scholarly journals Investigation of Functionalized Surface Charges of Thermoplastic Starch/Zinc Oxide Nanocomposite Films Using Polyaniline: The Potential of Improved Antibacterial Properties

Polymers ◽  
2021 ◽  
Vol 13 (3) ◽  
pp. 425
Author(s):  
Korakot Charoensri ◽  
Chatchai Rodwihok ◽  
Duangmanee Wongratanaphisan ◽  
Jung A. Ko ◽  
Jin Suk Chung ◽  
...  
Keyword(s):  
Zinc Oxide ◽  
Antibacterial Activity ◽  
Antibacterial Properties ◽  
Thermoplastic Starch ◽  
Nanocomposite Films ◽  
Resistant Bacteria ◽  
Surface Charges ◽  
Zno Nps ◽  
E Coli ◽  
Bionanocomposite Films

Improving the antibacterial activity of biodegradable materials is crucial for combatting widespread drug-resistant bacteria and plastic pollutants. In this work, we studied polyaniline (PANI)-functionalized zinc oxide nanoparticles (ZnO NPs) to improve surface charges. A PANI-functionalized ZnO NP surface was prepared using a simple impregnation technique. The PANI functionalization of ZnO successfully increased the positive surface charge of the ZnO NPs. In addition, PANI-functionalized ZnO improved mechanical properties and thermal stability. Besides those properties, the water permeability of the bionanocomposite films was decreased due to their increased hydrophobicity. PANI-functionalized ZnO NPs were applied to thermoplastic starch (TPS) films for physical properties and antibacterial studies using Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus). The PANI-functionalized ZnO bionanocomposite films exhibited excellent antibacterial activity for both E. coli (76%) and S. aureus (72%). This result suggests that PANI-functionalized ZnO NPs can improve the antibacterial activity of TPS-based bionanocomposite films.

scholarly journals Effect of Zinc Oxide Nanoparticles on Loaded Antibiotics Against Multidrug-Resistant Acinetobacter spp.

2021 ◽  
Vol 8 (2) ◽  
pp. 51-56
Author(s):  
Behnaz Shokrollahi ◽  
Akram Sadat Tabatabaee Bafroee ◽  
Tayebeh Saleh
Keyword(s):  
Zinc Oxide ◽  
Antibacterial Activity ◽  
Metal Oxide Nanoparticles ◽  
Antibacterial Properties ◽  
Multidrug Resistant ◽  
Diffusion Method ◽  
Resistant Bacteria ◽  
Oxide Nanoparticles ◽  
Zno Nps ◽  
X Ray

Background: Metal oxide nanoparticles (NPs) have shown promising efficacy for combating bacterial resistance due to their antibacterial properties. This research investigated the effect of zinc oxide NPs (ZnO-NPs) on the antibacterial activity of conventional antibiotics including ciprofloxacin (CIP), cefotaxime (CTX), and colistin (CST) against multidrug-resistant Acinetobacter isolates. Methods: The disc diffusion method was performed to detect the pattern of antibiotic resistance in isolates. The synthesized ZnO-NPs via the solvothermal method were characterized by field emission scanning electron microscopy (FESEM), X-ray diffraction (XRD), and energy-dispersive X-ray spectroscopy (EDS). Finally, the broth microdilution technique was conducted to demonstrate the antibacterial activity of CIP, CTX, and CST antibiotics with and without a sub-inhibitory concentration of ZnO-NPs. Results: XRD, EDS, and FESEM results confirmed the crystalline structure of ZnO-NPs, and the average size was 100±58.68 nm. All isolates were discovered to be of multidrug-resistant (MDR) type and fully susceptible to CST. The antibacterial activity of CTX and CIP was restored when combined with a sub-inhibitory level of ZnO-NPs (0.25 mg/L), and the highest activity was obtained at the concentrations of 32 µg/mL CTX and 8 µg/ mL CIP. Eventually, ZnO-NPs showed a synergistic effect on the antibacterial properties of CST against MDR Acinetobacter. Conclusions: This research indicated that the combination of ZnO-NPs with some common antibiotics can be considered as a novel strategy for reducing the spread of antibiotic-resistant bacteria.

