scholarly journals Poly(ethylene-imine)-Functionalized Magnetite Nanoparticles Derivatized with Folic Acid: Heating and Targeting Properties

Polymers ◽  
2021 ◽  
Vol 13 (10) ◽  
pp. 1599
Author(s):  
Mariano Ortega-Muñoz ◽  
Simona Plesselova ◽  
Angel V. Delgado ◽  
Francisco Santoyo-Gonzalez ◽  
Rafael Salto-Gonzalez ◽  
...  
Keyword(s):  
Magnetic Field ◽  
Folic Acid ◽  
Cell Viability ◽  
Magnetite Nanoparticles ◽  
High Expression ◽  
Ethylene Imine ◽  
One Pot ◽  
Molecular Weights ◽  
Poly Ethylene

Magnetite nanoparticles (MNPs) coated by branched poly (ethylene-imine) (PEI) were synthesized in a one-pot. Three molecular weights of PEI were tested, namely, 1.8 kDa (sample MNP-1), 10 kDa (sample MNP-2), and 25 kDa (sample MNP-3). The MNP-1 particles were further functionalized with folic acid (FA) (sample MNP-4). The four types of particles were found to behave magnetically as superparamagnetic, with MNP-1 showing the highest magnetization saturation. The particles were evaluated as possible hyperthermia agents by subjecting them to magnetic fields of 12 kA/m strength and frequencies ranging between 115 and 175 kHz. MNP-1 released the maximum heating power, reaching 330 W/g at the highest frequency, in the high side of reported values for spherical MNPs. In vitro cell viability assays of MNP-1 and MNP-4 against three cell lines expressing different levels of FA receptors (FR), namely, HEK (low expression), and HeLa (high expression), and HepG2 (high expression), demonstrated that they are not cytotoxic. When the cells were incubated in the presence of a 175 kHz magnetic field, a significant reduction in cell viability and clone formation was obtained for the high expressing FR cells incubated with MNP-4, suggesting that MNP-4 particles are good candidates for magnetic field hyperthermia and active targeting.

Green Wood Adhesives from One-Pot Coacervation of Folic Acid and Branched Poly(ethylene imine)

2021 ◽  
Vol 4 (9) ◽  
pp. 7314-7321
Author(s):  
Shuitao Gao ◽  
Jinwan Qi ◽  
Shasha Jiang ◽  
Tongyue Wu ◽  
Wenkai Wang ◽  
...  
Keyword(s):  
Folic Acid ◽  
Ethylene Imine ◽  
One Pot ◽  
Wood Adhesives ◽  
Green Wood ◽  
Poly Ethylene

scholarly journals Comparison of Linear Poly Ethylene Imine (LPEI) and Poly L-Lysine (PLL) in Fabrication of CHOK1 Cell-Loaded Multilayer Alginate Microcapsules

2020 ◽  
Vol 10 (2) ◽  
pp. 290-296
Author(s):  
Fariba Hajifathaliha ◽  
Arash Mahboubi ◽  
Elham Mohit ◽  
Noushin Bolourchian ◽  
Vahid Khalaj ◽  
...  
Keyword(s):  
Cell Viability ◽  
Cell Survival ◽  
Metabolic Activity ◽  
Encapsulation Efficiency ◽  
Cell Encapsulation ◽  
High Price ◽  
Ethylene Imine ◽  
Linear Poly ◽  
Cellular Metabolic Activity ◽  
Poly Ethylene

Purpose: Poly l-lysine (PLL) has been introduced as a strengthening covering layer for alginate microcapsules which are the most convenient way for cell encapsulation. Some disadvantages of PLL such as high price and low biocompatibility have prompted scientists to find better alternatives. Linear poly ethylene imine (LPEI), thanks to its highly similar structure to PLL, could be considered as a proper cost-effective alternative. In this study LPEI and PLL were compared as covering layers of cell-loaded alginate-LPEI-alginate (cALA) and alginate-PLL-alginate (cAPA) microcapsules. Methods: In addition to the physico-mechanical properties, the encapsulation efficiency, cell survival post encapsulation, cell viability, and cellular metabolic activity within the microcapsules were evaluated using trypan blue, live/dead cell staining, and MTT test, respectively. Results: Physico-mechanical evaluation of the microcapsules revealed that the cell microencapsulation process did not affect their shape, size, and mechanical stability. Although the encapsulation efficiency for cALA and cAPA was not different (P>0.05), cell survival post encapsulation was higher in cALA than in cAPA (P<0.05) which could be the reason for the higher cell viability and also cellular metabolic activity within these microcapsules in comparison to cAPA. Conclusion: Here, based on these results, ALA could be introduced as a preferable alternative to APA for cell encapsulation.

