scholarly journals Antibacterial Property of Composites of Reduced Graphene Oxide with Nano-Silver and Zinc Oxide Nanoparticles Synthesized Using a Microwave-Assisted Approach

2019 ◽  
Vol 20 (21) ◽  
pp. 5394 ◽  
Author(s):  
Yi-Huang Hsueh ◽  
Chien-Te Hsieh ◽  
Shu-Ting Chiu ◽  
Ping-Han Tsai ◽  
Chia-Ying Liu ◽  
...  
Keyword(s):  
Zinc Oxide ◽  
Antibacterial Activity ◽  
Graphene Oxide ◽  
Reductase Activity ◽  
Chemical Reduction ◽  
Membrane Integrity ◽  
Zno Nps ◽  
Nano Silver ◽  
E Coli ◽  
Microwave Assisted

Graphene oxide (GO) composites with various metal nanoparticles (NPs) are attracting increasing interest owing to their broad scope in biomedical applications. Here, microwave-assisted chemical reduction was used to deposit nano-silver and zinc oxide NPs (Ag and ZnO NPs) on the surface of reduced GO (rGO) at the following weight percentages: 5.34% Ag/rGO, 7.49% Ag/rGO, 6.85% ZnO/rGO, 16.45% ZnO/rGO, 3.47/34.91% Ag/ZnO/rGO, and 7.08/15.28% Ag/ZnO/rGO. These materials were tested for antibacterial activity, and 3.47/34.91% Ag/ZnO/rGO and 7.08/15.28% Ag/ZnO/rGO exhibited better antibacterial activity than the other tested materials against the gram-negative bacterium Escherichia coli K12. At 1000 ppm, both these Ag/ZnO/rGO composites had better killing properties against both E. coli K12 and the gram-positive bacterium Staphylococcus aureus SA113 than Ag/rGO and ZnO/rGO did. RedoxSensor flow cytometry showed that 3.47/34.91% Ag/ZnO/rGO and 7.08/15.28% Ag/ZnO/rGO decreased reductase activity and affected membrane integrity in the bacteria. At 100 ppm, these two composites affected membrane integrity more in E. coli, while 7.08/15.28% Ag/ZnO/rGO considerably decreased reductase activity in S. aureus. Thus, the 3.47/34.91% and 7.08%/15.28% Ag/ZnO/rGO nanocomposites can be applied not only as antibacterial agents but also in a variety of biomedical materials such as sensors, photothermal therapy, drug delivery, and catalysis, in the future.

scholarly journals Investigation of Functionalized Surface Charges of Thermoplastic Starch/Zinc Oxide Nanocomposite Films Using Polyaniline: The Potential of Improved Antibacterial Properties

Polymers ◽  
2021 ◽  
Vol 13 (3) ◽  
pp. 425
Author(s):  
Korakot Charoensri ◽  
Chatchai Rodwihok ◽  
Duangmanee Wongratanaphisan ◽  
Jung A. Ko ◽  
Jin Suk Chung ◽  
...  
Keyword(s):  
Zinc Oxide ◽  
Antibacterial Activity ◽  
Antibacterial Properties ◽  
Thermoplastic Starch ◽  
Nanocomposite Films ◽  
Resistant Bacteria ◽  
Surface Charges ◽  
Zno Nps ◽  
E Coli ◽  
Bionanocomposite Films

Improving the antibacterial activity of biodegradable materials is crucial for combatting widespread drug-resistant bacteria and plastic pollutants. In this work, we studied polyaniline (PANI)-functionalized zinc oxide nanoparticles (ZnO NPs) to improve surface charges. A PANI-functionalized ZnO NP surface was prepared using a simple impregnation technique. The PANI functionalization of ZnO successfully increased the positive surface charge of the ZnO NPs. In addition, PANI-functionalized ZnO improved mechanical properties and thermal stability. Besides those properties, the water permeability of the bionanocomposite films was decreased due to their increased hydrophobicity. PANI-functionalized ZnO NPs were applied to thermoplastic starch (TPS) films for physical properties and antibacterial studies using Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus). The PANI-functionalized ZnO bionanocomposite films exhibited excellent antibacterial activity for both E. coli (76%) and S. aureus (72%). This result suggests that PANI-functionalized ZnO NPs can improve the antibacterial activity of TPS-based bionanocomposite films.

scholarly journals SYNTHESIS OF ZINC OXIDE/GRAPHENE OXIDE NANOCOMPOSITES AS ANTIBACTERIAL MATERIALS AGAINST STAPHYLOCOCCUS AUREUS AND ESCHERICHIA COLI

