scholarly journals Oxygen Plasma Treated-Electrospun Polyhydroxyalkanoate Scaffolds for Hydrophilicity Improvement and Cell Adhesion

Polymers ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 1056 ◽  
Author(s):  
Asiyah Esmail ◽  
João R. Pereira ◽  
Patrícia Zoio ◽  
Sara Silvestre ◽  
Ugur Deneb Menda ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Oxygen Plasma ◽  
Electrospinning Process ◽  
Dermal Fibroblasts ◽  
Solution Flow Rate ◽  
Complete Wetting ◽  
Solution Flow ◽  
Medium Chain Length ◽  
A Cell ◽  
Copolymer Poly

Poly(hydroxyalkanoates) (PHAs) with differing material properties, namely, the homopolymer poly(3-hydroxybutyrate), P(3HB), the copolymer poly(3-hydroxybutyrate-co-3-hydroxyvalerate), P(3HB-co-3HV), with a 3HV content of 25 wt.% and a medium chain length PHA, and mcl-PHA, mainly composed of 3-hydroxydecanoate, were studied as scaffolding material for cell culture. P(3HB) and P(3HB-co-3HV) were individually spun into fibers, as well as blends of the mcl-PHA with each of the scl-PHAs. An overall biopolymer concentration of 4 wt.% was used to prepare the electrospinning solutions, using chloroform as the solvent. A stable electrospinning process and good quality fibers were obtained for a solution flow rate of 0.5 mL h−1, a needle tip collector distance of 20 cm and a voltage of 12 kV for P(3HB) and P(3HB-co-3HV) solutions, while for the mcl-PHA the distance was increased to 25 cm and the voltage to 15 kV. The scaffolds’ hydrophilicity was significantly increased under exposure to oxygen plasma as a surface treatment. Complete wetting was obtained for the oxygen plasma treated scaffolds and the water uptake degree increased in all treated scaffolds. The biopolymers crystallinity was not affected by the electrospinning process, while their treatment with oxygen plasma decreased their crystalline fraction. Human dermal fibroblasts were able to adhere and proliferate within the electrospun PHA-based scaffolds. The P(3HB-co-3HV): mcl-PHA oxygen plasma treated scaffold highlighted the most promising results with a cell adhesion rate of 40 ± 8%, compared to 14 ± 4% for the commercial oxygen plasma treated polystyrene scaffold AlvetexTM. Scaffolds based on P(3HB-co-3HV): mcl-PHA blends produced by electrospinning and submitted to oxygen plasma exposure are therefore promising biomaterials for the development of scaffolds for tissue engineering.

scholarly journals Removal of isopropyl alcohol by electrical discharge from an aqueous solution with microbubbles

2021 ◽  
pp. 32-37
Author(s):  
Vladislav Panov ◽  
Vladimir Pecherkin ◽  
Leonid Vasilyak ◽  
Yurii Kulikov ◽  
Sergei Vetchinin ◽  
...  
Keyword(s):  
Water Flow ◽  
Electric Discharge ◽  
Isopropyl Alcohol ◽  
Electrode System ◽  
Solution Flow Rate ◽  
Air Bubbles ◽  
Solution Flow ◽  
A Cell ◽  
Discharge Cell ◽  
Industrial Frequency

The removal of isopropyl alcohol impurities with an initial volume concentration of 20 % in a cell with a working area volume of 831 cm 3 in a water flow with fine air bubbles with a solution flow rate of 2 m 3/h by a quasi-volume electric discharge obtained using a multi-electrode system of sectioned needle electrodes has been experimentally investigated. At an alternating voltage of an industrial frequency of 50 Hz, the creation of a finely dispersed phase with air bubbles in an electric discharge cell increases the efficiency of isopropyl alcohol removal from the water flow by 6 %.