scholarly journals Antibacterial Property of Composites of Reduced Graphene Oxide with Nano-Silver and Zinc Oxide Nanoparticles Synthesized Using a Microwave-Assisted Approach

2019 ◽  
Vol 20 (21) ◽  
pp. 5394 ◽  
Author(s):  
Yi-Huang Hsueh ◽  
Chien-Te Hsieh ◽  
Shu-Ting Chiu ◽  
Ping-Han Tsai ◽  
Chia-Ying Liu ◽  
...  
Keyword(s):  
Zinc Oxide ◽  
Antibacterial Activity ◽  
Graphene Oxide ◽  
Reductase Activity ◽  
Chemical Reduction ◽  
Membrane Integrity ◽  
Zno Nps ◽  
Nano Silver ◽  
E Coli ◽  
Microwave Assisted

Graphene oxide (GO) composites with various metal nanoparticles (NPs) are attracting increasing interest owing to their broad scope in biomedical applications. Here, microwave-assisted chemical reduction was used to deposit nano-silver and zinc oxide NPs (Ag and ZnO NPs) on the surface of reduced GO (rGO) at the following weight percentages: 5.34% Ag/rGO, 7.49% Ag/rGO, 6.85% ZnO/rGO, 16.45% ZnO/rGO, 3.47/34.91% Ag/ZnO/rGO, and 7.08/15.28% Ag/ZnO/rGO. These materials were tested for antibacterial activity, and 3.47/34.91% Ag/ZnO/rGO and 7.08/15.28% Ag/ZnO/rGO exhibited better antibacterial activity than the other tested materials against the gram-negative bacterium Escherichia coli K12. At 1000 ppm, both these Ag/ZnO/rGO composites had better killing properties against both E. coli K12 and the gram-positive bacterium Staphylococcus aureus SA113 than Ag/rGO and ZnO/rGO did. RedoxSensor flow cytometry showed that 3.47/34.91% Ag/ZnO/rGO and 7.08/15.28% Ag/ZnO/rGO decreased reductase activity and affected membrane integrity in the bacteria. At 100 ppm, these two composites affected membrane integrity more in E. coli, while 7.08/15.28% Ag/ZnO/rGO considerably decreased reductase activity in S. aureus. Thus, the 3.47/34.91% and 7.08%/15.28% Ag/ZnO/rGO nanocomposites can be applied not only as antibacterial agents but also in a variety of biomedical materials such as sensors, photothermal therapy, drug delivery, and catalysis, in the future.

scholarly journals New Organosilicon Composite Based on Borosiloxane and Zinc Oxide Nanoparticles Inhibits Bacterial Growth, but Does Not Have a Toxic Effect on the Development of Animal Eukaryotic Cells

Materials ◽  
2021 ◽  
Vol 14 (21) ◽  
pp. 6281
Author(s):  
Denis N. Chausov ◽  
Dmitriy E. Burmistrov ◽  
Alexander D. Kurilov ◽  
Nikolay F. Bunkin ◽  
Maxim E. Astashev ◽  
...  
Keyword(s):  
Zinc Oxide ◽  
Composite Material ◽  
Zinc Oxide Nanoparticles ◽  
Antibacterial Properties ◽  
Comprehensive Analysis ◽  
Oxide Nanoparticles ◽  
Oxygen Species ◽  
Zno Nps ◽  
E Coli ◽  
Positive Effect

The present study a comprehensive analysis of the antibacterial properties of a composite material based on borosiloxane and zinc oxide nanoparticles (ZnO NPs). The effect of the polymer matrix and ZnO NPs on the generation of reactive oxygen species, hydroxyl radicals, and long-lived oxidized forms of biomolecules has been studied. All variants of the composites significantly inhibited the division of E. coli bacteria and caused them to detach from the substrate. It was revealed that the surfaces of a composite material based on borosiloxane and ZnO NPs do not inhibit the growth and division of mammalians cells. It is shown in the work that the positive effect of the incorporation of ZnO NPs into borosiloxane can reach 100% or more, provided that the viscoelastic properties of borosiloxane with nanoparticles are retained.

scholarly journals Improvement of the UV Barrier and Antibacterial Properties of Crosslinked Pectin/Zinc Oxide Bionanocomposite Films