2018 ◽  
Vol 55 (1B) ◽  
pp. 266 ◽  
Author(s):  
Nguyen Huu Hieu
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Zinc Oxide ◽  
Antibacterial Activity ◽  
Graphene Oxide ◽  
Surface Area ◽  
Transmission Electron Microscopy Image ◽  
Precipitation Method ◽  
Diffusion Method ◽  
E Coli

New materials with good antibacterial activity and less toxicity to other species have attracted numerous research interests. Modified Hummers method was used for preparing graphene oxide (GO). Zinc oxide/graphene oxide (ZnO/GO) nanocomposites were synthesized with three different ratios (0.5:1, 1:1, and 2:1) by solution precipitation method. The ZnO/GO nanocomposites were characterized by Fourier transform infrared spectroscopy, X–ray diffraction, Raman spectroscopy, Brunauer–Emmett–Tellerspecific surface area, and transmission electron microscopy image. The characterization results showed that ZnO nanoparticles with a mean size of 12–18 nm were randomly decorated on the surfaces and edges of GO sheets. ZnO/GO 1:1 with a high specific surface area of 65 m2/g was obtained. The antibacterial activity of ZnO, GO, and ZnO/GO was tested against Gram negative bacteria escherichia coli (E. coli) and Gram positive bacteria staphylococcus aureus (S. aureus) using well diffusion method. The test results confirmed that antibacterial activity of ZnO/GO was higher than that of GO and ZnO. Additionally, the ZnO/GO with the ratio of 1:1 is the strongest activity and more active against S. aureus than against E. coli and minimal inhibitory concentration (MIC) value of ZnO/GO 1:1 is 80 µg/mL for S. aureus and 160 µg/mL for E. coli. This novel nanocomposite could be used as a potential material for antimicrobial application.

scholarly journals Size-Dependent Antibacterial Activity of Silver Nanoparticle-Loaded Graphene Oxide Nanosheets

Nanomaterials ◽  
2020 ◽  
Vol 10 (6) ◽  
pp. 1207 ◽  
Author(s):  
Thi Truong ◽  
Selvaraj Kumar ◽  
Yu-Tzu Huang ◽  
Dave Chen ◽  
Yu-Kuo Liu ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Graphene Oxide ◽  
Membrane Integrity ◽  
Antibacterial Properties ◽  
Ag Nps ◽  
E Coli ◽  
Size Dependent ◽  
Transmission Electron ◽  
Combined Action ◽  
Bacterial Cell Membrane

A series of graphene oxide (GO) suspensions with different particle sizes (<100 nm, ~100 nm, ~1 µm and >1 µm) were successfully fabricated after 0, 30, 60 and 120 min of sonication, respectively. The antibacterial properties of GO suspensions showed that >1 µm GO size resulted in a loss of nearly 50% of bacterial viability, which was higher than treatment by ~100 nm GO size (25%) towards Escherichia coli (E. coli). Complete entrapment of bacteria by the larger GO was observed in transmission electron microscopy (TEM). Silver nanoparticles (Ag NPs) were doped onto GO samples with different lateral sizes to form GO–Ag NP composites. Resulting larger GO–Ag NPs showed higher antibacterial activity than smaller GO–Ag NPs. As observed by Fourier transform infrared spectroscopy (FTIR), the interaction between E. coli and GO occurred mainly at the outer membrane, where membrane amino acids interact with hydroxyl and epoxy groups. The reactive oxygen species (ROS) and the considerable penetration of released Ag+ into the inner bacterial cell membrane result in loss of membrane integrity and damaged morphology. The present work improves the combined action of GO size effect with constant Ag loadings for potential antibacterial activity.

scholarly journals Enhanced antibacterial activity of capped zinc oxide nanoparticles: A step towards the control of clinical bovine mastitis

2019 ◽  
Vol 12 (8) ◽  
pp. 1225-1232 ◽  
Author(s):  
H. F. Hozyen ◽  
E. S. Ibrahim ◽  
E. A. Khairy ◽  
S. I. El-Dek
Keyword(s):  
Zinc Oxide ◽  
Antibacterial Activity ◽  
Zinc Oxide Nanoparticles ◽  
Bovine Mastitis ◽  
Clinical Mastitis ◽  
Combustion Reaction ◽  
Oxide Nanoparticles ◽  
Zno Nps ◽  
E Coli ◽  
Auto Combustion

