scholarly journals Rapid TLC with Densitometry for Evaluation of Naproxen Stability

Processes ◽  
2020 ◽  
Vol 8 (8) ◽  
pp. 962
Author(s):  
Wioletta Parys ◽  
Małgorzata Dołowy ◽  
Alina Pyka-Pająk
Keyword(s):  
Silica Gel ◽  
Mobile Phase ◽  
Uv Light ◽  
Degradation Products ◽  
Low Cost ◽  
Cost Effective ◽  
Pharmaceutical Formulations ◽  
Good Alternative ◽  
Forced Degradation ◽  
Stress Studies

The purpose of the work was to develop such chromatographic conditions that allowed to separate as many naproxen degradation products as possible. In order to follow this process, thin-layer chromatography (TLC) coupled with densitometry and spectrodensitometry was used. A forced degradation study was performed using an ethanolic solution of naproxen spotted on silica gel plates, existing in the form of an aqueous solution at various pH values, and as solution prepared in saline and in hydrogen peroxide. Degradative effect of UV light on naproxen was watched in the context of naproxen spotted on plates precoated with silica gel and exposed to UV light, and also for its solution treated with UV light. However, the solution of naproxen prepared in water at pH ≈ 2.60 undergoes the largest changes as the results of its exposure to UV light during 10 h. Stressed samples of naproxen were analyzed by using a new and well validated TLC procedure including toluene (TOL)—acetone (ACE)—chloroform (CHL) (2:5:12, v/v/v) as mobile phase A and glacial acetic acid (AcOH)—n-hexane (Hex)—acetone (ACE)-(0.10:10:10, v/v/v) as mobile phase B. As the newly developed TLC-densitometric method can effectively separate the substances about pharmaceutical significance from products of its degradation, which are formed as a result of stress studies, is considered to be a good alternative and important tool in routine quality control and stability testing of naproxen in pharmaceutical formulations. These results indicate that proposed TLC-densitometric method is cost-effective, rapid, specific, accurate, and precise. This TLC procedure is comparable to HPLC and UPLC method in terms of detection the number of degradation products of naproxen. In addition, it realizes the criterion of linearity. A major advantage and novelty of proposed method is its low cost and ability to analyze examined drug and all degradation products simultaneously, including those which can be observed under intensive UV radiation exposure of naproxen solution which are not described by previous HPTLC studies available in the literature.

scholarly journals Simultaneous estimation of rosuvastatin and amlodipine in pharmaceutical formulations using stability indicating HPLC method

2014 ◽  
Vol 50 (3) ◽  
pp. 629-638 ◽  
Author(s):  
Muhammad Ashfaq ◽  
Tazeem Akhtar ◽  
Ghulam Mustafa ◽  
Muhammad Danish ◽  
Syed Naeem Razzaq ◽  
...  
Keyword(s):  
Mobile Phase ◽  
Degradation Products ◽  
Cost Effective ◽  
Pharmaceutical Formulations ◽  
Solvent Mixture ◽  
Hplc Method ◽  
Coefficient Of Determination ◽  
Simultaneous Estimation ◽  
Uv Detector ◽  
Ich Guideline

A viable cost-effective and isocratic approach employing C-18 column (250 mm × 4.6 mm, 5 µm) based HPLC has been utilized to separate and estimate the drugs, rosuvastatin, amlodipine and their stress induced degradation products, simultaneously in pharmaceutical formulations. Focused on ICH guideline parameters, the efficient separation of both drugs and their degradation products was achieved by optimizing a 30:70 (v/v) solvent mixture of acetonitrile and 0.1 M ammonium acetate buffer (pH 5) as mobile phase. The flow rate of the mobile phase was 1.5 mL/min and all the detections were carried out at 240 nm using UV detector. The method was linear in the concentration range of 1-200 µg/mL for rosuvastatin with 0.996 coefficient of determination value. For amlodipine, linearity was in the range of 0.5-100 µg/ml with 0.994 coefficient of determination value. Both the drugs along with their degradation products were separated in less than twenty minutes. The results of within-day and between-day precision were varied from 0.72 to 1.81% for rosuvastatin and 0.83 to 1.88% for amlodipine. The results show that this ICH validated method can be employed successfully for the routine as well as stability quantification of both the active ingredients simultaneously in pharmaceutical formulations.

