PEGylated poly(ethylene imine) copolymer-delivered siRNA inhibits HIV replication in vitro

2012 ◽  
Vol 157 (1) ◽  
pp. 55-63 ◽  
Author(s):  
Nick D. Weber ◽  
Olivia M. Merkel ◽  
Thomas Kissel ◽  
María Ángeles Muñoz-Fernández
Keyword(s):  
Hiv Replication ◽  
Ethylene Imine ◽  
Poly Ethylene

scholarly journals Cyclopropenium Nanoparticles and Gene Transfection in Cells

Pharmaceutics ◽  
2020 ◽  
Vol 12 (8) ◽  
pp. 768
Author(s):  
Noam Y. Steinman ◽  
Luis M. Campos ◽  
Yakai Feng ◽  
Abraham J. Domb ◽  
Hossein Hosseinkhani
Keyword(s):  
Viral Vectors ◽  
Gene Transfection ◽  
Medical Science ◽  
Genetic Material ◽  
Mouse Fibroblast ◽  
Ethylene Imine ◽  
Efficient Gene ◽  
Poly Ethylene ◽  
First Time

Non-viral vectors for the transfection of genetic material are at the frontier of medical science. In this article, we introduce for the first time, cyclopropenium-containing nanoparticles as a cationic carrier for gene transfection, as an alternative to the common quaternary ammonium transfection agents. Cyclopropenium-based cationic nanoparticles were prepared by crosslinking poly(ethylene imine) (PEI) with tetrachlorocyclopropene. These nanoparticles were electrostatically complexed with plasmid DNA into nanoparticles (~50 nm). Their cellular uptake into F929 mouse fibroblast cells, and their eventual expression in vitro have been described. Transfection is enhanced relative to PEI with minimal toxicity. These cyclopropenium nanoparticles possess efficient gene transfection capabilities with minimal cytotoxicity, which makes them novel and promising candidates for gene therapy.

Poly(ethylene imine) Nanocarriers Do Not Induce Mutations nor Oxidative DNA Damage in Vitro in MutaMouse FE1 Cells

2011 ◽  
Vol 8 (3) ◽  
pp. 976-981 ◽  
Author(s):  
Andrea Beyerle ◽  
Alexandra S. Long ◽  
Paul A. White ◽  
Thomas Kissel ◽  
Tobias Stoeger
Keyword(s):  
Dna Damage ◽  
Oxidative Dna Damage ◽  
Ethylene Imine ◽  
Poly Ethylene

Ni(II)-NTA Modified Poly(ethylene imine) Glycopolymers: Physicochemical Properties and First In Vitro Study of Polyplexes Formed with HIV-Derived Peptides

2013 ◽  
Vol 13 (5) ◽  
pp. 531-538 ◽  
Author(s):  
Nicole Hauptmann ◽  
Marjorie Pion ◽  
María-Ángeles Muñoz-Fernández ◽  
Hartmut Komber ◽  
Carsten Werner ◽  
...  
Keyword(s):  
Physicochemical Properties ◽  
In Vitro Study ◽  
Vitro Study ◽  
Ethylene Imine ◽  
Poly Ethylene

Crosslinked nanocarriers based upon poly(ethylene imine) for systemic plasmid delivery: In vitro characterization and in vivo studies in mice

2007 ◽  
Vol 118 (3) ◽  
pp. 370-380 ◽  
Author(s):  
Michael Neu ◽  
Oliver Germershaus ◽  
Shirui Mao ◽  
Karl-Heinz Voigt ◽  
Martin Behe ◽  
...  
Keyword(s):  
In Vivo Studies ◽  
Ethylene Imine ◽  
In Vitro Characterization ◽  
Poly Ethylene ◽  
Plasmid Delivery

scholarly journals Nanocarriers for Protein Delivery to the Cytosol: Assessing the Endosomal Escape of Poly(Lactide-co-Glycolide)-Poly(Ethylene Imine) Nanoparticles

Nanomaterials ◽  
2019 ◽  
Vol 9 (4) ◽  
pp. 652 ◽  
Author(s):  
Marianna Galliani ◽  
Chiara Tremolanti ◽  
Giovanni Signore
Keyword(s):  
Protein Delivery ◽  
Therapeutic Proteins ◽  
Endosomal Escape ◽  
Ethylene Imine ◽  
Enzyme Superoxide Dismutase ◽  
Rapid Clearance ◽  
Poly Ethylene ◽  
Enzyme Delivery

