scholarly journals Effects of surface-modifying ligands on the colloidal stability of ZnO nanoparticle dispersions in in vitro cytotoxicity test media

2014 ◽  
pp. 57 ◽  
Author(s):  
Seong Soo A. An ◽  
Dongwook Kwon ◽  
Jaehong Park ◽  
Seo Yeon Choi ◽  
Tae Hyun Yoon ◽  
...  
Keyword(s):  
Colloidal Stability ◽  
In Vitro Cytotoxicity ◽  
Cytotoxicity Test ◽  
Zno Nanoparticle ◽  
Nanoparticle Dispersions

Cytocompatibility Comparative Study of Hydroxyapatite Coating on Titanium Alloy Treated with Two Chemical Routes

2013 ◽  
Vol 658 ◽  
pp. 61-66
Author(s):  
Qing Zong Si ◽  
Xiao Li An ◽  
Yang Li ◽  
Bin Liu ◽  
Jin Qing Wang
Keyword(s):  
Titanium Alloy ◽  
In Vitro Cytotoxicity ◽  
Combination Method ◽  
Cytotoxicity Test ◽  
X Ray Diffraction ◽  
Chemical Methods ◽  
X Ray ◽  
Ha Coating ◽  
Cell Adhesion And Proliferation

In order to prepare bioactive hydroxyapatite (Ca10 (PO4)6(OH) 2, HA) coating with ideal biocompatibility, the surface of titanium alloy was treated with the two kinds of chemical methods, which are the acid-alkali-combination method and Self-polymerization-adhesion of dopamine. After pretreatment, the treated titanium alloy plates were immersed in simulated body fluid (SBF) to form HA coating on their surface. The chemical composition of the coating was analyzed by an X-ray diffraction (XRD) and the morphology was observed by a scanning electron microscope (SEM). After that, the plates were training in vitro cytotoxicity test with MC3T3-E1 osteoblasts. Compared with the results of cell culture, the method of Self- polymerization -adhesion of dopamine showed better cell adhesion and proliferation..

scholarly journals Study of Icaritin Films by Low-Energy Electron Beam Deposition

2021 ◽  
Vol 23 (2) ◽  
pp. 77
Author(s):  
Tongfei Cheng ◽  
Jinxing Cao ◽  
Xiaohong Jiang ◽  
M.A. Yarmolenko ◽  
A.A. Rogachev ◽  
...  
Keyword(s):  
Electron Beam ◽  
In Vitro Cytotoxicity ◽  
Low Energy ◽  
Cytotoxicity Test ◽  
Soaking Time ◽  
Energy Beam ◽  
Electron Beam Deposition ◽  
Relative Cell Viability ◽  
Beam Deposition

In this paper, icaritin film was prepared by low-energy beam electron beam deposition (EBD). The material test showed that the structure and composition of icaritin were not changed after electron beam deposition. Then, the film was sliced and immersed in simulated body fluids, it can be seen that the film was released quickly in the first 7 days. With the extension of soaking time, the release rate gradually slowed down, and the release amount exceeded 90% in about 20 days. In vitro cytotoxicity test showed that the relative cell viability rate of the film was still 92.32±1.30% (p<0.05), indicating that the film possessed excellent cytocompatibility.

scholarly journals The properties of MOF-Zn2(EBNB)2(BPY)2·2H2O and its basic study of loading Methadone

2020 ◽  
Author(s):  
Deng Linxin ◽  
Li Song ◽  
Xuehua Sun
Keyword(s):  
Drug Loading ◽  
In Vitro Release ◽  
In Vitro Cytotoxicity ◽  
Cytotoxicity Test ◽  
Two Phase ◽  
Basic Study ◽  
Nitrobenzoic Acid ◽  
Model Drug ◽  
Loading Amount