scholarly journals All-Solid-State Potentiometric Ion-Sensors Based on Tailored Imprinted Polymers for Pholcodine Determination

Polymers ◽  
2021 ◽  
Vol 13 (8) ◽  
pp. 1192
Author(s):  
Hisham S. M. Abd-Rabboh ◽  
Abd El-Galil E. Amr ◽  
Abdulrahman A. Almehizia ◽  
Ayman H. Kamel
Keyword(s):  
Solid State ◽  
Low Cost ◽  
Cost Effective ◽  
Pharmaceutical Formulations ◽  
Measurement Sensitivity ◽  
Serum Samples ◽  
Multiwalled Carbon ◽  
Imprinted Polymers ◽  
High Measurement ◽  
Liquid Chromatographic

In recent times, the application of the use of ion-selective electrodes has expanded in the field of pharmaceutical analyses due to their distinction from other sensors in their high selectivity and low cost of measurement, in addition to their high measurement sensitivity. Cost-effective, reliable, and robust all-solid-state potentiometric selective electrodes were designed, characterized, and successfully used for pholcodine determination. The design of the sensor device was based on the use of a screen-printed electrode modified with multiwalled carbon nanotubes (MWCNTs) as a solid-contact transducer. Tailored pholcodine (PHO) molecularly imprinted polymers (MIPs) were prepared, characterized, and used as sensory receptors in the presented potentiometric sensing devices. The sensors exhibited a sensitivity of 31.6 ± 0.5 mV/decade (n = 5, R2 = 0.9980) over the linear range of 5.5 × 10−6 M with a detection limit of 2.5 × 10−7 M. Real serum samples in addition to pharmaceutical formulations containing PHO were analyzed, and the results were compared with those obtained by the conventional standard liquid chromatographic approach. The presented analytical device showed an outstanding efficiency for fast, direct, and low-cost assessment of pholcodine levels in different matrices.

Validated HPTLC Method for the Estimation of Oxetacaine in Pharmaceutical Formulations

2021 ◽  
pp. 5668-5672
Author(s):  
Kavitha J ◽  
Bonda Vismitha ◽  
Kokilambigai KS ◽  
Seetharaman R ◽  
Lakshmi KS
Keyword(s):  
Mobile Phase ◽  
Glacial Acetic Acid ◽  
Current Method ◽  
Cost Effective ◽  
Pharmaceutical Formulations ◽  
Relieve Pain ◽  
Ich Guidelines ◽  
Validation Parameters ◽  
Chromatographic Resolution ◽  
Hptlc Method

Oxetacaine is a potent local anesthetics used to relieve pain associated with peptic ulcer. The current method details about a rapid, accurate and precise HPTLC technique for the assessment of Oxetacaine in Pharmaceutical formulation. Chromatographic resolution was carried out on precoated HPTLC plates (Silica gel 60 F254 on Aluminum plate) employing methanol: water: glacial acetic acid (8: 1.8: 0.2 v/v/v) as mobile phase. Densitometric assessment was carried out at 220nm [Camag TLC Scanner III with winCATs software (version – 1.3.4)]. The drug was identified with a Rf value of 0.61. The reliability of the projected method was ascertained by evaluating various validation parameters as per ICH guidelines. The proposed technique can evaluate ten or more formulation units concurrently in a single run and affords a more rapid and cost-effective QC tool for regular analysis of Oxetacaine in pharmaceutical formulations.

scholarly journals Stability-Indicating Validated HPLC Method for Simultaneous Determination of Oral Antidiabetic Drugs from Thiazolidinedione and Sulfonylurea Groups in Combined Dosage Forms