Electrospinning of PAN and composite PAN-GO nanofibres

2018 ◽  
Vol 1 (91) ◽  
pp. 18-26 ◽  
Author(s):  
W. Matysiak ◽  
T. Tański ◽  
W. Smok
Keyword(s):  
Graphene Oxide ◽  
High Specific Surface Area ◽  
Electrospinning Process ◽  
Structural Defects ◽  
Solution Flow Rate ◽  
Solution Flow ◽  
X Ray ◽  
Electrospinning Method ◽  
Morphology And Structure ◽  
Made In

Purpose: The aim of this study was to present the influence of used reinforcement phase – graphene oxide (GO) and the electrospinning process parameters (the distance between the nozzle and collector) on the morphology and the structure of the obtained composite PAN-GO nanofibres. Design/methodology/approach: To produce pure polymer nanofibers, a 10% (wt.) electrospinning solution the polyacrylonitrile (PAN) was dissolved in N, N-dimethylformamide (DMF). The spinning solution used for electrospinning PAN-GO composite fibres was made by dissolving the PAN in a mixture of GO and DMF. By changing the configuration of the distance between the nozzle and collector (10 and 20 cm) and maintaining the remaining parameters (solution flow rate and potential difference between the electrodes), four samples in the form of nanofibrous mats were made. In order to identify the structure and morphology of the reinforcing phase, X-ray microanalysis (EDX) and scanning electron microscopy (SEM) were performed. In addition, the structure of graphene oxide microparticles was investigated by a Raman spectrometer. In order to determine the influence of the distance between the nozzle and the collector used in the electrospinning process and the addition of the reinforcing phase to the morphology and structure of the obtained PAN polymer nanofibres and PAN-GO composite nanofibres, they were examined using SEM. The analysis of the chemical composition of PAN and PAN-GO fibres was carried out using X-ray microanalysis. Findings: The morphology and structure analysis indicated that polymer nanofibres PAN for both the distances between the nozzle and the collector show no structural defects and presented same diameter over the entire length of the fibre. Nanofibres with the addition of GO obtained at both distances between the electrodes, showed defects in the form of beads. In addition, it was observed that with increasing distance between the nozzle and collector the diameter of obtained nanofibres is smaller for both pure PAN and composite PAN-GO samples. Research limitations/implications: The paper is the basis for further research in the field of the use of PAN-GO composite nanofibres as water purification materials. Originality/value: The electrospinning method can be an alternative to conventional methods for the production of filtering membranes due to the ease of carrying out the process and the fact that a material with a high specific surface area is obtained.

scholarly journals Quantitatively Controlled Fabrication of Uniaxially Aligned Nanofibrous Scaffold for Cell Adhesion

2011 ◽  
Vol 2011 ◽  
pp. 1-9 ◽  
Author(s):  
Suk Hee Park ◽  
Jung Woo Hong ◽  
Jennifer Hyunjong Shin ◽  
Dong-Yol Yang
Keyword(s):  
Electrospinning Process ◽  
Dermal Fibroblasts ◽  
Fiber Formation ◽  
Actin Stress Fiber ◽  
Promote Cell Proliferation ◽  
Nanofiber Scaffold ◽  
Cell Proliferation And Differentiation ◽  
Fiber Spacing ◽  
Proliferation And Differentiation ◽  
A Cell

In light of tissue engineering, development of a functional and controllable scaffold which can promote cell proliferation and differentiation is crucial. In this study, we introduce a controllable collection method of the electrospinning process for regularly-distributed and uniaxially oriented nanofiber scaffold and evaluate the effects of aligned nanofiber density on adhesion of dermal fibroblasts. The suggested spinning collector features an inclined void gap, which allows easy transfer of uniformly aligned fibers onto other surfaces. By undergoing multiple transfers, the density of the nanofibers can be quantitatively controlled. The resultant polycaprolactone (PCL) nanofibers had well-defined nanotopography in a 400–600 nm range. Human dermal fibroblasts were seeded on aligned nanofiber scaffolds of different densities achieved by varying the number of transfers. Cell morphology and actin stress fiber formation was accessed after seven days. The experimental results indicate that the contact guidance of the cells along the fiber alignment can be more activated with more than one guidance feature on a cell; that is, the high density of fiber is attained in so much that fiber spacing gets below the cell size.