Polymers ◽  
2021 ◽  
Vol 13 (15) ◽  
pp. 2403
Author(s):  
Karina Dyasti Hari ◽  
Coralia V. Garcia ◽  
Gye-Hwa Shin ◽  
Jun-Tae Kim
Keyword(s):  
Zinc Oxide ◽  
Zinc Oxide Nanoparticles ◽  
Uv Light ◽  
Antibacterial Properties ◽  
Antibacterial Effects ◽  
Zno Nps ◽  
Elongation At Break ◽  
Light Barrier ◽  
Bionanocomposite Films ◽  
And Staphylococcus Aureus

Pectin-based antibacterial bionanocomposite films were prepared by crosslinking with calcium chloride (CaCl2) and mixing with zinc oxide nanoparticles (ZnO-NPs) at various concentrations (0.5%, 1%, and 1.5% w/w, based on pectin). Crosslinking with 1% CaCl2 significantly (p < 0.05) improved the tensile strength of the pectin films, although their elongation at break was decreased. The UV-light barrier property of the pectin/ZnO bionanocomposite films was significantly (p < 0.05) improved with increasing ZnO-NP concentrations. In addition, the bionanocomposite films incorporating 1.5% ZnO-NPs showed excellent antibacterial effects against both Escherichia coli and Staphylococcus aureus, inhibiting over 99% of the bacteria. Therefore, the developed crosslinked pectin/ZnO bionanocomposite films show great potential as active packaging materials with excellent UV-blocking and antibacterial properties.

scholarly journals Synergistic Effect of Glycyrrhizic Acid and ZnO/Palygorskite on Improving Chitosan-Based Films and Their Potential Application in Wound Healing

Polymers ◽  
2021 ◽  
Vol 13 (22) ◽  
pp. 3878
Author(s):  
Qian Zhang ◽  
Hong Zhang ◽  
Aiping Hui ◽  
Junjie Ding ◽  
Xinyue Liu ◽  
...  
Keyword(s):  
Synergistic Effect ◽  
Glycyrrhizic Acid ◽  
Wound Care ◽  
Antibacterial Properties ◽  
Nanocomposite Films ◽  
Resistant Bacteria ◽  
Promising Candidate ◽  
E Coli ◽  
Drug Resistant Bacteria ◽  
Solution Casting Method

The synergistic effect of chitosan (CS), glycyrrhizic acid (GA) and ZnO/palygorskite (ZnO/PAL) as potential wound dressing was evaluated in the form of films by the solution casting method. The nanocomposite films were well-characterized with ATR-FTIR, XRD and SEM to explore the interactions between CS, GA and ZnO/PAL. Physical, mechanical and antibacterial properties of the nanocomposite films were systematically investigated for their reliability in end-up utilization. Importantly, it was found that the presence of PAL in the films provided enhanced mechanical properties, whereas CS, GA and ZnO supplied a broad-spectrum antibacterial activity, especially for drug-resistant bacteria such as ESBL—E. coli and MRSA. Overall, this research demonstrated that the prepared films can be a promising candidate for wound-care materials.

scholarly journals Antibacterial Potential of Biosynthesized Zinc Oxide Nanoparticles against Poultry-Associated Foodborne Pathogens: An In Vitro Study

Animals ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 2093
Author(s):  
Hidayat Mohd Yusof ◽  
Nor’Aini Abdul Rahman ◽  
Rosfarizan Mohamad ◽  
Uswatun Hasanah Zaidan ◽  
Anjas Asmara Samsudin
Keyword(s):  
Zinc Oxide ◽  
Antibacterial Activity ◽  
Foodborne Pathogens ◽  
Zinc Oxide Nanoparticles ◽  
Resistant Bacteria ◽  
Oxide Nanoparticles ◽  
Zno Nps ◽  
Wide Range ◽  
Antibacterial Potential