2010 ◽  
Vol 93 (4) ◽  
pp. 1086-1092 ◽  
Author(s):  
Anna Gumieniczek ◽  
Anna Berecka ◽  
ukasz Komsta
Keyword(s):  
Simultaneous Determination ◽  
Mobile Phase ◽  
Uv Light ◽  
Degradation Products ◽  
Dosage Forms ◽  
Hplc Method ◽  
Base Temperature ◽  
Stability Indicating ◽  
Acid And Base

Abstract For type 2 diabetes treatment, combinations of drugs from the thiazolidinedione and sulfonylurea groups are now available in the same tablet or capsule. Therefore, a stability-indicating and validated HPLC method was developed for simultaneous determination of pioglitazone, rosiglitazone, and glipizide in combined dosage forms. The examined drugs were subjected to different conditions such as acid and base, temperature, and UV light, and degradation of pioglitazone and glipizide was observed under thermal and acidic stress. However, selectivity of the presented method for pioglitazone, rosiglitazone, and glipizide assay against their degradation products was confirmed. It was also demonstrated to be robust, resisting small deliberate changes in pH of the buffer, flow rate, and percentage of acetonitrile in the mobile phase. The presented method utilizes a LiChrospher RP18 column (125 4.0 mm), acetonitrile in phosphate buffer at pH 4.3 (40 + 60, v/v) as the mobile phase, and UV detection at 225 nm for pioglitazone/glipizide or 245 nm for rosiglitazone/glipizide. The method was validated with respect to linearity, precision, and accuracy. Finally, the elaborated procedure was applied for the QC of pioglitazone/glipizide and rosiglitazone/glipizide mixtures.

scholarly journals Determination of Delafloxacin in Pharmaceutical Formulations Using a Green RP-HPTLC and NP-HPTLC Methods: A Comparative Study

Antibiotics ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 359 ◽  
Author(s):  
Prawez Alam ◽  
Essam Ezzeldin ◽  
Muzaffar Iqbal ◽  
Gamal A.E. Mostafa ◽  
Md. Khalid Anwer ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Silica Gel ◽  
Solid Lipid Nanoparticles ◽  
Ternary Mixture ◽  
Mobile Phase ◽  
Pharmaceutical Formulations ◽  
Lipid Nanoparticles ◽  
Ammonia Solution ◽  
Hptlc Method

In this work; delafloxacin (DLFX) was determined using a validated green RP-HPTLC and NP-HPTLC methods in commercial tablets and in-house developed solid lipid nanoparticles (SLNs). RP-HPTLC determination of DLFX was performed using “RP-18 silica gel 60 F254S HPTLC plates”. However; NP-HPTLC estimation of DLFX was performed using “silica gel 60 F254S HPTLC plates”. For a green RP-HPTLC method; the ternary combination of ethanol:water:ammonia solution (5:4:2 v/v/v) was used as green mobile phase. However; for NP-HPTLC method; the ternary mixture of ethyl acetate: methanol: ammonia solution (5:4:2 v/v/v) was used as normal mobile phase. The analysis of DLFX was conducted in absorbance/reflectance mode of densitometry at λmax = 295 nm for both methods. RP-HPTLC method was found more accurate, precise, robust and sensitive for the analysis of DLFX compared with the NP-HPTLC method. The % assay of DLFX in commercial tablets and in-house developed SLNs was determined as 98.2 and 101.0%, respectively, using the green RP-HPTLC technique, however; the % assay of DLFX in commercial tablets and in-house developed SLNs was found to be 94.4 and 95.0%, respectively, using the NP-HPTLC method. Overall, the green RP-HPTLC method was found superior over the NP-HPTLC. Therefore, the proposed green RP-HPTLC method can be successfully applied for analysis of DLFX in commercial tablets, SLNs and other formulations containing DLFX.

