scholarly journals Gellan Gum Hydrogels Filled Edible Oil Microemulsion for Biomedical Materials: Phase Diagram, Mechanical Behavior, and In Vivo Studies

Polymers ◽  
2021 ◽  
Vol 13 (19) ◽  
pp. 3281
Author(s):  
Muhammad Zulhelmi Muktar ◽  
Muhammad Ameerul Amin Bakar ◽  
Khairul Anuar Mat Amin ◽  
Laili Che Rose ◽  
Wan Iryani Wan Ismail ◽  
...  
Keyword(s):  
Phase Diagram ◽  
Thermal Behavior ◽  
Chemical Interaction ◽  
Ternary Phase Diagram ◽  
Transmission Rate ◽  
Wound Care ◽  
Gellan Gum ◽  
In Vivo Studies ◽  
Histological Evaluation

The demand for wound care products, especially advanced and active wound care products is huge. In this study, gellan gum (GG) and virgin coconut oil (VCO) were utilized to develop microemulsion-based hydrogel for wound dressing materials. A ternary phase diagram was constructed to obtain an optimized ratio of VCO, water, and surfactant to produce VCO microemulsion. The VCO microemulsion was incorporated into gellan gum (GG) hydrogel (GVCO) and their chemical interaction, mechanical performance, physical properties, and thermal behavior were examined. The stress-at-break (σ) and Young’s modulus (YM) of GVCO hydrogel films were increased along with thermal behavior with the inclusion of VCO microemulsion. The swelling degree of GVCO hydrogel decreased as the VCO microemulsion increased and the water vapor transmission rate of GVCO hydrogels was comparable to commercial dressing in the range of 332–391 g m−2 d−1. The qualitative antibacterial activities do not show any inhibition against Gram-negative (Escherichia coli and Klebsiella pneumoniae) and Gram-positive (Staphylococcus aureus and Bacillus subtilis) bacteria. In vivo studies on Sprague–Dawley rats show the wound contraction of GVCO hydrogel is best (95 ± 2%) after the 14th day compared to a commercial dressing of Smith and Nephew Opsite post-op waterproof dressing, and this result is supported by the ultrasound images of wound skin and histological evaluation of the wound. The findings suggest that GVCO hydrogel has the potential to be developed as a biomedical material.

scholarly journals Rifampicin-Loaded Alginate-Gelatin Fibers Incorporated within Transdermal Films as a Fiber-in-Film System for Wound Healing Applications

Membranes ◽  
2020 ◽  
Vol 11 (1) ◽  
pp. 7
Author(s):  
Ameya Sharma ◽  
Vivek Puri ◽  
Pradeep Kumar ◽  
Inderbir Singh
Keyword(s):  
Wound Healing ◽  
Transmission Rate ◽  
Wound Care ◽  
Healing Process ◽  
In Vivo Studies ◽  
Wound Healing Process ◽  
Uptake Study ◽  
Tissue Proliferation ◽  
Water Transmission

The various biological and molecular cascades including different stages or phases such as inflammation, tissue proliferation, and remodeling phases, which significantly define the wound healing process. The natural matrix system is suggested to increase and sustain these cascades. Biocompatible biopolymers, sodium alginate and gelatin, and a drug (Rifampicin) were used for the preparation of fibers into a physical crosslinking solution using extrusion-gelation. The formed fibers were then loaded in transdermal films for wound healing applications. Rifampicin, an antibiotic, antibacterial agent was incorporated into fibers and afterwards the fibers were loaded into transdermal films. Initially, rifampicin fibers were developed using biopolymers including alginate and gelatin, and were further loaded into polymeric matrix which led to the formation of transdermal films. The transdermal films were coded as TF1, TF2, TF3 and TF4.The characterization technique, FTIR, was used to describe molecular transitions within fibers, transdermal films, and was further corroborated using SEM and XRD. In mechanical properties, the parameters, such as tensile strength and elongation-at-break (extensibility), were found to be ranged between 2.32 ± 0.45 N/mm2 to 14.32 ± 0.98 N/mm2 and 15.2% ± 0.98% to 30.54% ± 1.08%. The morphological analysis firmed the development of fibers and fiber-loaded transdermal films. Additionally, physical evaluation such as water uptake study, water transmission rate, swelling index, moisture content, and moisture uptake study were executed to describe comparative interpretation of the formulations developed. In vivo studies were executed using a full thickness cutaneous wound healing model, the transdermal films developed showed higher degree of contraction, i.e., 98.85% ± 4.04% as compared to marketed formulation (Povidone). The fiber-in-film is a promising delivery system for loading therapeutic agents for effective wound care management.

scholarly journals Study of Melatonin as Preventive Agent of Gastrointestinal Damage Induced by Sodium Diclofenac

