteria count of 80.3%- higher than gram-positive bacteria (19.7%). The most common gram-negative bacteria found are Escherichia coli (12.7%) and show the most heightened sensitivity with imipenem (80.7%), while cefuroxime and trimethoprim-sulfamethoxazole show the highest resistance (64.9%). The most common gram-positive bacteria found are Staphylococcus aureus (14.1%), and vancomycin shows the most heightened sensitivity (100%), while penicillin shows the highest resistance (71.4%). The most common single antibiotic administration was ceftriaxone, as well as a combination of two antibiotics, namely ceftriaxone and metronidazole.Conclusions: Escherichia coli was the most gram-negative bacteria, which has the highest sensitivity with imipenem, while Staphylococcus aureus was the most gram-positive bacteria, which has the most heightened sensitivity with vancomycin. The most common single antibiotic administration was ceftriaxone, as well as a combination of two antibiotics, ceftriaxone and metronidazole. Factors that influence antibiotics administration are patient condition, susceptibility testing, training, and advice from colleagues. Every doctor uses different guidelines for diabetic foot ulcer.

Sign in / Sign up

Export Citation Format

Share Document