Therapeutic proteins and enzymes are a group of interesting candidates for the treatment of numerous diseases, but they often require a carrier to avoid degradation and rapid clearance in vivo. To this end, organic nanoparticles (NPs) represent an excellent choice due to their biocompatibility, and cross-linked enzyme aggregates (CLEAs)-loaded poly (lactide-co-glycolide) (PLGA) NPs have recently attracted attention as versatile tools for targeted enzyme delivery. However, PLGA NPs are taken up by cells via endocytosis and are typically trafficked into lysosomes, while many therapeutic proteins and enzymes should reach the cellular cytosol to perform their activity. Here, we designed a CLEAs-based system implemented with a cationic endosomal escape agent (poly(ethylene imine), PEI) to extend the use of CLEA NPs also to cytosolic enzymes. We demonstrated that our system can deliver protein payloads at cytoplasm level by two different mechanisms: Endosomal escape and direct translocation. Finally, we applied this system to the cytoplasmic delivery of a therapeutically relevant enzyme (superoxide dismutase, SOD) in vitro.

scholarly journals Stabilized Amorphous Calcium Carbonate as a Precursor of Microcoating on Calcite

Materials ◽  
2020 ◽  
Vol 13 (17) ◽  
pp. 3762
Author(s):  
Taeyoung Jeon ◽  
Ye-Eun Na ◽  
Dongchan Jang ◽  
Il Won Kim
Keyword(s):  
Calcium Carbonate ◽  
Crystal Morphology ◽  
In Vitro Assay ◽  
Single Crystal Substrate ◽  
Amorphous Calcium Carbonate ◽  
Ethylene Imine ◽  
Vitro Assay ◽  
Crystal Substrate ◽  
Poly Ethylene

Highly controlled biomineralization of calcium carbonate is via non-classical mesocrystallization of amorphous precursors. In the present study, a simple in vitro assay was developed to mimic the biological process, which involved stabilized amorphous calcium carbonate and a single crystal substrate of calcite. The microcoating layer formed on the calcite substrate displayed mesocrystalline characteristics, and the layers near the substrate were strongly influenced by the epitaxy to the substrate. This behavior was preserved even when the morphology of the coating layer was modified with poly(acrylic acid), a model anionic macromolecule. Interestingly, the extent of the epitaxy increased substantially with poly(ethylene imine), which barely affected the crystal morphology. The in vitro assay in the present study will be useful in the investigations of the biomineralization and bioinspired crystallization of calcium carbonate in general.

scholarly journals Poly(ethylene-imine)-Functionalized Magnetite Nanoparticles Derivatized with Folic Acid: Heating and Targeting Properties

Polymers ◽  
2021 ◽  
Vol 13 (10) ◽  
pp. 1599
Author(s):  
Mariano Ortega-Muñoz ◽  
Simona Plesselova ◽  
Angel V. Delgado ◽  
Francisco Santoyo-Gonzalez ◽  
Rafael Salto-Gonzalez ◽  
...  
Keyword(s):  
Magnetic Field ◽  
Folic Acid ◽  
Cell Viability ◽  
Magnetite Nanoparticles ◽  
High Expression ◽  
Ethylene Imine ◽  
One Pot ◽  
Molecular Weights ◽  
Poly Ethylene

Magnetite nanoparticles (MNPs) coated by branched poly (ethylene-imine) (PEI) were synthesized in a one-pot. Three molecular weights of PEI were tested, namely, 1.8 kDa (sample MNP-1), 10 kDa (sample MNP-2), and 25 kDa (sample MNP-3). The MNP-1 particles were further functionalized with folic acid (FA) (sample MNP-4). The four types of particles were found to behave magnetically as superparamagnetic, with MNP-1 showing the highest magnetization saturation. The particles were evaluated as possible hyperthermia agents by subjecting them to magnetic fields of 12 kA/m strength and frequencies ranging between 115 and 175 kHz. MNP-1 released the maximum heating power, reaching 330 W/g at the highest frequency, in the high side of reported values for spherical MNPs. In vitro cell viability assays of MNP-1 and MNP-4 against three cell lines expressing different levels of FA receptors (FR), namely, HEK (low expression), and HeLa (high expression), and HepG2 (high expression), demonstrated that they are not cytotoxic. When the cells were incubated in the presence of a 175 kHz magnetic field, a significant reduction in cell viability and clone formation was obtained for the high expressing FR cells incubated with MNP-4, suggesting that MNP-4 particles are good candidates for magnetic field hyperthermia and active targeting.

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