scholarly journals The Cell Culture Medium Affects Growth, Phenotype Expression and the Response to Selenium Cytotoxicity in A549 and HepG2 Cells

Antioxidants ◽  
2019 ◽  
Vol 8 (5) ◽  
pp. 130 ◽  
Author(s):  
Lisa Arodin Selenius ◽  
Marita Wallenberg Lundgren ◽  
Rim Jawad ◽  
Olof Danielsson ◽  
Mikael Björnstedt
Keyword(s):  
Cell Culture ◽  
Cell Growth ◽  
Culture Medium ◽  
Culture Media ◽  
A549 Cells ◽  
Culture Conditions ◽  
Cell Culture Medium ◽  
Cell Culture Media ◽  
Toxic Response ◽  
Selenium Compounds

Selenium compounds influence cell growth and are highly interesting candidate compounds for cancer chemotherapy. Over decades an extensive number of publications have reported highly efficient growth inhibitory effects with a number of suggested mechanisms f especially for redox-active selenium compounds. However, the studies are difficult to compare due to a high degree of variations in half-maximal inhibitor concentration (IC50) dependent on cultivation conditions and methods to assess cell viability. Among other factors, the variability in culture conditions may affect the experimental outcome. To address this, we have compared the maintenance effects of four commonly used cell culture media on two cell lines, A549 and HepG2, evaluated by the toxic response to selenite and seleno-methylselenocysteine, cell growth and redox homeostasis. We found that the composition of the cell culture media greatly affected cell growth and sensitivity to selenium cytotoxicity. We also provided evidence for change of phenotype in A549 cells when maintained under different culture conditions, demonstrated by changes in cytokeratin 18 (CK18) and vimentin expression. In conclusion, our results have shown the importance of defining the cell culture medium used when comparing results from different studies.

scholarly journals Selectivity of direct plasma treatment and plasma-conditioned media in bone cancer cell lines

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Inès Hamouda ◽  
Cédric Labay ◽  
Uroš Cvelbar ◽  
Maria-Pau Ginebra ◽  
Cristina Canal
Keyword(s):  
Cell Culture ◽  
Plasma Treatment ◽  
Cancer Cell ◽  
Culture Medium ◽  
Culture Media ◽  
Bone Cancer ◽  
Reactive Species ◽  
Conditioned Media ◽  
Cell Culture Medium ◽  
Cell Culture Media

AbstractAtmospheric pressure plasma jets have been shown to impact several cancer cell lines, both in vitro and in vivo. These effects are based on the biochemistry of the reactive oxygen and nitrogen species generated by plasmas in physiological liquids, referred to as plasma-conditioned liquids. Plasma-conditioned media are efficient in the generation of reactive species, inducing selective cancer cell death. However, the concentration of reactive species generated by plasma in the cell culture media of different cell types can be highly variable, complicating the ability to draw precise conclusions due to the differential sensitivity of different cells to reactive species. Here, we compared the effects of direct and indirect plasma treatment on non-malignant bone cells (hOBs and hMSCs) and bone cancer cells (SaOs-2s and MG63s) by treating the cells directly or exposing them to previously treated cell culture medium. Biological effects were correlated with the concentrations of reactive species generated in the liquid. A linear increase in reactive species in the cell culture medium was observed with increased plasma treatment time independent of the volume treated. Values up to 700 µM for H2O2 and 140 µM of NO2− were attained in 2 mL after 15 min of plasma treatment in AdvDMEM cell culture media. Selectivity towards bone cancer cells was observed after both direct and indirect plasma treatments, leading to a decrease in bone cancer cell viability at 72 h to 30% for the longest plasma treatment times while maintaining the survival of non-malignant cells. Therefore, plasma-conditioned media may represent the basis for a potentially novel non-invasive technique for bone cancer therapy.

The biochemical effects of extracellular Zn2+and other metal ions are severely affected by their speciation in cell culture media

Metallomics ◽  
2015 ◽  
Vol 7 (1) ◽  
pp. 102-111 ◽  
Author(s):  
H. Haase ◽  
S. Hebel ◽  
G. Engelhardt ◽  
L. Rink
Keyword(s):  
Cell Culture ◽  
Bovine Serum Albumin ◽  
Culture Medium ◽  
Culture Media ◽  
Zinc Homeostasis ◽  
Cell Culture Medium ◽  
Cell Culture Media ◽  
Biochemical Effects ◽  
Cellular Environment

Differential speciation and lower zinc buffering by less bovine serum albumin (BSA) in cell culture medium lead to altered zinc homeostasis compared to the cellular environmentin vivo.

scholarly journals Serum-free medium increases the replication rate of the avian coronavirus infectious bronchitis virus in chicken embryo kidney cells

