scholarly journals Interaction of Proteins with a Planar Poly(acrylic acid) Brush: Analysis by Quartz Crystal Microbalance with Dissipation Monitoring (QCM-D)

Polymers ◽  
2020 ◽  
Vol 13 (1) ◽  
pp. 122
Author(s):  
Jacek Walkowiak ◽  
Michael Gradzielski ◽  
Stefan Zauscher ◽  
Matthias Ballauff
Keyword(s):  
Acrylic Acid ◽  
Quartz Crystal Microbalance ◽  
Quartz Crystal ◽  
Water Contact ◽  
Strong Adsorption ◽  
Ft Ir ◽  
Calorimetric Studies ◽  
Adsorption Desorption ◽  
Contact Angle Analysis ◽  
Poly Acrylic Acid

We describe the preparation of a poly(acrylic acid) (PAA) brush, polymerized by atom transfer radical polymerization (ATRP) of tert-butyl acrylate (tBA) and subsequent acid hydrolysis, on the flat gold surfaces of quartz-crystal microbalance (QCM) crystals. The PAA brushes were characterized by Fourier transform infrared (FT-IR) spectroscopy, ellipsometry and water contact angle analysis. The interaction of the PAA brushes with human serum albumin (HSA) was studied for a range of ionic strengths and pH conditions by quartz-crystal microbalance with dissipation monitoring (QCM-D). The quantitative analysis showed a strong adsorption of protein molecules onto the PAA brush. By increasing the ionic strength, we were able to release a fraction of the initially bound HSA molecules. This finding highlights the importance of counterions in the polyelectrolyte-mediated protein adsorption/desorption. A comparison with recent calorimetric studies related to the binding of HSA to polyelectrolytes allowed us to fully analyze the QCM data based on the results of the thermodynamic analysis of the binding process.

scholarly journals Signal enhancement in ligand–receptor interactions using dynamic polymers at quartz crystal microbalance sensors

The Analyst ◽  
2016 ◽  
Vol 141 (13) ◽  
pp. 3993-3996 ◽  
Author(s):  
Gunnar Dunér ◽  
Henrik Anderson ◽  
Zhichao Pei ◽  
Björn Ingemarsson ◽  
Teodor Aastrup ◽  
...  
Keyword(s):  
Acrylic Acid ◽  
Quartz Crystal Microbalance ◽  
Quartz Crystal ◽  
Signal Enhancement ◽  
Interaction Studies ◽  
Receptor Interactions ◽  
Poly Acrylic Acid

The signal enhancement properties of QCM sensors based on dynamic, biotinylated poly(acrylic acid) brushes has been studied in interaction studies with an anti-biotin Fab fragment.

Nanoassembled Thin Film Gas Sensors. III. Sensitive Detection of Amine Odors Using TiO2/Poly(acrylic acid) Ultrathin Film Quartz Crystal Microbalance Sensors

2010 ◽  
Vol 82 (6) ◽  
pp. 2228-2236 ◽  
Author(s):  
Seung-Woo Lee ◽  
Naoki Takahara ◽  
Sergiy Korposh ◽  
Do-Hyeon Yang ◽  
Kiyoshi Toko ◽  
...  
Keyword(s):  
Thin Film ◽  
Acrylic Acid ◽  
Quartz Crystal Microbalance ◽  
Gas Sensors ◽  
Quartz Crystal ◽  
Ultrathin Film ◽  
Sensitive Detection ◽  
Poly Acrylic Acid

scholarly journals Hysteretic Swelling/Deswelling of Polyelectrolyte Brushes and Bilayer Films in Response to Changes in pH and Salt Concentration

Polymers ◽  
2021 ◽  
Vol 13 (5) ◽  
pp. 812
Author(s):  
Nisha Hollingsworth ◽  
Ronald G. Larson
Keyword(s):  
Acrylic Acid ◽  
Quartz Crystal Microbalance ◽  
Salt Concentration ◽  
Quartz Crystal ◽  
Ethylene Imine ◽  
Polyelectrolyte Brushes ◽  
Bilayer Films ◽  
Poly Ethylene ◽  
Poly Acrylic Acid

Abstract We use a quartz crystal microbalance with dissipation (QCM-D) to investigate the swelling/de-swelling and hysteresis in brushes of weakly ionizable polyanion poly(acrylic acid) (PAA) brushes and bilayers containing a PAA brush and a poly(ethylene imine) (PEI) overlayer [...]