Since the emergence of multidrug-resistant bacteria in the poultry industry is currently a serious threat, there is an urgent need to develop a more efficient and alternative antibacterial substance. Zinc oxide nanoparticles (ZnO NPs) have exhibited antibacterial efficacy against a wide range of microorganisms. Although the in vitro antibacterial activity of ZnO NPs has been studied, little is known about the antibacterial mechanisms of ZnO NPs against poultry-associated foodborne pathogens. In the present study, ZnO NPs were successfully synthesized using Lactobacillus plantarum TA4, characterized, and their antibacterial potential against common avian pathogens (Salmonella spp., Escherichia coli, and Staphylococcus aureus) was investigated. Confirmation of ZnO NPs by UV-Visual spectroscopy showed an absorption band center at 360 nm. Morphologically, the synthesized ZnO NPs were oval with an average particle size of 29.7 nm. Based on the dissolution study of Zn2+, ZnO NPs released more ions than their bulk counterparts. Results from the agar well diffusion assay indicated that ZnO NPs effectively inhibited the growth of the three poultry-associated foodborne pathogens. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were assessed using various concentrations of ZnO NPs, which resulted in excellent antibacterial activity as compared to their bulkier counterparts. S. aureus was more susceptible to ZnO NPs compared to the other tested bacteria. Furthermore, the ZnO NPs demonstrated substantial biofilm inhibition and eradication. The formation of reactive oxygen species (ROS) and cellular material leakage was quantified to determine the underlying antibacterial mechanisms, whereas a scanning electron microscope (SEM) was used to examine the morphological changes of tested bacteria treated with ZnO NPs. The findings suggested that ROS-induced oxidative stress caused membrane damage and bacterial cell death. Overall, the results demonstrated that ZnO NPs could be developed as an alternative antibiotic in poultry production and revealed new possibilities in combating pathogenic microorganisms.

scholarly journals Effect of Synthesis Temperature on the Size of ZnO Nanoparticles Derived from Pineapple Peel Extract and Antibacterial Activity of ZnO–Starch Nanocomposite Films

Nanomaterials ◽  
2020 ◽  
Vol 10 (6) ◽  
pp. 1061 ◽  
Author(s):  
Hasbullah Hassan Basri ◽  
Rosnita A. Talib ◽  
Rashidah Sukor ◽  
Siti Hajar Othman ◽  
Hidayah Ariffin
Keyword(s):  
Electron Microscopy ◽  
Zinc Oxide ◽  
Antibacterial Activity ◽  
Zno Nanoparticles ◽  
Synthesis Temperature ◽  
Nanocomposite Films ◽  
Zno Nps ◽  
Gram Positive ◽  
X Ray ◽  
Pineapple Peel

This research investigated the effect of synthesis temperature on the size and shape of zinc oxide (ZnO) nanoparticles (NPs) synthesized using pineapple peel waste and antibacterial activity of ZnO NPs in starch films. Zinc oxide NPs synthesized at different temperatures were characterized by Fourier transform infrared spectroscopy, X-ray diffraction analysis, field-emission scanning electron microscopy, energy-dispersive X-ray spectroscopy, and transmission electron microscopy. Micrographs of ZnO NPs synthesized at 28 and 60 °C showed that synthesis temperature affected the sizes and shapes of ZnO NPs. The non-heated (28 °C) condition resulted in NPs with diameters in the range of 8–45 nm with a mixture of spherical and rod shapes, whereas the heated (60 °C) condition led to NPs with diameters in the range of 73–123 nm with flower rod shapes. The ZnO–starch nanocomposite films incorporated with 1, 3, and 5 wt.% ZnO NPs were prepared via a film casting method. The antibacterial activity of the films against Gram-positive and Gram-negative bacteria was investigated using the disc diffusion method. The results showed an increase in the inhibition zone for Gram-positive bacteria, particularly Bacillus subtilis, when the concentration of ZnO NPs incorporated in the film was increased from 1 to 5 wt.%.

scholarly journals Green Synthesis of Zinc Oxide Nanoparticles and their Antibacterial Properties using Plant Extract of Aristolochia elegans

2020 ◽  
Vol 32 (10) ◽  
pp. 2589-2593
Author(s):  
Juhi Aggarwal ◽  
Tanveer Alam
Keyword(s):  
Zinc Oxide ◽  
Antibacterial Activity ◽  
Zinc Oxide Nanoparticles ◽  
Leaf Extract ◽  
Antibacterial Property ◽  
Antibacterial Properties ◽  
Oxide Nanoparticles ◽  
Gram Negative ◽  
E Coli ◽  
Ft Ir

