scholarly journals Novel PEEK Copolymer Synthesis and Biosafety – I: Cytotoxicity Evaluation for Clinical Application

Polymers ◽  
2019 ◽  
Vol 11 (11) ◽  
pp. 1803
Author(s):  
Chon ◽  
Yang ◽  
Lee ◽  
Kim ◽  
Jeon ◽  
...  
Keyword(s):  
Thermal Characteristics ◽  
In Vitro Cytotoxicity ◽  
Cytotoxicity Test ◽  
Solution Polymerization ◽  
The Novel ◽  
Test Results ◽  
Diphenyl Sulfone ◽  
Peek Composite ◽  
Polymerization Method

In this research, we synthesized novel polyetheretherketone (PEEK) copolymers and evaluated the biosafety and cytotoxicity of their composites for spinal cage applications in the orthopedic field. The PEEK copolymers and their composites were prepared through a solution polymerization method using diphenyl sulfone as a polymerization solvent. The composite of PEEK copolymer showed good mechanical properties similar to that of natural bone, and also showed good thermal characteristics for the processing of clinical use as spine cage. The results of an in vitro cytotoxicity test did not show any evidence of a toxic effect on the novel PEEK composite. On the basis of these cytotoxicity test results, the PEEK composite also proved its in vitro biosafety for application to an implantable spine cage.

scholarly journals Bioactive Icariin/β-CD-IC/Bacterial Cellulose with Enhanced Biomedical Potential

Nanomaterials ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 387
Author(s):  
Alfred Mensah ◽  
Yajun Chen ◽  
Benjamin K. Asinyo ◽  
Ebenezer Kofi Howard ◽  
Christopher Narh ◽  
...  
Keyword(s):  
Drug Release ◽  
Inclusion Complex ◽  
Bacterial Cellulose ◽  
Antioxidant Properties ◽  
Inclusion Complexation ◽  
Face Mask ◽  
In Vitro Cytotoxicity ◽  
Cytotoxicity Test ◽  
Test Results

A “super” bioactive antibacterial hydrogel, Icariin-β-CD-inclusion complex/Bacterial cellulose and an equally capable counterpart Icariin-Bacterial cellulose (ICBC) were successfully produced with excellent antioxidant properties. The highly porous hydrogels demonstrated very high fluid/liquid absorption capability and were functionally active as Fourier Transform Infrared Spectrometer (FTIR) test confirmed the existence of abundant hydroxyls (-OH stretching), carboxylic acids (-CH2/C-O stretching), Alkyne/nitrile (C≡C/C≡N stretching with triple bonds) and phenol (C-H/N-O symmetric stretching) functional groups. Scanning electron microscope (SEM) and X-ray diffraction (XRD) tests confirmed a successful β-CD-inclusion complexation with Icariin with a great potential for sustained and controlled drug release. In vitro drug release test results indicated a systemic and controlled release of the drug (Icariin) from the internal cavities of the β-CD inclusion complex incorporated inside the BC matrix with high Icariin (drug) release rates. Impressive inactivation rates against Gram-negative bacteria Escherichia coli ATCC 8099 and gram-positive bacteria Staphylococcus aureus ATCC 6538; >99.19% and >98.89% respectively were recorded, as the materials proved to be non-toxic on L929 cells in the in vitro cytotoxicity test results. The materials with promising versatile multipurpose administration of Icariin for wound dressing (as wound dressers), can also be executed as implants for tissue regeneration, as well as face-mask for cosmetic purposes.

scholarly journals Synthesis and Properties of CurNQ for the Theranostic Application in Ovarian Cancer Intervention

Molecules ◽  
2020 ◽  
Vol 25 (19) ◽  
pp. 4471
Author(s):  
Lara G. Freidus ◽  
Pradeep Kumar ◽  
Thashree Marimuthu ◽  
Priyamvada Pradeep ◽  
Viness Pillay ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Cancer Detection ◽  
Ovarian Cancer Cell ◽  
In Vitro Cytotoxicity ◽  
Cancer Targeting ◽  
The Novel ◽  
Cytotoxicity Assays ◽  
Ovarian Cancer Cell Lines ◽  
Cancer Intervention

Synthesis of a novel theranostic molecule for targeted cancer intervention. A reaction between curcumin and lawsone was carried out to yield the novel curcumin naphthoquinone (CurNQ) molecule (2,2′-((((1E,3Z,6E)-3-hydroxy-5-oxohepta-1,3,6-triene-1,7-diyl) bis(2-methoxy-4,1-phenylene))bis(oxy))bis(naphthalene-1,4-dione). CurNQ’s structure was elucidated and was fully characterized. CurNQ was demonstrated to have pH specific solubility, its saturation solubility increased from 11.15 µM at pH 7.4 to 20.7 µM at pH 6.8. This pH responsivity allows for cancer targeting (Warburg effect). Moreover, CurNQ displayed intrinsic fluorescence, thus enabling imaging and detection applications. In vitro cytotoxicity assays demonstrated the chemotherapeutic properties of CurNQ as CurNQ reduced cell viability to below 50% in OVCAR-5 and SKOV3 ovarian cancer cell lines. CurNQ is a novel theranostic molecule for potential targeted cancer detection and treatment.