Background and Aim: Bovine mastitis is the costliest prevalent disease in the dairy sector due to the limitations of conventional treatments. Zinc oxide nanoparticles (ZnO-NPs) have been regarded as safe and economical antibacterial candidates against several microorganisms, but the tendency of these particles to aggregate is a major barrier to their application. This study aimed to enhance the antibacterial efficiency of ZnO-NPs against some bacterial agents, causing bovine mastitis. Materials and Methods: A total of 24 milk samples out of 300 cases from Nubaria farm, Beheira Governorate, Egypt, were collected from cows with clinical mastitis. ZnO-NPs were fabricated by a sonochemical method using starch as a capping agent and by an auto-combustion reaction using glycine as a fuel. The two preparations of synthesized ZnO-NPs at different concentrations were assessed for their antimicrobial activities in vitro against Staphylococcus aureus, Escherichia coli, and Klebsiella pneumoniae isolated from milk of affected cows. Results: Sonochemically synthesized capped ZnO-NPs were dispersed and non-agglomerated in comparison with aggregated uncapped ZnO-NPs prepared by an auto-combustion reaction. Capped dispersed ZnO-NPs showed higher antibacterial activity against S. aureus, E. coli, and K. pneumoniae than particles synthesized by the auto-combustion reaction at same concentrations. However, the zone of inhibition for dispersed and agglomerated ZnO-NPs was concentration-dependent. In addition, Gram-positive S. aureus exhibited higher resistance to ZnO-NPs synthesized by both methods than Gram-negative E. coli and K. pneumoniae. Conclusion: Dispersed, non-agglomerated ZnO-NPs fabricated using starch as a capping agent under sonochemical irradiation could potentially be regarded as highly effective and inexpensive antimicrobial agents against S. aureus, E. coli, and K. pneumoniae for the management of bovine mastitis. Keywords: antibacterial activity, clinical mastitis, dairy cows, zinc oxide nanoparticles.

Well-Aligned Graphene Oxide Nanosheets Decorated with Zinc Oxide Nanocrystals for High Performance Photocatalytic Application

2015 ◽  
Vol 14 (03) ◽  
pp. 1550007 ◽  
Author(s):  
K. Kaviyarasu ◽  
C. Maria Magdalane ◽  
E. Manikandan ◽  
M. Jayachandran ◽  
R. Ladchumananandasivam ◽  
...  
Keyword(s):  
Zinc Oxide ◽  
Graphene Oxide ◽  
High Performance ◽  
Chemical Reduction ◽  
Visible Region ◽  
Single Layer ◽  
Single Layer Graphene ◽  
Graphene Oxides ◽  
X Ray ◽  
Nanoscale Structures

Graphene oxide (GO) nanosheets modified with zinc oxide nanocrystals were achieved by a green wet-chemical approach. As-obtained products were characterized by XRD, Raman spectra, XPS, HR-TEM, EDS, PL and Photocatalytic studies. XRD studies indicate that the GO nanosheet have the same crystal structure found in hexagonal form of ZnO . The enhanced Raman spectrum of 2D bands confirmed formation of single layer graphene oxides. The gradual photocatalytic reduction of the GO nanosheet in the GO : ZnO suspension of ethanol was studied by using X-ray photoelectron (XPS) spectroscopy. The nanoscale structures were observed and confirmed using high resolution transmission electron microscopy (HR-TEM). The evolution of the elemental composition, especially the various numbers of layers were determined from energy dispersive X-ray spectra (EDS). PL properties of GO : ZnO nanosheet were found to be dependent on the growth condition and the resultant morphology revealed that GO nanosheet were highly transparent in the visible region. The photocatalytic performance of GO : ZnO nanocomposites was performed under UV irradiation. Therefore, the ZnO nanocrystals in the GO : ZnO composite could be applied in gradual chemical reduction and consequently tuning the electrical conductivity of the graphene oxide nanosheet.

scholarly journals Synthesis of zinc oxide and silver nanoparticles using ficus palmata - Forssk leaf extracts and assessment of antibacterial activity

2020 ◽  
Vol 26 (6) ◽  
pp. 200454-0
Author(s):  
Sabaoon Shamshad ◽  
Jamshaid Rashid ◽  
Ihsan-ul-haq ◽  
Naseem Iqbal ◽  
Saif Ullah Awan
Keyword(s):  
Staphylococcus Aureus ◽  
Silver Nanoparticles ◽  
Zinc Oxide ◽  
Antibacterial Activity ◽  
Zinc Oxide Nanoparticles ◽  
Inhibitory Concentration ◽  
Oxide Nanoparticles ◽  
Leaf Extracts ◽  
E Coli ◽  
Ficus Palmata

