scholarly journals Biological Effects of Polyrotaxane Surfaces on Cellular Responses of Fibroblast, Preosteoblast and Preadipocyte Cell Lines

Polymers ◽  
2020 ◽  
Vol 12 (4) ◽  
pp. 924
Author(s):  
Hiroki Masuda ◽  
Yoshinori Arisaka ◽  
Ruriko Sekiya-Aoyama ◽  
Tetsuya Yoda ◽  
Nobuhiko Yui
Keyword(s):  
Cellular Proliferation ◽  
Biological Effects ◽  
Cellular Responses ◽  
Structural Features ◽  
Cellular Adhesion ◽  
Cell Type ◽  
Poly Ethylene Glycol ◽  
Tissue Culture Polystyrene ◽  
Proliferation And Differentiation ◽  
Poly Ethylene

Biointerfaces based on polyrotaxane (PRX), consisting of α-cyclodextrins (α-CDs) threaded on a poly(ethylene glycol) (PEG) chain, are promising functionalized platforms for culturing cells. PRXs are characterized by the molecular mobility of constituent molecules where the threading α-CDs can move and rotate along the PEG chain. Taking advantage of this mobility, we have previously succeeded in demonstrating the regulation of cellular responses, such as cellular adhesion, proliferation, and differentiation. In the present study, we investigated differences in the cellular responses to PRX surfaces versus commercially available tissue culture polystyrene (TCPS) surfaces using fibroblasts, preosteoblasts, and preadipocytes. PRX surfaces were found to more significantly promote cellular proliferation than the TCPS surfaces, regardless of the cell type. To identify the signaling pathways involved in the activation of cellular proliferation, a DNA microarray analysis was performed. PRX surfaces showed a significant increase in the integrin-mediated cell adhesion and focal adhesion pathways. Furthermore, PRX surfaces also promoted osteoblast differentiation more than TCPS. These results suggest that structural features of PRX surfaces act as mechanical cues to dominate cellular proliferation and differentiation.

Cellular responses to novel, micropatterned biomaterials

2008 ◽  
Vol 80 (11) ◽  
pp. 2479-2487 ◽  
Author(s):  
Marga C. Lensen ◽  
Vera A. Schulte ◽  
Jochen Salber ◽  
Mar Diez ◽  
Fabian Menges ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Cellular Responses ◽  
Poly Ethylene Glycol ◽  
Uv Curable ◽  
Cellular Behavior ◽  
Remarkable Effect ◽  
Support Cell ◽  
Poly Ethylene ◽  
Line Patterns ◽  
Perfluorinated Polyether

Two UV-curable polymers, i.e., a star-shaped poly(ethylene glycol) (PEG) and a linear perfluorinated polyether (PFPE), are investigated as novel biomaterials in a systematic study of the cellular responses to surface chemistry, topography, and elasticity. Based on the wettability it was expected that the two novel biomaterials were too hydrophilic or -phobic, respectively, to support cell adhesion. Indeed, no cell adhesion was observed on the smooth, unstructured elastomers, whereas the materials showed no cytotoxicity. However, when the materials bear defined, topographic patterns (prepared by UV-based imprinting), cells do react strongly to the surfaces; they adhere, spread, and change their shape depending on the geometry of the features. Typically, cells were found to align along line patterns and "float" on pillar structures. It should be noted that the chemistry of the surface is not altered by the imprinting process, hence, there are no biofunctional molecules present at the surface to aid the cell adhesion. Finally, a remarkable effect of elasticity on the cellular behavior was discovered. Thus, the three parameters of chemistry, topography, and elasticity were investigated in- and interdependently, and it was found that the biomaterials may lose their resistance to protein adsorption and cell adhesion depending on the surface topography.