Cells ◽  
2020 ◽  
Vol 9 (1) ◽  
pp. 180 ◽  
Author(s):  
Aroha B. Sánchez ◽  
Beatriz Clares ◽  
María J. Rodríguez-Lagunas ◽  
María J. Fábrega ◽  
Ana C. Calpena
Keyword(s):  
Side Effects ◽  
Redox State ◽  
Ex Vivo ◽  
Inhibitory Effect ◽  
Experimental Models ◽  
In Vivo Studies ◽  
Histological Evaluation ◽  
Sodium Diclofenac ◽  
Cox 2

Safety profile of nonsteroidal anti-inflammatory drugs (NSAIDs) has been widely studied and both therapeutic and side effects at the gastric and cardiovascular level have been generally associated with the inhibitory effect of isoform 1 (COX-1) and 2 (COX-2) cyclooxygenase enzymes. Now there are evidences of the involvement of multiple cellular pathways in the NSAIDs-mediated-gastrointestinal (GI) damage related to enterocyte redox state. In a previous review we summarized the key role of melatonin (MLT), as an antioxidant, in the inhibition of inflammation pathways mediated by oxidative stress in several diseases, which makes us wonder if MLT could minimize GI NSAIDs side effects. So, the aim of this work is to study the effect of MLT as preventive agent of GI injury caused by NSAIDs. With this objective sodium diclofenac (SD) was administered alone and together with MLT in two experimental models, ex vivo studies in pig intestine, using Franz cells, and in vivo studies in mice where stomach and intestine were studied. The histological evaluation of pig intestine samples showed that SD induced the villi alteration, which was prevented by MLT. In vivo experiments showed that SD altered the mice stomach mucosa and induced tissue damage that was prevented by MLT. The evaluation by quantitative reverse transcription PCR (RT-qPCR) of two biochemical markers, COX-2 and iNOS, showed an increase of both molecules in less injured tissues, suggesting that MLT promotes tissue healing by improving redox state and by increasing iNOS/NO that under non-oxidative condition is responsible for the maintenance of GI-epithelium integrity, increasing blood flow and promoting angiogenesis and that in presence of MLT, COX-2 may be responsible for wound healing in enterocyte. Therefore, we found that MLT may be a preventive agent of GI damages induced by NSAIDs.

Gellan gum blended PEI nanocomposites as gene delivery agents: Evidences from in vitro and in vivo studies

2011 ◽  
Vol 79 (1) ◽  
pp. 3-14 ◽  
Author(s):  
Ritu Goyal ◽  
S.K. Tripathi ◽  
Shilpa Tyagi ◽  
K. Ravi Ram ◽  
K.M. Ansari ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Gellan Gum ◽  
In Vivo Studies

Use of a Maggot Motility Index to Evaluate Survival of Therapeutic Larvae

2004 ◽  
Vol 94 (4) ◽  
pp. 353-355 ◽  
Author(s):  
Anthony Rosales ◽  
Jefferey R. Vazquez ◽  
Brian Short ◽  
Heather R. Kimbriel ◽  
Matthew J. Claxton ◽  
...  
Keyword(s):  
Room Temperature ◽  
Wound Care ◽  
In Vivo Studies ◽  
Clinical Use ◽  
Short Term ◽  
Motility Index ◽  
Term Storage ◽  
Over Time ◽  
Surrogate Method

Maggot debridement therapy is rapidly increasing in popularity at major diabetic foot and wound care centers worldwide. However, we are unaware of specific guidelines on the short-term storage of larvae. We sought to evaluate differences in maggot motility over time in larvae refrigerated versus those stored at room temperature. We also introduce a simple surrogate method for evaluating maggot vitality that may be useful for in vivo studies if validated in future works. We randomly selected ten larvae from the same shipment at ten different times in 9 days. Larvae were placed on a translucent acetate grid, and their total excursion in 30 sec was measured. This was converted into a Maggot Motility Index. In the refrigerated group, the index remained at or above 40 mm/min for approximately 60 hours from baseline, when there was a significant decrease. This same phenomenon occurred during the first 12 hours in the nonrefrigerated group. There were significant differences in motility between refrigerated and nonrefrigerated larvae immediately after baseline until day 8. Larvae are more practical for repeated clinical use if kept refrigerated between applications. (J Am Podiatr Med Assoc 94(4): 353–355, 2004)

Scaffolds Materials from Gellan Gum Incorporated Ball Clay as Dressing Materials

2021 ◽  
Vol 1023 ◽  
pp. 83-88
Author(s):  
Nur Masyitah Hamdan ◽  
Khairul Anuar Mat Amin
Keyword(s):  
Thermal Behavior ◽  
Mechanical Performance ◽  
Wound Care ◽  
Compressive Strain ◽  
Gellan Gum ◽  
Wound Management ◽  
Potential Candidate ◽  
Swelling Properties ◽  
Ball Clay ◽  
Performance Results