Abstract The ligands of (E)-bis(p-3-nitrobenzoic acid) vinyl (C16H10N2O8) were synthesized in three steps, and then the MOF-Zn2(EBNB)2(BPY)2·2H2O was synthesized by solvothermal method. This structure was characterized by X-ray single crystal diffraction, SEM and TG. The drug loading and in vitro release of Zn2(EBNB)2(BPY)2·2H2O were also studied with Methadone as model drug. The result shows that the highest loading amount of Zn2(EBNB)2(BPY)2·2H2O to Methadone was 0.256g/g, and the drug delivery system was a two-phase mode. The results of in vitro cytotoxicity test shows that Zn2(EBNB)2(BPY)2·2H2O has good biocompatibility.

scholarly journals Solid Lipid Nanoparticles for Dibucaine Sustained Release

Pharmaceutics ◽  
2018 ◽  
Vol 10 (4) ◽  
pp. 231 ◽  
Author(s):  
Raquel de M. Barbosa ◽  
Ligia Ribeiro ◽  
Bruna Casadei ◽  
Camila da Silva ◽  
Viviane Queiróz ◽  
...  
Keyword(s):  
Solid Lipid Nanoparticles ◽  
Encapsulation Efficiency ◽  
Colloidal Stability ◽  
Lipid Nanoparticles ◽  
In Vitro Cytotoxicity ◽  
Tail Flick ◽  
Release Kinetic ◽  
Solid Lipid

Dibucaine (DBC) is among the more potent long-acting local anesthetics (LA), and it is also one of the most toxic. Over the last decades, solid lipid nanoparticles (SLN) have been developed as promising carriers for drug delivery. In this study, SLN formulations were prepared with the aim of prolonging DBC release and reducing its toxicity. To this end, SLN composed of two different lipid matrices and prepared by two different hot-emulsion techniques (high-pressure procedure and sonication) were compared. The colloidal stability of the SLN formulations was tracked in terms of particle size (nm), polydispersity index (PDI), and zeta potential (mV) for 240 days at 4 °C; the DBC encapsulation efficiency was determined by the ultrafiltration/centrifugation method. The formulations were characterized by differential scanning calorimetry (DSC), electron paramagnetic resonance (EPR), and release kinetic experiments. Finally, the in vitro cytotoxicity against 3T3 fibroblast and HaCaT cells was determined, and the in vivo analgesic action was assessed using the tail flick test in rats. Both of the homogenization procedures were found suitable to produce particles in the 200 nm range, with good shelf stability (240 days) and high DBC encapsulation efficiency (~72–89%). DSC results disclosed structural information on the nanoparticles, such as the lower crystallinity of the lipid core vs. the bulk lipid. EPR measurements provided evidence of DBC partitioning in both SLNs. In vitro (cytotoxicity) and in vivo (tail flick) experiments revealed that the encapsulation of DBC into nanoparticles reduces its intrinsic cytotoxicity and prolongs the anesthetic effect, respectively. These results show that the SLNs produced are safe and have great potential to extend the applications of dibucaine by enhancing its bioavailability.

Novel rapid in vitro cytotoxicity test on mammalian cells based on an electrochemical measuring method

2014 ◽  
Vol 44 (8) ◽  
pp. 935-943 ◽  
Author(s):  
Michael Pescheck ◽  
Constanze Dürr ◽  
Luděk Bláha ◽  
Dieter Sell
Keyword(s):  
Mammalian Cells ◽  
Measuring Method ◽  
In Vitro Cytotoxicity ◽  
Cytotoxicity Test

Research on the Preparation of HA/PEEK Gradient Composites by Impregnation Method and the Biosecurity

2017 ◽  
Vol 893 ◽  
pp. 35-42 ◽  
Author(s):  
Rui Ma ◽  
Ling Jun Dai ◽  
Lin Wang ◽  
Yu Dian Song
Keyword(s):  
Mechanical Properties ◽  
Bone Formation ◽  
Structural Design ◽  
Injection Moulding ◽  
In Vitro Cytotoxicity ◽  
Leach Liquor ◽  
Cytotoxicity Test ◽  
Impregnation Method ◽  
The Poor