Using Mass Spectrometry to Investigate the Structural Features of Photocrosslinked Co-Networks based on Gelatin and Poly(ethylene glycol) Methacrylates

2012 ◽  
Vol 1403 ◽  
Author(s):  
Benjamin F. Pierce ◽  
Axel T. Neffe ◽  
Andreas Lendlein
Keyword(s):  
Mass Spectrometry ◽  
Ethylene Glycol ◽  
Degradation Products ◽  
Structural Features ◽  
Valuable Insight ◽  
Poly Ethylene Glycol ◽  
Buffer Solution ◽  
Molecular Weight Range ◽  
Poly Ethylene

ABSTRACTGelatin was functionalized with glycidyl methacrylate and photocrosslinked in the presence of poly(ethylene glycol) dimethacrylate (PEGDMA) or poly(ethylene glycol) monomethacrylate (PEGMA) to create a biopolymer-based system with tailorable properties. These co-networks were hydrolyzed using 6 M HCl and the degradation products were analyzed and identified using matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry. This technique successfully identified gelatin-derived peptides such as FLPEPPE, SFLPEPPE, and SFLPEPPEE as well as an accompanying PEG-g-poly(methacrylic acid) component. No oligo- or polymethacrylates were monitored at any molecular weight range above m/z = 500, which indicated that they possessed lower molecular weights. An in vitro hydrolytic degradation experiment performed in pH 7.4 PBS buffer solution at 37 °C showed that these networks, which were prepared without the addition of a potentially toxic photoinitiator, exhibited mass loss of up to 50 wt% at 6 weeks of incubation time. These results provide valuable insight into how these functional gelatin-based co-network biomaterials will perform in a biological setting.

scholarly journals Nanofeatured Titanium Surfaces for Dental Implantology: Biological Effects, Biocompatibility, and Safety

2017 ◽  
Vol 2017 ◽  
pp. 1-18 ◽  
Author(s):  
Ruggero Rodriguez y Baena ◽  
Silvana Rizzo ◽  
Luigi Manzo ◽  
Saturnino Marco Lupi
Keyword(s):  
Surface Modification ◽  
Biological Effects ◽  
Bacterial Colonization ◽  
Antibacterial Properties ◽  
Cellular Responses ◽  
Endosseous Implants ◽  
Proliferation And Differentiation ◽  
Titanium Surfaces ◽  
Titanium Substrates

Nanotechnology enables the control and modification of the chemical and topographical characteristics of materials of size less than 100 nm, down to 10 nm. The goal of this review is to discuss the role of titanium substrates as nanoscale surface modification tools for improving various aspects of implantology, including osseointegration and antibacterial properties. Techniques that can impart nanoscale topographical features to endosseous implants are described. Since the advent of nanotechnology, cellular specific functions, such as adhesion, proliferation, and differentiation, have been better understood. By applying these technologies, it is possible to direct cellular responses and improve osseointegration. Conversely, modulating surface features by nanotechnology could have the effect of decreased bacterial colonization.

scholarly journals Transient cellular adhesion on poly(ethylene-glycol)-dimethacrylate hydrogels facilitates a novel stem cell bandage approach

PLoS ONE ◽  
2018 ◽  
Vol 13 (8) ◽  
pp. e0202825 ◽  
Author(s):  
Rosita R. Asawa ◽  
Jessica C. Belkowski ◽  
Daniel A. Schmitt ◽  
Elizabeth M. Hernandez ◽  
Ann E. Babcock ◽  
...  
Keyword(s):  
Stem Cell ◽  
Ethylene Glycol ◽  
Cellular Adhesion ◽  
Ethylene Glycol Dimethacrylate ◽  
Glycol Dimethacrylate ◽  
Poly Ethylene Glycol ◽  
Poly Ethylene

scholarly journals Inside polyMOFs: layered structures in polymer-based metal–organic frameworks

2020 ◽  
Vol 11 (38) ◽  
pp. 10523-10528 ◽  
Author(s):  
Kyle C. Bentz ◽  
Karthikeyan Gnanasekaran ◽  
Jake B. Bailey ◽  
Sergio Ayala ◽  
F. Akif Tezcan ◽  
...  
Keyword(s):  
Ethylene Glycol ◽  
Metal Organic Frameworks ◽  
Layered Structures ◽  
Structural Features ◽  
Equal Mass ◽  
Poly Ethylene Glycol ◽  
Benzenedicarboxylic Acid ◽  
Mass Ratios ◽  
Metal Organic ◽  
Poly Ethylene

In this report, we explore the internal structural features of polyMOFs consisting of equal mass ratios of metal-coordinating poly(benzenedicarboxylic acid) blocks and non-coordinating poly(ethylene glycol) (PEG) blocks.

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