scholarly journals Synthesis and Characterization of Porous, Electro-Conductive Chitosan–Gelatin–Agar-Based PEDOT: PSS Scaffolds for Potential Use in Tissue Engineering

Polymers ◽  
2021 ◽  
Vol 13 (17) ◽  
pp. 2901 ◽  
Author(s):  
Dania Adila Ahmad Ruzaidi ◽  
Mohd Muzamir Mahat ◽  
Zarif Mohamed Sofian ◽  
Nikman Adli Nor Hashim ◽  
Hazwanee Osman ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Electrochemical Impedance ◽  
Synthesis And Characterization ◽  
X Ray Diffraction ◽  
Ftir Characterization ◽  
Phosphate Buffered Saline ◽  
In Vitro Stability ◽  
Potential Use

Herein we report the synthesis and characterization of electro-conductive chitosan–gelatin–agar (Cs-Gel-Agar) based PEDOT: PSS hydrogels for tissue engineering. Cs-Gel-Agar porous hydrogels with 0–2.0% (v/v) PEDOT: PSS were fabricated using a thermal reverse casting method where low melting agarose served as the pore template. Sample characterizations were performed by means of scanning electron microscopy (SEM), attenuated total reflectance–Fourier transform infrared spectroscopy (ATR–FTIR), X-ray diffraction analysis (XRD) and electrochemical impedance spectroscopy (EIS). Our results showed enhanced electrical conductivity of the cs-gel-agar hydrogels when mixed with DMSO-doped PEDOT: PSS wherein the optimum mixing ratio was observed at 1% (v/v) with a conductivity value of 3.35 × 10−4 S cm−1. However, increasing the PEDOT: PSS content up to 1.5 % (v/v) resulted in reduced conductivity to 3.28 × 10−4 S cm−1. We conducted in vitro stability tests on the porous hydrogels using phosphate-buffered saline (PBS) solution and investigated the hydrogels’ performances through physical observations and ATR–FTIR characterization. The present study provides promising preliminary data on the potential use of Cs-Gel-Agar-based PEDOT: PSS hydrogel for tissue engineering, and these, hence, warrant further investigation to assess their capability as biocompatible scaffolds.

scholarly journals Synthesis and Characterization of Silver-Coated Polymeric Scaffolds for Bone Tissue Engineering: Antibacterial and In Vitro Evaluation of Cytotoxicity and Biocompatibility

ACS Omega ◽  
2021 ◽  
Vol 6 (6) ◽  
pp. 4335-4346
Author(s):  
Muhammad Umar Aslam Khan ◽  
Saiful Izwan Abd Razak ◽  
Hassan Mehboob ◽  
Mohammed Rafiq Abdul Kadir ◽  
T. Joseph Sahaya Anand ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Synthesis And Characterization ◽  
In Vitro Evaluation ◽  
Polymeric Scaffolds

Preparation and Characterization of Akermanite/Merwinite Scaffolds for Bone Tissue Repair

2020 ◽  
Vol 44 ◽  
pp. 73-81
Author(s):  
Mojtaba Ansari ◽  
Farzad Malmir ◽  
Amir Salati
Keyword(s):  
Bone Tissue ◽  
Bone Marrow Stromal Cells ◽  
Sol Gel ◽  
Magnesium Silicate ◽  
X Ray Diffraction ◽  
X Ray ◽  
Calcium Magnesium ◽  
Potential Use

The ceramics in the system CaO–MgO–SiO2 has recently attracted a great deal of attention because they display a good in vitro bioactivity and have potential use as bone implants. Biphasic calcium-magnesium-silicate ceramics were prepared by a sol-gel method. The dried gel with chemical composition 3CaO.MgO.2SiO2 was thermally treated at 1200 °C for 2 hrs. The structural behavior of the synthesized ceramics was examined by means of X-ray diffraction (XRD), Fourier Transform Infrared Spectroscopy (FTIR) and Scanning Electron Microscopy (SEM). Merwinite crystalline phase and akermanite phase were recognized. Then, porous akermanite/merwinite scaffolds were prepared to utilize polymer sponge method and evaluated by employing SEM. Furthermore, bone marrow stromal cells (BMSC) adhesion and proliferation on the scaffolds were evaluated by MTT assay test. Differentiation of the cells was assessed by measuring alkaline phosphatase (ALP) activity. The results demonstrated that BMSC adhered and spread well on akermanite scaffolds and proliferated with the increase in the culture time, and the differentiation rate of osteoblasts on scaffolds was comparable to that on blank culture plate control. Thus, the obtained results presented that the akermanite/merwinite scaffolds deserve attention for bone tissue engineering applications.

scholarly journals Activity of Pt(II) and Ru(III) Triazolopyrimidine Complexes Against Parasites of the Genus Leishmania, Trypanosomas and Phytomonas