Salt- and pH-induced swelling of a poly(acrylic acid) brush via quartz crystal microbalance w/dissipation (QCM-D)

Soft Matter ◽  
2019 ◽  
Vol 15 (39) ◽  
pp. 7838-7851 ◽  
Author(s):  
Nisha R. Hollingsworth ◽  
Sabina I. Wilkanowicz ◽  
Ronald G. Larson
Keyword(s):  
Acrylic Acid ◽  
Quartz Crystal Microbalance ◽  
Quartz Crystal ◽  
Swelling Behavior ◽  
Poly Acrylic Acid

Poly(arylic acid) brushes are thoroughly investigated using QCM-D to elucidate their swelling behavior in the presence of salt.

Magnetic Nanoparticles Derivatized with Dual Functional Groups for Environmental Applications

2010 ◽  
Vol 148-149 ◽  
pp. 949-952
Author(s):  
Hai Bo He ◽  
Qing Zhong Guo
Keyword(s):  
Magnetic Nanoparticles ◽  
Environmental Remediation ◽  
Infrared Spectrometry ◽  
X Ray Diffraction ◽  
Water Contact ◽  
Dual Functional ◽  
New Material ◽  
Ft Ir ◽  
Contact Angle Analysis ◽  
Effective Sorbent

In this study, the magnetic nanoparticles derivatized with dual functional moieties of dodecyl and mercapto were prepared, which characterized by X-ray diffraction (XRD), scanning electron microscope(SEM), surface area and pore size determination, fourier transform infrared spectrometry (FT-IR) and water contact angle analysis. The new material was proved to be an effective sorbent for environmental remediation.

Polyethylene-co-acrylic Acid as Coating for Biosensor Application:  A Quartz Crystal Microbalance Study

Langmuir ◽  
2002 ◽  
Vol 18 (25) ◽  
pp. 9932-9936 ◽  
Author(s):  
Kok Ting Chong ◽  
Xiaodi Su ◽  
Edmund J. D. Lee ◽  
S. J. O'Shea
Keyword(s):  
Acrylic Acid ◽  
Quartz Crystal Microbalance ◽  
Quartz Crystal ◽  
Biosensor Application

Immobilization of urease in poly(1-vinyl imidazole)/poly(acrylic acid) network

2010 ◽  
Vol 64 (1) ◽  
pp. 1-7 ◽  
Author(s):  
Mehmet Şenel ◽  
Agah Coşkun ◽  
M. Fatih Abasıyanık ◽  
Ayhan Bozkurt
Keyword(s):  
Acrylic Acid ◽  
Enzyme Immobilization ◽  
Storage Stability ◽  
Polymer Network ◽  
Ft Ir ◽  
Basic Characteristics ◽  
And Storage ◽  
Menten Constant ◽  
Ft Ir Spectroscopy ◽  
Poly Acrylic Acid

AbstractIn this study, urease was immobilized in a polymer network obtained by complexation of poly(1-vinyl imidazole) (PVI) with poly(acrylic acid) (PAA). Preparation of the polymer network was monitored by FT-IR spectroscopy. Scanning electron microscopy (SEM) revealed that enzyme immobilization had a strong effect on film morphology. Proton conductivity of the PVI/PAA network was measured via impedance spectroscopy under humidified conditions. Values of the Michaelis-Menten constant (K M) for immobilized urease were higher than for the free enzyme, indicating a decreased affinity of the enzyme to its substrate. The basic characteristics (pHopt, pHstability, T opt, T stability, reusability, and storage stability) of immobilized urease were determined. The results show that the PAA/PVI polymer network is suitable for enzyme immobilization.

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