scholarly journals Native Spider Silk-Based Antimicrobial Hydrogels for Biomedical Applications

Polymers ◽  
2021 ◽  
Vol 13 (11) ◽  
pp. 1796
Author(s):  
Sinith Withanage ◽  
Artemii Savin ◽  
Valeria Nikolaeva ◽  
Aleksandra Kiseleva ◽  
Marina Dukhinova ◽  
...  
Keyword(s):  
Hyaluronic Acid ◽  
3D Printing ◽  
Spider Silk ◽  
Physicochemical Characterization ◽  
Electron Microscopic ◽  
Antimicrobial Drug Resistance ◽  
Hybrid Hydrogels ◽  
Surgical Meshes ◽  
Ability Tests ◽  
Enzymatic Degradability

Novel antimicrobial natural polymeric hybrid hydrogels based on hyaluronic acid (HA) and spider silk (Ss) were prepared using the chemical crosslinking method. The effects of the component ratios on the hydrogel characteristics were observed parallel to the primary physicochemical characterization of the hydrogels with scanning electron microscopic imaging, Fourier-transform infrared spectroscopy, and contact angle measurements, which confirmed the successful crosslinking, regular porous structure, exact composition, and hydrophilic properties of hyaluronic acid/spider silk-based hydrogels. Further characterizations of the hydrogels were performed with the swelling degree, enzymatic degradability, viscosity, conductivity, and shrinking ability tests. The hyaluronic acid/spider silk-based hydrogels do not show drastic cytotoxicity over human postnatal fibroblasts (HPF). Hydrogels show extraordinary antimicrobial ability on both gram-negative and gram-positive bacteria. These hydrogels could be an excellent alternative that aids in overcoming antimicrobial drug resistance, which is considered to be one of the major global problems in the biomedical industry. Hyaluronic acid/spider silk-based hydrogels are a promising material for collaborated antimicrobial and anti-inflammatory drug delivery systems for external use. The rheological properties of the hydrogels show shear-thinning properties, which suggest that the hydrogels could be applied in 3D printing, such as in the 3D printing of antimicrobial surgical meshes.

Physicochemical characterization and in vitro antioxidant activity of hyaluronic acid produced by Streptococcus zooepidemicus CCT 7546

2021 ◽  
pp. 1-10
Author(s):  
Wilza Kímilly Vital de Paiva ◽  
Waleska Rayane Dantas Bezerra de Medeiros ◽  
Cristiane Fernandes de Assis ◽  
Everaldo Silvino dos Santos ◽  
Francisco Caninde de Sousa Júnior
Keyword(s):  
Antioxidant Activity ◽  
Hyaluronic Acid ◽  
Physicochemical Characterization ◽  
Streptococcus Zooepidemicus ◽  
In Vitro Antioxidant Activity

Gelatin–alginate–hyaluronic acid inks for 3D printing: effects of bioglass addition on printability, rheology and scaffold tensile modulus

2021 ◽  
Author(s):  
Marcos Bertuola ◽  
Beatriz Aráoz ◽  
Ulises Gilabert ◽  
Ana Gonzalez-Wusener ◽  
Mercedes Pérez-Recalde ◽  
...  
Keyword(s):  
Hyaluronic Acid ◽  
3D Printing ◽  
Tensile Modulus

scholarly journals 3D Printing of Drug Nanocrystals for Film Formulations

Molecules ◽  
2021 ◽  
Vol 26 (13) ◽  
pp. 3941
Author(s):  
Giorgia Germini ◽  
Leena Peltonen
Keyword(s):  
Particle Size ◽  
3D Printing ◽  
Polymer Films ◽  
Film Studies ◽  
Polymer Concentration ◽  
Physicochemical Characterization ◽  
Methyl Cellulose ◽  
Polymeric Film ◽  
Content Uniformity ◽  
3D Printed