2001 ◽  
Vol 8 (3) ◽  
pp. 119-124 ◽  
Author(s):  
Juan M. Salas ◽  
Miguel Quirós ◽  
Mohammad Abul Haj ◽  
Rosa Magán ◽  
Clotilde Marín ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Synthesis And Characterization ◽  
In Vitro Activity ◽  
X Ray Diffraction ◽  
Procyclic Form ◽  
X Ray ◽  
Promastigote Form ◽  
Genus Leishmania

The synthesis and characterization of two Pt(II) Complexes with the isomeric ligands 4,5-dihydro-5-oxo- [1,2,4]triazolo-[ 1,5-a]pyrimidine (5HtpO) and 4,7-dihydro-7-oxo-[ 1,2,4]-triazolo-[ 1,5-a]pyrimidine (7HtpO) are described, as well as a Ru(III) complex with 7HtpO. The crystal structure of cis-[PtCl2(7HtpO)2].2H2O has been solved by X-ray diffraction analysis. In vitro activity of the new isolated complexes against the epimastigote form of T. cruzi, procyclic form of T. b. brucei and promastigote form of L. donnovani and P. characias has also been studied. The three complexes markedly affect the growth of the parasites and none of them shows cytotoxicity against macrophage of the J774.2 line at the heaviest dosages used.

Rhenium-188 Labeled Hydroxyapatite and Rhenium-188 Sulfur Colloid

1997 ◽  
Vol 36 (02) ◽  
pp. 71-75 ◽  
Author(s):  
S. Glatz ◽  
S. N. Reske ◽  
K. G. Grillenberger
Keyword(s):  
Synovial Fluid ◽  
Ph Value ◽  
Surgical Removal ◽  
Radiation Synovectomy ◽  
Sulfur Colloid ◽  
Target Organs ◽  
Aseptic Conditions ◽  
In Vitro Stability ◽  
Potential Use

Summary Aim: One therapeutic approach to rheumatoid arthritis and other inflammatory arthropathies besides surgical removal of inflamed synovium is radiation synovectomy using beta-emitting radionuclides to destroy the affected synovial tissue. Up to now the major problem associated with the use of labeled particles or colloids has been considerable leakage of radionuclides from the injected joint coupled with high radiation doses to liver and other non target organs. In this study we compared 188Re labeled hydroxyapatite particles and 188Re rhenium sulfur colloid for their potential use in radiation synovectomy. Methods: To this end we varied the labeling conditions (concentrations, pH-value, heating procedure) and analyzed the labeling yield, radiochemical purity, and in vitro stability of the resulting radiopharmaceutical. Results: After optimizing labeling conditions we achieved a labeling yield of more than 80% for 188Re hydroxyapatite and more than 90% for the rhenium sulfur colloid. Both of the radiopharmaceuticals can be prepared under aseptic conditions using an autoclav for heating without loss of activity. In vitro stability studies using various challenge solutions (water, normal saline, diluted synovial fluid) showed that 188Re labeled hydroxyapatite particles lost about 80% of their activity within 5 d in synovial fluid. Rhenium sulfur colloid on the other hand proved to be very stable with a remaining activity of more than 93% after 5 d in diluted synovial fluid. Conclusion: These in vitro results suggest that 188Re labeled rhenium sulfur colloid expects to be more suitable for therapeutic use in radiation synovectomy than the labeled hydroxyapatite particles.

Synthesis and characterization of TiN / Ti / AISI 410 coatings

2018 ◽  
Vol 2 (1) ◽  
pp. 7
Author(s):  
S Chirino ◽  
Jaime Diaz ◽  
N Monteblanco ◽  
E Valderrama
Keyword(s):  
Synthesis And Characterization ◽  
Photoemission Spectroscopy ◽  
X Ray Diffraction ◽  
X Ray ◽  
Columnar Grains ◽  
Tin Thin Films ◽  
Steel Aisi ◽  
Interacting Surfaces ◽  
Stainless Steel Aisi

The synthesis and characterization of Ti and TiN thin films of different thicknesses was carried out on a martensitic stainless steel AISI 410 substrate used for tool manufacturing. The mechanical parameters between the interacting surfaces such as thickness, adhesion and hardness were measured. By means of the scanning electron microscope (SEM) the superficial morphology of the Ti/TiN interface was observed, finding that the growth was of columnar grains and by means of EDAX the existence of titanium was verified.  Using X-ray diffraction (XRD) it was possible to observe the presence of residual stresses (~ -3.1 GPa) due to the different crystalline phases in the coating. Under X-ray photoemission spectroscopy (XPS) it was possible to observe the molecular chemical composition of the coating surface, being Ti-N, Ti-N-O and Ti-O the predominant ones.

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