Stability-Indicating Methods for the Determination of Candesartan Cilexetil in Bulk Drug and Pharmaceutical Formulations

2013 ◽  
Vol 96 (3) ◽  
pp. 580-586 ◽  
Author(s):  
Yousry M Issa ◽  
Emad M Hussien ◽  
Magda M Ibrahim ◽  
Fatma M Abdel-Gawad ◽  
Saadia Barakat
Keyword(s):  
Mobile Phase ◽  
Candesartan Cilexetil ◽  
Degradation Products ◽  
Pharmaceutical Formulations ◽  
Stability Indicating ◽  
Bulk Powder ◽  
System Suitability ◽  
The Mean ◽  
Bulk Drug

Abstract Two stability-indicating methods were developed for the determination of candesartan cilexetil in the presence of its degradation products. The first method uses isocratic RP-HPLC with an Agilent C18 column. The mobile phase was phosphate buffer (pH = 2.8 ± 0.1)–acetonitrile (60 + 40, v/v). The flow rate was 2.0 mL/min, and the UV detection was at 254 nm. The second method depends on TLC-densitometric measurements of drug spots at 254 nm. The separation was carried out on silica gel 60 F254 plates using ethyl acetate–methanol–toluene– ammonia 33% (40 + 25 + 20 + 2, v/v/v/v) mobile phase. The methods were validated according to U.S. Pharmacopeia guidelines, and the acceptance criteria for accuracy, precision, linearity, specificity, robustness, LOD, LOQ, and system suitability were met in all cases. Linear ranges of the methods were 10.0–200.0 μg/mL and 1.0–9.0 μg/spot for HPLC and TLC, respectively. The proposed methods were successfully applied to the drug in bulk powder, in laboratory-prepared mixtures with its degradation products, and in commercially available tablets. The results were compared statistically at the 95% confidence level with each other. There were no significant differences between the mean recovery and precision of the two methods.

scholarly journals Stability Indicating HPLC Determination of Risperidone in Bulk Drug and Pharmaceutical Formulations

2011 ◽  
Vol 2011 ◽  
pp. 1-6 ◽  
Author(s):  
Zarna R. Dedania ◽  
Ronak R. Dedania ◽  
Navin R. Sheth ◽  
Jigar B. Patel ◽  
Bhavna Patel
Keyword(s):  
Limit Of Detection ◽  
Degradation Products ◽  
Pharmaceutical Formulations ◽  
Assay Method ◽  
Pharmaceutical Dosage Forms ◽  
Stability Indicating ◽  
Stress Studies ◽  
Liquid Chromatographic Separation ◽  
Bulk Drug ◽  
Liquid Chromatographic

The objective of the current study was to develop a validated stability-indicating assay method (SIAM) for risperidone after subjecting it to forced decomposition under hydrolysis, oxidation, photolysis, and thermal stress conditions. The liquid chromatographic separation was achieved isocratically on a symmetry C18 column (5 μm size, 250 mm × 4.6 mm i.d.) using a mobile phase containing methanol: acetonitrile (80 : 20, v/v) at a flow rate of 1 mL/min and UV detection at 280 nm. Retention time of risperidone was found to be . The method was linear over the concentration range of 10–60 μg/mL with a limit of detection and quantitation of 1.79 and 5.44 μg/mL, respectively. The method has the requisite accuracy, specificity, sensitivity, and precision to assay risperidone in bulk form and pharmaceutical dosage forms. Degradation products resulting from the stress studies did not interfere with the detection of Risperidone, and the assay is thus stability indicating.

scholarly journals Cost-Effective Disinfection Robot Using UVC Radiation

2021 ◽  
Vol 40 ◽  
pp. 02003
Author(s):  
Mansi Dhikle ◽  
Vinaya Dharne ◽  
Pankaja Gaikar ◽  
Kausar Fakir
Keyword(s):  
Video Streaming ◽  
Uv Light ◽  
Low Cost ◽  
Cost Effective ◽  
Live Video ◽  
Medical Facilities ◽  
Ultraviolet C ◽  
Cleaning Robots ◽  
Uv C ◽  
Uvc Radiation