scholarly journals Cyclopropenium Nanoparticles and Gene Transfection in Cells

Pharmaceutics ◽  
2020 ◽  
Vol 12 (8) ◽  
pp. 768
Author(s):  
Noam Y. Steinman ◽  
Luis M. Campos ◽  
Yakai Feng ◽  
Abraham J. Domb ◽  
Hossein Hosseinkhani
Keyword(s):  
Viral Vectors ◽  
Gene Transfection ◽  
Medical Science ◽  
Genetic Material ◽  
Mouse Fibroblast ◽  
Ethylene Imine ◽  
Efficient Gene ◽  
Poly Ethylene ◽  
First Time

Non-viral vectors for the transfection of genetic material are at the frontier of medical science. In this article, we introduce for the first time, cyclopropenium-containing nanoparticles as a cationic carrier for gene transfection, as an alternative to the common quaternary ammonium transfection agents. Cyclopropenium-based cationic nanoparticles were prepared by crosslinking poly(ethylene imine) (PEI) with tetrachlorocyclopropene. These nanoparticles were electrostatically complexed with plasmid DNA into nanoparticles (~50 nm). Their cellular uptake into F929 mouse fibroblast cells, and their eventual expression in vitro have been described. Transfection is enhanced relative to PEI with minimal toxicity. These cyclopropenium nanoparticles possess efficient gene transfection capabilities with minimal cytotoxicity, which makes them novel and promising candidates for gene therapy.

Poly(ethylene imine) Nanocarriers Do Not Induce Mutations nor Oxidative DNA Damage in Vitro in MutaMouse FE1 Cells

2011 ◽  
Vol 8 (3) ◽  
pp. 976-981 ◽  
Author(s):  
Andrea Beyerle ◽  
Alexandra S. Long ◽  
Paul A. White ◽  
Thomas Kissel ◽  
Tobias Stoeger
Keyword(s):  
Dna Damage ◽  
Oxidative Dna Damage ◽  
Ethylene Imine ◽  
Poly Ethylene

scholarly journals Paclitaxel-loaded Folate-targeted Albumin-Alginate Nanoparticles Crosslinked with Ethylenediamine. Synthesis and In Vitro Characterization.

2021 ◽  
Author(s):  
A.M. Martínez ◽  
M. Benito ◽  
E. Pérez ◽  
C. Teijón ◽  
R.M. Olmo ◽  
...  
Keyword(s):  
Folic Acid ◽  
Cell Membrane ◽  
Cell Viability ◽  
Folate Receptor ◽  
Antineoplastic Drugs ◽  
Secondary Effects ◽  
In Vitro Characterization ◽  
Nanometric Range ◽  
Mcf 7

Among the different ways to reduce the secondary effects of antineoplastic drugs in cancer treatment, the use of nanoparticles has demonstrated good results due to the protection of the drug and the possibility of releasing compounds to a specific therapeutic target. The &alpha;-isoform of folate receptor (FR) is overexpressed on a significant number of human cancers; therefore, folate-targeted crosslinked nanoparticles based on BSA and alginate mixtures and loaded with paclitaxel (PTX) have been prepared to maximizing the proven antineoplastic activity of the drug against solid tumors. Nanometric-range sized particles (169 &plusmn; 28nm - 296 &plusmn; 57nm), with negative Z-potential values (between -0.12 &plusmn; 0.04 and -94.1&plusmn; 0.4), were synthesized. The loaded PTX (2.63&plusmn;0.19 - 3.56 &plusmn;0.13 &micro;g PTX/mg Np) was sustainably released along 23 and 27h. Three cell lines (MCF-7, MDA-MB-231 and HeLa) were selected to test the efficacy of the folate-targeted PTX-loaded BSA/ALG nanocarriers. The presence of FR on cell membrane led to a significant larger uptake of BSA/ALG-Fol nanoparticles regarding to the equivalent nanoparticles without folic acid on its surface. The cell viability results demonstrated a cytocompatibility of unloaded nanoparticle-Fol and a gradual decrease in cell viability after treatment with PTX-loaded nanoparticles-Fol due to the sustainable PTX release.

PEGylated poly(ethylene imine) copolymer-delivered siRNA inhibits HIV replication in vitro

2012 ◽  
Vol 157 (1) ◽  
pp. 55-63 ◽  
Author(s):  
Nick D. Weber ◽  
Olivia M. Merkel ◽  
Thomas Kissel ◽  
María Ángeles Muñoz-Fernández
Keyword(s):  
Hiv Replication ◽  
Ethylene Imine ◽  
Poly Ethylene
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