scholarly journals Evaluation of 3D-Printing Scaffold Fabrication on Biosynthetic Medium-Chain-Length Polyhydroxyalkanoate Terpolyester as Biomaterial-Ink

Polymers ◽  
2021 ◽  
Vol 13 (14) ◽  
pp. 2222
Author(s):  
Anuchan Panaksri ◽  
Nuttapol Tanadchangsaeng
Keyword(s):  
Tissue Engineering ◽  
Soft Tissue ◽  
Chain Length ◽  
Cell Attachment ◽  
Carbon Sources ◽  
Engineering Application ◽  
Tissue Engineering Application ◽  
Medium Chain ◽  
Medium Chain Length ◽  
Study Results

Currently, the selection of materials for tissue engineering scaffolds is still limited because some tissues require flexible and compatible materials with human cells. Medium-chain-length polyhydroxyalkanoate (MCL-PHA) synthesized in microorganisms is an interesting polymer for use in this area and has elastomeric properties compatible with the human body. MCL-PHAs are elastomers with biodegradability and cellular compatibility, making them an attractive material for fabricating soft tissue that requires high elasticity. In this research, MCL-PHA was produced by fed-batch fermentation that Pseudomonas Putida ATCC 47054 was cultured to accumulate MCL-PHA by using glycerol and sodium octanoate as carbon sources. The amounts of dry cell density, MCL-PHA product per dry cells, and MCL-PHA productivity were at 15 g/L, 27%, and 0.067 g/L/h, respectively, and the components of MCL-PHA consisting of 3-hydroxydecanoate (3HD) 64.5%, 3-hydroxyoctanoate (3HO) 32.2%, and 3-hydroxyhexanoate (3HHx) 3.3%. The biosynthesized MCL-PHA terpolyester has a relatively low melting temperature, low crystallinity, and high ductility at 52 °C, 15.7%, and 218%, respectively, and considering as elastomeric polyester. The high-resolution scaffold of MCL-PHA terpolyester biomaterial-ink (approximately 0.36 mm porous size) could be printed in a selected condition with a 3D printer, similar to the optimum pore size for cell attachment and proliferation. The rheological characteristic of this MCL-PHA biomaterial-ink exhibits shear-thinning behavior, leading to good shape fidelity. The study results yielded a condition capable of fabricating an elastomer scaffold of the MCL-PHA terpolyester, giving rise to the ideal soft tissue engineering application.

Unmodified medium chain length polyhydroxyalkanoate (uMCL-PHA) as a thin film for tissue engineering application – characterization and in vitro biocompatibility

2015 ◽  
Vol 141 ◽  
pp. 55-58 ◽  
Author(s):  
Sangeetha Vasudevaraj Naveen ◽  
Irene Kit Ping Tan ◽  
Yuh Shan Goh ◽  
Hanumantha Rao Balaji Raghavendran ◽  
Malliga Raman Murali ◽  
...  
Keyword(s):  
Thin Film ◽  
Tissue Engineering ◽  
Chain Length ◽  
Engineering Application ◽  
Tissue Engineering Application ◽  
Medium Chain ◽  
In Vitro Biocompatibility ◽  
Medium Chain Length

Transformed Cuttlefish Bone Scaffolds for Bone Tissue Engineering

2010 ◽  
Vol 89-91 ◽  
pp. 47-52 ◽  
Author(s):  
Elisa Battistella ◽  
Silvia Mele ◽  
S. Pietronave ◽  
Ismaela Foltran ◽  
G.I. Lesci ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Cell Attachment ◽  
Engineering Application ◽  
Surface Characteristics ◽  
Bone Tissue Regeneration ◽  
Tissue Engineering Application ◽  
Bone Scaffolds ◽  
Surface Development

Nature is full of many interesting things to work with, but many natural resources are also protected. In this view the recycling of aquaculture and fishery residues may lead to the manufacture of new devices and the isolation of new molecules with potential application in medicine. The aim of the present study was to explore the possibility to transform the cuttlefish bone into an hydroxyapatite scaffold suitable for bone tissue engineering application. The mixture of different lamellar porous structure of cuttlefish bone from the species Sepia Officinalis was selected and characterized, according to morphology (including porosity, surface development, surface characteristics) and mechanical properties. The material was transformed into suitable scaffold for bone tissue regeneration, trying to totally or partially convert calcium carbonate (aragonite) into calcium phosphate (hydroxyapatite HA) using hydrothermal transformation. The studies on cell attachment and proliferation (by MTT assay at different experimental times), cell morphology with Scanning Electron Microscopy (SEM), alkaline phosphatase (ALP) and osteocalcin (OC) activities and expressions by mouse osteoblast-like MC3T3-E1 cells on HA were investigated at different experimental times in cultures, in comparison with those observed on titanium specimens used as a control (ET and ST). Cell proliferation was less in HA transformed cuttlefish bone scaffolds than in ET and ST specimens. In contrast, good performance for osteoblasts differentiation was observed on HA transformed cuttlefish bone scaffolds, similar to those observed onto titanium scaffolds.

scholarly journals Biosynthesis of medium chain length poly-3-hydroxyalkanoates by Pseudomonas guezennei from various carbon sources

2008 ◽  
Vol 68 (11) ◽  
pp. 1534-1541 ◽  
Author(s):  
Christelle Simon-Colin ◽  
Gérard Raguénès ◽  
Bernard Costa ◽  
Jean Guezennec
Keyword(s):  
Chain Length ◽  
Carbon Sources ◽  
Medium Chain ◽  
Medium Chain Length

scholarly journals Draft Genome Sequence ofPseudomonas putidaCA-3, a Bacterium Capable of Styrene Degradation and Medium-Chain-Length Polyhydroxyalkanoate Synthesis