Present paper deals with the synthesis of zinc oxide nanoparticles (ZnONPs) using leaf extract of Aristolochia elegans and study of antibacterial property for some human bacterial pathogens. The ZnONPs synthesized were characterized using UV-Vis, FT-IR, XRD, EDX, TEM and SEM techniques. The synthesized ZnONP having a crystallite size of 20.1 nm exhibited a distinct absorption peak maxima at 358 nm. The ZnONPs synthesized using the extract of A. elegans have shown antibacterial activity against M. luteus, S. aureus (Gram-positive), E. coli and P. aeruginosa (Gram-negative).

scholarly journals Enhanced antibacterial activity of capped zinc oxide nanoparticles: A step towards the control of clinical bovine mastitis

2019 ◽  
Vol 12 (8) ◽  
pp. 1225-1232 ◽  
Author(s):  
H. F. Hozyen ◽  
E. S. Ibrahim ◽  
E. A. Khairy ◽  
S. I. El-Dek
Keyword(s):  
Zinc Oxide ◽  
Antibacterial Activity ◽  
Zinc Oxide Nanoparticles ◽  
Bovine Mastitis ◽  
Clinical Mastitis ◽  
Combustion Reaction ◽  
Oxide Nanoparticles ◽  
Zno Nps ◽  
E Coli ◽  
Auto Combustion

Background and Aim: Bovine mastitis is the costliest prevalent disease in the dairy sector due to the limitations of conventional treatments. Zinc oxide nanoparticles (ZnO-NPs) have been regarded as safe and economical antibacterial candidates against several microorganisms, but the tendency of these particles to aggregate is a major barrier to their application. This study aimed to enhance the antibacterial efficiency of ZnO-NPs against some bacterial agents, causing bovine mastitis. Materials and Methods: A total of 24 milk samples out of 300 cases from Nubaria farm, Beheira Governorate, Egypt, were collected from cows with clinical mastitis. ZnO-NPs were fabricated by a sonochemical method using starch as a capping agent and by an auto-combustion reaction using glycine as a fuel. The two preparations of synthesized ZnO-NPs at different concentrations were assessed for their antimicrobial activities in vitro against Staphylococcus aureus, Escherichia coli, and Klebsiella pneumoniae isolated from milk of affected cows. Results: Sonochemically synthesized capped ZnO-NPs were dispersed and non-agglomerated in comparison with aggregated uncapped ZnO-NPs prepared by an auto-combustion reaction. Capped dispersed ZnO-NPs showed higher antibacterial activity against S. aureus, E. coli, and K. pneumoniae than particles synthesized by the auto-combustion reaction at same concentrations. However, the zone of inhibition for dispersed and agglomerated ZnO-NPs was concentration-dependent. In addition, Gram-positive S. aureus exhibited higher resistance to ZnO-NPs synthesized by both methods than Gram-negative E. coli and K. pneumoniae. Conclusion: Dispersed, non-agglomerated ZnO-NPs fabricated using starch as a capping agent under sonochemical irradiation could potentially be regarded as highly effective and inexpensive antimicrobial agents against S. aureus, E. coli, and K. pneumoniae for the management of bovine mastitis. Keywords: antibacterial activity, clinical mastitis, dairy cows, zinc oxide nanoparticles.

The use of zinc oxide nanoparticles to enhance the antibacterial properties of light-activated polydimethylsiloxane containing crystal violet

RSC Advances ◽  
2015 ◽  
Vol 5 (12) ◽  
pp. 8806-8813 ◽  
Author(s):  
Ekrem Ozkan ◽  
Feyza Tunali Ozkan ◽  
Elaine Allan ◽  
Ivan P. Parkin
Keyword(s):  
Zinc Oxide ◽  
Antibacterial Activity ◽  
Crystal Violet ◽  
Zinc Oxide Nanoparticles ◽  
Antibacterial Properties ◽  
Oxide Nanoparticles ◽  
Step Method ◽  
E Coli ◽  
Modified Polymer

Crystal violet–ZnO mixtures were incorporated into PDMS by a simple two step method. The modified polymer demonstrated significant antibacterial activity againstE. coliandS. aureus, showing possibly the most potent light-induced antibacterial polymer reported to date.

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