scholarly journals Application of DNA Quadruplex Hydrogels Prepared from Polyethylene Glycol-Oligodeoxynucleotide Conjugates to Cell Culture Media

Polymers ◽  
2019 ◽  
Vol 11 (10) ◽  
pp. 1607
Author(s):  
Shizuma Tanaka ◽  
Shinsuke Yukami ◽  
Yuhei Hachiro ◽  
Yuichi Ohya ◽  
Akinori Kuzuya
Keyword(s):  
Cell Culture ◽  
High Efficiency ◽  
Culture Media ◽  
Culture Conditions ◽  
Cell Culture Media ◽  
G Quadruplex ◽  
L929 Fibroblast ◽  
Dna Quadruplex ◽  
Quadruplex Formation ◽  
Poly Ethylene

Application of Na+-responsive DNA quadruplex hydrogels, which utilize G-quadruplexes as crosslinking points of poly(ethylene glycol) (PEG) network as cell culture substrate, has been examined. PEG-oligodeoxynucleotide (ODN) conjugate, in which four deoxyguanosine (dG4) residues are tethered to both ends of PEG, was prepared by modified high-efficiency liquid phase (HELP) synthesis of oligonucleotides and used as the macromonomer. When mixed with equal volume of cell culture media, the solution of PEG-ODN turned into stiff hydrogel (G-quadruplex hydrogel) as the result of G-quadruplex formation by the dG4 segments in the presence of Na+. PEG-ODN itself did not show cytotoxicity and the resulting hydrogel was stable enough under cell culture conditions. However, L929 fibroblast cells cultured in G-quadruplex hydrogel remained spherical for a week, yet alive, without proliferation. The cells gradually sedimented through the gel day by day, probably due to the reversible nature of G-quadruplex formation and the resulting slow rearrangement of the macromonomers. Once they reached the bottom glass surface, the cells started to spread and proliferate.

scholarly journals Expression of Human Erythropoietin Containing 2 Additional N-Link in CHO-K1 Cells under Different Culture Conditions

2019 ◽  
Vol 10 (1) ◽  
pp. 7
Author(s):  
Adi Santoso ◽  
Larasati Larasati ◽  
Arizah Kusumawati ◽  
Popi Hadi Wisnuwardhani ◽  
Ratih Asma Ningrum ◽  
...  
Keyword(s):  
Cell Culture ◽  
Protein Expression ◽  
Culture Media ◽  
Fetal Bovine Serum ◽  
Culture Conditions ◽  
Expression Level ◽  
Seeding Density ◽  
Cell Culture Media ◽  
Human Erythropoietin ◽  
Fetal Bovine

Human erythropoietin (hEPO) is a glycoprotein that regulates the formation of erythrocytes and mainly used in anemia patients. Previously, we have reported the expression of modified human EPO with 2 additional N-linked in mammalian cell CHO-K1. The aim of this current research was to study the optimum condition for modified recombinant hEPO (rhEPO) production in CHO-K1. To do this, several parameters of culture conditions were applied including antibiotic concentrations, seeding densities, time of incubations, fetal bovine serum (FBS) concentrations and cell culture media. The result showed that the presence of antibiotic G418 improved the expression level with the highest was at 1% of concentration. Meanwhile, seeding density of 2–3x105 cells/6 cm dish and seven day of incubation time were the best condition for rhEPO protein expression. From five different combination media used, F12 medium with 10% FBS gave the highest expression of rhEPO protein. From this study was also found that at passage 16 the expression level was still increasing proving that the clone expressing the protein of our interest is promisingly stable.Keywords : EPO, erythropoietin, protein expression, CHO-K1, optimation

scholarly journals The Cell Culture Medium Affects Growth, Phenotype Expression and the Response to Selenium Cytotoxicity in A549 and HepG2 Cells

Antioxidants ◽  
2019 ◽  
Vol 8 (5) ◽  
pp. 130 ◽  
Author(s):  
Lisa Arodin Selenius ◽  
Marita Wallenberg Lundgren ◽  
Rim Jawad ◽  
Olof Danielsson ◽  
Mikael Björnstedt
Keyword(s):  
Cell Culture ◽  
Cell Growth ◽  
Culture Medium ◽  
Culture Media ◽  
A549 Cells ◽  
Culture Conditions ◽  
Cell Culture Medium ◽  
Cell Culture Media ◽  
Toxic Response ◽  
Selenium Compounds

Selenium compounds influence cell growth and are highly interesting candidate compounds for cancer chemotherapy. Over decades an extensive number of publications have reported highly efficient growth inhibitory effects with a number of suggested mechanisms f especially for redox-active selenium compounds. However, the studies are difficult to compare due to a high degree of variations in half-maximal inhibitor concentration (IC50) dependent on cultivation conditions and methods to assess cell viability. Among other factors, the variability in culture conditions may affect the experimental outcome. To address this, we have compared the maintenance effects of four commonly used cell culture media on two cell lines, A549 and HepG2, evaluated by the toxic response to selenite and seleno-methylselenocysteine, cell growth and redox homeostasis. We found that the composition of the cell culture media greatly affected cell growth and sensitivity to selenium cytotoxicity. We also provided evidence for change of phenotype in A549 cells when maintained under different culture conditions, demonstrated by changes in cytokeratin 18 (CK18) and vimentin expression. In conclusion, our results have shown the importance of defining the cell culture medium used when comparing results from different studies.