The aim of the study was to prepare indomethacin nanocrystal-loaded, 3D-printed, fast-dissolving oral polymeric film formulations. Nanocrystals were produced by the wet pearl milling technique, and 3D printing was performed by the semi-solid extrusion method. Hydroxypropyl methyl cellulose (HPMC) was the film-forming polymer, and glycerol the plasticizer. In-depth physicochemical characterization was made, including solid-state determination, particle size and size deviation analysis, film appearance evaluation, determination of weight variation, thickness, folding endurance, drug content uniformity, and disintegration time, and drug release testing. In drug nanocrystal studies, three different stabilizers were tested. Poloxamer F68 produced the smallest and most homogeneous particles, with particle size values of 230 nm and PI values below 0.20, and was selected as a stabilizer for the drug-loaded film studies. In printing studies, the polymer concentration was first optimized with drug-free formulations. The best mechanical film properties were achieved for the films with HPMC concentrations of 2.85% (w/w) and 3.5% (w/w), and these two HPMC levels were selected for further drug-loaded film studies. Besides, in the drug-loaded film printing studies, three different drug levels were tested. With the optimum concentration, films were flexible and homogeneous, disintegrated in 1 to 2.5 min, and released the drug in 2–3 min. Drug nanocrystals remained in the nano size range in the polymer films, particle sizes being in all film formulations from 300 to 500 nm. When the 3D-printed polymer films were compared to traditional film-casted polymer films, the physicochemical behavior and pharmaceutical performance of the films were very similar. As a conclusion, 3D printing of drug nanocrystals in oral polymeric film formulations is a very promising option for the production of immediate-release improved- solubility formulations.

Glycosaminoglycan biosynthesis in the isolated perfused rat lung

1984 ◽  
Vol 57 (6) ◽  
pp. 1648-1654 ◽  
Author(s):  
P. M. Sampson ◽  
R. B. Boyd ◽  
G. G. Pietra ◽  
A. P. Fishman
Keyword(s):  
Hyaluronic Acid ◽  
Heparan Sulfate ◽  
Dermatan Sulfate ◽  
Rat Lung ◽  
Electron Microscopic ◽  
Chondroitin Sulfates ◽  
Isolated Lung ◽  
Microscopic Studies

The suitability of an isolated lung, perfused under carefully monitored conditions, for the study of the biosynthesis of glycosaminoglycans was examined for the rat lung using either [35S]-sulfate or [6-3H]glucosamine. Metabolic and electron-microscopic studies after 3 h of perfusion showed that under the conditions of this study the isolated lung showed no anatomical or metabolic derangements. All glycosaminoglycans normally synthesized in the intact lung were identified. The predominant glycosaminoglycan was heparan sulfate (40% of total). Approximately 14% of the glucosamine incorporated into the glycosaminoglycans was found in hyaluronic acid. Less than 5% of either label was in heparin. The remainder of the synthesized glycosaminoglycans, with the exception of 10% which could not be identified, consisted of the chondroitin sulfates and dermatan sulfate. The relative proportions of the newly synthesized glycosaminoglycans, including the low amounts of heparin, are similar to those found in isolation of endogenous lung glycosaminoglycans. The isolated perfused rat lung appears to be a useful model for the study of glycosaminoglycan biosynthesis by the intact lung.

Optical stereolithography of antifouling zwitterionic hydrogels

2019 ◽  
Vol 7 (17) ◽  
pp. 2855-2864 ◽  
Author(s):  
Wenyang Pan ◽  
Thomas J. Wallin ◽  
Jérémy Odent ◽  
Mighten C. Yip ◽  
Bobak Mosadegh ◽  
...  
Keyword(s):  
3D Printing ◽  
Smart Materials ◽  
Sensor Applications ◽  
Hybrid Hydrogels

This paper reports the rapid 3D printing of tough (toughness, UT, up to 141.6 kJ m−3), highly solvated (ϕwater ∼ 60 v/o), and antifouling hybrid hydrogels for potential uses in biomedical, smart materials, and sensor applications, using a zwitterionic photochemistry compatible with stereolithography (SLA).