A REVIEW OF ASSEMBLED POLYACRYLONITRILE-BASED CARBON NANOFIBER PREPARED ELECTROSPINNING PROCESS

2011 ◽  
Vol 10 (03) ◽  
pp. 455-469 ◽  
Author(s):  
A. MATARAM ◽  
A. F. ISMAIL ◽  
M. S. Abdullah ◽  
B. C. Ng ◽  
T. MATSUURA
Keyword(s):  
Polymer Solution ◽  
Flow Rate ◽  
Carbon Nanofiber ◽  
Electrical Potential ◽  
Polymer Melt ◽  
Processing Parameters ◽  
Electrospinning Process ◽  
Solution Flow Rate ◽  
Solution Flow ◽  
Wide Range

Electrospinning is a very simple and versatile process by which polymer nanofibers with diameters ranging from a few nanometers to several micrometers can be produced using an electrostatically driven jet of polymer solution (or polymer melt). Significant progress has been made in this process throughout the last decade and the resultant nanostructures have been exploited to a wide range of applications. An important feature of the electrospinning process is that electrospinning nanofibers are produced in atmospheric air and at room temperature. This paper reviews the assembled polyacrylonitrile (PAN)-based carbon nanofibers with various processing parameters such as electrical potential, distance between capillary and collector drum, solution flow rate (dope extrusion rate), and concentration of polymer solution. The average fiber diameter would increase with increasing concentration of the polymer solution and the flow rate. Therefore, the screen distance could also increase but the average electrical potential of the fibers diameter decreases. Electrospinning process can be conducted at higher electrical potentials, lower flow rate, nearer screen distance, and higher concentrations of dope.

Optimizing Process Variables to Control Fiber Diameter of Electrospun Polycaprolactone Nanofiber Using Factorial Design

2011 ◽  
Vol 1316 ◽  
Author(s):  
Saida P. Khan ◽  
Kadambari Bhasin ◽  
Golam M. Newaz
Keyword(s):  
Dielectric Constant ◽  
Factorial Design ◽  
Fiber Diameter ◽  
Polymer Concentration ◽  
Engineering Application ◽  
Electrospinning Process ◽  
Solution Flow Rate ◽  
Tissue Engineering Application ◽  
Electrospinning Technique ◽  
Solution Flow

ABSTRACTIn the electrospinning process, fibers ranging from 50 nm to 1000 nm or greater can be produced by applying an electric potential to a polymeric solution [1, 2]. Our group has studied the fabrication of electro-spun Poly-caprolactone (PCL) nanofiber consisting of a range of fiber diameter (nm-um) and pore sizes. PCL is a biocompatible, FDA approved and biodegradable [3, 4] polymer. As a solvent we have used 2,2,2-trifluoroethanol (TFE) for its biocompatibility, conductivity and high dielectric constant. The electrospinning technique consists of a simple setup with a number of variables working in a complex and unpredictable way. The variables affecting fiber diameter are polymer concentration in the solution, flow rate, applied voltage, tip to collector distance, diameter of the needle/capillary, polymer/solvent dielectric constant etc. In our study we have found that concentration of the solution and molecular weight of the polymer are the most important parameters for forming the nanofibers and viscosity is important for the fiber diameter. To optimize so many variables to control the fiber diameter, we have used the factorial design method. The study is important for the fabrication of biomimetic scaffold for vascular implant and tissue engineering application.

The Formation and Morphology of PVA Ferrogel Nanofibre by the Electrospinning Process

2010 ◽  
Vol 650 ◽  
pp. 361-366 ◽  
Author(s):  
Tao Yu Wan ◽  
Mohammad Chowdhury ◽  
George K. Stylios
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Polyvinyl Alcohol ◽  
Flow Rate ◽  
Electrospinning Process ◽  
Magnetic Sensors ◽  
Solution Flow Rate ◽  
Solution Flow ◽  
Fiber Mats ◽  
Scanning Electron

Aqueous solutions of polyvinyl alcohol (PVA) with FeCl3 were homogenously mixed and subsequently electrospun; and its characteristics were studied as a function of voltage, tip-target distance and solution flow rate. Fiber mats of (PVA)/FeCl3 composite, in the diameter of 700–1100 nm were prepared by electrospinning. Lower concentrations of solution tended to facilitate the formation of fibres with beads. With increasing concentration, the morphology was improved with smooth and uniform fibres and the increased fibre diameters in the nano range. Spinning voltage also had an important influence on the diameters of the nano fibres, while the collection distance affected fibre diameters. Nano fibres of smaller diameter were formed when lower voltages are applied. The morphology of the electrospun from PVA/FeCl3 nano fibres and their magnetic power was observed and analyzed by scanning electron microscopy (SEM).The fibres produced in this way could potentially be applied to manufacture magnetic sensors, flexible magnets.

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