2021 ◽  
Author(s):  
José A. Quinteros ◽  
Glenn F. Browning ◽  
Amir H. Noormohammadi ◽  
Mark A. Stevenson ◽  
Mauricio J. C. Coppo ◽  
...  
Keyword(s):  
Cell Culture ◽  
Cell Cultures ◽  
Infectious Bronchitis Virus ◽  
Chicken Embryo ◽  
Culture Medium ◽  
Culture Media ◽  
Cell Culture Medium ◽  
Cell Culture Media ◽  
Infectious Bronchitis ◽  
Porcine Epidemic Diarrhoea Virus

AbstractInfectious bronchitis virus (IBV), an avian coronavirus, can be isolated and cultured in tracheal organ cultures (TOCs), embryonated eggs and cell cultures. TOCs and embryonated eggs are commonly used for viral isolation but use of these is laborious and expensive. Cell cultures have been used only with IBV strains that have previously been adapted to grow under laboratory conditions, and not for primary isolation. Previous studies using the coronavirus porcine epidemic diarrhoea virus (PEDV) have suggested that foetal bovine serum (FBS), a common component of cell culture media, can inhibit the adsorption of coronaviruses onto the host cell membrane receptors. In the present study, the replication of IBV in primary chicken embryo kidney (CEK) cell cultures and the Leghorn hepatocellular carcinoma (LMH) cell line was examined using two different cell culture media, one containing FBS and the other containing yeast extract (YE). A reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) assay was used to quantify viral RNA copies in cell lysates. The highest concentrations of viral genomes were observed when the cell culture medium did not contain FBS. Examination of the infectivity of virus grown in CEK cell cultures was examined by titration in embryonated chicken eggs, demonstrating that the cell lysate from CEK cell cultures in medium without FBS contained a higher median embryo infectious dose (EID50) than that from CEK cell cultures in medium containing FBS. These results suggest that improved replication of IBV in cell cultures can be achieved by the omission of FBS from the cell culture medium. This may enhance the potential for production of vaccines in cell culture and facilitate the isolation of emergent IBV strains in cell cultures.

Effect of Different Cell Culture Medium Surfactants on Cell Growth and Viability

2010 ◽  
pp. 819-822 ◽  
Author(s):  
Kristina Martinelle ◽  
Annika Mattsson ◽  
Brita Rippner-Blomqvist ◽  
Elisabeth Lindner
Keyword(s):  
Cell Culture ◽  
Cell Growth ◽  
Culture Medium ◽  
Cell Culture Medium ◽  
Cell Growth And Viability

Using 3D perfusion bioreactor system to studying cell biology of ovarian cancer.

2021 ◽  
Vol 39 (15_suppl) ◽  
pp. e17558-e17558
Author(s):  
Alba Martínez ◽  
Molly Buckley ◽  
Joel Berry ◽  
Rebecca Christian Arend
Keyword(s):  
Ovarian Cancer ◽  
Cell Culture ◽  
Cell Growth ◽  
Cell Lines ◽  
Culture Media ◽  
Tumor Biology ◽  
Perfusion Bioreactor ◽  
Suitable Model ◽  
Cell Culture Media ◽  
Bioreactor System

e17558 Background: Epithelial Ovarian Cancer (EOC) is the most common cause of death among gynecological malignancies. This is a result of the high rate of recurrence and chemo-resistance in EOC patients. Therefore, the development of new therapeutics is crucial. A major factor contributing to this is the lack of therapeutic candidates is lack of translational accuracy in preclinical models. Recently, 3-dimensional (3-D) models have aided in accurately recreating tumor biology. We have developed an EOC 3-D perfused bioreactor system that recapitulates EOC tumor biology and incorporates tumor biomechanical regulation. This model allows for us to more accurately predict the clinical response of new drug candidates, which aids in elimination of ineffective candidates prior to clinical trials. Methods: EOC cell lines (luciferase-taggedSKOV-3 and OVCAR-8) were embedded in a relevant extracellular matrix (ECM) and injected into a perfused, polydimethylsiloxane (PDMS) bioreactor. Microchannels were embedded in matrigel so that the cell culture media with or without chemotherapy could flow through the perfused PDMS to provide nutrient delivery and gas exchange enhancing viability and function of surrounding cells. The bioreactors were connected to a peristaltic pump that allowed for the cell culture media to perfuse over a 7-day period. We monitored cell viability using bioluminescence imaging (BLI), immunohistochemistry (IHC), and lactate dehydrogenase (LDH) release in media. Results: BLI showed a linear increase in SKOV-3 and OVCAR-8 cell growth over 7 days. These results were confirmed by IHC measuring the number of nucleated cells per micron2. Graphical representation of the region of interest (ROI) showed a high correlation between IHC staining of nucleated cells and BLI score. IHC analysis of PAX8 staining was positive and proved that the perfusion bioreactor system maintains EOC biology over time. In addition, our results suggest that the bioreactor is a suitable model for drug preclinical testing in both cell lines as well as in patients’ samples. Conclusions: Our preliminary results using the 3D EOC perfused, PDMS bioreactor model showed increased EOC cell growth overtime, while maintaining original EOC histology. Moreover, our results suggest that this model could provide a novel platform to study therapeutic interventions in EOC. Our ultimate goal is to implement ovarian cancer microenvironment components (e.g. immune cells) into bioreactor system to study different drug treatments to better determine drug candidate’s translational efficacy.