Arginine derivatives assist dopamine-hyaluronic acid hybrid hydrogels to have enhanced antioxidant activity for wound healing

2020 ◽  
Vol 392 ◽  
pp. 123775 ◽  
Author(s):  
Shaohan Zhang ◽  
Jingyi Hou ◽  
Qijuan Yuan ◽  
Peikun Xin ◽  
Huitong Cheng ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Wound Healing ◽  
Hyaluronic Acid ◽  
Hybrid Hydrogels

Silver Sulphadiazine- xanthan gum- hyaluronic Acid Composite Hydrogel for Wound Healing: Formulation Development and in vivo Evaluation

2020 ◽  
Vol 16 (1) ◽  
pp. 21-29 ◽  
Author(s):  
M.O. Ilomuanya ◽  
Z.A. Seriki ◽  
U.N. Ubani-Ukoma ◽  
B.A. Oseni ◽  
B.O. Silva
Keyword(s):  
Wound Healing ◽  
Hyaluronic Acid ◽  
Xanthan Gum ◽  
Wound Closure ◽  
Antimicrobial Agents ◽  
Biological Properties ◽  
Formulation Development ◽  
Hybrid Hydrogels ◽  
Silver Sulphadiazine

Background: Development and modifications of hybrid hydrogels have been done to improve biological properties or to decrease the disadvantages of biomaterials.Objectives: The efficacy of hyaluronic acid in combination with silver sulphadiazine in wound healing was investigated. The retaining properties of xanthan gum to aid re- epithelialization was also explored.Materials and Method: Four hybrid hydrogels comprising of different concentrations of xanthan gum, eugenol and antimicrobial agents – hyaluronic acid and silver sulphadiazine were formulated. The physicochemical properties of the gels were assessed, and the antimicrobial effectiveness of the different hydrogel were determined using the extent of wound closure as an index.Results: The hydrogel samples had approximately 90% moisture content with rate of evaporation between 26- 32% for a 5 h period at 37oC. The pH of all formulations was between 7.59 - 8.05 considering that the formulation would be applied to underlying tissues of the skin. The swelling index after a 12 h period in distilled water was 10% for HX 1, 27% for HX 2, 29% for HX 3 and 30% for HX 4. There was no new peak observed in the FTIR analysis to indicate formation of new bonds.Conclusion: Incorporation of silver sulphadiazine at 0.1% and hyaluronic acid at 1.5% in the formulation yielded the best results with regards to least presence of inflammatory cell infiltrates and excellent wound closure at 14 days compared to the control and other formulations. Further investigation may be required for clinical use as an effective wound dressing material. Keywords: Silver sulphadiazine, Xanthan gum, Hyaluronic acid, Hydrogels, Wound healing.

Double printing of hyaluronic acid/poly(glycidol) hybrid hydrogels with poly( ε -caprolactone) for MSC chondrogenesis

2017 ◽  
Vol 9 (4) ◽  
pp. 044108 ◽  
Author(s):  
Simone Stichler ◽  
Thomas Böck ◽  
Naomi Paxton ◽  
Sarah Bertlein ◽  
Riccardo Levato ◽  
...  
Keyword(s):  
Hyaluronic Acid ◽  
Hybrid Hydrogels

scholarly journals Mechanically Enhanced Hyaluronic Acid Hybrid Hydrogels with Halloysite Nanotubes

2017 ◽  
Vol 46 (8) ◽  
pp. 1217-1219 ◽  
Author(s):  
Kyung-Lynne Park ◽  
Wei Ma ◽  
Yuji Higaki ◽  
Atsushi Takahara
Keyword(s):  
Hyaluronic Acid ◽  
Halloysite Nanotubes ◽  
Hybrid Hydrogels
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