2018 ◽  
Vol 6 (4) ◽  
Author(s):  
Eduardo L. Almeida ◽  
Lekha M. Margassery ◽  
Niall O’Leary ◽  
Alan D. W. Dobson
Keyword(s):  
Pseudomonas Putida ◽  
Chain Length ◽  
Genome Sequence ◽  
Draft Genome ◽  
Carbon Sources ◽  
Draft Genome Sequence ◽  
Coding Sequences ◽  
Medium Chain ◽  
Cellular Processes ◽  
Medium Chain Length

ABSTRACTPseudomonas putidastrain CA-3 is an industrial bioreactor isolate capable of synthesizing biodegradable polyhydroxyalkanoate polymers via the metabolism of styrene and other unrelated carbon sources. The pathways involved are subject to regulation by global cellular processes. The draft genome sequence is 6,177,154 bp long and contains 5,608 predicted coding sequences.

scholarly journals Gelatin-Modified Polyurethanes for Soft Tissue Scaffold

2013 ◽  
Vol 2013 ◽  
pp. 1-12 ◽  
Author(s):  
Justyna Kucińska-Lipka ◽  
Iga Gubańska ◽  
Helena Janik
Keyword(s):  
Tissue Engineering ◽  
Soft Tissue ◽  
Positive Impact ◽  
Engineering Application ◽  
Synthetic Polymers ◽  
Hexamethylene Diisocyanate ◽  
Tissue Engineering Application ◽  
Natural Polymers ◽  
Tissue Scaffold ◽  
Pu Foams

Recently, in the field of biomaterials for soft tissue scaffolds, the interest of their modification with natural polymersis growing. Synthetic polymers are often tough, and many of them do not possess fine biocompatibility. On the other hand, natural polymers are biocompatible but weak when used alone. The combination of natural and synthetic polymers gives the suitable properties for tissue engineering requirements. In our study, we modified gelatin synthetic polyurethanes prepared from polyester poly(ethylene-butylene adipate) (PEBA), aliphatic 1,6-hexamethylene diisocyanate (HDI), and two different chain extenders 1,4-butanediol (BDO) or 1-ethoxy-2-(2-hydroxyethoxy)ethanol (EHEE). From a chemical point of view, we replaced expensive components for building PU, such as 2,6-diisocyanato methyl caproate (LDI) and 1,4-diisocyanatobutane (BDI), with cost-effective HDI. The gelatin was added in situ (in the first step of synthesis) to polyurethane to increase biocompatibility and biodegradability of the obtained material. It appeared that the obtained gelatin-modified PU foams, in which chain extender was BDO, had enhanced interactions with media and their hydrolytic degradation profile was also improved for tissue engineering application. Furthermore, the gelatin introduction had positive impact on gelatin-modified PU foams by increasing their hemocompatibility.

scholarly journals Development of a New Strategy for Production of Medium-Chain-Length Polyhydroxyalkanoates by Recombinant Escherichia coli via Inexpensive Non-Fatty Acid Feedstocks

2011 ◽  
Vol 78 (2) ◽  
pp. 519-527 ◽  
Author(s):  
Qin Wang ◽  
Ryan C. Tappel ◽  
Chengjun Zhu ◽  
Christopher T. Nomura
Keyword(s):  
Escherichia Coli ◽  
Fatty Acid ◽  
Chain Length ◽  
Acyl Carrier Protein ◽  
Carbon Sources ◽  
Content Type ◽  
Medium Chain ◽  
Medium Chain Length ◽  
Coa Transferase ◽  
Fatty Acid Carbon

ABSTRACTPseudomonas putidaKT2440 is capable of producing medium-chain-length polyhydroxyalkanoates (MCL-PHAs) when grown on unrelated carbon sources during nutrient limitation. Transcription levels of genes putatively involved in PHA biosynthesis were assessed by quantitative real-time PCR (qRT-PCR) inP. putidagrown on glycerol as a sole carbon source. The results showed that two genes,phaGand the PP0763 gene, were highly upregulated among genes potentially involved in the biosynthesis of MCL-PHAs from unrelated carbon sources. Previous studies have describedphaGas a 3-hydroxyacyl-acyl carrier protein (ACP)-coenzyme A (CoA) transferase, and based on homology, the PP0763 gene was predicted to encode a medium-chain-fatty-acid CoA ligase. High expression levels of these genes during PHA production inP. putidaled to the hypothesis that these two genes are involved in PHA biosynthesis from non-fatty acid carbon sources, such as glucose and glycerol. ThephaGppand PP0763 genes fromP. putidawere cloned and coexpressed with the engineeredPseudomonassp. 61-3 PHA synthase genephaCl(STQK)psin recombinantEscherichia coli. Up to 400 mg liter−1MCL-PHAs was successfully produced from glucose. This study has produced the largest amount of MCL-PHAs reported from non-fatty acid carbon sources in recombinantE. colito date and opens up the possibility of using inexpensive feedstocks to produce MCL-PHA polymers.

scholarly journals Production of a novel medium chain length poly(3‐hydroxyalkanoate) using unprocessed biodiesel waste and its evaluation as a tissue engineering scaffold

2017 ◽  
Vol 10 (6) ◽  
pp. 1384-1399 ◽  
Author(s):  
Pooja Basnett ◽  
Barbara Lukasiewicz ◽  
Elena Marcello ◽  
Harpreet K. Gura ◽  
Jonathan C. Knowles ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Chain Length ◽  
Tissue Engineering Scaffold ◽  
Medium Chain ◽  
Medium Chain Length
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