Sanitization with human efforts is not an easy task. Chances of contracting infections increases which leads to additional spread of bacteria. Currently, normal cleaning robots are used in most of the places but looking at the current situation the sanitization techniques need to be improved. The robot uses radiation of UV rays to kill the microrganisms. It gives a live video streaming of its surrounding using a Wi-fi based camera. With the help of Bluetooth module and android mobile, we can control the movement of the robot inside the room without being physically present. It is built with PIC Microcontroller and Ultraviolet-C (UVC) Sanitization LED. UV-C has bandwidth range of 200-280nm and is most powerful when it comes to killing pathogens in the room. This allows us to sterilise the room effectively. By killing the germs, the UV light restricts their multiplication by destroying their reproductive system. Thus use of this robot lowers the threat of infection, cost of traditional cleaning and sterilisation and increases security in medical facilities. Thus, we are trying to implement a more efficient way of sanitization by building a Low cost UV sanitization Robot which can be used in small clinics and for household purpose.

scholarly journals PENETAPAN RHODAMIN B PADA SAMPEL LIPSTIK DENGAN MENGGUNAKAN KLT-SPEKTROFOTODENSITOMETRI

Jurnal Kimia ◽  
2020 ◽  
pp. 77
Author(s):  
N.N.A.S. Devi ◽  
N.P.M.P.P. Winarni ◽  
I.P. Priyasana ◽  
G.A.D. Mayagita ◽  
V. Rahmadinha ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Silica Gel ◽  
Liver Damage ◽  
Rhodamine B ◽  
Mobile Phase ◽  
Uv Light ◽  
Lymph Glands ◽  
Red Color ◽  
Rhodamine B Dye ◽  
Rhodamin B

Lipsticks are widely used by women to beautify themselves. Among the various colors that make lipstick more interesting, red color lipstick is the most demanded one. Rhodamine B is a synthetic dye that banned for use and certified as a hazardous material according to Minister of Health of Indonesian Republic No. 376/Menkes/Per/1990 because it causes liver damage, kidney and lymph glands damage, followed with organ enlargement. The aim of this study is to identify Rhodamine B in lipsticks in the market. Samples were taken from 3 shops in Denpasar City and Badung Regency. Samples were soaked with amonia solution with using wool yarn to extract the rhodamine B dye and identification using TLC plate silica gel GF254 withn-butanol: ethyl acetate: amonia (1322:5.2:6.5) as mobile phase then detected with UV light 254 and 366 nm. Identification by spectrophotodensitometry where the TLC plate was observed in the TLC Analyzer to observe the AUC in each spot formed. The AUC obtained from the instrument illustrates the concentration of the analyte in each bottle. The result shows that 3 examined samples doesn’t contain rhodamine B.   Keywords: Rhodamin B, Lipstik, TLC, Spectrophotodensitometry

scholarly journals Electrochemical biosensors in pharmaceutical analysis

2010 ◽  
Vol 46 (3) ◽  
pp. 375-391 ◽  
Author(s):  
Eric de Souza Gil ◽  
Giselle Rodrigues de Melo
Keyword(s):  
Pharmaceutical Analysis ◽  
Active Component ◽  
Electrochemical Sensors ◽  
Degradation Products ◽  
Biological Fluids ◽  
Low Cost ◽  
Analytical Techniques ◽  
Pharmaceutical Formulations ◽  
Quality Analysis ◽  
Increasing Demand

Given the increasing demand for practical and low-cost analytical techniques, biosensors have attracted attention for use in the quality analysis of drugs, medicines, and other analytes of interest in the pharmaceutical area. Biosensors allow quantification not only of the active component in pharmaceutical formulations, but also the analysis of degradation products and metabolites in biological fluids. Thus, this article presents a brief review of biosensor use in pharmaceutical analysis, focusing on enzymatic electrochemical sensors.

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