A Comparison of Ecotoxicological Tests

2002 ◽  
Vol 30 (5) ◽  
pp. 539-550 ◽  
Author(s):  
James L. Botsford
Keyword(s):  
Lethal Dose ◽  
Correlation Coefficients ◽  
Rat Hepatocytes ◽  
In Vitro Cytotoxicity ◽  
Test Results ◽  
Average Correlation ◽  
Ecotoxicological Tests ◽  
Present Test ◽  
Lethal Doses

A simple, inexpensive and rapid method of determining toxicity by using a bacterium as the indicator organism was developed and compared with 23 other tests. The average correlation coefficient when comparing these 23 tests with the present test was 0.800, ranging from 0.580 to 0.950. Eleven of the tests were compared in detail by using 35 of the chemicals on the Multicentre Evaluation of In Vitro Cytotoxicity list of test chemicals. Comparing results from the present test with test results for these 35 chemicals with Microtox™, Biotox™, Daphnia magna, rat hepatocytes and ascites tumour cell resulted in correlation coefficients ranging from 0.871 to 0.933. Comparisons of the test data with rodent LD50 values, human lethal dose estimates from autopsies and human lethal doses obtained from the literature provided correlation coefficients ranging from 0.580 to 0.770, indicating that the test compares less favourably with these methods. This test provides data comparable to data from other ecotoxicological tests.

Cytocompatibility Comparative Study of Hydroxyapatite Coating on Titanium Alloy Treated with Two Chemical Routes

2013 ◽  
Vol 658 ◽  
pp. 61-66
Author(s):  
Qing Zong Si ◽  
Xiao Li An ◽  
Yang Li ◽  
Bin Liu ◽  
Jin Qing Wang
Keyword(s):  
Titanium Alloy ◽  
In Vitro Cytotoxicity ◽  
Combination Method ◽  
Cytotoxicity Test ◽  
X Ray Diffraction ◽  
Chemical Methods ◽  
X Ray ◽  
Ha Coating ◽  
Cell Adhesion And Proliferation

In order to prepare bioactive hydroxyapatite (Ca10 (PO4)6(OH) 2, HA) coating with ideal biocompatibility, the surface of titanium alloy was treated with the two kinds of chemical methods, which are the acid-alkali-combination method and Self-polymerization-adhesion of dopamine. After pretreatment, the treated titanium alloy plates were immersed in simulated body fluid (SBF) to form HA coating on their surface. The chemical composition of the coating was analyzed by an X-ray diffraction (XRD) and the morphology was observed by a scanning electron microscope (SEM). After that, the plates were training in vitro cytotoxicity test with MC3T3-E1 osteoblasts. Compared with the results of cell culture, the method of Self- polymerization -adhesion of dopamine showed better cell adhesion and proliferation..

scholarly journals Study of Icaritin Films by Low-Energy Electron Beam Deposition

2021 ◽  
Vol 23 (2) ◽  
pp. 77
Author(s):  
Tongfei Cheng ◽  
Jinxing Cao ◽  
Xiaohong Jiang ◽  
M.A. Yarmolenko ◽  
A.A. Rogachev ◽  
...  
Keyword(s):  
Electron Beam ◽  
In Vitro Cytotoxicity ◽  
Low Energy ◽  
Cytotoxicity Test ◽  
Soaking Time ◽  
Energy Beam ◽  
Electron Beam Deposition ◽  
Relative Cell Viability ◽  
Beam Deposition

In this paper, icaritin film was prepared by low-energy beam electron beam deposition (EBD). The material test showed that the structure and composition of icaritin were not changed after electron beam deposition. Then, the film was sliced and immersed in simulated body fluids, it can be seen that the film was released quickly in the first 7 days. With the extension of soaking time, the release rate gradually slowed down, and the release amount exceeded 90% in about 20 days. In vitro cytotoxicity test showed that the relative cell viability rate of the film was still 92.32±1.30% (p<0.05), indicating that the film possessed excellent cytocompatibility.

scholarly journals The properties of MOF-Zn2(EBNB)2(BPY)2·2H2O and its basic study of loading Methadone

2020 ◽  
Author(s):  
Deng Linxin ◽  
Li Song ◽  
Xuehua Sun
Keyword(s):  
Drug Loading ◽  
In Vitro Release ◽  
In Vitro Cytotoxicity ◽  
Cytotoxicity Test ◽  
Two Phase ◽  
Basic Study ◽  
Nitrobenzoic Acid ◽  
Model Drug ◽  
Loading Amount

Abstract The ligands of (E)-bis(p-3-nitrobenzoic acid) vinyl (C16H10N2O8) were synthesized in three steps, and then the MOF-Zn2(EBNB)2(BPY)2·2H2O was synthesized by solvothermal method. This structure was characterized by X-ray single crystal diffraction, SEM and TG. The drug loading and in vitro release of Zn2(EBNB)2(BPY)2·2H2O were also studied with Methadone as model drug. The result shows that the highest loading amount of Zn2(EBNB)2(BPY)2·2H2O to Methadone was 0.256g/g, and the drug delivery system was a two-phase mode. The results of in vitro cytotoxicity test shows that Zn2(EBNB)2(BPY)2·2H2O has good biocompatibility.

Novel rapid in vitro cytotoxicity test on mammalian cells based on an electrochemical measuring method

2014 ◽  
Vol 44 (8) ◽  
pp. 935-943 ◽  
Author(s):  
Michael Pescheck ◽  
Constanze Dürr ◽  
Luděk Bláha ◽  
Dieter Sell
Keyword(s):  
Mammalian Cells ◽  
Measuring Method ◽  
In Vitro Cytotoxicity ◽  
Cytotoxicity Test
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