Multidrug resistance of bacteria is an emerging human health hazard and warrants development of novel antibacterial agents with more effective mode of action. Here, zinc oxide and silver nanomaterials were prepared using Ficus palmata Forssk leaf extract with efficient antibacterial activity. SEM coupled with EDS confirmed the spherical symmetry with average particle diameter 50 to 65 nm while the XRD confirmed crystalline face centered cubic structure of silver and hexagonal crystallize phase of zinc oxide nanoparticles. Antibacterial activity was evaluated for 8 pathogenic bacterial strains including 3 drug resistant pathogenic strains. The nanoparticles showed enhanced growth inhibition for resistant strains in comparison with the broad-spectrum antibiotics i.e. roxithromycin and cefixime. Minimum inhibitory concentration in μg.mL<sup>-1</sup> of silver nanoparticles was found to be as low as 33.3 for resistant Streptococcus haemolyticus; 11.1 for Staphylococcus aureus and E Coli; and 3.7 μg.mL<sup>-1</sup> for resistant Pseudomonas aeruginosa. Similarly, the minimum inhibitory concentration of zinc oxide nanoparticles was found to be 100 μg.mL<sup>-1</sup> against resistant Streptococcus haemolyticus and Staphylococcus aureus; 11.1 μg.mL<sup>-1</sup> for resistant Pseudomonas aeruginosa; and 3.7 μg.mL<sup>-1</sup> against resistant E coli. Ficus palmata Forssk leaf extracts can be explored effectively for synthesizing active antibacterial nanomaterials as a non-toxic and environmentally benign synthesis route.

Reducing Pathogens by Using Zinc Oxide Nanoparticles and Acetic Acid in Sheep Meat

2014 ◽  
Vol 77 (9) ◽  
pp. 1599-1604 ◽  
Author(s):  
MAHBOUBEH MIRHOSSEINI ◽  
VAHID ARJMAND
Keyword(s):  
Zinc Oxide ◽  
Antibacterial Activity ◽  
Acetic Acid ◽  
Zno Nanoparticles ◽  
Pathogenic Bacteria ◽  
Inhibitory Effect ◽  
Initial Growth ◽  
Practical Applications ◽  
E Coli ◽  
Sheep Meat

Practical applications of different concentrations (0, 1, 2, 4, 6, and 8 mM) of zinc oxide (ZnO) suspensions containing 1% acetic acid were investigated against the pathogenic bacteria Listeria monocytogenes, Escherichia coli, Staphylococcus aureus, and Bacillus cereus. ZnO suspensions (0, 1, 3, 6, and 8 mM) containing acetic acid had a significant inhibitory effect on the growth of L. monocytogenes, E. coli, and S. aureus during 12 h of incubation, and the 8 mM suspensions of ZnO were the most effective against all the strains. These data suggested that the antibacterial activity of ZnO was concentration dependent. Thus, 6 and 8 mM ZnO were selected for further studies in meat. ZnO nanoparticles reduced initial growth of all inoculated strains in meat. To our knowledge, this is the first report describing the antibacterial activity of ZnO nanoparticles in meat and indicates the potential of these nanoparticles as an antibacterial agent in the food industry.

scholarly journals Graphene oxide decorated with zinc oxide nanoflower, silver and titanium dioxide nanoparticles: fabrication, characterization, DNA interaction, and antibacterial activity

RSC Advances ◽  
2019 ◽  
Vol 9 (7) ◽  
pp. 3704-3714 ◽  
Author(s):  
Nagi El-Shafai ◽  
Mohamed E. El-Khouly ◽  
Maged El-Kemary ◽  
Mohamed Ramadan ◽  
Ibrahim Eldesoukey ◽  
...  
Keyword(s):  
Zinc Oxide ◽  
Antibacterial Activity ◽  
Titanium Dioxide ◽  
Graphene Oxide ◽  
Titanium Dioxide Nanoparticles ◽  
Dna Interaction

The fabrication, characterization, and antibacterial activity of novel nanocomposites based on graphene oxide (GO) nanosheets decorated with silver, titanium dioxide nanoparticles, and zinc oxide nanoflowers were examined.

Synthesis and antibacterial activity of silver-copper nano-composites formed by microwave assisted chemical reduction

2020 ◽  
Author(s):  
Amal Kallivalappil Puthalath ◽  
Sanya Hazel ◽  
Revathi Kottappara ◽  
Asha Srinivasan ◽  
Baiju K. Vijayan ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Chemical Reduction ◽  
Nano Composites ◽  
Microwave Assisted ◽  
Silver Copper
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