scholarly journals Silk fibroin induces chondrogenic differentiation of canine adipose–derived multipotent mesenchymal stromal cells/mesenchymal stem cells

2019 ◽  
Vol 10 ◽  
pp. 204173141983505 ◽  
Author(s):  
Metka Voga ◽  
Natasa Drnovsek ◽  
Sasa Novak ◽  
Gregor Majdic
Keyword(s):  
Stem Cells ◽  
Cell Culture ◽  
Stromal Cells ◽  
Chondrogenic Differentiation ◽  
Culture Medium ◽  
Collagen Type ◽  
Multipotent Mesenchymal Stromal Cells ◽  
Culture Conditions ◽  
Standard Cell ◽  
Cell Culture Medium

Under appropriate culture conditions, mesenchymal stem cells (MSC), also called more properly multipotent mesenchymal stromal cells (MMSC), can be induced toward differentiation into different cell lineages. In order to guide stem cell fate within an environment resembling the stem cell niche, different biomaterials are being developed. In the present study, we used silk fibroin (SF) as a biomaterial supporting the growth of MMSC and studied its effect on chondrogenesis of canine adipose–derived MMSC (cADMMSC). Adipose tissue was collected from nine privately owned dogs. MMSC were cultured on SF films and SF scaffolds in a standard cell culture medium. Cell morphology was evaluated by scanning electron microscopy (SEM). Chondrogenic differentiation was evaluated by alcian blue staining and mRNA expression of collagen type 1, collagen type 2, Sox9, and Aggrecan genes. cADMMSC cultured on SF films and SF scaffolds stained positive using alcian blue. SEM images revealed nodule-like structures with matrix vesicles and fibers resembling chondrogenic nodules. Gene expression of chondrogenic markers Sox9 and Aggrecan were statistically significantly upregulated in cADMMSC cultured on SF films in comparison to negative control cADMMSC. This result suggests that chondrogenesis of cADMMSC could occur when cells were grown on SF films in a standard cell culture medium without specific culture conditions, which were previously considered necessary for induction of chondrogenic differentiation.

scholarly journals Application of DNA Quadruplex Hydrogels Prepared from Polyethylene Glycol-Oligodeoxynucleotide Conjugates to Cell Culture Media

Polymers ◽  
2019 ◽  
Vol 11 (10) ◽  
pp. 1607
Author(s):  
Shizuma Tanaka ◽  
Shinsuke Yukami ◽  
Yuhei Hachiro ◽  
Yuichi Ohya ◽  
Akinori Kuzuya
Keyword(s):  
Cell Culture ◽  
High Efficiency ◽  
Culture Media ◽  
Culture Conditions ◽  
Cell Culture Media ◽  
G Quadruplex ◽  
L929 Fibroblast ◽  
Dna Quadruplex ◽  
Quadruplex Formation ◽  
Poly Ethylene

Application of Na+-responsive DNA quadruplex hydrogels, which utilize G-quadruplexes as crosslinking points of poly(ethylene glycol) (PEG) network as cell culture substrate, has been examined. PEG-oligodeoxynucleotide (ODN) conjugate, in which four deoxyguanosine (dG4) residues are tethered to both ends of PEG, was prepared by modified high-efficiency liquid phase (HELP) synthesis of oligonucleotides and used as the macromonomer. When mixed with equal volume of cell culture media, the solution of PEG-ODN turned into stiff hydrogel (G-quadruplex hydrogel) as the result of G-quadruplex formation by the dG4 segments in the presence of Na+. PEG-ODN itself did not show cytotoxicity and the resulting hydrogel was stable enough under cell culture conditions. However, L929 fibroblast cells cultured in G-quadruplex hydrogel remained spherical for a week, yet alive, without proliferation. The cells gradually sedimented through the gel day by day, probably due to the reversible nature of G-quadruplex formation and the resulting slow rearrangement of the macromonomers. Once they reached the bottom glass surface, the cells started to spread and proliferate.

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