Cell culture media: NMR approaches to evaluating quality and nutrient consumption

Planta Medica ◽  
2016 ◽  
Vol 81 (S 01) ◽  
pp. S1-S381
Author(s):  
KB Killday ◽  
AS Freund ◽  
C Fischer ◽  
KL Colson
Keyword(s):  
Cell Culture ◽  
Culture Media ◽  
Cell Culture Media ◽  
Nutrient Consumption

Selection and preliminary application of DNA aptamer targeting A549 excreta in cell culture media

2021 ◽  
pp. 106811
Author(s):  
Yuanbin Guo ◽  
Ming Shi ◽  
Xiujuan Liu ◽  
Huagang Liang ◽  
Liming Gao ◽  
...  
Keyword(s):  
Cell Culture ◽  
Culture Media ◽  
Dna Aptamer ◽  
Cell Culture Media ◽  
Preliminary Application

scholarly journals Benchmarking of commercially available CHO cell culture media for antibody production

2015 ◽  
Vol 99 (11) ◽  
pp. 4645-4657 ◽  
Author(s):  
David Reinhart ◽  
Lukas Damjanovic ◽  
Christian Kaisermayer ◽  
Renate Kunert
Keyword(s):  
Cell Culture ◽  
Antibody Production ◽  
Culture Media ◽  
Cho Cell ◽  
Cell Culture Media ◽  
Cho Cell Culture

scholarly journals Intestinal and Hepatic Uptake of Dietary Peroxidized Lipids and Their Decomposition Products, and Their Subsequent Effects on Apolipoprotein A1 and Paraoxonase1

Antioxidants ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 1258
Author(s):  
Xueting Jiang ◽  
Pragney Deme ◽  
Rajat Gupta ◽  
Dmitry Litvinov ◽  
Kathryn Burge ◽  
...  
Keyword(s):  
Cell Culture ◽  
Culture Media ◽  
Degradation Products ◽  
Apolipoprotein A1 ◽  
Paraoxonase 1 ◽  
Pon1 Activity ◽  
Atherosclerotic Cardiovascular Disease ◽  
Metabolic Fate ◽  
Cell Culture Media ◽  
Decomposition Products

Both pro- and antiatherosclerotic effects have been ascribed to dietary peroxidized lipids. Confusion on the role of peroxidized lipids in atherosclerotic cardiovascular disease is punctuated by a lack of understanding regarding the metabolic fate and potential physiological effects of dietary peroxidized lipids and their decomposition products. This study sought to determine the metabolic fate and physiological ramifications of 13-hydroperoxyoctadecadienoic acid (13-HPODE) and 13-HODE (13-hydroxyoctadecadienoic acid) supplementation in intestinal and hepatic cell lines, as well as any effects resulting from 13-HPODE or 13-HODE degradation products. In the presence of Caco-2 cells, 13-HPODE was rapidly reduced to 13-HODE. Upon entering the cell, 13-HODE appears to undergo decomposition, followed by esterification. Moreover, 13-HPODE undergoes autodecomposition to produce aldehydes such as 9-oxononanoic acid (9-ONA). Results indicate that 9-ONA was oxidized to azelaic acid (AzA) rapidly in cell culture media, but AzA was poorly absorbed by intestinal cells and remained detectable in cell culture media for up to 18 h. An increased apolipoprotein A1 (ApoA1) secretion was observed in Caco-2 cells in the presence of 13-HPODE, 9-ONA, and AzA, whereas such induction was not observed in HepG2 cells. However, 13-HPODE treatments suppressed paraoxonase 1 (PON1) activity, suggesting the induction of ApoA1 secretion by 13-HPODE may not represent functional high-density lipoprotein (HDL) capable of reducing oxidative stress. Alternatively, AzA induced both ApoA1 secretion and PON1 activity while suppressing ApoB secretion in differentiated Caco-2 cells but not in HepG2. These results suggest oxidation of 9-ONA to AzA might be an important phenomenon, resulting in the accumulation of potentially beneficial dietary peroxidized lipid-derived aldehydes.

Characterization of the impact of classical cell‐culture media on the response of electrochemical sensors

2021 ◽  
Author(s):  
Ayman Chmayssem ◽  
Lauriane Petit ◽  
Nicolas Verplanck ◽  
Véronique Mourier ◽  
Séverine Vignoud ◽  
...  
Keyword(s):  
Cell Culture ◽  
Electrochemical Sensors ◽  
Culture Media ◽  
Cell Culture Media ◽  
The Impact
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