This study prepares the HA/PEEK gradient composites with special structural design by impregnation method, which eases the contradiction of the HA/PEEK composites between the lack of bioactivity due to low HA contents and the poor mechanical properties due to high HA contents, and effectively improves the bioactivity and mechanical properties of the PEEK-based materials, and then evaluates the biosecurity of HA/PEEK gradient composites. The main functional cell of bone formation—osteoblast is adopted for the co-culture with PEEK, HA/PEEK gradient composites and the leach liquor, and the effects of PEEK and HA/PEEK gradient composites on the proliferative functions of osteoblast are studied by in vitro cytotoxicity test. The results show that no harmful admixtures are produced in the process of injection moulding, and the leach liquors of PEEK and HA/PEEK gradient composites have no effects on the proliferative functions of osteoblast, with good osteoblast compatibility.

SYNTHESIS OF PEG-ENCAPSULATED SUPERPARAMAGNETIC COLLOIDAL NANOCRYSTALS CLUSTERS

NANO ◽  
2010 ◽  
Vol 05 (06) ◽  
pp. 333-339 ◽  
Author(s):  
HUI WANG ◽  
YIMING LI ◽  
ZHAOFENG LUO ◽  
SHUAI ZHOU ◽  
JIN SHENG ◽  
...  
Keyword(s):  
Cell Viability ◽  
Colloidal Stability ◽  
Magnetic Measurements ◽  
Cluster Structure ◽  
Surface Group ◽  
In Vitro Cytotoxicity ◽  
Transmission Electron ◽  
Small Primary ◽  
Major Surface

PEG-encapsulated colloidal nanocrystal clusters (CNCs) have been synthesized via a one-step solvothermal process at a temperature of 230°C. The composition, phase, and morphology of these CNCs have been characterized by X-ray diffraction and transmission electron microscopy. Studies show that each particle is a cluster structure consisting of small primary iron oxide nanocrystals. Magnetic measurements reveal the superparamagnetic nature of these CNCs at room temperature. The CNCs with different sizes (80 nm or 95 nm) can be obtained by changing the time of reaction. The dispersibility and colloidal stability of these CNCs with PEG as the major surface group have also been discussed. In vitro cytotoxicity of these CNCs with different thickness PEG layer on HeLa cell has also been assayed. Cytotoxicity results reveal that the CNCs concentration and the incubation time can influence the cell viability, and the size of CNCs almost does not affect the cell viability.

scholarly journals Novel PEEK Copolymer Synthesis and Biosafety – I: Cytotoxicity Evaluation for Clinical Application

Polymers ◽  
2019 ◽  
Vol 11 (11) ◽  
pp. 1803
Author(s):  
Chon ◽  
Yang ◽  
Lee ◽  
Kim ◽  
Jeon ◽  
...  
Keyword(s):  
Thermal Characteristics ◽  
In Vitro Cytotoxicity ◽  
Cytotoxicity Test ◽  
Solution Polymerization ◽  
The Novel ◽  
Test Results ◽  
Diphenyl Sulfone ◽  
Peek Composite ◽  
Polymerization Method

In this research, we synthesized novel polyetheretherketone (PEEK) copolymers and evaluated the biosafety and cytotoxicity of their composites for spinal cage applications in the orthopedic field. The PEEK copolymers and their composites were prepared through a solution polymerization method using diphenyl sulfone as a polymerization solvent. The composite of PEEK copolymer showed good mechanical properties similar to that of natural bone, and also showed good thermal characteristics for the processing of clinical use as spine cage. The results of an in vitro cytotoxicity test did not show any evidence of a toxic effect on the novel PEEK composite. On the basis of these cytotoxicity test results, the PEEK composite also proved its in vitro biosafety for application to an implantable spine cage.

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