The demand for wound management treatment especially advanced and active wound care products is huge. In this study, the scaffolds were prepared from gellan gum (GG) incorporated ball clay (BC) at different concentrations to investigate their swelling properties, water vapor transmission rates (WVTR), mechanical characteristic and thermal behavior. There are three different concentrations of BC were added into the GG scaffolds which were 5% w/w (GG/BC5), 10% w/w (GG/BC10) and 15% w/w (GG/BC15). Swelling ratio of GG scaffolds were increased upon addition of ball clay, while WVTR values of all scaffolds were decreased in the range of 1081–1164 g m−2 d−1. The mechanical performance results show that the GG/BC10 has the highest compressive stress at break (26 ± 5 MPa) and compressive strain at break (110 ± 21%). For thermal behavior, it shows that the thermal stability of GG scaffolds had improved after the addition of ball clay attributed to the interaction between GG and ball clay. The results show that the GG/BC scaffolds could be a potential candidate to be used as an active wound care product.

Gellan gum and its methacrylated derivatives as in situ gelling mucoadhesive formulations of pilocarpine: In vitro and in vivo studies

2020 ◽  
Vol 577 ◽  
pp. 119093 ◽  
Author(s):  
Laura E. Agibayeva ◽  
Daulet B. Kaldybekov ◽  
Natalia N. Porfiryeva ◽  
Venera R. Garipova ◽  
Rauash A. Mangazbayeva ◽  
...  
Keyword(s):  
Gellan Gum ◽  
In Vivo Studies ◽  
In Situ Gelling

Development of floating in situ gelling system as an efficient anti-ulcer formulation: in vitro and in vivo studies

RSC Advances ◽  
2015 ◽  
Vol 5 (37) ◽  
pp. 28848-28856 ◽  
Author(s):  
C. Bothiraja ◽  
Vijay Kumbhar ◽  
Atmaram Pawar ◽  
Karimunnisa Shaikh ◽  
Ravindra Kamble
Keyword(s):  
Calcium Carbonate ◽  
Gellan Gum ◽  
In Vivo Studies ◽  
Model Drug ◽  
In Situ Gelling

The aim of the present work was to design gellan gum and calcium carbonate based floating in situ gel as an efficient anti-ulcer formulation using andrographolide (AG) as a model drug.

scholarly journals Fabrication and Optimization of Novel Lornoxicam Matrix Tablets Using 3-Factor 3- Level Box-Behnken Statistical Design: Invitro and Invivo Evaluation

2014 ◽  
Vol 2 (02) ◽  
pp. 87-95
Author(s):  
A. Iqbal ◽  
R. M. Sarfraz ◽  
A. Mahmood ◽  
H. Ahsan ◽  
M. Zaman ◽  
...  
Keyword(s):  
Drug Release ◽  
Sustained Release ◽  
Chemical Interaction ◽  
Methyl Cellulose ◽  
Statistical Design ◽  
Matrix Tablets ◽  
In Vivo Studies ◽  
Release Mechanism ◽  
Polynomial Models

In the present study efforts have been made to prepare sustained release matrix tablets of Lornoxicam. Matrix tablets were prepared by direct compression method by using Hydroxypropyl methyl cellulose K15 (HPMC- K15), Ethyl cellulose (EC) and Sodium carboxy methyl cellulose (Na-CMC) as polymers in different concentrations. A 3-factor 3- level Box-Behnken statistical design was used as an optimization tool having total of 17 experimental runs with 5 central points. All three polymers were selected as independent variables while %age drug release at various time intervals and hardness were used as dependant variables. In vivo studies were conducted on human plasma using Tenoxicam as internal standered. All the detections were made on SYKNM HPLC. Foriour Transform Infrared Spectroscopy (FTIR) and Differential Scanning Calorimetery (DSC) studies were conducted and no chemical interaction was found between drug and polymers. The drug release mechanism was mainly governed by non-fickian (anomalous) diffusion and zero-order (case II) transport diffusion. Regression analysis was performed on dissolution data obtained with the selected response variables and polynomial models were constructed. Polynomial models were further validated using one way ANOVA and results indicated that all the polymers used have significant effect on selected response (p>0.05). Contour plots and three dimensional response surface curves were drawn. In- vivo studies were conducted on two tablet formulation indicating slow and sustained release of the drug from matrix. From Behnken design it is possible to successfully formulate and optimize Lornoxicam sustained release matrix tablets with three polymers (HPMC- K15, EC and Na-CMC) in combination.

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